Current Medical Science
·
2023
·Vol. 43
·期 2
·223-231
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IF 2.4
Zhang Qing,Pan Rong-rong,Wu Yu-tao,Wei Yu-miao
Objective Vascular smooth muscle cell (VSMC) differentiation from stem cells is one source of the increasing number of VSMCs that are involved in vascular remodeling-related diseases such as hypertension, atherosclerosis, and restenosis. MicroRNA-146a (miR-146a) has been proven to be involved in cell proliferation, migration, and tumor metabolism. However, little is known about the functional role of miR-146a in VSMC differentiation from embryonic stem cells (ESCs). This study aimed to determine the role of miR-146a in VSMC differentiation from ESCs. Methods Mouse ESCs were differentiated into VSMCs, and the cell extracts were analyzed by Western blotting and RT-qPCR. In addition, luciferase reporter assays using ESCs transfected with miR-146a/mimic and plasmids were performed. Finally, C57BL/6J female mice were injected with mimic or miR-146a-overexpressing ESCs, and immunohistochemistry, Western blotting, and RT-qPCR assays were carried out on tissue samples from these mice. Results miR-146a was significantly upregulated during VSMC differentiation, accompanied with the VSMC-specific marker genes smooth muscle-alpha-actin (SMαA), smooth muscle 22 (SM22), smooth muscle myosin heavy chain (SMMHC), and h1-calponin. Furthermore, overexpression of miR-146a enhanced the differentiation process in vitro and in vivo . Concurrently, the expression of Kruppel-like factor 4 (KLF4), predicted as one of the top targets of miR-146a, was sharply decreased in miR-146a-overexpressing ESCs. Importantly, inhibiting KLF4 expression enhanced the VSMC-specific gene expression induced by miR-146a overexpression in differentiating ESCs. In addition, miR-146a upregulated the mRNA expression levels and transcriptional activity of VSMC differentiation-related transcription factors, including serum response factor (SRF) and myocyte enhancer factor 2c (MEF-2c). Conclusion Our data support that miR-146a promotes ESC-VSMC differentiation through regulating KLF4 and modulating the transcription factor activity of VSMCs.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 5
·1-11
·
IF 5.7
Dang Meng,Li Zizhe,Mao Yexuan,Huang Xianqing,Song Lianjun,Li Wenfeng,Ma Ruxiang,Liu Yang,Wang Liye,Yu Xuezhi,Yang Huijuan,Zhang Xiya
To meet high-throughput screening of the residues of sulfonamides (SAs) with high sensitivity toward sulfamethazine (SM2) in milk samples, a new highly sensitive lateral flow immunoassay (LFA) based on amorphous carbon nanoparticles (ACNs) was developed. First, a group-specific monoclonal antibody 10H7 (mAb 10H7) that could recognize 25 SAs with high sensitivity toward SM2 (IC 50 value of 0.18 ng/mL) was prepared based on H1 as an immune hapten and H4 as a heterologous coating hapten. Then, mAb 10H7 was conjugated to ACNs as an immune probe for LFA development. Under the optimized conditions, the LFA could detect 25 SAs with the cut-off value toward SM2 of 2 ng/mL, which could meet the requirement for detection of SAs. In addition, the LFA developed was also used for screening SAs’ residues in real milk samples, with results being consistent with HPLC–MS/MS. Thus, this LFA can be used as a high-throughput screening tool for detection of SAs. Graphical abstract
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Nano Research
·
2023
·
IF 9.9
Zhang Yufei,Cheng Qian,Xue Yuhao,Yao Kai,Syeda Madiha Zahra,Xu Jian,Wu Jianheng,Wang Zhenjie,Tang Longguang,Mu Qingchun
Glioma is the most common primary craniocerebral tumor caused by the cancerous growth of glial cells in the brain and spinal cord. Currently, standard treatment is the surgical resection followed by concurrent radiation and chemotherapy. However, the blood-brain barrier (BBB) prevents most antitumor drugs from entering the brain and reduces their efficacy, especially in low-grade glioma. Since L-type amino acid transporter 1 (LAT1) is highly expressed in glioma cells and mediates drug transport across the BBB, it is a promising target for drug delivery and treatment of glioma. Temozolomide (TMZ) is the first-line treatment for glioma, however, patients often exhibit drug resistance at advanced stage. A multikinase inhibitor and inducer of ferroptosis, sorafenib can improve the therapeutic effects of TMZ. Therefore, to optimize the glioma treatment and cross the BBB, we designed LAT1-targeting nanoparticles co-loaded with TMZ and sorafenib. Our results from both in vitro and in vivo studies confirmed that LAT1-targeting nanoparticles significantly increased the cellular uptake, cytotoxicity, accumulation at tumor site, and the anti-tumor efficacy compared to the non-target nanoparticles. Therefore, LAT1 can be used as a potential target for brain-targeted drug delivery, and sorafenib-induced ferroptosis can aid the anti-glioma efficacy of TMZ.
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BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
·
2023
·
IF 1.2
He Yuntao,Guo Xiaxin,Qiao Luyang,Ye Runping,Zong Shanshan,Cao Xinyi,Cheng Jiankai,Zhou Zhangfeng,Yao Yuangen
Rh complexes are considered as the most promising catalysts for formaldehyde hydroformylation since they can achieve higher yields of glycolaldehyde under mild conditions. Although heterogeneous catalysts are convenient to separate, Rh-based heterogeneous catalysts usually have limited catalytic activity in hydroformylation of formaldehyde. Here, four different supports (Al 2 O 3 , SiO 2 , SBA-15 and HMS), loaded with Rh by impregnation method, have been developed for formaldehyde hydroformylation. The physico-chemical properties of the materials have been investigated by means of complementary techniques. Due to the presence of more oxidized rhodium species and stronger metal support interactions, the silica-based catalysts achieved higher formaldehyde conversion and glycolaldehyde selectivity than Rh/Al 2 O 3 . Moreover, SBA-15 and HMS zeolites were synthesized by adjusting the structure of the silica support. Thus, the selectivity of 94.8% for glycolaldehyde and a remarkable glycolaldehyde yield of 52.7% over Rh/SBA-15 catalyst was obtained due to its larger surface area, suitable pore structures and higher dispersion of Rh species.
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Biomass Conversion and Biorefinery
·
2023
·
IF 4
Lu Yanyu,Lin Changqu,Yang Hao,Ma Peipei,Chen Jishuang,He Zhuang,Wu Hongli,Cao Fei,Chen Kequan,Ouyang Pingkai
Abstract FDCA (2,5-furandicarboxylic acid), produced by oxidizing carbohydrate-derived HMF (5-hydroxymethylfurfural), is a valuable monomer for biopolymers. The difference in the solvent system between the dehydration reaction and oxidation reactions seriously affects the process continuity and production efficiency. Herein, we reported a cascade process for FDCA production from carbohydrates in a single acetic acid system. The fructose conversion was complete and total furan compounds yield reached nearly 90%, which contained 42.8% HMF and 47.1% 5-acetoxymethylfurfural (AMF) in the acetic acid system with NaBr addition. After the azeotropic distillation and vacuum distillation in situ, AMF, a more stable intermediate than HMF, could be easily collected with 90.5% recovery and 99.9% purity. AMF was also oxidized into FDCA with 91.1% yield by Co/Mn/Br catalysts in the acetic acid system. Finally, the cascade process, including fructose dehydration–esterification and HMF/AMF mixture oxidation, was successfully realized in a single acetic acid system. This process is simple to operate, easy to implement, eliminates the use of extra acid or self-made catalysts and expensive solvents, and has the potential for continuous preparation from carbohydrates to FDCA. Graphical Abstract
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-13
·
IF 16.6
Zhou Rui,Sui Laizhi,Liu Xinbao,Liu Kaikai,Guo Dengyang,Zhao Wenbo,Song Shiyu,Lv Chaofan,Chen Shu,Jiang Tianci,Cheng Zhe,Meng Sheng,Shan Chongxin
Multiphoton excited luminescence is of paramount importance in the field of optical detection and biological photonics. Self-trapped exciton (STE) emission with self-absorption-free advantages provide a choice for multiphoton excited luminescence. Herein, multiphoton excited singlet/triplet mixed STE emission with a large full width at half-maximum (617 meV) and Stokes shift (1.29 eV) has been demonstrated in single-crystalline ZnO nanocrystals. Temperature dependent steady state, transient state and time-resolved electron spin resonance spectra demonstrate a mixture of singlet (63%) and triplet (37%) mixed STE emission, which contributes to a high photoluminescence quantum yield (60.5%). First-principles calculations suggest 48.34 meV energy per exciton stored by phonons in the distorted lattice of excited states, and 58 meV singlet-triplet splitting energy for the nanocrystals being consistent with the experimental measurements. The model clarifies long and controversial debates on ZnO emission in visible region, and the multiphoton excited singlet/triplet mixed STE emission is also observed.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Xiong Xueru,Chen Yunhua,Wang Zhenxing,Liu Huan,Le Mengqi,Lin Caihong,Wu Gang,Wang Lin,Shi Xuetao,Jia Yong-Guang,Zhao Yanli
While hydrogels enable a variety of applications in wearable sensors and electronic skins, they are susceptible to fatigue fracture during cyclic deformations owing to their inefficient fatigue resistance. Herein, acrylated β-cyclodextrin with bile acid is self-assembled into a polymerizable pseudorotaxane via precise host-guest recognition, which is photopolymerized with acrylamide to obtain conductive polymerizable rotaxane hydrogels (PR-Gel). The topological networks of PR-Gel enable all desirable properties in this system due to the large conformational freedom of the mobile junctions, including the excellent stretchability along with superior fatigue resistance. PR-Gel based strain sensor can sensitively detect and distinguish large body motions and subtle muscle movements. The three-dimensional printing fabricated sensors of PR-Gel exhibit high resolution and altitude complexity, and real-time human electrocardiogram signals are detected with high repeating stability. PR-Gel can self-heal in air, and has highly repeatable adhesion to human skin, demonstrating its great potential in wearable sensors.
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JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
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2023
·
IF 2.3
Hong Xiaozhe,Zheng Zhijun,Gao Yan
Although a large number of bionic superhydrophobic surfaces have been reported in recent years, their poor robustness remains to be improved. Herein, we present a method to fabricate a hierarchical micro-nanostructure on Ti6Al4V with excellent robustness by combining laser direct writing technology and hydrothermal treatment. Microscale pore array pattern with hard oxide layer was generated by laser irradiation, which provided protection for the nanowires later grown in situ by hydrothermal treatment. After low surface energy molecular modification, the composite surface exhibited outstanding wetting properties with a contact angle of 165.2° and a roll-off angle less than 1°. We focused on the mechanical and chemical stability of the as-prepared superhydrophobic surface (SHS), and the performance tests included water jet impact, cyclic friction, solution immersion, heat treatment and UV irradiation. Additionally, the SHS was transformed into slippery liquid-infused porous surface (SLIPS) by lubricant injection, which expends the applications of the hierarchical micro-nanostructure surface. Considering the loss degree and the self-repairing performance of the lubricants with different viscosities, we can conclude that the lubricant with a viscosity of 350 mPa·s matches the SHS substrate best. The constructed SHS and SLIPS show remarkable robustness and versatility and may be applied in various fields.
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PLANT AND SOIL
·
2023
·
IF 4.9
Xue Shengguo,Zhang Yufei,Jiang Jun,Li Feng,Chen Chaorong,Guo Xuyao,Chen Qi,Zhu Feng,Chen Hualin,Wu Yujun
Purpose The interaction of minerals and organic matter significantly affects the stability of organic carbon, which is key to the rehabilitation of bauxite residue. However, due to the microscopic structure of minerals and the chemical composition of dissolved organic matter (DOM), the interaction mechanism between alkaline minerals and DOM in the presence of calcium ions that are widely used as amendments is still poorly understood. Methods In this work, the mechanism of adsorption and immobilization of organic matter by sodalite and cancrinite in the presence of calcium ions was investigated at the microscopic scale, using Fourier transform infrared spectroscopy (FTIR), excitation-emission matrix fluorescence (EEM) spectra, thermogravimetric analysis (TG) and atomic force microscope (AFM). Results The calcium ions can act as bridges to connect the negative sites on the mineral surface with the polysaccharide functional groups of organic matter, thus promoting organic carbon sequestration via ligand exchange and/or electrostatic interactions. Furthermore, DOM located in the structure within the cancrinite surface may be better protected due to physical isolation. The 3D view of the interacted minerals-organic matter assemblages provided visual evidence that the adsorption sites distributed on the surface of cancrinite increased by the action of calcium ions, with different adsorption capacities of individual sites for DOM. Conclusions The calcium ions enhance the ability of alkaline minerals to trap dissolved organic carbon, and the work highlighted the significance of organo-mineral associations in the microscopic mineral structure for the organic carbon sequestration in the revegetation process of bauxite residue disposal areas.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2023
·Vol. 30
·期 21
·59554-59566
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IF 5.8
Ma Xiao-Yan,Xie Qing-Qing,Hadiya Ablat,Xamsiya Nurmamat,Zhao Zhi-Xi
Dimethyl arsonic acid, the most common organic arsenic pollutant, is widely present in the environment and seriously threatens the safety of drinking water. Syntheses of magnetite, magnetic bentonite, and magnetic ferrihydrite via hydrothermal methods, and the magnetic composites were examined using XRD, BET, VSM, and SEM. SEM images revealed that many monodispersible pellets were attached to the surface of magnetic bentonite. The magnetic ferrihydrite contained abundant pores and had a rich pore structure, which expanded the specific surface area of the original magnetite. The specific surface areas of the magnetic bentonite and magnetic ferrihydrite were 65.17 and 220.30 m 2 ·g −1 , respectively. The adsorption kinetics and adsorption isotherms of dimethyl arsonic acid on magnetic composites were studied. The adsorption of dimethyl arsonic acid on the magnetic composites conformed to the pseudo-second-order model and Freundlich isothermal adsorption model. By comparing the isotherms of the adsorption of dimethyl arsonic acid by the magnetic composites at pH values of 3, 7, and 11, respectively, it was found that the adsorption of dimethyl arsonic acid was the greatest at neutral pH of 7. The adsorption mechanism was analyzed via zeta potential determination, Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). The zeta potential results revealed that magnetic bentonite electrostatic activity occurred with dimethyl arsonic acid, and the magnetic ferrihydrite indicated a coordination complex with dimethyl arsonic acid. The XPS results revealed that the Fe–O bonds on the surfaces of the magnetic ferrihydrite had coordination complexation effects on the As-O of the dimethyl arsonic acid.
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JOURNAL OF FLUORESCENCE
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2023
·Vol. 33
·期 5
·2015-2021
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IF 2.7
Qin Danni,Han Yaqi,Hu Liang
Coumarin 3-carboxylic acid (CCA)-loaded radio-fluorescent hydrogels have attracted interest for ionizing radiation dosimeters, but their sensitivity needs to be improved. In this study, we added ammonium persulfate (APS) to a polyacrylamide (PAAm)-CCA hydrogel. The introduction of APS improved the hydrogel dose sensitivity to 336.02 Gy − 1 , which is 1.8 times that of the counterpart without APS. Our hydrogel can measure the X-ray dose in a range of 0 − 15 Gy with a lower limit of detection (LOD) of 0.1 Gy. Additionally, the hydrogel can sense X-ray doses within a wide range of the dose rate and temperature, and the dose‒response can be well retained 7 days postirradiation. Therefore, we think this study provides a simple and robust method to improve the sensitivity of CCA hydrogel dosimeters, presenting great potential in clinical radiotherapy.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 12
·5552-5568
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IF 4.5
Gao Zheng,Huang Chao,Tang Ruizhi,Ma Shujuan,Gong Bolin,Ou Junjie
Asymmetric particles (Janus particles) with discrete surface regions owning different chemical compositions or physical shapes have attracted increasing attention in the last two decades. Exploring novel strategies for synthesis of Janus particles is a great challenge due to somewhat complicated process and taking long time. In this work, several micron-scale Janus particles were rapidly prepared by photo-initiated seeded swelling polymerization for the first time, using glycyl methacrylate (GMA) as monomer and pentaerythritol tetraacrylate (PET4A) as crosslinker. After swelling of the seeds, the polymerization could be completed with ultraviolet light of 365 nm for 15 min. The effects of seed amount, molar ratio of monomer to crosslinker, volume ratio and type of porogenic agent and illumination time on Janus particle morphology were systematically investigated, and the resulting Janus particles with semi-raspberry-like morphology could be adjusted. Additionally, Janus particles were modified with a layer of polymer brush by surface-initiated atom transfer radical polymerization. The as-synthesized Janus@poly(GMA)-EDA was utilized for the adsorption of Cu(II) ion, and the adsorption capacity reached 349.6 mg g −1 , indicating that Janus@poly(GMA)-EDA exhibited great application potential for heavy metal removal. Graphical abstract
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危险品化学品经营许可证（带存储）