JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 55
·期 3
·1117-1125
·
IF 3.442
Han Jishu,Liu Ying,Wang Ying,Zhao Ruiyang,Wang Lei
The development of novel photocatalyst is an important issue for improving the photocatalytic hydrogen production efficiency. Herein, novel aqueous ternary Cd x Mo 1− x Se quantum dots (QDs) are prepared with five ratios ( x = 0, 0.5, 0.67, 0.75, 1) by modulating the metal content. Experimental results show that the doping of Mo promotes the photoinduced electrons transfer and decreases the photoinduced electron–hole pairs recombination. Compared with the CdSe QDs, Cd 0.75 Mo 0.25 Se QDs, Cd 0.5 Mo 0.5 Se QDs and MoSe 2 QDs, Cd 0.67 Mo 0.33 Se QDs exhibit significantly enhanced photocatalytic activity, and the amount of H 2 production comes up to 911.1 μmol in 7 h. Meanwhile, the Cd 0.67 Mo 0.33 Se QDs display substantially high stability in recycling test, which facilitates the utilization of ternary QDs in the photocatalytic hydrogen production application.
✓ 已验证
产品关联
DOI
高影响因子
Nature Chemical Biology
·
2019
·Vol. 15
·期 11
·1110-1119
·
IF 12.154
Wang Pengchong,Tang Wei,Li Zeyao,Zou Zhongyu,Zhou Ying,Li Ran,Xiong Tianyu,Wang Jianbin,Zou Peng
RNA molecules are highly compartmentalized in eukaryotic cells, with their localizations intimately linked to their functions. Despite the importance of RNA targeting, our current knowledge of the spatial organization of the transcriptome has been limited by a lack of analytical tools. In this study, we develop a chemical biology approach to label RNAs in live cells with high spatial specificity. Our method, called CAP-seq, capitalizes on light-activated, proximity-dependent photo-oxidation of RNA nucleobases, which could be subsequently enriched via affinity purification and identified by high-throughput sequencing. Using this technique, we investigate the local transcriptomes that are proximal to various subcellular compartments, including the endoplasmic reticulum and mitochondria. We discover that messenger RNAs encoding for ribosomal proteins and oxidative phosphorylation pathway proteins are highly enriched at the outer mitochondrial membrane. Due to its specificity and ease of use, CAP-seq is a generally applicable technique to investigate the spatial transcriptome in many biological systems.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 6
·1343-1351
·
IF 3.637
Zhang Lei,Wang Junping,Deng Jiankang,Wang Shuo
In this study, a novel fluorescent “turn-on” aptasensor was developed for sensitive and rapid detection of tetracycline (TC) in animal-derived food. It is based on aptamer-functionalized nitrogen-doped graphene quantum dots (N-GQDs-aptamer) coupled with cobalt oxyhydroxide (CoOOH) nanoflakes. The CoOOH nanoflakes are efficient fluorescence quenchers in homogeneous solutions, and this is due to their advantages of excellent optical properties, superior flexibility, and water dispersibility. The proposed method’s mechanism is driven by quenching based on the fluorescence resonance energy transfer (FRET) between the donor (N-GQDs) and the acceptor (CoOOH nanoflakes). On the other hand, fluorescence recovery is caused by the structure switching behavior of the aptamer. Compared with previous methods, our developed method exhibits better behavior in terms of being easy to fabricate and being simple in detection procedure and maintains the detection limit low enough in TC determination: a linear range from 1 to 100 ng mL −1 and a detection limit of 0.95 ng mL −1 (S/N = 3). Furthermore, the proposed method was applied to five animal-derived food samples (milk, honey, fish, eggs, and chicken muscle) and demonstrated practical applicability. As well, the method has the advantages of simplicity in pre-treatment and convenience in instruments, saves times, and is cost-effective. Finally, the proposed method demonstrates significant potential for sensitive and rapid detection of specific components in real samples.
Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-8
·
IF 5.479
Yi Jiecan,Wu Pian,Li Guiyin,Xiao Wen,Li Lei,He Yayuan,He Yafei,Ding Ping,Chen Cuimei
An aptamer-based assay is described for the determination of Salmonella typhimurium ( S. typh ). Carboxymethyl chitosan was loaded with amino-modified aptamer against S. typh , and then adsorbed on gold nanoparticles by electrostatic interaction to form a composite that acts as the molecular recognition element. In the presence of S. typh , it will be bound by the aptamer, and this changes the structure of the recognition element. On addition of salt solution, the gold nanoparticles agglomerate so that the color of the solution changes from red to blue. S. typh can be detected via measurement of the absorbance at 550 nm. Absorbance increases linearly with the logarithm of the S. typh concentration in the range from 100 to 10 9 cfu·mL −1 . The limit of detection is 16 cfu·mL −1 . The specificity and practicability of the assay were evaluated. The recoveries of S. typh from spiked milk samples are between 92.4 and 97.2%. The analytical results are basically consistent with those of a plate counting method.
Schematic representation of the colorimetric assay for Salmonella typhimuium ( S . typh ) using carboxymethyl chitosan (CMCS)-aptamer (Apt)-gold nanoparticles (AuNPs) composites.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 27
·期 3
·2588-2598
·
IF 2.914
Yuan Donghai,Zhang Wenjing,Cui Jun,He Liansheng,Wang Jiazhuo,Yan Chenling,Kou Yingying,Li Junqi
Magnetic phosphorylated chitosan composite (P-MCS), an excellent adsorbent for Co(II), was synthesized in this experiment via a facile fabrication. Its removal efficiency was improved by optimizing pH, contact time, and initial concentration. The adsorption isotherms and kinetic models of Co(II) by P-MCS followed the Langmuir model and the pseudo-second-order model, respectively. However, the rate of adsorption was also affected by intragranular diffusion. The maximum adsorption capacity was 46.1 mg g-1 for Co(II). The results of spectroscopic analysis also indicated that good adsorption performance of Co(II) mainly depends on surface chelation between functional groups and metal ions. The saturation magnetic susceptibilities of P-MCS and P-MCS-Co were 22.29 emu g −1 and 18.18 emu g −1 , respectively. The excellent magnetic properties of P-MCS enabled the easy achievement of solid-liquid separation via the use of an external magnetic field. In complex aqueous solutions, K + , Na + , Ca 2+ , and Mg 2+ have less influence on P-MCS adsorption Co(II), but the adsorption capacity on Co(II) is still good. This study shows the feasibility of using P-MCS to treat wastewater containing Co(II).
✓ 已验证
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2019
·Vol. 104
·期 1
·149-155
·
IF 1.65
Xie Yan-Li,Jiang Wayne,Li Fen,Zhang Yu,Liang Xiao-Yu,Wang Meng,Zhou Xueqing,Wu Shao-Ying,Zhang Cheng-Hui
This study was intended to develop an environment-friendly controlled release system for spirotetramat in an alginate matrix. Four formulations, starch–chitosan–calcium alginate (SCCA), starch–calcium alginate (SCA), chitosan–calcium alginate (CCA), and calcium alginate (CA) complex gel beads, were prepared by the extrusion–exogenous gelation method. The properties of the formulations were studied. The results showed that the release behaviors of the formulations in water could be well described by the logistic model, and the release occurred through Fickian diffusion. Among the four formulations, SCCA showed the highest entrapment efficiency, drug loading and the slowest release rate. Degradation studies revealed that the SCCA formulation exhibited an obvious slower degradation rate of spirotetramat in soils than the commercially available formulation. The estimated half-life of the SCCA formulation was 2.31, 3.25, and 4.51 days in waterloggogenic paddy soil, purplish soil, and montmorillonite, respectively, when the soils were moistened to 60% of its dry weight. This study provided a possible approach to prolong the duration of spirotetramat and to reduce environmental contamination.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2019
·Vol. 298
·期 1
·103-111
·
IF 1.906
Lv Haitao,Tang Dongyan,Sun Zhaojie,Gao Jingru,Yang Xu,Jia Shuyue,Peng Jing
Shape memory polymers (SMP) with better biodegradability and better stability have great potential applications in biomedical fields, such as the drug carriers or the tissue engineering scaffolds. In this study, poly(ε-caprolactone)(PCL)-based polyurethane(PU) microfibers were fabricated with the containing of hydroxyapatite(HA) to enhance the biodegradability and to exhibit excellent shape memory performance. The composition, the morphology, the thermal stability, and the mechanical properties of the microfibers were characterized and detected using Fourier transform infrared spectroscopy (FTIR), 1 H nuclear magnetic resonance ( 1 HNMR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC). and dynamic mechanical analysis (DMA), etc. And dexamethasone was selected as drug model to investigate the delivery and release behaviors of the carrier of the microfibers. It was revealed that HA enhanced the degradation rate of the shape memory polyurethane (SMPU) fibers, and the fibers could guarantee a sustained long time drug release. The detection on the shape memory performance found that, with the different addition amounts of HA, the composite microfibers of (SMPU) and HA exhibited the different shape memory transition temperature ( T trans ) values. And with the addition of 3 wt% of HA, the excellent shape recovery ratios of R r (> 97%) and the shortest recovery time of ~ 6 s could be obtained. With further increase of the amounts of HA, the recovery force and the recovery time were reduced and prolonged, respectively. The obtained results proved that the biodegradable SMPU/HA composite microfibers have more valuable application prospects in biomedical fields.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 412
·期 2
·473-480
·
IF 3.286
Peng Shan,Bai Ligai,Shi Xiaoqian,Li Mingxue,Wang Laisen,Sun Fanrong,Liu Haiyan
A phenyl-based polymer monolithic column was prepared via free radical polymerization in a stainless steel column with the size of 4.6 mm i.d. × 50 mm, using ethylene glycol phenyl ether acrylate as the monomer. The resulting monolithic column shows high porosity of 73.42% and relative uniform pore structure, as characterized by mercury porosimetry and scanning electron microscopy, respectively. The optimized polymer monolith column was used for on-line solid-phase extraction prior to the reversed phase mode HPLC-UV analysis for the determination of dioscin in human plasma, using a COSMOSIL C18 column (4.6 mm × 150 mm, 4.5 μm). Water was used to wash non-retained components from the SPE sorbent, and methanol water (80:20, V/V) was used as the mobile phase for isocratic elution of dioscin. The maximum adsorbed quantity of dioscin to the SPE column is 6.79 mg/g, which is high enough for the quantitative analysis of dioscin in plasma, due to the low content of dioscin in plasma. The method was validated by assessing the linearity, lower limit of quantification, intra- and inter-day precision, accuracy, and repeatability. The developed method was applied for the analysis of dioscin in plasma from a volunteer who had orally administered an aqueous extract of dioscorea nipponica rhizome, showing the method capable of detecting dioscin in the plasma. These results show that the developed method is a rapid method for on-line solid-phase extraction and determination of dioscin from plasma, exhibiting good selectivity with hydrogen bond interaction and hydrophobic interaction, good clean-up ability, cost-saving, and time-saving.
Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 36
·期 5
·774-780
·
IF 1.071
Cui Min,Ren Jujie,Wen Xiaofang,Li Na,Xing Yifei,Zhang Cong,Han Yuanyuan,Ji Xueping
Superoxide anion, one of the most active reactive oxygen species, is associated with the development of many diseases. So monitoring superoxide anion in living cells is of great significance for the pathological research of many diseases. In this work, a new non-enzymatic sensor for the detection of superoxide anion(O •− 2
) was developed, which was fabricated by the nanocomposites composed of manganese(III) tetraphenyl porphine(MnTPP) as superoxide dismutase mimic and electrochemical reduced graphene oxide(ERGO) as electrode support material to modify the glassy carbon electrode(GCE). The electrochemical behavior of the fabricated electrode(MnTPP/ERGO/GCE) was performed by electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV), which revealed that MnTPP/ERGO/GCE possessed good catalytic ability to the electrochemical reduction of O •− 2
. The MnTPP/ERGO/GCE showed excellent electroanalysis performance towards O •− 2
using the technique of differential pulse voltammetry(DPV) with a linear relationship in the range of 0.2–110.0 µmol/L, a sensitivity of 445 µA·L·mmol −1 ·cm −2 and a detection limit of 0.039 µmol/L(S/N=3). The real-time monitoring of O •− 2
from MCF-7 breast cancer cells stimulated by zymosan was realized in this work, which indicates that the MnTPP/ERGO/GCE hold potential application for electrochemical quantification of superoxide anions in biological applications.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2019
·Vol. 125
·期 12
·1-7
·
IF 1.784
Cheng Xiao,Bao Chongzhi,Wang Xiaoming,Zhang Fan,Dong Wentao
Current soft surface electrodes have attracted more and more attention owing to their potential applications in the fields of biological signal monitoring, wearable human–machine interface and internet of things. The paper presents that soft dry electrode based on PDMS-CB conductive polymer is designed and fabricated to continuous, long-term, stable electrocardiogram (ECG) signal recordings which is conformal contact with the soft skin surface. The wearable electronic platform based on the soft dry electrode is built up to collect the ECG data. Integration of the soft dry electrodes with wearable electronic platform would improve the wearability and adaptability of the soft electrode in biological healthy monitoring which represents the future application in ubiquitous healthcare. Experiments have demonstrated that ECG data from the soft electrodes are recorded with PQRST wave which are similar to ECG data from Ag/AgCl gel electrodes. Meanwhile, robustness and stability of ECG signal are validated by the soft dry electrodes with different body actions.
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 27
·期 21
·25899-25907
·
IF 2.914
Yao Zongbao,Yang Liu,Song Na,Wang Changhui,Jiang Helong
As a recently discovered process of nitrogen cycling, anaerobic ammonium oxidation coupled to ferric iron reduction (Feammox) has attracted more attentions. This study investigated the spatial variation of Feammox in the sediment of different zones of a shallow freshwater lake and the effect of organic matter derived from algae and macrophyte on Feammox process. The potential Feammox rates showed significant differences among sediments from algae-dominated area (ADA), transitional area in the center of the lake (TDA), and macrophyte-dominated area (MDA), and in a descending order, ADA, MDA, and TDA. The potential Feammox rate ranged from 0.14 to 0.34 mg N kg −1 day −1 in the freshwater lake sediment. The potential Feammox rates of the sediment with algae or macrophyte amendment were 12.29% and 15.31% higher than the control test without algae and macrophyte amendment. The addition of algae or macrophyte to the sediment from TDA could improve the amount of HCl-extractable total Fe, Fe(III) reduction rate, and the abundance of FeRB. These results demonstrated that organic matter is one of the key regulators of Feammox process.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2019
·Vol. 9
·期 1
·1-7
·
IF 4.011
Li Zhen,Peng Ying,Xia Xingyu,Cao Zhe,Deng Yuqing,Tang Bin
A Sr-based metal-organic framework (MOF) is introduced as ketoprofen carrier to form a comprehensive system for treating osteoarthritis (OA), and the drug loading amount and release rate is investigated. Structural characterization of the samples showed that Sr/PTA-MOF had good crystal morphology and structure, and chemical and thermal stability. Ketoprofen was successfully loaded on the MOF carrier, which had been identified by high performance liquid chromatography (HPLC) and thermogravimetric analysis (TGA). The release experiment manifested that more than 90% of ketoprofen released from Sr/PTA-MOF after 24 h, and ketoprofen delivery was mainly governed by the Higuchi model. Furthermore, cytotoxicity experiment manifested that synthesized MOF carrier had no poisonous effect on OA chondrocytes, which provided a preliminary foundation for the realization of comprehensive treating OA.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）