MICROCHIMICA ACTA
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2020
·Vol. 187
·期 6
·1-8
·
IF 6.232
Cai Xiaolei,Lv Fuhui,Lai Guosong,Fu Li,Lin Cheng-Te,Yu Aimin
A magnetic bead (MB)-based sandwich biorecognition reactions is combined with a gold nanoprobe-induced homogenous synthesis of molybdophosphate to develop a novel bioassay method for the electrochemical detection of the tumor biomarker of carcinoembryonic antigen (CEA). The nanoprobe is prepared through the specific loading of numerous alkaline phosphatase (ALP)-functionalized gold nanoparticles (Au NPs) on a double-stranded DNA (dsDNA) produced by the CEA aptamer-triggered hybridization chain reaction (HCR). Both the large amounts of PO 4 3− produced by the ALP catalytic hydrolysis of pyrophosphate and the phosphate backbones of dsDNA can react with the added MoO 4 2− to generate electroactive molybdophosphates. So, the gold nanoprobe was used for signal tracing of the sandwich bioassay of CEA at a constructed antibody-functionalized MB platform. The sensitive electrochemical measurement of molybdophosphate produced from the quantitatively captured nanoprobes at a carbon nanotube-modified electrode (measured at about 0.12 V vs. Ag/AgCl, 3 M KCl) enabled the convenient signal transduction of the method. Due to the dually enhanced synthesis of molybdophosphate by the HCR and multi-enzyme Au NP nanotags, this method shows a wide linear range from 0.05 pg mL −1 to 10 ng mL −1 along with a low detection limit of 0.027 pg mL −1 . In addition, the MB-based biorecognition reaction and the homogeneous synthesis of molybdophosphate are much convenient in manipulations. These excellent performances decide the extensive application potentials of the method.
A magnetic bead-based bioassay method was simply developed for the electrochemical detection of carcinoembryonic antigen. The dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction (HCR) and enzyme nanotags and the sensitive electrochemical measurement of molybdophosphate at a carbon nanotube (CNT)-electrode enable ultrasensitive signal transduction of the method.
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高影响因子
International Journal of Environmental Science and Technology
·
2020
·Vol. 17
·期 5
·3055-3064
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IF 2.54
Liu S.-S.,Luo D.-M.,Yang X.,Tong C.-X.,Tao L.-G.,Ding H.-P.,Zhang J.
A series of graphitic carbon nitrides (g-C 3 N 4 ) were prepared using different precursors; these g-C 3 N 4 -supported CoOx catalysts exhibited different catalytic performances in toluene catalytic combustion reaction. The structure of samples was detected by XRD, TEM, SEM, XPS, H 2 -TPR, nitrogen adsorption and desorption technique, to build up the structure–performance relationship and uncover the key property of g-C 3 N 4 that mostly influences the catalytic performance of CoOx/g-C 3 N 4 . CoOx with average particle size of 12.7 nm supported on g-C 3 N 4 prepared by dicyandiamide showed better catalytic performance than on other supports, 100% toluene conversion could be achieved at ~ 285 °C, characterization results showed that Co was directly connected with N, and the integrity of g-C 3 N 4 sheet affects the dispersion of CoOx; the content of sp 2 -N (within C=N–C structure) determines the interaction between g-C 3 N 4 and CoOx; these two factors together affect the performance of CoOx/g-C 3 N 4 catalysts in the catalytic combustion of toluene.
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SN Applied Sciences
·
2020
·Vol. 2
·期 3
·1-10
·
IF 0
Jia Shuyue,Wang Qidong,Peng Jing,Gao Jingru,Tang Dongyan
ZnO nanomaterials have been widely studied in medical and biological fields owing to their better biocompatibility, pH-responsibility and higher photocatalytic ability as drug carriers and disease therapy agent. However, the unstability of ZnO nanomaterials within aqueous solution restricts the applications of it to some extent. In this work, ZnO nanofibers with relative higher porosity were fabricated by electrospinning to improve the load capacity as carriers, and then were modified by hydrophobic poly(methyl methacrylate) (PMMA) through graft polymerization on the surface to improve their stability in aqueous solution. Observations on the morphologies of the nanofibers by SEM showed that the surface modification of PMMA well enhanced the water-stability of ZnO nanofibers. Moreover, the photocatalytic degradation of Rhodamine B was investigated, and pH sensitive release behaviors were studied in details under different pH values, by taking Nifedipine as drug model. The results indicated that PMMA modified ZnO nanofibers showed obvious photocatalytic performances and maintained relative excellent pH-response properties. These findings all suggested that the modified ZnO nanofibers might be one kind of excellent material as medical carrier or therapy agents.
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Silicon
·
2020
·Vol. 12
·期 12
·3005-3013
·
IF 1.499
Pan Zhaoqun,Zeng Kelin,Huang Bingsheng,Zhu Liqiang
The oligosiloxane resins were synthesized through hydrolytic sol-gel reaction and remained many hydroxyl groups, which did great harm to the curing process and resulted in poor performance of the cured products. In previous works, epoxy-modified silicone resins were synthesized by dealcoholization, a reaction between 3-glycidoxypropylmethyldimethoxysilane and terminal hydroxyl groups in phenyl silicone resins. Although this method eliminated the hydroxyl groups, it caused a large loss of vinyl groups inevitably and a poor stability of cured products. In this study, methyltrimethoxysilane (MTMS) was used to eliminate hydroxyl groups containing in vinyl methyl phenyl silicone resins, which were synthesized through hydrolytic sol-gel reaction. Most of residual hydroxyl groups were deleted effectively and the great loss of vinyl groups were avoided in the dealcoholization reaction. Then, the methyl phenyl silicone materials were fabricated by hydrosilylation reaction between the synthesized vinyl methyl phenyl silicone resins and hydrogen-containing ones. The cured silicone materials showed excellent optical performance (~ 89.1% at 450 nm) and good adhesion performance. In addition, due to less vinyl loss in the vinyl methyl phenyl silicone resins, the cured methyl phenyl silicone materials exhibited higher cross-linking density, better thermal resistance (5% mass loss at 435 °C) and better mechanical properties (50 shore D) compared with the epoxy-modified phenyl silicone materials. The lumen depreciation (working 168 h at 50 mA) and reflow soldering tests further demonstrated the methyl phenyl silicone materials possessed good thermal stability. These results indicated that the methyl phenyl silicone materials could be used as a LED encapsulant with a good performance.
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FIBERS AND POLYMERS
·
2020
·Vol. 21
·期 1
·119-126
·
IF 1.797
Xu Wenlong,Yang Xue,Xin Binjie
Polyacrylonitrile (PAN)/cellulose nanofibril (CNF) nanofiber membranes were successfully prepared by an electrospinning method, in which the amount of CNF kept 1 wt%. The effects of CNF sizes on the morphology and properly of spinning solutions and PAN electrospun nanofiber membrane were systematically investigated by rheological property test, conductivity test, SEM, TG, DSC, contact angle test and mechanical test. The results showed that the viscosity and conductivity of the spinning solution was improved with the increasing of CNF sizes, as the formation of the strong polar interactions of nitrile groups and the hydroxyl groups between PAN and CNF. All the morphology of the PAN/CNF nanofiber membrane exhibited a uniform diameter with no visible beads or beads to the string structure, and the average diameter of the PAN/CNF nanofiber was decreased with the increasing of the CNF size. Moreover, the addition of CNF could improve the thermal stability, mechanical properly, and hydrophilicity of the PAN membrane. And the PAN/CNF-10 nanofiber membrane exhibited the optimal properties.
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COLLOID AND POLYMER SCIENCE
·
2020
·Vol. 298
·期 9
·1187-1195
·
IF 1.536
Xu Pingping,Tan Yun,Wang Xionglei,Xu Huaxiu,Wang Di,Yang Yang,An Wenli,Xu Shimei
We prepared two types of multidimensional gradient hydrogels by simple electrophoresis using spherical graphite electrode. To explore their application in controlled drug release, rhodamine B (RB) and sodium salicylate (SS) were used as oppositely charged drug models respectively to in situ loading into the hydrogels by electrostatic interaction with the charged components accordingly. In both cases, the model drugs and the charged components of the hydrogels were gradiently distributed with the drug concentration increased from the gel surface to the gel core. For the crosslinking gradient hydrogel prepared at 2 V, the network size near the gel surface is about 3 times that of the gel core, and the release rate is 0.85 times of that of the non-gradient hydrogel. For the polymer chain gradient hydrogel prepared at 2.5 V, the relative content of polymer chain near the core of the gel is 2.5 times that of the gel surface, and the release rate is 0.71 times of the non-gradient hydrogel.
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Science China-Technological Sciences
·
2020
·Vol. 63
·期 11
·2449-2463
·
IF 2.302
Gao Shang,Qu LinLin,Zhu ChenHui,Ouyang PingKai,Fan DaiDi
In situ injectable hydrogels, which have great potential in tissue engineering, are characterized by simple preparation, minimal invasiveness and adaptation to complex shapes. However, injectable hydrogels have higher requirements for biocompatibility and safety due to their use in vivo implantation. Therefore, in this study, a human-like collagen (HLC)-based in situ gel-forming injectable HLC-HPA hydrogel was synthesized by combining the amino group of HLC with the carboxyl group of HPA activated using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), followed by crosslinking by horseradish peroxidase (HRP) and H 2 O 2 , and used as scaffold material for tissue engineering. The hydrogel stiffness, gel time and biodegradation rate could be easily and independently adjusted by varying the H 2 O 2 and HRP concentrations. Scanning electron microscope (SEM) clarified the homogeneous porous and interconnected internal structures of the hydrogel. In vitro cell viability and in vivo degradation experiments confirmed that the HLC-HPA hydrogel had good biodegradability and excellent biocompatibility. Interestingly, in cultured macrophages, the HLC-HPA hydrogel showed anti-inflammatory activity by reducing the amount of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) and increasing the secretion of the anti-inflammatory cytokine interleukin-10 (IL-10) induced by lipopolysaccharide (LPS). Meanwhile, in animal experiments, HLC-HPA hydrogels exhibit excellent biocompatibility and have properties of hemostasis and reduction of inflammatory response. Therefore, the HLC-HPA hydrogel prepared in this study has great potential for development for use in the biomedical field.
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高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 8
·1549-1555
·
IF 2.667
Qu Yaobaixue,Yang Zhao-Guang,Li Haipu,Yao Jie,Qiu Bo
An ultrasound-assisted enzymatic extraction followed by a diluted acid washing was introduced into the multi-element analysis of rice samples. The recoveries of the elements including As, Cd, Mn, and Zn were evaluated by inductively coupled plasma mass spectrometry. The experimental parameters including enzyme type, enzyme dosage, ultrasound power, and duration of ultrasonication were investigated. The optimized condition was pancreatin with the dosage of 6.75 mg enzyme to 30-mg rice and ultrasonication for 5 min with the power of 800 W. The whole extraction procedure can be accomplished in 20 min. Three certified reference rice samples (GBW (E) 100348, GBW10043, and GBW10044) were used for method verification. The recoveries of As, Cd, Mn, and Zn ranged from 82.2 to 97.3%. The relative standard deviations of the four elements were all lower than 10%. Finally, the present method was successfully applied in the multi-element analysis of real samples.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 12
·9543-9549
·
IF 2.22
Liu Wanying,Zhu Yabo,Xu Xiang,Wang Shasha,Zhang Xinxi
The wet ball milling (WBM) method is, for the first time, used for the exfoliation of bulk black phosphorus (BP) to obtain few-layer black phosphorus (FBP). Before that, the rational solid–liquid ratio of raw material is determined to be 1:1 (mg/mL) for the popular ultrasound liquid exfoliation (ULE). Obviously, the high proportion of organic solvent used in ULE will lead to serious environmental pollution. Compared with ULE, the solid/liquid ratio for WBM exfoliation is 100:1 (mg/mL) showing its advantage of less demand for organic solvent. In addition, different types of organic solvents are used to determine the proper organic solvent for WBM exfoliation. Ultraviolet–visible–near-infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and laser particle size analyzer are used to comprehensively characterize the layer numbers, morphology size, and phase structure of FBP yielded by WBM exfoliation. The results show that dimethyl sulfoxide (DMSO) is the suitable organic solvent for BP exfoliation; FBP flakes with 3–5 layers and micron size are obtained by WBM exfoliation.
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Chinese Medicine
·
2020
·Vol. 15
·期 1
·1-18
·
IF 2.96
Qin Zifei,Jia Mengmeng,Yang Jing,Xing Han,Yin Zhao,Yao Zhihong,Zhang Xiaojian,Yao Xinsheng
Background Kang-Ai injection is widely used as an adjuvant therapy drug for many cancers, leukopenia, and chronic hepatitis B. Circulating alkaloids and saponins are believed to be responsible for therapeutic effects. However, their pharmacokinetics (PK) and excretion in vivo and the risk of drug–drug interactions (DDI) through inhibiting human cytochrome P450 (CYP) and UDP-glucuronosyltransferase (UGT) enzymes remain unclear.Methods PK and excretion of circulating compounds were investigated in rats using a validated ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC–MS) method. Further, the inhibitory effects of nine major compounds against eleven CYP and UGT isozymes were assayed using well-accepted specific substrate for each enzyme.Results After dosing, 9 alkaloids were found with Cmax and t1/2 values of 0.17–422.70 μmol/L and 1.78–4.33 h, respectively. Additionally, 28 saponins exhibited considerable systemic exposure with t1/2 values of 0.63–7.22 h, whereas other trace saponins could be negligible or undetected. Besides, over 90% of alkaloids were excreted through hepatobiliary and renal excretion. Likewise, astragalosides and protopanaxatriol (PPT) type ginsenosides also involved in hepatobiliary and/or renal excretion. Protopanaxadiol (PPD) type ginsenosides were mainly excreted to urine. Furthermore, PPD-type ginsenosides were extensively bound (fu-plasma approximately 1%), whereas astragalosides and PPT-type ginsenosides displayed fu-plasma values of 12.35% and 60.23–87.36%, respectively. Moreover, matrine, oxymatrine, astragaloside IV, ginsenoside Rg1, ginsenoside Re, ginsenoside Rd, ginsenoside Rc, and ginsenoside Rb1 exhibited no inhibition or weak inhibition against several common CYP and UGT enzymes IC50 values between 8.81 and 92.21 μM. Through kinetic modeling, their inhibition mechanisms towards those CYP and UGT isozymes were explored with obtained Ki values. In vitro-in vivo extrapolation showed the inhibition of systemic clearance for CYP or UGT substrates seemed impossible due to [I]/Ki no more than 0.1.Conclusion sWe summarized the PK behaviors, excretion characteristics and protein binding rates of circulating alkaloids, astragalosides and ginsenosides after intravenous Kang-Ai injection. Furthermore, weak inhibition or no inhibition towards these CYP and UGT activities could not trigger harmful DDI when Kang-Ai injection is co-administered with clinical drugs primarily cleared by these CYP or UGT isozymes.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 23
·5627-5635
·
IF 3.637
Tan Hongxia,Guo Ting,Zhou Hongyuan,Dai Hongjie,Yu Yong,Zhu Hankun,Wang Hongxia,Fu Yu,Zhang Yuhao,Ma Liang
Zearalenone (ZEN) is a type of estrogenic mycotoxin commonly occurring in cereals. The aim of this study was to design a simple, rapid, inexpensive and ultrasensitive fluorescence assay for the determination of ZEN. Here, amino-modified mesoporous silica nanoparticles (MSNs-NH 2 ) were synthesized to be the positive charge-rich reactor. A 6-carboxy-fluorescein-labeled aptamer (aptamer-FAM) was designed as the signal probe, ZEN-capture probe and negative charge reactor. In the absence of ZEN, the negatively charged aptamer-FAM combined with the positively charged MSNs-NH 2 in an electrostatic manner. In the presence of ZEN, the fluorescence intensity in the supernatant increased significantly because the aptamer-FAM could bind to ZEN instead of MSNs-NH 2 . Under the optimal experimental conditions, this assay exhibited excellent specificity, repeatability and a wide linearity range of 0.005–150 ng/mL, with a detection limit of 0.012 ng/mL. Additionally, it showed high recovery (83.3–101.5%) for the spiked samples. There was no statistically significant difference in the ZEN concentrations detected by the proposed assay and HPLC in naturally contaminated samples. Overall, this design provides a new strategy for the rapid, inexpensive and sensitive detection of ZEN, and it could be applied to develop fluorometric assays for different targets by the selection of appropriate aptamers.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2020
·Vol. 31
·期 15
·12765-12774
·
IF 2.22
Liu Yue,Cai Hongkun,Chu Yinhuan,Su Jian,Ye Xiaofang,Ni Jian,Li Juan,Zhang Jianjun
Poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) is widely used as a hole transport materials in perovskite solar cells, due to a variety of suitable properties. However, the existence of the insulated PSS chain leads to the low electrical conductivity, which should be improved for the further development of inverted perovskite solar cells. In this study, three alcohol solvents were proposed for post-treatment of PEDOT:PSS thin films, which utilized the electrostatic shielding effect of alcohol molecules to promote phase separation of the PEDOT chain and the PSS chain. At the same time, The insulated PSS chain was removed by dissolving in highly hydrophilic alcohols. In the end, we successfully improved the conductivity of PEDOT:PSS thin films. By ethanol solvent treatment, the short-circuit current density of the perovskite solar cell based on glass/ITO/PEDOT:PSS was increased from 15.64 to 17.45 mA/cm 2 , the power conversion efficiency (PCE) reached 11.36%, which was 25% higher than the traditional structure. For flexible devices, a preliminary PCE of 9.20% was achieved.
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