Frontiers in Behavioral Neuroscience
·
2023
·
IF 3
Shou-Min Xuan,Ya-Wen Su,Yi-Meng Liang,Zhen-Jie Gao,Chun-Yan Liu,Bu-Fang Fan,Yan-Wei Shi,Xiao-Guang Wang,Hu Zhao
Introduction Fear memory generalization is regarded as the core characteristic of posttraumatic stress disorder (PTSD) development. However, the mechanism that contributes to the generalization of conditioned fear memory is still unclear. The generalization is generally considered to be a mismatch that occurs during memory consolidation. Methods Foot shocks and tones were given as unconditioned stress and conditioned stress, respectively for fear conditioning training. Immunofluorescence staining, western blotting and qPCR were performed to determine the expression of different genes in amygdala of mice after fear conditioning training. Cycloheximide was used as a protein synthesis inhibitor and 2-methyl-6-phenylethynyl-pyridine was injected for mGluR5 inhibition. Results Fear conditioning using caused incremental generalization, which was clearly observed during training. The density of c-Fos + cells or the synaptic p-NMDAR expression did not differ with stress intensities. Strong-shock fear conditioning could induce significant mGluR5 de novo synthesis in the amygdala, which was not observed in the weak-shock group. Inhibition of mGluR5 impaired fear memory generalization induced by strong-shock fear conditioning, but the generalization level induced by weak-shock training was enhanced. Discussion These results indicated that mGluR5 in the amygdala is critical to the function of inappropriate fear memory generalization and suggested that this may be a potential target for the treatment of PTSD.
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高影响因子
Biomaterials Science
·
2017
·Vol. 5
·期 11
·2251-2267
·
IF 4.21
Jie Wu,Kunxi Zhang,Xi Yu,Jianxun Ding,Lei Cui,Jingbo Yin
The key barrier to the clinical application of tissue engineering scaffolds is the limitation of rapid and sufficient vascularization. Adipose-derived stem cells (ASCs), especially multicellular aggregates, exhibited a promising angiogenic activity. Herein, we designed a series of poly(L-glutamic acid) (PLGA)-based hydrogels with tunable hydration to control the in situ formation of multicellular spheroids. Oligo(ethylene glycol)s (OEGs) were employed to regulate the hydration of hydrogels. The hydrogel cross-linked with ethylene glycol (OEG1) supported the most excellent adhesion and proliferation of human ASCs in vitro. However, as the hydration of hydrogels strengthened, the adherent ASCs were gradually replaced with multicellular spheroids. Moreover, the in situ formation of spheroids was more effective in upregulating hypoxia-adaptive signals (e.g., hypoxia-inducible factor-1α, HIF-1α) and enhancing the secretion of angiogenic factors (e.g., vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF-2)) compared to adherent cells in OEG1 hydrogels. The hydrogel cross-linked with oligo(ethylene glycol)400 (OEG9) carrying spheroids induced a high angiogenic response of host tissue in vivo, resulting in an improved system vascularization, compared to adherent cells in the OEG1 hydrogel. These features indicated that the PLGA-based hydrogels were expected to be applied toward bone and fat tissue regeneration.
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高影响因子
HUMAN REPRODUCTION
·
2018
·Vol. 33
·期 4
·607-616
·
IF 4.99
Yuan Ming,Li Dong,Zhang Zhe,Sun Huihui,An Min,Wang Guoyun
STUDY QUESTIONWhat happens to the gut microbiota during development of murine endometriosis?SUMMARY ANSWERMice with the persistence of endometrial lesions for 42 days develop a distinct composition of gut microbiota.WHAT IS KNOWN ALREADYDisorders in the immune system play fundamental roles in changing the intestinal microbiota. No study has used high-throughput DNA sequencing to show how endometriosis changes the gut microbiota, although endometriosis is accompanied by abnormal cytokine expression and immune cell dysfunction.STUDY DESIGN, SIZE, DURATIONThis study includes a prospective and randomized experiment on an animal endometriosis model induced via the intraperitoneal injection of endometrial tissues.PARTICIPANTS/MATERIALS, SETTING, METHODSThe mice were divided into endometriosis and mock groups and were sacrificed at four different time points for model confirmation and fecal sample collection. To detect gut microbiota, 16S ribosomal-RNA gene sequencing was performed. Alpha diversity was used to analyze the complexity and species diversity of the samples through six indices. Beta diversity analysis was utilized to evaluate the differences in species complexity. Principal coordinate analysis and unweighted pair-group method with arithmetic means clustering were performed to determine the clustering features. The microbial features differentiating the fecal microbiota were characterized by linear discriminant analysis effect size method.MAIN RESULTS AND THE ROLE OF CHANCEThe endometriosis and mock mice shared similar diversity and richness of gut microbiota. However, different compositions of gut microbiota were detected 42 days after the modeling. Among the discriminative concrete features, the Firmicutes/Bacteroidetes ratio was elevated in mice with endometriosis, indicating that endometriosis may induce dysbiosis. Bifidobacterium, which is known as a commonly used probiotic, was also increased in mice with endometriosis.LARGE SCALE DATAN/A.LIMITATIONS, REASONS FOR CAUTIONMore control groups should be further studied to clarify the specificity of the dysbiosis induced by endometriosis. This study was performed only on mice. Thus, additional data acquired from patients with endometriosis are needed in future research. We only detected the changes of gut microbiota at 42 days after the modeling, while the long-term effect of endometriosis on gut microbiota remains poorly understood. Moreover, we only revealed a single effect of endometriosis on gut microbiota.WIDER IMPLICATIONS OF THE FINDINGSThis study provided the first comprehensive data on the association of endometriosis and gut microbiota from high-throughput sequencing technology. The gut microbiota changed with the development of endometriosis in a murine model. The communication between the host and the gut microbiota is bidirectional, and further studies should be performed to clarify their relationship.STUDY FUNDING/COMPETING INTEREST(S)This research was supported by Grant (81571417) from the National Science Foundation of China and Grant (2015GSF118092) from the Technology Development Plan of Shandong Province. The authors report no conflict of interest.
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KEY ENGINEERING MATERIALS
·
2012
·
IF 0
Yang Hou,Ping Hu,Wen Bo Han
The dispersion of SiC nanopowders in ethanol solution was studied by sedimentation test, particle size measurement and TEM analysis. The dispersion behavior of SiC nanopowders in ethanol solution was strongly dependent on the pH values, types and amounts of dispersant. PEI was found to be effective for the dispersion of SiC nanopowders in ethanol solution. With the addition of PEI, the isoelectric points of SiC nanopowders in ethanol solution were at pH 9.5, and shift to pH 12.3. The stability of SiC suspension increased with the dispersant content increasing until reached 2.5 wt% PEI. The suitable pH value for the dispersion of SiC nanopowders should lower than 10.
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DOI
Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 5
·1747-1753
·
IF 6.4
Xiaowei Zheng,Yao Cheng,Yan Gao,Tao Hu,Ju Xu,Hang Lin,Yuansheng Wang
Dual-response mechanoluminescence (ML) enables light emission upon both the application and withdrawal of mechanical stress, which can provide novel opportunities for stress-sensing. Herein, we present the ML performance of doped ZnGa2O4–PDMS composites with a focus on the dual-response ML behavior upon the dynamic stretching–releasing cycle. Specifically, it was found that the dual-response ML behavior of ZnGa2O4 is doping dependent, which was further proved to be related to doping induced trap distribution. Finally, ZnGa2O4:Mn/PDMS with dual-response ML was used as an electronic skin to demonstrate its unique potential for stress-sensing applications.
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高影响因子
Journal of Flow Chemistry
·
2021
·Vol. 12
·期 1
·41-49
·
IF 2.786
Lan Zhou,Lu Yangcheng
Benzyl iodide, an important organic synthesis intermediate, has high reaction activity as well as high commercial value due to its weakest carbon-halogen bond. However, the development of direct iodination of toluene for benzyl iodide is limited by the fact that the C-I bond is easily decomposed under heat or light. In this work, the photoiodination of toluene to synthesize benzyl iodide in the I 2 /PhI(OAc) 2 / t BuOH system was carefully investigated in the flow photoreactor under LEDs irradiation. The reaction window suitable for the reaction kinetic characteristics recognition was revealed with the considerations of the effects of various parameters including light color, light intensity, reaction temperature, residence time, and usage of each substrate. And an optimized result is to achieve 95.83 % of yield and over 99 % of selectivity under a red light with 2.05 mW/cm 2 of light intensity at 80 o C after 15 min reaction. The reaction kinetic model coupling the iodination reaction (photo and thermal reaction) with the decomposition reaction is established, which fits well the experimental results and provides fundamentals for process design and optimization. Graphical abstract
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高影响因子
Soft Matter
·
2015
·Vol. 11
·期 29
·5940-5946
·
IF 4.029
Wangqin Song,Lvdan Liu,Guangming Liu
Macromolecular crowding plays a significant role in the solubility and stability of biomacromolecules. In this work, the thermo-sensitive poly(N-isopropylacrylamide) (PNIPAM) has been employed as a model system to study the specific ion effects on the solubility of macromolecules in crowded environments of dextran and polyethylene glycol (PEG). Our study demonstrates that crowding agents can interact with either anions or PNIPAM chains. The chaotropic anion SCN− interacts with dextran but does not interact with PEG. Both Cl− and CH3COO− do not interact with dextran and PEG. On the other hand, dextran can interact with PNIPAM as a hydrogen-bond donor, whereas PEG interacts with PNIPAM as a hydrogen-bond acceptor. The salting-in effect exerted by SCN− on PNIPAM is weakened in the crowded environment of dextran but is strengthened in the crowded environment of PEG due to the distinct anion-crowder interactions. In parallel, the salting-out effect generated by Cl− and CH3COO− on PNIPAM is weakened by the crowding of dextran but is strengthened by the crowding of PEG because of the different macromolecule–crowder interactions. Our study reveals that the ion specificity of macromolecules is altered significantly changing from dilute solutions to crowded environments.
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高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2012
·Vol. 95
·期 10
·3214-3219
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IF 2.272
Haoyi Wu,Yihua Hu,Fengwen Kang,Nana Li,Guifang Ju,Zhongfei Mu,Zhongfu Yang
Praseodymium (Pr 3+ ) with rich f energy levels can generate various visible emissions, but the luminescence of Pr 3+ in a CaWO 4 matrix has not yet been studied. In this article, the phosphors of Pr 3+ -doped CaWO 4 were synthesized via a high temperature solid-state reaction, and the photoluminescence (PL) and afterglow properties of Pr 3+ in the CaWO 4 matrix were investigated. The phase identifications of the samples show that an impure monoclinic phase appears when the Pr 3+ concentration is high. The results of PL exhibit the usual Pr 3+ characteristic emissions as well as an emission that is produced by the charge transfer from 5 d of W 6+ to 2 p of O 2− under a shortwave ultraviolet excitation. An intermediate energy transfer occurs during the charge transfer which induces the afterglow. The quenching point of Pr 3+ in CaWO 4 is 2 mol%, corresponding to the critical energy transfer distance of ~20 Å. The quenching mechanism in the Pr 3+ -doped CaWO 4 is the dipole-dipole interaction. The Ca 2+ vacancies ( V Ca ) are the defects that induce the traps and capture holes from O 2− . The thermal energy of holes liberation from V Ca to O 2− results in the charge transfer accompanied by an energy transfer to Pr 3+ , and consequently generates the afterglow. The experimental results also show that the afterglow characteristics of Pr 3+ are prolonged by incorporating Pr 3+ in the CaWO 4 matrix.
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高影响因子
CERAMICS INTERNATIONAL
·
2019
·
IF 3.45
Jinming Shi,Yue Chang,Yuanshun Tang,Xuanbo Wang,Wenqing Yao,Zhenxing Yue,Badar Minhas,Jiangli Cao
Abstarct Ferrous titanate (FeTiO 3 ) nanoparticles with hexagonal disc structure were prepared by a simple hydrothermal method . The VSM results show that ferromagnetic-paramagnetic transition of FeTiO 3 caused by high temperature hydrogenation. The morphology and lattice structure of FeTiO 3 was characterized in detail by XRD , Raman, SEM and TEM . It was found that the magnetic transition of FeTiO 3 nanoparticles after hydrogenation could be attributed to the change of lattice structure and surface effect. Further research by XPS found that the high temperature hydrogenation treatment could cause the change of elemental valence states and oxygen vacancies concentration for FeTiO 3 . The weakening of double exchange interaction (Fe 2+ -O-Fe 3+ ) and the reduction of uncompensated surface spin were the main cause of the ferromagnetic-paramagnetic transition. This experiment would provide a new idea for regulating the magnetic property of materials.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Rongsheng Li,Xiaochen Ma,Yuanyuan Han,Tingting Yang,Rui Li,Dong Wang,Luqi Huang,Xueli Zhang,Zhubo Dai
Steroid hormones comprise hundreds of clinically used drugs, and their de novo biosynthesis from simple bio-based carbon sources holds great potential for advancing novel green industries. As a vital class of steroid hormones, progestogens are widely used to treat menstrual disorders, breast cancer, and pregnancy-related conditions. Here, we identified a novel steroid dehydrogenase from Marsdenia tenacissima , Mt CYP82BQ1, which catalyzes Δ 6 -dehydrogenation of progesterone, leading to the synthesis of 6-dehydroprogesterone, a non-natural progestogen with therapeutic potential. Through homology modeling and site-directed mutagenesis experiments, the key residues governing the catalytic activity of Mt CYP82BQ1 were identified. In order to construct an efficient cell factory for the production of 6-dehydroprogesterone. Initially, the best performing P450 sterol side chain cleavage enzyme (P450scc) Cc CYP87N1 and 3 β -hydroxysteroid dehydrogenase/Δ 5 -Δ 4 ketosteroid isomerase (3 β HSD) Cc HSD6 from Cynanchum chinense were screened for progesterone synthesis, a key precursor in the synthesis of 6-dehydroprogesterone. Subsequently, semi-rational design of Cc CYP87N1 obtained the Cc CYP87N1 F282W mutant with 74.4 % increased activity, which further improved progesterone titer by 14.0 %. Through further metabolic regulation and fermentation optimization, the progesterone titer reached 1.34 g/L in a 5 L bioreactor. Furthermore, advantageous Mt CYP82BQ1 variants were employed to construct the 6-dehydroprogesterone-producing strains. The optimization of heme metabolism boosted the 6-dehydroprogesterone titer by 107.3 %, achieving 26.1 mg/L. Finally, homologous enzymes of Ps CYP82BQ2 from Periploca sepium , exhibiting a high substrate conversion rate of 90.4 %, were screened and utilized to improve the 6-dehydroprogesterone production. The 6-dehydroprogesterone titer was further increased by 28.9 %, and a final titer of 0.72 g/L was achieved through fed-batch fermentation. This work provides a sustainable platform for the industrial-scale production of progesterone derivatives.
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高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Longsheng Chen,Wei Xu,Rong Shao,Xianfeng Du
The profile and bioactivity of phytochemicals in Araiostegia yunnanensis ( Christ ) Cop were investigated. The total flavonoids content in A. yunnanensis is about 84.90 mg/g. By means of HPLC–DAD–ESI-MS, the main flavonoids in A. yunnanensis were tentatively identified as myricetin 3- O -rhamnosylglucoside, eriodictyol 7- O -rutinoside, quercetin 3- O -rutinoside, luteolin-7- O -apiosylglucoside, quercetin 3- O -rhamnosylgalactoside, and luteolin 7- O -glucoside. The extract (0.268 mg/ml total flavonoids) from A. yunnanensis showed very strong superoxide anion radical scavenging potential and reducing power, which are higher than those of rutin (0.25 mg/ml). The extract (0.268 mg/ml total flavonoids) from A. yunnanensis exhibited similar DPPH scavenging activity with rutin (0.25 mg/ml). However, rutin (0.25 mg/ml) showed a significantly higher ABTS radical scavenging effect than that of the extract (0.268 mg/ml total flavonoids) from A. yunnanensis. The methanol extract from A. yunnanensis showed obviously cytotoxic effects on A549 cells and it had no effect against acetylcholinesterase.
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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2025
·
IF 4.3
Yunshuo Chen,Yachan Gong,Dan Li,Weiwei Feng,Yao Chen,Ting Zhao,Liuqing Yang,Guanghua Mao,Xiangyang Wu
As a typical neonicotinoid insecticide, dinotefuran (DIN) has immunotoxicity, but its immunotoxicity effects on aquatic organisms and its molecular mechanisms are not known. In this study, zebrafish embryos were used as model to reveal its immunotoxicity and its mechanism from the perspective of inflammatory response. Zebrafish larvae were exposed to DIN at environmentally relevant concentrations (0, 2, 200, and 2000 mg/L) for 120 h, followed by comprehensive analyses of immune cell populations, immune marker activities, oxidative stress levels, and gene expression profiles. The results showed that the number of neutrophils and macrophages, two types of innate immune cells, was significantly reduced, and the activities of the immune markers, lysozyme (LYS), immunoglobulin M (IgM), and complement protein C3, were significantly inhibited. The level of oxidative stress in zebrafish larvae was significantly elevated and antioxidant enzyme activities were markedly inhibited in a dose-dependent manner after exposure to DIN. In this case, pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β were also abnormally expressed, and the expression levels of the genes related to the NF-κB pathway and the JNK-STAT pathway, tlr4a, myd88, nf-κb p65, jak1, jak2 , and stat3 , were elevated. The expression levels of genes related to the antioxidant signaling pathway Nrf2-keap1 signaling pathway were suppressed. Taken together, our results suggest that DIN exposure causes damage to the immune system of zebrafish embryos-larvae, generating oxidative stress and inflammatory responses that lead to immunotoxicity.
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高影响因子
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