BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Yifan Wei,Nana Pan,Jingjing Du,Chao Wang,Xin Chen,Jin-ao Li,Li Lu,Baosheng Ge,Fang Huang,Xiaojuan Wang
The complex synthesis, purification, and delivery of photosensitizers remain major bottlenecks for clinical photodynamic therapy (PDT). Here, we develop PCB@Ecoli@CaP, an engineered Escherichia coli –based living therapeutic that autonomously biosynthesizes the natural photosensitizer phycocyanobilin (PCB) and is encapsulated within a calcium phosphate (CaP) shell to form a self-contained photodynamic system. Under 660 nm laser irradiation, PCB@Ecoli@CaP efficiently generates reactive oxygen species (ROS), which induce potent oxidative stress to eradicate 4T1 tumor cells and simultaneously trigger bacterial self-killing, thereby establishing a self-limiting therapeutic platform. The CaP coating enhances biocompatibility and stability while modulating light penetration and ROS release, as confirmed by DPBF photobleaching, intracellular ROS imaging, and CCK-8 viability measurements. This dual-function system integrates in situ photosensitizer biosynthesis, on-demand photodynamic activation, and built-in safety through self-elimination into a single, programmable microbial platform, offering a simplified, scalable, and safer strategy for next-generation PDT.
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DOI
PubMed
高影响因子
mSphere
·
2025
·Vol. 10
·期 6
·
IF 3.1
Jieying Li,Yuxiao Liu,Jingsi Jiang,Fang Chen,Nan Zhang,Xun Kang,Lin Liu,Yingjuan Wang,Qianfeng Xia,Chuanlong Zhu,Dai Kuang
Klebsiella pneumoniae is a globally recognized microbial pathogen with significant clinical impact. The bacterium harbors the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems, which provide adaptive immunity against invading foreign nucleic acids. Recent studies suggest that certain CRISPR-Cas systems can regulate endogenous genes, influencing bacterial virulence. However, their role in regulating pathogenicity in K. pneumoniae remains poorly understood. This study investigates the regulatory role of the type I-E* CRISPR-Cas system in a hypervirulent K. pneumoniae strain, focusing on its impact on histidine metabolism and pathogenicity. Transcriptome analyses identified differentially expressed genes (DEGs) between the casABECD-deletion and wild-type strains, including significant upregulation of the histidine utilization (Hut) operon and downregulation of biofilm-related genes. These molecular changes resulted in enhanced histidine metabolic activity, reduced biofilm formation, attenuated virulence in A549 lung epithelial cells, and improved survival of Galleria mellonella, as validated through phenotypic and virulence assays. Our bioinformatic analysis indicated that the CRISPR-Cas system in K. pneumoniae targets the hutT sequence, which is part of the Hut operon. Furthermore, the overexpression of hutT mitigated CRISPR-Cas-mediated repression of the Hut operon, as observed in virulence assays, while simultaneous deletion of hutH and casABECD restored the reduced virulence in the ΔcasABECD strain. Additionally, deletion of casABECD significantly enhances the growth of the strain in medium with histidine as the sole carbon source, highlighting the intricate regulatory role of the CRISPR-Cas system in metabolic adaptation. Collectively, these findings uncover a novel role for the CRISPR-Cas system in regulating metabolic pathways and virulence in hypervirulent K. pneumoniae.
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PubMed
高影响因子
JOURNAL OF DAIRY SCIENCE
·
2022
·
IF 4.225
Fan Yang,Xin Ma,Wei Hu,Ziyi Xiong,Meijiang Huang,Yong Wu,Xuanyi Meng,Zhihua Wu,Anshu Yang,Xin Li,Hongbing Chen
ABSTRACT Dairy processing can alter the digestion stability and bioavailability of cow milk proteins in the gastrointestinal tract. However, analysis of stable linear epitopes on cow milk allergens that could enter into intestinal mucosal is limited. Thus, this study aimed to investigate the digestion and transportation properties and residual allergen epitopes entering into gastrointestinal mucosa of 3 commercial dairy products, including pasteurized milk (PM), ultra-heat-treated milk (UHTM), and dried skim milk (DSM). In this work, the digestive stability of the 3 kinds of dairy products has been performed in a standard multistep static digestion model in vitro and characterized by Tricine-SDS-polyacrylamide gel electrophoresis and reversed-phase HPLC. With respect to gastrointestinal digestion in vitro, the main allergens including β-lactoglobulin (β-LG), α-lactalbumin (α-LA), and caseins were degraded gradually, and the resistance peptides remained in the PM with a molecular weight of range from 3.4 to 5.0 kDa. Simultaneously, the potential allergenicity of the cow milk proteins was diminished gradually and is basically consistent after 60 min of gastrointestinal digestion. After gastrointestinal digestion, the remaining peptides were transported via an Ussing chamber and identified by liquid chromatography-MS/MS. By alignment, 10 epitopes peptides were identified from 16 stable peptides, including 5 peptides (AA 92–100, 125–135, 125–138, and 149–162) in β-LG, 2 peptides in α-LA (AA 80–93 and 63–79), 2 peptides in α S1 -casein (AA 84–90 and 125–132), and 1 peptide (AA 25–32) in α S2 -casein were identified by dot-blotting mainly exist in UHTM and PM. This study demonstrates dairy processing can affect the digestion and transport characteristics of milk proteins and in turn alter epitope peptides release.
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PubMed
高影响因子
STAR Protocols
·
2023
·
IF 0
Zhen-Ping Zou,Yu-Hang Fan,Yue Du,Ting-Ting Fang,Wei Du,Ying Zhou,Bang-Ce Ye
Summary Engineered microorganisms hold potential for disease diagnosis and treatment. Here, we present a protocol to engineer E. coli Nissle 1917 strain (iROBOT) using genome insertion and plasmid construction to diagnose, record, and ameliorate inflammatory bowel disease in mice. We describe steps for constructing and administering iROBOT, diagnosing and recording colitis, preparing samples, and analyzing fluorescence and base editing ratios of iROBOT. We detail a colitis ameliorating assay using the disease activity index, colon length, tissue pathological section, and cytokine analysis. For complete details of the use and execution of this protocol, please refer to Zou et al. 1
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DOI
PubMed
Theranostics
·
2017
·
IF 8.766
Jing-Bo Hu,Xu-Qi Kang,Jing Liang,Xiao-Juan Wang,Xiao-Ling Xu,Ping Yang,Xiao-Ying Ying,Sai-Ping Jiang,Yong-Zhong Du
The effective treatment for acute kidney injury (AKI) is currently limited, and care is primarily supportive. Sialic acid (SA) is main component of Sialyl Lewis x antigen on the mammalian cell surface, which participates in E-selectin binding. Therefore, dexamethasone(DXM)-loaded E-selectin-targeting sialic acid-polyethylene glycol-dexamethasone (SA-PEG-DXM/DXM) conjugate micelles are designed for ameliorating AKI. The conjugates are synthesized via the esterification reaction between PEG and SA or DXM, and can spontaneously form micelles in an aqueous solution with a 65.6 µg/mL critical micelle concentration. Free DXM is incorporated into the micelles with 6.28 ± 0.21% drug loading content. In vitro DXM release from SA-PEG-DXM/DXM micelles can be prolonged to 48h. Much more SA-PEG-DXM micelles can be internalized by lipopolysaccharide (LPS)-activated human umbilical vein endothelial cells (HUVECs) in comparison to PEG-DXM micelles due to specific interaction between SA and E-selectin expressed on HUVECs, and consequently more SA-PEG-DXM micelles are accumulated in the kidney of AKI murine model. Furthermore, SA in SA-PEG-DXM conjugates can significantly ameliorate LPS-induced production of pro-inflammatory cytokines via suppressing LPS-activated Beclin-1/Atg5-Atg12-mediated autophagy to attenuate toxicity. Compared with free DXM and PEG-DXM/DXM micelles, SA-PEG-DXM/DXM micelles show better therapeutical effects, as reflected by the improved renal function, histopathological changes, pro-inflammatory cytokines, oxidative stress and expression of apoptotic related proteins.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Lupeng Cui,Yumeng Qiu,Yu Liang,Chunjie Du,Weiliang Dong,Cheng Cheng,Bingfang He
The PET hydrolase from Ideonella sakaiensis ( Is PETase) is efficient for PET degradation, which provides a promising solution for environmental contamination by plastics. This study focuses on improving the excretion of Is PETase from E. coli by signal peptide (SP) engineering. A SP enhancer B1 (MERACVAV) was fused to the N-terminal of commonly-used SP (PelB, MalE, LamB, and OmpA) to mediate excretion of Is PETase. Strikingly, the modified SP B1OmpA, B1PelB, and B1MalE significantly increased the excretion of Is PETase, while Is PETase was basically expressed in periplasmic space without enhancer B1. The excretion efficiency of Is PETase mediated by B1PelB was improved by 62 folds compared to that of PelB. The hydrolysis of PET by crude Is PETase in culture solution was also enhanced. Furthermore, the amount of released MHET/TPA from PET by Is PETase was increased by 2.7 folds with pre-incubation of hydrophobin HFBII. Taken together, this work may provide a feasible strategy for the excretion and application of the Is PETase.
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PubMed
高影响因子
Magnetic Resonance Letters
·
2026
·Vol. 7
·期 3
·200313
·
IF 2.6
Qiang Zhang,Ju Yang,Rong Yan,Yunfei Hu
G protein-coupled receptors (GPCRs) are pivotal therapeutic targets that signal through heterotrimeric G proteins. While structural insights into Gα s and Gα i/o subfamilies have advanced significantly, characterizing the Gα q/11 subfamily remains challenging due to poor soluble expression in prokaryotic systems, precluding high-yield isotope labeling needed for NMR studies. Here, we present an efficient expression strategy for Gα q protein in Escherichia coli . We identified the H5 helix as a critical determinant of folding and solubility. By swapping this helix with its Gα i/o counterpart and employing low-temperature co-expression with bacterial chaperones, we significantly enhance soluble yields while retaining GTPase activity. The 15 N-labeled protein exhibited well-dispersed 1 H– 15 N HSQC spectra, validating the suitability of this expression strategy for future high-resolution NMR investigations. Download: Download high-res image (183KB) Download: Download full-size image
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Chemical Engineering Journal Advances
·
2021
·
IF 0
Chao Liu,Cao Fang,Changsheng Shao,Xinxin Zheng,Hangbo Xu,Qing Huang
Currently, there is a huge demand for medical biomaterials aimed to improve antibacterial effect and promote wound healing. In this work, a facile and single-step approach of synthesizing silver nanoparticles (AgNPs) and reduced graphene oxide (rGO) composite material (namely, AgNPs@rGO) using high-voltage direct-current (DC) non-thermal plasma was proposed. Electron-beam (e-beam) from the DC-plasma discharge zone was implanted into the solution of AgNO 3 and graphene oxide (GO), leading to the reduction of both GO and silver ions. As a result, AgNPs were formed and distributed homogeneously on the reduced graphene sheets. This composite material exhibits excellent antibacterial performance, being able to kill both Gram-positive bacteria Staphylococcus aureus ( S. aureus ) and Gram-negative bacteria Escherichia coli ( E. coli ) effectively. Furthermore, this composite material was utilized to prepare wound-healing band-aids. The in vitro assay on mice confirmed the satisfactory curing effect. The cytotoxicity for bio-compatibility and the mechanism for sterilization were also examined and clarified. As such, this work puts forward a facile and new route of preparing antibacterial biomaterial and demonstrates its promising prospect in wound-healing application.
研究领域:
材料科学
·
生物医学工程
·
纳米医学
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DOI
INDUSTRIAL CROPS AND PRODUCTS
·
2017
·
IF 3.181
Weibin Ma,Luling Zhao,Yanli Xie
The efficacy of eight kinds of green leaf volatiles (GLVs) was investigated as potential natural antifungal agents against Aspergillus flavus in this study. Based on the in vitro assay, volatile phase inhibitory effects of the selected eight GLVs were consistently more effective on A. flavus growth than contact phase inhibitory effects, and ( E )-2-hexenal exhibited the highest efficacy as it could totally inhibited growth of A. flavus at concentrations of 0.032 μL/mL air and 1.6 μL/mL under vapor and contact conditions, respectively. Furthermore, results obtained from the in vivo assay demonstrated that ( E )-2-hexenal possessed potent fumigant activity in reducing A. flavus infection in stored peanut seeds, as it could completely inhibit colonization of A. flavus at 0.32 μL/mL air. Both in vitro and in vivo assay results provided convincing evidence that ( E )-2-hexenal, one of the GLVs that is naturally present in many plants, can potentially be used as a highly efficient and eco-friendly antifungal fumigant to protect peanut seeds from contamination of A. flavus during storage.
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高影响因子
Nano Select
·
2021
·Vol. 3
·期 5
·956-964
·
IF 0
Mengjia Li,Lihua Zhu,Xian Zhang,Chen Wang,Deyu Gao,Jiaheng Han,Chunlei Chen,Hongwei Song,Sai Xu,Cong Chen
Owing to the significant roles in balancing carrier transport, bandgap alignment, and perovskite crystallization, realizing high-quality electron transport layers (ETLs) and their effective electronic contact with the perovskite photoactive layer is crucial for future commercialization of perovskite solar cells (PSCs). Here, a high-quality SnO 2 ETL is firstly deposited at room temperature via the low-cost electron beam evaporation (E-beam) technology to help achieve an impressive PCE of 18.88% for rigid PSCs and a PCE of 15.73% for flexible devices. Then we introduced a novel passivating ligand molecule, 11 Maleimidoundecanoic acids (11MA), to help induce functional defects passivation at the interface of SnO 2 /Perovskite to coordinate with Pb 2+ in perovskite and Sn 4+ in SnO 2 via the functional groups (-C = O, -COOH). As a result, 11MA could help achieve the best PCE of 20.94% with negligible hysteresis for rigid devices. Also, doping 11MA in the perovskite photoactive layer further enhanced the PCE to 22.08% with much improved 2000 hours ambient stabilities. Finally, we fabricated different large-area rigid devices (10, 40, 100, and 600 mm 2 ) toward speeding up the commercialization of PSCs by industrial E-beam technology and rational defects passivation strategies.
研究领域:
材料科学
·
能源技术
·
纳米技术
·
光电工程
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DOI
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xuan Huang,Ming Yi,Kewen Tang
To address resources shortage and environmental degradation, noble metal recovery from e-waste is in desperate need. Herein, to achieve selective extraction of gold (Au) and palladium (Pd) from practical e-waste, a series of tridentate thioether extractants are designed with ester functional groups at both ends of the thioether. By varying the chain length of the ester alkyl, the relationship between extractant structure and performance is further explored. The results indicate that the as-introduced tridentate structure facilitate the Au(III) and Pd(II) extraction, demonstrating a complex mechanism involving both coordination substitution and ion association. Notably, the extractant with the shortest carbon chain length and smallest molecular weight delivers outstanding extraction capacities of 920.2 mg/g for Au(III) and 280.7 mg/g for Pd(II). More importantly, it exhibits superior selectivity (∼100 %) for Au(III) and Pd(II) in complex metal solutions. This work guides the design of thioether-based extractants for sustainable recovery of noble metal from practical e-waste.
研究领域:
化学工程
·
环境科学
·
材料科学
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高影响因子
Food Chemistry-X
·
2026
·
IF 9.2
Guili Huang,Yu Shi,Xinyao Quan,Qianshuo Shao,Su Yan,Siyao Sui,Jiajia Ma,Jiayu Qian,Weiming Chai,Yuning Wang
Volatile Organic Compounds (VOCs) are critical determinants of loquat flavor quality and cultivar differentiation, but comprehensive characterization and rapid detection remain challenging. This study aimed to establish a robust approach for discriminating three loquat cultivars and evaluating their flavor quality by integrating electronic nose (E-nose) technology and Comprehensive Two-Dimensional Gas Chromatography–Time-of-Flight Mass Spectrometry (GC × GC-TOF-MS). GC × GC-TOF-MS was used for VOC profiling, while E-nose was applied for rapid fragrance characterization, with orthogonal partial least squares-discriminant analysis (OPLS-DA) and correlation analysis performed to identify discriminatory compounds and sensor-VOC relationships. GC × GC-TOF-MS identified over 2000 volatile compounds across three loquat cultivars. OPLS-DA further screened 22 discriminatory VOCs with variable importance in projection (VIP) values >1, which contributed to aroma hierarchy shifts among cultivars. E-nose sensors W1W and W1S showed high sensitivity to floral-scent-related VOCs, serving as key factors for distinguishing fragrance quality. Notably, E-nose detection results exhibited good consistency with GC × GC-TOF-MS data, and W1W/W1S sensors were significantly positively correlated with key VOCs. These findings demonstrate that the integration of E-nose and GC × GC-TOF-MS offers complementary advantages for comprehensive VOC characterization and cultivar discrimination. The established approach provides a scientific basis for optimizing loquat quality standards by incorporating VOC-driven biochemical authenticity and a potential efficient method for rapid, non-destructive assessment of postharvest flavor quality in loquat cultivars. These results facilitate quality control and cultivar authentication in the fruit industry.
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PubMed
高影响因子
危险品化学品经营许可证（带存储）