Nature Communications
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2024
·Vol. 15
·期 1
·1-19
·
IF 14.7
Liu Tao,Chai Shuang,Li Mingyang,Chen Xu,Xie Yutao,Zhao Zehui,Xie Jingjing,Yu Yunpeng,Gao Feng,Zhu Feng,Yang Lihua
Infection by Helicobacter pylori , a prevalent global pathogen, currently requires antibiotic-based treatments, which often lead to antimicrobial resistance and gut microbiota dysbiosis. Here, we develop a non-antibiotic approach using sonodynamic therapy mediated by a lecithin bilayer-coated poly(lactic-co-glycolic) nanoparticle preloaded with verteporfin, Ver-PLGA@Lecithin, in conjunction with localized ultrasound exposure of a dosage permissible for ultrasound medical devices. This study reveals dual functionality of Ver-PLGA@Lecithin. It effectively neutralizes vacuolating cytotoxin A, a key virulence factor secreted by H. pylori , even in the absence of ultrasound. When coupled with ultrasound exposure, it inactivates H. pylori by generating reactive oxygen species, offering a potential solution to overcome antimicrobial resistance. In female mouse models bearing H. pylori infection, this sonodynamic therapy performs comparably to the standard triple therapy in reducing gastric infection. Significantly, unlike the antibiotic treatments, the sonodynamic therapy does not negatively disrupt gut microbiota, with the only major impact being upregulation of Lactobacillus , which is a bacterium widely used in yogurt products and probiotics. This study presents a promising alternative to the current antibiotic-based therapies for H. pylori infection, offering a reduced risk of antimicrobial resistance and minimal disturbance to the gut microbiota.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-13
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IF 5.3
Wang Yutong,Liang Xiao,Yang Yaling
Se-WCDs-Au-Janus Ag NPs with weak oxidase activity were synthesized using Se-W carbon dots (Se-WCDs) as stabilizers and reducing agents. These Janus nanoparticles (Janus NPs) effectively catalyze the reduction of mercury ions (Hg 2+ ) to metallic mercury (Hg 0 ), generating Au–Ag-Hg mercury alloys (Au–Ag@HgNPs). The aggregated Au–Ag@HgNPs on the nanoparticles improve the SERS activity of Se-WCDs-Au-Janus Ag NPs through a synergistic effect of electromagnetic enhancement and chemical enhancement. Interestingly, adding FB1 turns off the Raman signal by inhibiting the aggregation of Au–Ag@HgNPs. Based on these findings, a SERS “on–off” sensor utilizing the Se-WCDs-Au-Janus Ag NPs as the probe was successfully developed to determine fumonisin B1 (FB1) and Hg 2+ in grains. The detection limits were as low as 0.005 μg·L −1 for Hg 2+ and 0.006 μg·L −1 for FB1. Graphical abstract
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JOURNAL OF POLYMER RESEARCH
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2010
·Vol. 18
·期 6
·1261-1268
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IF 1.364
Zhang Gang,Huang Guang-shun,Wang Xiao-jun,Long Sheng-ru,Yang Jie
Two kinds of aromatic dibenzoyl chloride monomer containing thioether unit 4,4′-Bis(4-chloroformylphenylthio)benzophenone (BP-DC) and 4,4′-thiodibenzoyl chloride (T-DC) were synthesized with two steps, which was reacted with diamine monomer containing thioether and amide unit to prepare a new kind of polyamide containing high quantity thio-ether group. The intrinsic viscosity of the polyamides was 0.76–0.90 dl/g obtained with optimum synthesis conditions. The polymers were found to have good optical properties: the optical transmittance of the aromatic polyamide film at 450 nm is higher than 80%, meantime the high quantity thio-ether unit provided the polymer with a high refractive index ranging from 1.691 to 1.696 and low birefringence of 0.007–0.008. At the same time the polymers had excellent thermal performance with glass transition temperature (T g ) of 226 °C–278 °C, initial degradation temperature (T d ) of 427 °C–439 °C. They showed improved solubility in polar aprotic solvents.
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Parasites & Vectors
·
2024
·Vol. 17
·期 1
·1-17
·
IF 3
Chen Huifang,Li Juan,Pan Xiaoting,Hu Zhichao,Cai Jianfeng,Xia Zijie,Qi Nanshan,Liao Shenquan,Spritzer Zachary,Bai Yinshan,Sun Mingfei
Background The gastrointestinal epithelium plays an important role in directing recognition by the immune system, and epithelial cells provide the host's front line of defense against microorganisms. However, it is difficult to cultivate avian intestinal epithelial cells in vitro for lengthy periods, and the lack of available cell lines limits the research on avian intestinal diseases and nutritional regulation. Chicken coccidiosis is a serious intestinal disease that causes significant economic losses in the poultry industry. In vitro, some cell line models are beneficial for the development of Eimeria species; however, only partial reproduction can be achieved. Therefore, we sought to develop a new model with both the natural host and epithelial cell phenotypes.Methods In this study, we use the SV40 large T antigen (SV40T) gene to generate an immortalized cell line. Single-cell screening technology was used to sort positive cell clusters with epithelial characteristics for passage. Polymerase chain reaction (PCR) identification, immunofluorescence detection, and bulk RNA sequencing analysis and validation were used to check the expression of epithelial cell markers and characterize the avian intestinal epithelial cell line (AIEC). AIECs were infected with sporozoites, and their ability to support the in vitro endogenous development of Eimeria tenella was assessed.Results This novel AIEC consistently expressed intestinal epithelial markers. Transcriptome assays revealed the upregulation of genes associated with proliferation and downregulation of genes associated with apoptosis. We sought to compare E. tenella infection between an existing fibroblast cell line (DF-1) and several passages of AIEC and found that the invasion efficiency was significantly increased relative to that of chicken fibroblast cell lines.Conclusion sAn AIEC will serve as a better in vitro research model, especially in the study of Eimeria species development and the mechanisms of parasite–host interactions. Using AIEC helps us understand the involvement of intestinal epithelial cells in the digestive tract and the immune defense of the chickens, which will contribute to the epithelial innate defense against microbial infection in the gastrointestinal tract.Graphical Abstract
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JOURNAL OF NANOBIOTECHNOLOGY
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2024
·Vol. 22
·期 1
·1-26
·
IF 10.6
Hu Yu,Cui Jingwen,Sun Junpeng,Liu Xiaobang,Gao Shuang,Mei Xifan,Wu Chao,Tian He
Alzheimer’s disease (AD) is a common neurodegenerative disease characterized by progressive cognitive and physical impairment. Neuroinflammation is related to AD, and the misfolding and aggregation of amyloid protein in the brain creates an inflammatory microenvironment. Microglia are the predominant contributors to neuroinflammation, and abnormal activation of microglia induces the release of a large amount of inflammatory factors, promotes neuronal apoptosis, and leads to cognitive impairment. In this study, we used microglial membranes containing caffeic acid-coupled carbon quantum dots to prepare a novel biomimetic nanocapsule (CDs-CA-MGs) for the treatment of AD. The application of CDs-CA-MGs via nasal administration can bypass the blood‒brain barrier (BBB) and directly target the site of inflammation. After treatment with CDs-CA-MGs, AD mice showed reduced inflammation in the brain, decreased neuronal apoptosis, and significantly improved learning and memory abilities. In addition, CDs-CA-MGs affect inflammation-related JAK-STAT and Toll-like receptor signaling pathways in AD mice. CDs-CA-MGs significantly downregulated interleukins (IL-1β and IL-6) and tumor necrosis factor (TNF-α). This finding suggested that CDs-CA-MGs may improve cognitive impairment by modulating inflammatory responses. In conclusion, the use of CDs-CA-MGs provides a possible therapeutic strategy for the treatment of AD.Graphical abstract
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Cell Death & Disease
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2024
·Vol. 15
·期 1
·1-15
·
IF 8.1
Li Jingzhi,Guo Ming,Chen Lin,Chen Zhuchu,Fu Ying,Chen Yongheng
Nasopharyngeal carcinoma (NPC) is a malignant tumor that is highly prevalent in Southeast Asia, especially in South China. The pathogenesis of NPC is complex, and genetic alterations of tumor suppressors and proto-oncogenes play important roles in NPC carcinogenesis. p53 is unexpectedly highly expressed in NPC and possesses an uncommon mutation of R280T, which is different from a high frequency of hotspot mutations or low expression in other tumors. However, the mechanism of p53 loss of function and its correlation with R280T in NPC are still unclear. In this study, p53 amyloid aggregates were found to be widespread in NPC and can be mainly induced by the R280T mutation. Aggregated p53-R280T impeded its entry into the nucleus and was unable to initiate the transcription of downstream target genes, resulting in decreased NPC cell cycle arrest and apoptosis. In addition, NPC cells with p53-R280T amyloid aggregates also contributed aggressively to tumor growth in vivo. Transcriptome analysis suggested that p53 amyloid aggregation dysregulated major signaling pathways associated with the cell cycle, proliferation, apoptosis, and unfolded protein response (UPR). Further studies revealed that Hsp90, as a key molecular chaperone in p53 folding, was upregulated in NPC cells with p53-R280T aggregation, and the upregulated Hsp90 facilitated p53 aggregation in turn, forming positive feedback. Therefore, Hsp90 inhibitors could dissociate p53-R280T aggregation and restore the suppressor function of p53 in vitro and in vivo. In conclusion, our study demonstrated that p53-R280T may misfold to form aggregates with the help of Hsp90, resulting in the inability of sequestered p53 to initiate the transcription of downstream target genes. These results revealed a new mechanism for the loss of p53 function in NPC and provided novel mechanistic insight into NPC pathogenesis.
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Advanced Fiber Materials
·
2024
·
IF 17.2
Tong Xiaoling,Hua Tianjiao,Xu Miaoyi,Yang Dongzi,Xiao Gang,Li Shuo,Cao Xiaohui,Shao Yuanlong
The rapid advancement of personalized healthcare brings forth a myriad of self-powered integrated sweat fabric systems. However, challenges such as alkaline by-products, low open-circuit voltage and output power have made them unsuitable for the continuously powering biosensors. Here, we have designed a sweat-activated polyaniline/single-wall carbon nanotube||Zinc (PANI/SWCNTs||Zn) battery fabric featuring multiple redox states. This innovative battery achieves a high open-circuit voltage of 1.2 V within 1.0 s and boasts an impressive power density of 2.5 mW cm −2 due to the rapid solid–liquid two-phase electronic/ionic transfer interface. In-depth characterization reveals that the discharge mechanism involves the reduction of emeraldine salt to leucoemeraldine without oxygen reduction. By integrating this system seamlessly, the sweat-activated batteries can directly power a patterned light-emitting diode and a multiplexed sweat biosensor, while wirelessly transmitting data to a user interface via Bluetooth. This strategic design offers safety warnings and continuous real-time health monitoring for night walking or running. This work paves the way for the development of an efficient and sustainable energy-autonomous electronic fabric system tailored for individual health monitoring. Graphical Abstract Highly power-density sweat-activated PANI/SWCNTs||Zn fiber battery has been fabricated by rapid reduction of emeraldine salt to leucoemeraldine. Through seamless system integration, the thus-fabricated sweat-activated battery pack can power a multiplexed sweat biosensor, demonstrating the feasibility of a sustainable energy-autonomous electronic fabric system for continuous individual health monitoring.
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Chinese Medicine
·
2024
·
IF 5.3
Dandan Zhang,Junyu Zhang,Simian Chen,Hairong Zhang,Yuexin Yang,Shan Jiang,Yun Hong,Mingshe Zhu,Qiang Xie,Caisheng Wu
Background Deciphering the in vivo processes of traditional Chinese medicine (TCM) is crucial for identifying new pharmacodynamic substances and new drugs. Due to the complexity and diversity of components, investigating the exposure, metabolism, and disposition remains a major challenge in TCM research. In recent years, a number of non-targeted smart mass-spectrometry (MS) techniques, such as precise-and-thorough background-subtraction (PATBS) and metabolomics, have realized the intelligent identification of in vivo components of TCM. However, the metabolites characterization still largely relies on manual identification in combination with online databases. Results We developed a scoring approach based on the structural similarity and minimal mass defect variations between metabolites and prototypes. The current method integrates three dimensions of mass spectral data including m/z , mass defect of MS1 and MS2, and the similarity of MS2 fragments, which was sequentially analyzed by a R-based mass dataset relevance bridging (MDRB) data post-processing technique. The MDRB technology constructed a component relationship network for TCM, significantly improving metabolite identification efficiency and facilitating the mapping of translational metabolic pathways. By combining MDRB with PATBS through this non-targeted identification technology, we developed a comprehensive strategy for identification, characterization and bridging analysis of TCM metabolite in vivo. As a proof of concept, we adopted the proposed strategy to investigate the process of exposure, metabolism, and disposition of Semen Armeniacae Amarum (CKXR) in mice. Significance The currently proposed analytical approach is universally applicable and demonstrates its effectiveness in analyzing complex components of TCMs in vitro and in vivo. Furthermore, it enables the correlation of in vitro and in vivo data, providing insights into the metabolic transformations among components sharing the same parent nucleus structure. Finally, the developed MDRB platform is publicly available for ( https://github.com/933ZhangDD/MDRB ) for accelerating TCM research for the scientific community. Graphical
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Wang Xin,Xia Neng,Pan Chengfeng,Zhao Jinsheng,Hao Bo,Su Lin,Jin Dongdong,Xu Qingsong,Liu Xurui,Hou Xingyu,Zhang Li
Water surface jumping motions of semi-aquatic insects are primarily rely on surface tension-dominated jumping mechanism to achieve impressive jumping performance. However, this mechanism faces an inherent physical constraint: the propulsion force must remain below the threshold required to break the water surface, limiting efficient momentum acquisition. Herein, we present a water surface jumping strategy that addresses the limitations of surface tension-dominated mechanism. Our approach allows the engineered jumper to achieve a record-breaking jumping height of 18 body lengths (63 cm) and take-off velocity of 100.6 body length/s (3.52 m/s). This strategy is built on three key design principles: (I) superhydrophobic body for floating on water surface, (II) light-weight, high-power actuation module capable of providing significant propulsion force within an ultrashort time, (III) well-engineered momentum transmission system for efficient kinetic energy transfer. The developed soft jumper based on these design principles advances the development of water environment related robotics.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-10
·
IF 5.3
Yu Pengfei,Li Meixin,Wang Shun,Li Shanshan,Cui Jiaqi,Yang Jun,Liu Shuang,Kong Ling,Chen Zhiwei
Fumonisin B 1 (FB 1 ) is a mycotoxin mainly found in corn, peanuts, and wheat crops, which affects human health. Based on bismuth sulfide/bismuth oxychloride (Bi 2 S 3 /BiOCl) composite material, silver sulfide (Ag 2 S) was grown in situ as a quantum dot sensitization signal, and a photoelectrochemical (PEC) aptasensor was designed by layer upon layer modification to detect FB 1 . Bi 2 S 3 /BiOCl has a wide range of visible light absorption, stable chemical properties, and a simple synthesis method. In the construction process, L-ascorbic acid (AA) is selected to provide electrons and inhibit photogenerated electron-hole (e - /h + ) recombination. Under the optimal experimental conditions, the detection range of the fabricated PEC aptasensor was 0.001 ~ 100 ng/mL, and the detection limit was 0.016 pg/mL. The prepared PEC aptasensor has high sensitivity, stability, and reproducibility. The combination of aptamer and PEC sensor provides a novel method for the application of PEC sensor in mycotoxin detection. Graphical
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KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 3.2
Mao Xiqiang,Zou Sujing,Lu Xue,Li Shaoyi,Wu Lei,Li Jun,Yang Jian,Fan Ximei
Photocatalysis technology has garnered significant attentions for addressing water pollution issues, however, conventional photocatalysts face great challenges such as poor recoverability, low light utilization rates, and severe caking that hinder their widespread use. This study presents the development of floating Bi 2 O 3 /TiO 2 /HGM (BTH) photocatalysts utilizing hollow glass microspheres (HGM) as a supporting material. The optimized heterojunction of BTH-0.3 achieves an 83.9% removal rate of tetracycline hydrochloride within 60 min irradiation under simulated sunlight, surpassing the 77.3% achieved by TH-2 loaded with only TiO 2 and the 62% achieved by pure Bi 2 O 3 . Furthermore, BTH-0.3 exhibits outstanding photocatalytic performance across varying solution pH levels and diverse water matrices. Moreover, consistent tetracycline hydrochloride removal rates were observed over four consecutive degradation cycles, demonstrating its excellent reusability. This research offers a promising approach with practical potential for addressing water pollution challenges via photocatalytic technology.
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JOURNAL OF FLUORESCENCE
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2024
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IF 2.6
Li Qianwen,Yin Yu,Wang Wenjuan,Liu Bin,Tong Wei,Zhang Xu,Liu Jinquan,Yang Shengyuan
Herein, a visual and luminescent dual-mode (colorimetric and fluorometric) method for the detection of P-phenylenediamine (PPD) in hair dye was successfully established based on cerium-nitrogen co-doped carbon dots (Ce, N-CDs) that displayed remarkable luminescence and peroxidase activity. Ce, N-CDs catalyzed H 2 O 2 to produce superoxide anion, which then oxidized the colorless 3,3,5,5-tetramethylbenzidine (TMB) into blue oxidized TMB (oxTMB), capable of quenching the fluorescence through fluorescence resonance energy transfer (FRET) between Ce, N-CDs and oxTMB. The reducing properties of PPD could reduce oxTMB back to TMB, leading to a decrease in the absorption intensity of oxTMB and a fluorescence recovery of Ce, N-CDs. As a result, the quantitative detection of PPD could be achieved by measuring the absorption values of oxTMB and the fluorescence signal of Ce, N-CDs. The detection limits for PPD were calculated as 0.36 µM and 0.10 µM for colorimetry and fluorimetry, respectively. Furthermore, smartphone application (ColorPicker) capable of measuring the RGB value of the color was utilized in the detection system, facilitating on-site quantitative detection. This approach effectively shortens the detection time and simplifies the operation, offering a powerful and convenient tool for real-time monitoring of PPD.
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