JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Lili Qi,Shun Zhang,Feiqiang Cao,Yuxiang Li,Zhijian Ke,Mengting Wang,Haiguang Mao,Wei Ma,Yong Yu,Jinbo Wang
Resveratrol production via enzymatic hydrolysis of polydatin is hindered by the thermal instability of β-glucosidases. This study employed rational design to engineer Bacillus sp. D1-derived BglD2 through surface charge optimization. The D156E mutant achieved a 42.7% increase in catalytic efficiency (kcat/Km = 49.76 s–1·mM–1) toward polydatin and a 2.1-fold longer half-life at 40 °C. The D411E mutant exhibited a 5.2-fold improvement in thermostability while retaining native activity. Structural analyses revealed that D156E formed a stabilizing salt bridge (2.7 Å) with Lys111 and a hydrogen bond with Pro112, while D411E established compensatory hydrogen bonds with Phe408. Both mutants maintained broad pH activity (pH 6.0–7.0), high glucose tolerance (Ki > 55 mM), and metal ion resilience. This work demonstrates that targeted surface charge engineering concurrently enhances thermostability and catalytic efficiency, enabling efficient resveratrol production with reduced enzyme consumption in industrial applications.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Yuanyuan Chen,Chang Zhang,David Julian McClements,Yanyan Zhou,Xuguang Yin,Runpu Shen,Xin Tang,Kewu Zhu,Xiang Luo
This study developed novel maltodextrin-vitamin E succinate (DE19-VES) conjugates were synthesized at different mass ratios of VES to maltodextrin (5 % and 10 %) as multifunctional emulsifiers for resveratrol encapsulation. Compared to nanoemulsions stabilized by octenyl succinic anhydride-modified starch (OSA S), those stabilized by DE19-VES conjugates exhibited significantly smaller droplet sizes, higher encapsulation efficiencies, and superior physical stability. Crucially, the DE19-VES conjugates imparted exceptional antioxidant properties to the nanoemulsions, enhancing radical scavenging activity, oxidative stability, and markedly improved protection of encapsulated resveratrol against degradation. In vitro digestion demonstrated significantly enhanced resveratrol bioavailability from DE19-VES-stabilized nanoemulsions versus OSA S. Cytotoxicity evaluation using human gastric mucosal epithelial cells (GES-1) confirmed that all formulations were non-toxic. These results highlight the dual functionality (emulsification and potent antioxidant activity) of the engineered maltodextrin conjugates, establishing their strong potential as advanced carbohydrate-based emulsifiers for protecting and delivering sensitive bioactives in pharmaceuticals, functional foods, and supplements.
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ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Changjiang Li,Na Wang,Guodong Zheng,Licong Yang
Alzheimer’s disease (AD) is a neurodegenerative disease associated with amyloid-β (Aβ) deposition, leading to neurotoxicity (oxidative stress and neuroinflammation) and gut microbiota imbalance. Resveratrol (Res) has neuroprotective properties, but its bioavailability in vivo is very low. Herein, we developed a small Res-selenium-peptide nanocomposite to enable the application of Res for eliminating Aβ aggregate-induced neurotoxicity and mitigating gut microbiota disorder in aluminum chloride (AlCl3) and d-galactose(d-gal)-induced AD model mice. Res functional selenium nanoparticles (Res@SeNPs) (8 ± 0.34 nm) were prepared first, after which the surface of Res@SeNPs was decorated with a blood–brain barrier transport peptide (TGN peptide) to generate Res-selenium-peptide nanocomposites (TGN-Res@SeNPs) (14 ± 0.12 nm). Oral administration of TGN-Res@SeNPs improves cognitive disorder through (1) interacting with Aβ and decreasing Aβ aggregation, effectively inhibiting Aβ deposition in the hippocampus; (2) decreasing Aβ-induced reactive oxygen species (ROS) and increasing activity of antioxidation enzymes in PC12 cells and in vivo; (3) down-regulating Aβ-induced neuroinflammation via the nuclear factor kappa B/mitogen-activated protein kinase/Akt signal pathway in BV-2 cells and in vivo; and (4) alleviating gut microbiota disorder, particularly with respect to oxidative stress and inflammatory-related bacteria such as Alistipes, Helicobacter, Rikenella, Desulfovibrio, and Faecalibaculum. Thus, we anticipate that Res-selenium-peptide nanocomposites will offer a new potential strategy for the treatment of AD.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Songqing Huang,Jianying Yang,Shuting Li,You Qin,Qian Mo,Limin Chen,Xinchun Li
A highly sensitive molecular imprinted-based electrochemical sensor for resveratrol is described. Electrodeposition and electropolymerization techniques were successively used to prepare gold nanoparticles (AuNPs) and polyaniline (PANI) film on glassy carbon electrode. The as-formed porous PANI/AuNPs composite acted as a conducting network mediating electron transfer, and offered supporting matrix for anchoring the imprinted elements—polyacrylamide (PAM) that was chemically polymerized at the composite interface using acrylamide as functional monomer and resveratrol as template molecule in an amiable condition, thereby forming a molecular imprinted polymer (MIP) capable of specifically recognizing and electrochemically responding to resveratrol. The preparation conditions were carefully investigated aiming to obtain a high-performance MIP-based sensor for electrochemical sensing of resveratrol. Quantification was performed by differential pulse voltammetry in pH 4.0 phosphate buffer. The peak current was directly proportional to resveratrol concentration ranging from 1.0 μM to 200 μM, with detection limit of 87 nM. A variety of commonly coexisting ingredients such as ferulic acid, catechin, caffeic acid, vanillin, and protocatechin acid cannot produce detectable current signal in the given analytical condition, enabling the direct detection of resveratrol in wines without the requirement of sample pretreatment (e.g., chromatographic separation or solvent extraction).
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 56
·期 4
·1788-1800
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IF 2.773
Huanhuan Dong,Xin Yin,Wusigale,Hao Cheng,Narangerel Choijilsuren,Xing Chen,Li Liang
Summary Ligand-binding proteins were proposed as ideal carriers for the co-encapsulation of bioactive compounds. Multiple compounds co-encapsulated in food formulation may give rise to different effects on bioactivity and stability. The protein effect on the interplay between bioactive compounds was investigated for selecting suitable co-encapsulation strategy. α-Tocopherol, resveratrol and epigallocatechin-3-gallate (EGCG), as model compounds, interacted with bovine serum albumin (BSA) to form tri-ligand complexes when added in the sequence. For ferric-reducing antioxidant power, the mixtures of α-tocopherol and resveratrol,α-tocopherol and EGCG, resveratrol and EGCG were respectively additive, antagonistic and synergistic, while their ternary mixture were antagonistic. For ABTS+ scavenging capacity, all pairs were antagonistic. BSA improved FRAP of α-tocopherol, resveratrol and EGCG mixture but decreased their ABTS inhibition. Although structural stability and antioxidant activity of resveratrol decreased when alone interacted with BSA, they were improved when α-tocopherol and EGCG were co-presented. Mixing the antioxidants improved their stability and the complexation with BSA can further stabilise them. BSA could thus be a suitable carrier for co-encapsulation/protection of α-tocopherol, resveratrol and EGCG in functional foods.
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JOURNAL OF PERIODONTAL RESEARCH
·
2023
·
IF 3.5
Yifan Ma,Yi Qian,Yuteng Chen,Xiaoxu Ruan,Xiaoya Peng,Yi Sun,Jue Zhang,Jingjing Luo,Songlin Zhou,Chao Deng
Objective The purpose of this study was to investigate resveratrol's specific role as an anti-inflammatory and osteogenic differentiation of hPDLSCs in periodontitis and to reveal the mechanisms involved. Background Numerous studies have shown that inhibiting the inflammatory response of periodontal tissues and promoting the regeneration of alveolar bone are crucial treatments for periodontitis. Resveratrol has been found to have certain anti-inflammatory property. However, the anti-inflammatory mechanism and osteogenic effect of resveratrol in periodontitis are poorly understood. Materials and Methods We constructed an in vitro periodontitis model by LPS stimulation of hPDLSCs and performed WB, RT-qPCR, and immunofluorescence to analyze inflammatory factors and related pathways. In addition, we explored the osteogenic ability of resveratrol in in vitro models. Results In vitro, resveratrol ameliorated the inflammatory response associated with activation of the NF-κB pathway through activation of the NRF2/HO-1 pathway, characterized by inhibition of p65/p50 nuclear translocation and the proinflammatory cytokines interleukin-1β levels. Resveratrol also has a positive effect on osteogenic differentiation. Conclusions Observations suggest that resveratrol modulates the inflammatory response in hPDLSCs via the NRF2/HO-1 and NF-κB pathways and promotes osteogenic differentiation.
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ENVIRONMENTAL TOXICOLOGY
·
2023
·
IF 4.5
Song Yu,Lianpeng Zou,Jiawei Zhao,Yiping Zhu
Fumonisins are common contaminants in the global food and environment, pose a variety of health risks to humans and animals. However, the method of mitigating fumonisin toxicity is still unclear. Resveratrol is a natural compound with antioxidant and anti-inflammatory properties. In this study, the protective effect of resveratrol against fumonisin-induced intestinal toxicity was investigated by the porcine intestinal epithelial cell line (IPEC-J2). The cells were treated with 0–40 μM fumonisin for 24 or 48 h with or without the 24 h resveratrol (15 μM) pretreatment. The data showed that resveratrol could alleviate the fumonisin B1 (FB1)-induced decrease in cell viability and amplify in membrane permeability. At the same time, it could reduce the accumulation of intracellular reactive oxygen species and increase the expression ranges of Nrf2 and downstream genes (SOD1 and NQO-1), thereby counteracting FB1-induced apoptosis. Furthermore, resveratrol was able to reduce the expression levels of inflammatory factors (TNF-α, IL-1β, and IL-6), increase the expression levels of tight junction proteins (Claudin-1, Occludin, and ZO-1), and the integrity of the IPEC-J2 monolayer. Our data also showed that resveratrol could attenuate the toxicity of the co-occurrence of three fumonisins. It is implied that resveratrol represents a promising protective approach for fumonisin, even other mycotoxins in the future. This provided a new strategy for further blocking and controlling the toxicity of fumonisin, subsequently avoiding adverse effects on the human and animal health.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 8
·3395
·
IF 4.6
Yi Ou,Zonglin You,Min Yao,Yingfan Cao,Xiu Xue,Min Chen,Rihui Wu,Lishe Gan,Dongli Li,Panpan Wu,Xuetao Xu,Wingleung Wong,Vincent Kam Wai Wong,Wenfeng Liu,Jiming Ye,Jingwei Jin
Naproxen is widely used for anti-inflammatory treatment but it can lead to serious side effects. To improve the anti-inflammatory activity and safety, a novel naproxen derivative containing cinnamic acid (NDC) was synthesized and used in combination with resveratrol. The results showed that the combination of NDC and resveratrol at different ratios have a synergistic anti-inflammatory efficacy in RAW264.7 macrophage cells. It was indicated that the combination of NDC and resveratrol at a ratio of 2:1 significantly inhibited the expression of carbon monoxide (NO), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), induced nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) and reactive oxygen species (ROS) without detectable side effects on cell viability. Further studies revealed that these anti-inflammatory effects were mediated by the activation of nuclear factor kappa-B (NF-κB), mitogen-activated protein kinase (MAPK) and phosphoinositide-3 kinase (PI3K)/protein kinase B (Akt) signaling pathways, respectively. Taken together, these results highlighted the synergistic NDC and resveratrol anti-inflammatory activity that could be further explored as a strategy for the treatment of inflammatory disease with an improved safety profile.Keywords:naproxen derivative;resveratrol;synergistic;anti-inflammatory;mechanism
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CANCER SCIENCE
·
2018
·Vol. 109
·期 9
·2677-2686
·
IF 4.372
Yilin Zhao,Qi Shao,Haitao Zhu,Haiyuan Xu,Wei Long,Bin Yu,Lining Zhou,Huaxi Xu,Yan Wu,Zhaoliang Su
Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of immature myeloid cells which consist of 2 subsets: granulocytic MDSC (G-MDSC) and monocytic MDSC (M-MDSC). MDSC expand in tumor-bearing hosts and contribute to immunotherapeutic resistance by remarkably blocking effector T-cell activation via different mechanisms. Resveratrol (RSV) is a polyphenol and it has been widely used for its various health benefits. However, the underlying mechanism of its anti-tumor properties remains unclear. In this study, a transplantable mouse model was used to investigate the effects of RSV on MDSC. The results showed that RSV ameliorated tumor development by decreasing G-MDSC accumulation, impairing its suppressive ability on CD8 + T cells and promoting M-MDSC differentiation into CD11c + and F4/80 + cells. Our results indicated that RSV should be considered as a modular of MDSC suppressive function and that RSV is a novel booster for tumor immunotherapy.
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FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Taotao Dai,Ruyi Li,Chengmei Liu,Wei Liu,Ti Li,Jun Chen,Mahesh Kharat,David Julian McClements
An understanding of the molecular mechanisms underpinning protein-polyphenol interactions is critical for incorporating bioactive polyphenols into functional foods and beverages. This study characterized the interaction between rice glutelin (RG) and resveratrol (RES) using a combination of spectroscopic and molecular simulation techniques. Resveratrol quenched the intrinsic fluorescence of rice glutelin, which is indicative of a binding interaction between the two molecules. Resveratrol addition caused a decrease in surface hydrophobicity, alteration in protein microenvironment, and change in conformation of the rice glutelin. Thermodynamic analysis suggested that the binding of resveratrol to the protein was spontaneous and mainly driven by hydrophobic interactions. A RG-RES complex with a molar ratio of 1:1 was formed, suggesting that rice glutelin may be a suitable carrier for resveratrol. More detailed insights into the nature of the binding site and the forces involved were obtained using molecular simulation studies. Finally, we examined the impact of resveratrol on the formation and stability of oil-in-water emulsions stabilized by rice glutelin. Emulsions containing small highly anionic oil droplets could be formed in the presence of resveratrol, but a population of large particles was observed during storage. These particles appeared to consist of oil droplets with water droplets trapped inside. We hypothesize that water diffused into the oil droplets and formed small water droplets that were stabilized by amphiphilic resveratrol. Our results have important implications for the development of effective delivery systems for resveratrol.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yong Tang,Xiyu Zhang,Zheng Liu,Xiaoya Liu,Xiaojie Li
Epoxy resin is renowned for its admirable comprehensive properties, but its inherent brittleness and poor fire safety severely limit its practical application in high-tech fields. Therefore, improving the fire safety and toughness of epoxy thermosets while maintaining their strength and thermal stability is both urgent and challenging. In this study, a novel hyperbranched polymer (HBPDR-DPP) has been synthesized via a one-pot, two-step process using dichlorodiphenylsilane, resveratrol, and diphenylphosphinic chloride. This polymer was then incorporated into high-performance epoxy thermosets. The resulting thermoset, containing 4 wt% HBPDR-DPP, exhibited an impressive limiting oxygen index (LOI) of 30.2 %, along with a 26.0 % decrease in peak heat release rate (PHRR) and a 10.9 % reduction in total smoke production (TSP) compared to the unmodified epoxy thermoset. These notable improvements can be primarily attributed to the synergistic carbonization effects of the silicon- and phosphorus-containing segments in HBPDR-DPP. Meanwhile, the 4 wt% HBPDR-DPP-modified epoxy thermoset exhibited significant improvements in mechanical properties, with tensile strength, flexural strength, and impact strength increasing by 37.8 %, 54.6 %, and 83.4 %, respectively. These enhancements can be attributed to the hyperbranched structure and the combination of rigid aromatic units with flexible Si-O chains in HBPDR-DPP. Moreover, the EP/HBPDR-DPP thermoset demonstrated excellent thermal stability. This study presents a simple and effective strategy for enhancing both the fire safety and mechanical properties of epoxy thermosets, offering valuable insights for the development of high-performance thermosetting materials.
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RSC Advances
·
2024
·Vol. 14
·期 43
·31768-31776
·
IF 3.9
Jiawei Zhao,Li Lu,Qiwei Chai,Wei Jin,Min Zhu,Shengqi Qi,Jiali Shentu,Yuyang Long,Dongsheng Shen
The unique capacity of certain plant endophytes to degrade organic pollutants has garnered considerable interest in recent years. However, it remains uncertain whether endophytes can maintain high degradation activity after in vitro culture and whether they can be used directly in the remediation of contaminated soils. This study reveals that resveratrol, a plant secondary metabolite, selectively boosts the degradation of polycyclic aromatic hydrocarbons (PAHs) by endophytic Methylobacterium extorquens C1 (C1) in vitro, while exerting negligible effects on the activity of indigenous soil bacteria. For the first time, a combined application of C1 and resveratrol was employed in the remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil. The findings indicate that the sole use of resveratrol failed to promote the removal of PAHs by indigenous soil microorganisms, whereas sole application of C1 boosted Methylobacterium-related PAH-degrading bacterial abundance, enhancing PAH removal, yet concurrently reduced overall soil microbial diversity. The combination of resveratrol and C1 not only stimulated the PAH removal but also mitigated the impact of C1 on the soil microbial community structure when C1 was applied individually. Specifically, the optimal removal efficacy was achieved with a treatment combination of 5 mg kg−1 resveratrol and 1.2 × 107 CFU kg−1 of C1, leading to a 130% and 231% increase in the removal of phenanthrene and acenaphthene, respectively, over a 15 days period. This study proposes a novel approach for the bioremediation of organic-contaminated soil by using the specific biological response of plant endophytic bacteria to secondary metabolites.
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危险品化学品经营许可证（带存储）