BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 45
·期 5
·891-900
·
IF 3.434
Qi Zhipeng,Tong Xinyi,Zhang Xiaomeng,Lin Haifeng,Bu Su,Zhao Linguo
Dihydro-β-ionone is a characteristic aroma compound of Osmanthus fragrans and is widely applied in the flavor & fragrance industry. However, the main focus is on chemical synthesis due to the metabolic pathways of dihydro-β-ionone is still unclear. Here, we explored the one-pot synthesis system for dihydro-β-ionone production using carotenoid cleavage dioxygenase (CCD) and enoate reductase. After screening the CCD enzyme, PhCCD1 from the Petunia hybrid was identified as the suitable enzyme for the first step of dihydro-β-ionone synthesis due to the high enzyme activity for carotenoid. The PhCCD1 was expressed in Escherichia coli and further characterized. The optimal activity of PhCCD1 was observed at pH 6.8 and 45 °C. The enzyme was stable over the pH range of 6.0–8.0 and had good thermal stability below 40 °C. Then, we optimized the coupled reaction conditions for dihydro-β-ionone production by PhCCD1 and enoate reductase AaDBR1 from Artemisia annua . Furthermore, we introduced the NADPH regeneration system with a 1.5-fold enhancement for dihydro-β-ionone production. Collectively, approximately 13.34 mg/L dihydro-β-ionone was obtained by the one-pot biosystem with a corresponding molar conversion of 85.8%. For the first time, we successfully designed and constructed a new synthesis pathway for dihydro-β-ionone production in vitro. The coupled catalysis reported herein illustrates the feasibility of producing dihydro-β-ionone from carotenoids and guides further engineering in the food industry.
研究领域:
生物化学
·
合成生物学
·
芳香化合物生产
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DOI
PubMed
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2021
·
IF 4.354
Yuxiu Li,Xiangqian Li,Xiaowei Li,Dayong Shi
The straightforward synthesis of α-fluoro-β-arylalkenyl sulfones under transition-metal- and base-free conditions has been described, which displays broad functional group compatibility and high stereoselectivity. In particular, the strategy is also applied to the late-stage modification of complex natural products and drugs.
研究领域:
有机化学
·
药物化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Jiahao Shi,Yuhe Zhang,Bin Ma,Haiyang Yong,Delu Che,Chaolan Pan,Wei He,Dezhong Zhou,Ming Li
Poly(β-amino ester)s (PAEs) have been widely developed for gene delivery, and hydrophobic modification can further enhance their gene transfection efficiency. However, systematic manipulation of amphiphilicity of PAEs through copolymerization with hydrophobic monomers is time-consuming and, to some extent, uncontrollable. Here, a modular strategy is developed to manipulate the amphiphilicity of the PAE/DNA polyplexes. A hydrophobic polymer (DD-C12–122) and a hydrophilic polymer (DD-90–122) are synthesized separately and used as a hydrophobic module and a hydrophilic module, respectively. The amphiphilicity of polyplexes could be manipulated by changing the ratio of the hydrophobic module and hydrophilic module. Using the modular strategy, the PAE/DNA polyplexes with the highest gene transfection efficiency and safety profile as well as possible mechanisms are identified. The modular strategy provides a novel way to engineer the hydrophobicity of PAEs to improve their gene transfection and can be easily generalized and potentially extended to other polymeric gene delivery systems.
研究领域:
材料科学
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生物技术
·
药学
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DOI
PubMed
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2022
·
IF 6.76
Sisi Ma,Ligang Lin,Xinyang Li,Wenying Shi,Xiaofei Zhai,Jing Yang
Developing multifunctional, efficient and durable membrane for treating complex oily wastewater is highly desirable but still a challenge due to the severe membrane fouling . Herein, nanosponge membrane with 3D-macrocycle β-cyclodextrin (β-CDs) as molecular cage was manufactured by azide-alkyne click reaction for oil/water treatment and antifouling properties simultaneously. The macrocyclic ‘molecular cage’ geometry of β-CDs can induce various guest molecules into their cavities. When clickable β-CD-N 3 was fixed onto a clickable EVAL-≡ membrane surface, the hydrophilicity of the membrane was greatly improved. Furthermore, the molecular cage–grafted membrane (EVAL-g-CD) showed better antifouling performance than a pure EVAL membrane, with lower water flux decline (15%) and higher water flux recovery (91%). The flux and separation efficiency values of the EVAL-g-CD membrane were higher than 120 L·m −2 ·h −1 and 99%, respectively. The EVAL-g-CD membrane also exhibited good adsorption performance for organic pollutants owing to its cavity structure. Furthermore, the membrane showed desirable stability and its rejection remained at 99% after filtration. This proposed 3D membrane strategy based on molecular cages sheds light on the formation of hydrophilic membrane surfaces and shows great promise for potential applications such as the separation of oil-in-water emulsions.
研究领域:
材料科学
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环境工程
·
化学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yonggan Sun,Shanshan Zhang,Huijun He,Haihong Chen,Qixing Nie,Song Li,Jiaobo Cheng,Baojie Zhang,Zhitian Zheng,Shijie Pan,Ping Huang,Lu Lian,Jielun Hu,Shaoping Nie
Dietary fiber is crucial for human health mainly due to its impact on gut microbiota structure and metabolites. This study aimed to investigate the impact of Dendrobium officinale polysaccharides (DOP) and two common fibers (β-glucan and inulin) on the gut microbiome structure and metabolic profile in vitro . Fecal samples were obtained from 30 healthy volunteers, which were then individually subjected to fermentation with each type of fiber. The results revealed that all fibers were efficiently degraded by gut microbiota, with DOP exhibiting a slower fermentation rate compared to β-glucan and inulin . The fermentation of all fibers led to a significant increase in the production of short-chain fatty acids (SCFAs) and a reduction in branched-chain fatty acids (BCFAs), sulfides, phenols, and indole. Moreover, the abundance of unclassified Enterobacteriaceae , which was positively correlated with sulfide, phenols, and indole levels, was significantly reduced by all fibers. Additionally, DOP specifically promoted the growth of Parabacteroides , while β-glucan and inulin promoted the growth of Bifidobacterium and Faecalibacterium . Taken together, these findings enhance our understanding of the role of DOP, β-glucan, and inulin in modulating gut microbiota and metabolites, where the fermentation with fecal bacteria from different volunteers could provide valuable insights for personalized therapeutic approaches.
研究领域:
食品科学
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微生物学
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营养学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6
Haolan You,Yihe Song,Yi Yang,Xicheng Wang,Shiqi Pan,Junyang Huang,Qiqi Shao,Donglei Shi,Baoli Li,Jian Li,Xiaokang Li
Beta-amyloid (Aβ), the most pivotal pathological hallmark for Alzheimer's disease (AD) diagnosis and drug evaluation, was recognized by TZ095 , a high-affinity fluorescent probe developed by rational molecular design. With a TICT mechanism, TZ095 exhibited remarkable affinity with Aβ aggregates (K d = 81.54 nM for oligomers; K d = 66.70 nM for fibril) and substantial fluorescence enhancement (F/F 0 = 44), enabling real-time monitoring of Aβ in live cells and nematodes. Significantly, this work used TZ095 to construct a new protocol that can quickly and conveniently monitor Aβ changes at the cellular and nematode levels to evaluate the anti-AD efficacy of candidate compounds, and four reported Aβ-lowering drug candidates were administrated for validation. Imaging data demonstrated that TZ095 can visually and quantitatively track the effect of Aβ elimination after drug treatment. Furthermore, TZ095 excelled in ex vivo histological staining of 12-month-old APP/PS1 mouse brains, accurately visualizing Aβ plaques. Integrating CUBIC technology, TZ095 facilitated whole-brain, 3D imaging of Aβ distribution in APP/PS1 mice, enabling high-resolution in situ analysis of Aβ plaques. Collectively, these innovative applications of TZ095 offer a promising strategy for rapid, convenient, and real-time monitoring of Aβ levels in preclinical therapeutic assessments.
研究领域:
神经科学
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药物化学
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分子生物学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2014
·Vol. 21
·期 14
·8620-8630
·
IF 2.757
Gao Huipeng,Ma Jing,Xu Li,Jia Lingyun
Organic matter (OM) plays a vital role in controlling polycyclic aromatic hydrocarbon (PAH) bioavailability in soils and sediments. In this study, both a hydroxypropyl-β-cyclodextrin (HPCD) extraction test and a biodegradation test were performed to evaluate the bioavailability of phenanthrene in seven different bulk soil/sediment samples and two OM components (humin fractions and humic acid (HA) fractions) separated from these soils/sediments. Results showed that both the extent of HPCD-extractable phenanthrene and the extent of biodegradable phenanthrene in humin fraction were lower than those in the respective HA fraction and source soil/sediment, demonstrating the limited bioavailability of phenanthrene in the humin fraction. For the source soils/sediments and the humin fractions, significant inverse relationships were observed between the sorption capacities for phenanthrene and the amounts of HPCD-extractable or biodegradable phenanthrene ( p < 0.05), suggesting the importance of the sorption capacity in affecting desorption and biodegradation of phenanthrene. Strong linear relationships were observed between the amount of HPCD-extractable phenanthrene and the amount degraded in both the bulk soils/sediments and the humin fractions, with both slopes close to 1. On the other hand, in the case of phenanthrene contained in HA, a poor relationship was observed between the amount of phenanthrene extracted by HPCD and the amount degraded, with the former being much less than the latter. The results revealed the importance of humin fraction in affecting the bioavailability of phenanthrene in the bulk soils/sediments, which would deepen our understanding of the organic matter fractions in affecting desorption and biodegradation of organic pollutants and provide theoretical support for remediation and risk assessment of contaminated soils and sediments.
研究领域:
环境科学
·
污染生态学
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DOI
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2024
·
IF 2.3
Bing Shao,Yang Fu,Bo Li,Siming Huo,Jiayu Du,Xuliang Zhang,Xin Yin,Yanfei Li,Zheng Cao,Miao Song
Faced with infectious bone defects, the development of a thermosensitive hydrogel containing icariin (ICA) represents a promising therapeutic strategy targeting infection control and bone regeneration. In this study, we prepared and evaluated the physicochemical properties, in vitro and in vivo drug release, antimicrobial activity, anti-inflammatory properties, and bone repair effects of ICA/Chitosan/β-Glycerophosphate (ICA/CTS/β-GP) thermosensitive hydrogel. Our findings demonstrate that the ICA/CTS/β-GP thermosensitive hydrogel undergoes a liquid-to-gel transition at body temperature, which is crucial for maintaining local drug release at the defect site. Additionally, the hydrogel exhibited sustained release of ICA over 28 days, showing high antimicrobial activity against Staphylococcus aureus and good biocompatibility in blood compatibility tests. In a canine model of infectious bone defects, the ICA/CTS/β-GP thermosensitive hydrogel showed effective infection control and modulated inflammation, vascular formation, and bone factor expression, while also activating the Wnt/β-catenin signaling pathway. In conclusion, the ICA/CTS/β-GP thermosensitive hydrogel could control infection and repair bone tissue. Its antimicrobial and osteogenic properties provide hope for its clinical application.
研究领域:
生物医学工程
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药剂学
·
骨科
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 18
·9611-9617
·
IF 2.936
Ji-Wei Shen,Zhiqing Wang,Xiaoxuan Wei,Jiawei Liu,Yinmao Wei
Herein, user-friendly control of the synthesis of sub-10 nm hexagonal (β-) NaYbF4:Er nanocrystals (NCs) with extremely low-level Gd3+ doping (0%, 10 mol%) was achieved. We reveal for the first time that the effective sodium/fluoride levels during the formation of cubic (α-) nuclei are not only controlled by the sodium/fluoride to rare-earth precursor ratios used, but also sensitively restricted by the in situ NaF generation reaction in a sodium oleate-based solvothermal system. Excessive in situ NaF generation will lead to a respective sodium- and fluoride-deficient environment, delayed α-to-β transition and larger β-NCs. Based on these effects, sub-10 nm monodisperse low-level Gd3+-doped β-NaYbF4:Er was obtained with a user-friendly low fluoride dosage by finely balancing this NaF generation reaction and achieving an intrinsic optimized sodium-fluoride level for NC nucleation. Notably, our work represents the first example where the focus is on the competing in situ NaF generation reaction and its use for nucleation regulation, as well as for the user-friendly control of the solvothermal synthesis of sub-10 nm β-NaYbF4:Er.
研究领域:
材料科学
·
纳米技术
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DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Shu-shu Zhong,Jun Zhang,Ze-hua Liu,Zhi Dang,Yu Liu
Arylsulfatase and β-glucuronidase are two important enzymes in humans, which play an important role in the dynamic equilibrium of steroidal estrogens. This work probably for the first time reported that hydrogen peroxide (H2O2), hypochlorite, and peracetic acid (PAA) could effectively inhibit the activities of arylsulfatase and/or β-glucuronidase. The 50% of inhibitions (IC50) of H2O2, hypochlorite, and PAA on arylsulfatase were found to be 142.90 ± 9.00, 91.83 ± 10.01, and 43.46 ± 2.92 μM, respectively. The corresponding IC50 values of hypochlorite and PAA on β-glucuronidase were 704.90 ± 41.40 and 23.26 ± 0.82 μM, whereas H2O2 showed no inhibition on β-glucuronidase. The inhibitions of arylsulfatase and/or β-glucuronidase by these three chemicals were pH-dependent. It was further revealed that the inhibitions of hypochlorite on both arylsulfatase and β-glucuronidase were irreversible. On the contrary, the inhibitions by H2O2 and PAA were reversible. In addition, the inhibition by H2O2 was competitive and that by PAA was noncompetitive. In general, H2O2 and hypochlorite can be endogenously produced in humans, which suggested that the two compounds are potential endocrine disruption compounds (EDCs) as they can cause endocrine disruption via the inhibition of arylsulfatase and β-glucuronidase. This work further indicated that any agent that can induce the production of H2O2 or hypochlorite in humans is a potential EDC, which explains why some EDCs with very weak or no estrogenic potency can cause endocrine disruption, which is confirmed in epidemiological studies.
研究领域:
生物化学
·
医学
·
环境健康科学
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DOI
PubMed
高影响因子
FASEB JOURNAL
·
2024
·Vol. 38
·期 17
·e23861
·
IF 4.4
Peimin Lin,Jie Xu,Fan Yang,Dan Li,Rong Zhang,Yongxiang Jiang,Tianyu Zheng
Recently, amyloid-β oligomers (AβOs) have been studied as the primary pathogenic substances in Alzheimer's disease (AD). Our previous study revealed that the Aβ expression level is closely related to ARC progression. Here, we demonstrated that the accumulation of AβOs in the lens epithelium of age-related cataract (ARC) patients increased during ARC progression and that this alteration was consistent with the changes in mitochondrial function, oxidative stress, and cellular apoptosis. In vitro, human lens epithelial cells (HLECs) treated with AβOs exhibited Ca 2+ dyshomeostasis, impaired mitochondrial function, elevated oxidative stress levels, and increased apoptosis. Moreover, the proapoptotic effect of AβOs was alleviated after the uptake of mitochondrial Ca 2+ was inhibited. These results establish that AβOs may promote HLEC apoptosis by inducing mitochondrial Ca 2+ overload, thus preliminarily revealing the possible association between the accumulation of AβOs and other pathological processes in ARC.
研究领域:
眼科学
·
神经退行性疾病
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细胞生物学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 8
·1951-1959
·
IF 3.8
Sun Shuo,Chen Changyu,Fu Xingyu,Zhang Yandong,Wu Xinyue,Hao Junkai,Feng Jing,Hu Lei,Yao Wenli,Yan Zhengquan
To conveniently monitor bioactive cysteine (Cys) and Fe 2+ in practice, a kind of poly-β-cyclodextrin strengthen praseodymium oxide (Pr 6 O 11 ) porous oxidase mimic ( p -β-CD@Pr 6 O 11 ) was constructed by virtue of the strong coordination between nano Pr 6 O 11 and poly-β-cyclodextrin substrate. After its microstructure and physicochemical property were characterized in detail, it was noted that porous p -β-CD@Pr 6 O 11 exhibited excellent enzyme-like catalytic activity to accelerate the oxidation of 3,3′,5,5,′-tetramethylbanzidine (TMB) and 2,2′-azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid) ammonium salt (ABTS) with significant color-enhancement effect in the air. Based on the signal amplification, trace Cys could exclusively deteriorate the UV-vis absorbance at 653 nm of p -β-CD@Pr 6 O 11 -TMB and Fe 2+ alter the one at 729 nm of p -β-CD@Pr 6 O 11 -ABTS with visual color changes. Under the optimized conditions, the proposed p -β-CD@Pr 6 O 11 -TMB and p -β-CD@Pr 6 O 11 -ABTS systems were successfully applied for dual-channel monitoring of Cys in Cys capsules and fetal bovine serum and Fe 2+ in agricultural products with quite low detection limits, i.e., 7.8×10 −9 mol·L −1 for Cys and 6.93×10 −8 mol·L −1 (S/N=3) for Fe 2+ , respectively. The synergetic-enhancement detection mechanisms to Cys and Fe 2+ were also proposed. Graphical
研究领域:
分析化学
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材料科学
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生物化学
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纳米技术
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环境监测
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生物传感器
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）