ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Zhu Ruitao,Wang Zhiqiang,Liang Aihui,Wen Guiqing,Jiang Zhiliang
A novel gold nanocluster surface molecularly imprinted polyisopropylacrylamide probe (AuNC@MIP) was synthesized for the specific detection of NO 2 − , using N-isopropylacrylamide (NIPAM) as the functional monomer, gold nanoclusters (AuNC) as the substrate, ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent, and nitrosophenol (NPN) produced from sodium nitrite (NaNO 2 ) and phenol (PN) as the template molecule, N,N-dimethylformamide (DMF) as the solvent, and azobisisobutyronitrile (AIBN) as the initiator. The nanoprobe was characterized using molecular spectroscopy, XPS, TEM, TGA, and zeta potential analysis. The probes revealed a prominent resonance Rayleigh scattering (RRS) peak at 370 nm. Upon addition of NO 2 − , the RRS intensity at 370 nm decreased due to the RRS energy transfer enhancing. The linear determination range is 0.125–1.50 nmol/L NO 2 − , with a limit of detection (LOD) of 0.085 nmol/L. The new RRS method was applied to determine NO 2 − in meat, dairy products, and water samples, with recovery of 96–107% also relative standard deviations (RSDs) of 1.1–8.0%.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 28
·6621-6638
·
IF 3.8
Chen Chang,Zheng Ting,Chen Yang,Li Zegeng,Wu Huan
The preparation of cellular metabolomics samples and how to achieve comprehensive coverage of different polar metabolites in cell samples in the analysis pose a challenge for cellular metabolomics. In this study, we optimized a metabolomics protocol based on ultra-high-performance liquid chromatography high-resolution mass spectrometry (UPLC/HRMS) for the extraction and detection of metabolites in A549 cells and exploration of the intervention effect of Qi-Yu-San-Long decoction (QYSLD) on A549 cells. The results indicate that the lowest level of ATP leakage was observed when A549 cells were quenched under liquid nitrogen. MeOH/chloroform/H 2 O (1:2:1) extraction yielded more chromatographic peaks and excellent reproducibility, and the relative extraction efficiency of most target metabolites was also high. And we optimized the chromatographic separation conditions in both HILIC and RPLC modes, enabling comprehensive detection and analysis of metabolites with varying polarities. Then, we applied the optimized method to UPLC-Q-TOF/MS-based metabolomics of A549 cells to study the mechanism of QYSLD intervention in non-small cell lung cancer (NSCLC). The CCK-8, EdU staining, and cell cycle assay showed that QYSLD inhibited the proliferation of A549 cells by interfering with the cell cycle and blocking them in the G1 phase. A total of 36 differential metabolites associated with the antitumor effects of QYSLD on NSCLC were identified, mainly involving nicotinate and nicotinamide metabolism, sphingolipid metabolism, and glycerophospholipid metabolism. And western blotting confirmed that the change in 1-methylnicotinamide levels after QYSLD intervention was associated with the inhibition of nicotinamide N-methyltransferase expression in A549 cells. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-23
·
IF 12.6
Xia Guoyu,Fan Zhongxiong,Wang Qingluo,Li Jianmin,Zhang Yuxiang,Aipire Adila,Su Qiurong,Li Ying,Hou Zhenqing,Li Jinyao
Photodynamic-induced immunotherapy (PDI) is often hampered by low reactive oxygen species (ROS) yield, intra-tumor hypoxia, high glutathione (GSH) concentration, and immunosuppressive microenvironment. In view of this, a ruthenium (Ru)-based nanobattery (termed as IRD) with cascade-charged oxygen (O 2 ), ROS, and photodynamic-induced immunotherapy by coordination-driven self-assembly of transition-metal Ru, photosensitizer indocyanine green (ICG), and organic ligand dithiobispropionic acid (DTPA). Then, IRD is camouflaged with macrophage membranes to obtain a nanobattery (termed as IRD@M) with targeting and immune evasion capabilities. Upon intravenous administration, IRD@M with a core-shell structure, nano diameter, and good stability can specifically hoard in tumor location and internalize into tumor cells. Upon disassembly triggered by GSH, the released Ru³⁺ not only catalyzes the conversion of endogenous hydrogen peroxide (H₂O₂) into O₂ to alleviate tumor hypoxia and reduce the expression of hypoxia-inducible factor-1 α (HIF-1 α ), but also generates hydroxyl radicals (·OH) to elevate intracellular ROS levels. This process significantly enhances the photodynamic therapy (PDT) efficacy of the released ICG. Meanwhile, the released DTPA can significantly downregulate overexpressed GSH to reduce the elimination of ROS deriving from PDT by the exchange reaction of thiol-disulfide bond. It is also found that alleviating the hypoxic tumor microenvironment synergistically enhances the PDT efficacy, which in turn cascades to recharge the subsequent immune response, significantly improving the immunosuppressive tumor microenvironment and activating systemic tumor-specific immunity. Notably, in vitro and in vivo experimental results jointly confirm that such cascade-recharged macrophage-biomimetic Ru-based nanobattery IRD@M can achieve an obvious tumor elimination while results in a minimized side effect. Taken together, this work highlights a promising strategy for simple, flexible, and effective Ru-based immunogenic cell death (ICD) agents within PDI. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-13
·
IF 3.8
Zhou Liuyi,Chen Lulu,Li Ting,Wang Lu,Lin Shiqi,Zhao Ye,Wu Sufan,Jin Tingting
Rosacea is a chronic inflammatory skin disease that typically affects the central facial area. Its main clinical symptoms include paroxysmal flushing, telangiectasia, and non-temporary erythema. Cell-free adipose tissue extracts (ATEs) are liquid components extracted from human adipose tissue that contain large amounts of growth factors. Despite the scar-reducing, anti-aging, and wound-healing effects of ATEs, the efficacy of ATEs in rosacea remains unknown. Therefore, the anti-rosacea effects of ATEs were investigated in human cathelicidin peptide (LL-37) induced rosacea mice and capsaicin (CAP)-stimulated HaCaT keratinocytes. In vitro, ATEs significantly reduced TRPV1 expression, intracellular calcium ions influx and the release of inflammatory factors (such as KLK5, IL-6, IL-8 and TNF-α) after intervening in CAP-stimulated cells. The in vivo results revealed that ATEs alleviated rosacea symptoms, such as erythema score, erythema area, transepidermal water loss, abnormal epidermal thickness, mast cell infiltration and telangiectasia upon downregulating TRPV1 and CD31 expression. Moreover, the up-regulated TRPV1 protein expression was also recovered by ATEs administration in vivo and in vitro. Meanwhile, ATEs demonstrated good biocompatibility. In summary, ATEs could be a potential therapeutic agent for rosacea by regulating inflammation and alleviating telangiectasia.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2025
·
IF 4.8
Bai Xiaoyu,Zhou Yuan,Li Jiangtao,Xia Zhaoxia,Wang Zhaojiang,Zhang Qiang,Jiang Yuanzhang,Wang Guixin,Tan Lin,Zhang Yong
In this paper, cellulose acetate (CA) and cellulose nanocrystal (CNC) modified polyvinylidene fluoride (PVDF) membranes were prepared by solution blending and non-solvent induced phase separation methods to improve the hydrophilicity and diafiltration capacity of the membranes. The changes in membrane performance before and after modification were systematically studied through simulated dialysis experiments, antifouling performance tests and biocompatibility analysis. The results show that CA blending and CNC doping will change the morphology and structure of the PVDF membrane, improve its hydrophilicity and permeability, and make it show better filtration capacity and antifouling performance. The modified PVDF membrane has higher clearance and diafiltration efficiency for small molecular solutes urea and creatinine, especially for the middle molecular solute lysozyme, while retaining most of the protein molecules, with a flux recovery rate of more than 95%. The modified PVDF membrane also has good biocompatibility. Bio-based CA and CNCs play an important role in improving the antifouling and diafiltration capacity of PVDF membranes.
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 3
·1-18
·
IF 2.4
Kong Lingnan,Abulizi Abudula,Cong Jingjing,Meng Weihan,Chang Yaqing,Sun Zhihui
The cytochrome P4503A ( CYP3A ) subfamily has been well characterized in vertebrates, where it plays a pivotal role in xenobiotic detoxification and the regulation of steroid hormone levels. However, their molecular features and functions in invertebrates remain poorly understood. The sea cucumber ( Apostichopus japonicus ) is not only a commercially significant mariculture species but also an excellent model for studying organ development and evolutionary biology. In this study, we identified seven CYP3A genes in A. japonicus using gonadal transcriptomic data. These genes exhibited ubiquitous expression across adult tissues, with significantly higher levels in ovarian tissues compared to testes tissues. Protein sequence analysis revealed that the catalytic residues Ala 306 -Thr 310 -Cys 437 , which are critical for aromatase activity, are highly conserved. This suggests that CYP3A proteins may mediate aromatization-like reactions similar to CYP19A in vertebrates. Additionally, dietary administration of the aromatase inhibitor letrozole led to histological changes in the testes and a reduction in the diameter of large oocytes in the ovaries. These findings provide valuable insights into the molecular mechanisms underlying gonadal development in sea cucumbers and lay the groundwork for future research on the artificial induction of sex reversal in echinoderms.
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL GENETICS
·
2025
·
IF 1.6
Li Qiaoxia,Zhang Yongyan,Shen Haoyu,Wang Ziqian,Huang Jiezhuang,Tang Shuli,Chen Peiyue,Zhi Zhifu
Intrauterine adhesions (IUA) is one of the most prevalent gynecological conditions affecting women of childbearing age. The active ingredient curcumin (CUR), derived from turmeric, is a promising candidate for the treatment of IUA. Nevertheless, the mechanism of action remains undetermined. This study investigates the role and mechanism of CUR in the treatment of IUA through network pharmacology, molecular docking, and molecular biology experiments. IUA-related targets were sourced from the GeneCards database. CUR-related targets were obtained from Herb and SwissTarget Prediction. Cytoscape version 3.10.2 was employed to construct PPI networks and to identify core targets. GO and KEGG enrichment analyses were conducted using the DAVID database. Additionally, molecular docking was employed to evaluate the interaction between CUR and core targets. Finally, the mechanism and targets of CUR in IUA were validated through animal experiments. A total of 122 common target points for CUR and IUA were identified. Topological analysis and KEGG analysis identified 20 core target points, encompassing multiple pathways, including inflammation and the PI3K/AKT signaling pathway. Molecular docking results demonstrated that CUR exhibits a strong binding affinity for the core target points. In vivo experiments indicate that CUR significantly alleviates the fibrosis and epithelial-mesenchymal transition (EMT) processes of the endometrium in IUA rats while inhibiting the overexpression of TGF-β1 in the uterine tissue of IUA rats and the activation of the PI3K/AKT and TLR4/NLRP3 signaling pathways. CUR can inhibit fibrosis and the EMT process in the endometrium of IUA rats, and its mechanism may be associated with the inhibition of the PI3K/AKT and TLR4/NLRP3 signaling pathways.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science China-Chemistry
·
2025
·
IF 9.7
Zhang Wen,Li Jiaxin,Wang Nan,Li Muzi,Peng Chen,Zhang Xinyue,Ouyang Guanghui,Li Yan
Lipid nanoparticles (LNPs) are non-viral nucleic acid delivery systems that show great potential in vaccine development and disease treatment. Although LNPs are particularly advantageous for in vivo delivery, the wide application of LNPs is impeded as their systemic delivery of nucleic acid drugs to extrahepatic tissues remains highly challenging. To address this issue, we developed lung-targeted polyzwitterionic LNPs with zwitterionic polymer poly(2-methyacryloyloxyethyl phosphorylcholine) (PMPC) modified 1,2-dimyristoyl-sn-glycerol lipid for the delivery of small interfering RNA (siRNA). Three libraries with 90 PMPC-LNPs@siRNA were established. The polyzwitterionic PMPC-LNPs had high siRNA encapsulation efficiency of about 90%. The findings revealed that polyzwitterionic PMPC-LNPs@siRNA absorbed protein corona with the main component of Vitronectin, mediating lung-targeted delivery of siRNA. With good cellular uptake and endo/lysosomal escape ability, in vitro and in vivo studies demonstrated that polyzwitterionic PMPC-LNPs with siRNA against tumor necrosis factor-α (TNF-α) could significantly down-regulate the TNF-α in mRNA and protein levels, and improved the pathological features of lung inflammation. Polyzwitterionic PMPC-LNPs@siRNA achieved safe and efficient treatment of lung inflammation. Therefore, this work offered a promising siRNA therapeutic approach for lung diseases.
✓ 已验证
产品关联
DOI
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2025
·Vol. 41
·期 4
·1-15
·
IF 4.2
Zhao Jing-yi,Gao Fan,Wu Mengru,Li Yang,Chen Yong,Xiao Zijun
Enantiopure ( S )-2-methylbutanoic acid [( S )-2-MBA] is a high-value chiral compound with applications in fragrances, pharmaceuticals, and agrochemicals. However, conventional chemical synthesis lacks stereoselectivity, while existing biosynthetic methods suffer from low yield and purity. Here, we report a novel microbial process using Bacillus spizizenii ATCC 6633 for efficient ( S )-2-MBA production via L-isoleucine catabolism. Through targeted screening of rhizospheric soil isolates and Bacillaceae strains, ATCC 6633 demonstrated superior performance, producing 3.67 g/L ( S )-2-MBA with 99.32% enantiomeric excess ( ee ) under optimized conditions (45 °C, 8% inoculation, 5 g/L glucose, and 8 g/L L-isoleucine). A 58.92% conversion efficiency was achieved, and a simplified purification process recovered 63.90% product with 97.32% purity. Mechanistic studies suggested glucose depletion triggered ( S )-2-MBA accumulation, aligning with starvation-induced secondary metabolism. This cost-effective, eco-friendly approach eliminates racemic separation steps and harsh reagents, positioning ATCC 6633 as a promising biocatalyst for sustainable ( S )-2-MBA production.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microsystems & Nanoengineering
·
2024
·Vol. 10
·期 1
·1-10
·
IF 7.3
Yang Ziwei,Wang Jingxiao,Wan Xiao,Xu Hongcheng,Zhang Chuanyu,Lu Xiaoke,Jing Weixuan,Guo Chuanfei,Wei Xueyong
High-sensitivity flexible pressure sensors have obtained extensive attention because of their expanding applications in e-skins and wearable medical devices for various disease diagnoses. As the representative candidate for these sensors, the iontronic microstructure has been widely proven to enhance sensation behaviors such as the sensitivity and limits of detection. However, the fast and tunable fabrication of ionic-porous sensing elastomers remains challenging because of the current template-dissolved or 3D printing methods. Here, we report a microbubble-based fabrication process that enables microporous and resilient-compliance ionogels for high-sensitivity pressure sensors. Periodic motion sliding results in a relative velocity between the imported airflow and the fluid solution, converts the airflow to microbubbles in the high-viscosity ionic fluid and promptly solidifies the fluid into a porous ionogel under ultraviolet exposure. The ultrahigh porosity of up to 95% endows the porous ionogel with superelasticity and a Young’s modulus near 7 kPa. Due to the superelastic compliance and iontronic electrical double-layer effect, the porous ionogel packaged into two electrodes endows the pressure sensor with high sensitivity (684.4 kPa −1 ) over an ultrabroad range (~1 MPa) and a high-pressure resolution of 0.46%. Furthermore, the pressure sensor successfully captures high-yield broad-range signals from the fingertip low-pressure pulses (<1 kPa) to foot high-pressure activities (>500 kPa), even the grasping force of soft machine hands via an array-scanning circuit during object recognition. This microbubble-based fabrication process for porous ionogels paves the way for designing wearable sensors or permeable electronics to monitor and diagnose various diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Death & Disease
·
2024
·Vol. 15
·期 10
·1-16
·
IF 8.1
Wan Xinyi,Zeng Weiwu,Fan Haonan,Wang Chenliang,Han Shixun,Sun Zhongxing,Tang Mei,Shao Juejia,Liu Yu,Fang Yuan,Jia Junqi,Tang Yin,Zhang Yanjun,Zhao Bin,Fang Dong
MAT2B works together with MAT2A to synthesize S-Adenosyl methionine (SAM) as the primary methyl donor. MAT2B, despite lacking catalytic activity, exerts regulatory control over the enzymatic activity of MAT2A. In addition to the enzymatic activity regulation, we find that, in an NADP + -dependent manner, MAT2B binds and stabilizes MAT2A. Disruption of the cellular NADP + remodels the protein level of MAT2A. The pentose phosphatase pathway regulates the level of MAT2A protein through the interaction of NADP + with MAT2B. Additionally, MAT2B-MAT2A interaction regulates the mRNA m6A modification and stability. In liver tumors, the Mat2a mRNA level is elevated but the protein level is decreased by the restricted NADP + . Blocking the interaction between MAT2B and MAT2A by the keto diet can suppress liver tumor growth. These findings reveal that MAT2B is essential for regulating the protein levels of MAT2A and connecting SAM synthesis to mRNA m6A.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Reaction Kinetics Mechanisms and Catalysis
·
2024
·
IF 1.7
Yan Jiaqi,Fu Weiwen,You Kuiyi,Zhao Fangfang,Luo He’an
A solvent-free, acid-free, and efficient strategy was developed for the selective preparation of 4-nitro-o-xylene (4-NOX) from the catalytic nitration of o-xylene with NO 2 mediated O 2 over BiCl 3 immobilized silicic acid catalyst (BiCl 3 -SA). The results indicated that the Lewis acid BiCl 3 -SA conjoined NO 2 –O 2 as a composite system synergistically promotes o-xylene conversion and 4-NOX selectivity. Under optimal conditions, 52.4% of o-xylene conversion with 68.4% 4-NOX selectivity was obtained at 35 °C. The characterization demonstrated that the highly dispersed metal Bi species form stable chemical bonds with the SA surface, which can effectively inhibit the loss of metal species and generate abundant acid sites. The developed catalyst is not only inexpensive but also has excellent stability and catalytic performance. Furthermore, a plausible mechanism for the catalytic nitration of o-xylene using NO 2 as a nitration agent over BiCl 3 -SA was proposed. This work provides an eco-friendly and practical protocol for improving desirable 4-NOX selectivity and reducing the discharge of acidic wastewater, with potential application prospects. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）