Scientific Reports
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2025
·Vol. 15
·期 1
·1-14
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IF 3.9
Zhou Lei,Zhang Mengting,Sun Huinan,Duan Liyuan,Meng Qingwei
This study establishes the first reversed-phase HPLC-UV method for simultaneous baseline separation of 12 structurally diverse water-soluble vitamins (WSVs) within 25 min. The eco-friendly approach utilizes a methanol–potassium dihydrogen phosphate (pH 4.85) gradient on a ChromCore C18 column (250 × 4.6 mm, 5 μm) with dual-wavelength detection (210/266 nm), eliminating environmentally detrimental ion-pairing reagents. Rigorous validation per ICH Q2(R1) guidelines confirmed excellent linearity (R² > 0.999), precision (RSD < 2.1%), accuracy (spike recoveries: 93.9–106.8%), and sensitivity (LODs: 0.01–0.78 µg/mL). The method resolved key analytical challenges including polarity disparities, matrix interference, and vitamin instability. Successful quantification of endogenous vitamins in DMEM medium showed ≤ 5.1% deviation from certified values for 11/12 analytes. Applied to five commercial MDCK cell culture media, the method revealed significant inter-product variability in vitamin composition – notably universal under-supplementation of riboflavin (0.576–0.741 µg/mL, 10× lower than other vitamins) and inconsistent biotin/adenine supplementation. This robust, cost-effective platform provides biomanufacturers with a powerful quality control tool for optimizing vitamin supplementation in vaccine production systems, addressing a critical gap in cell culture analytics.
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Advanced Healthcare Materials
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2025
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IF 9.6
Fengjie Liu,Fansu Meng,Xiaoshan Hong,Anil K Giri,Mulazim Hussain Asim,Zhong Chen,Yiqi Chen,Yixuan Lin,Lulu He,Qiaowen Bu,Yong Huang,Xiaoli Sun,Zhenjiang Yang,Yu Cai
The inflammatory microenvironment (IME) is closely related to the progression of triple-negative breast cancer (TNBC). As an anti-inflammatory active compound, poor solubility and targeting limit the potential of baicalin (BA) to modulate IME in TNBC. In this study, BA loaded bifunctional albumin nanoparticles (BANP) with long circulation/acid-sensitive release and active targeting functions are developed to improve the delivery efficiency of BA. BANP have a spherical core–shell structure, with appropriate particle size (79.63 ± 1.91 nm), zeta potential (−33.7 ± 0.82 mV), and encapsulation efficiency (72.71%), and are released faster in an acidic environment. BANP can enhance the uptake by TNBC cells and “M2” macrophages, prolong circulation time in the blood, and increase accumulation at tumor sites in vivo. It induces cell cycle arrest and early apoptosis in MDA-MB-231 cells and inhibits tumor growth in 4T1 tumor-bearing mice. Additionally, BANP reduce tumor immune infiltration, promote macrophage recruitment and anti-inflammatory M2 polarization, and enhance the phagocytic activity of M2 macrophages. Transcriptome sequencing and cytokine profile analysis reveal that BANP regulate TNBC IME through multitarget mechanisms related to cell cycle, inflammatory response, metabolism, etc. In summary, this study provides a nanodelivery platform with broad application potential and a new strategy for the regulation of IME.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Fansu Meng,Panpan Wang,Lina Yang,Junjie Yan,Anil K. Giri,Jaiwoo Lee,Kosuke Kusamori,Makiya Nishikawa,Shoko Itakura,Honghui Gu,Qi Li,Zhong Chen,Zhenjiang Yang,Detang Li,Yu Cai
Breast cancer (BC) stands as one of the primary malignancies threatening women's health. Baicalin (BA) exhibits anti - BC effects, yet it is plagued by issues such as low water solubility and poor bioavailability. To enhance the anti-BC efficacy of BA, this study developed a pH - responsive albumin nanoparticle loaded with BA (BA-AN-FA@mPEG). The optimized BA-AN-FA@mPEG features an average particle size of (185.8 ± 2.36) nm, a Zeta potential of (−32.70 ± 1.27) mV, an encapsulation efficiency of approximately 73.82 %, and a drug loading of about 5.68 %. The aqueous solution of BA-AN-FA@mPEG demonstrates excellent stability at 4 °C, with no significant drug leakage occurring within 12 weeks. BA-AN-FA@mPEG shows a certain sustained - release effect, and the drug release rate is faster under acidic conditions. The results of efficacy evaluation indicate that the half - maximal inhibitory concentration of BA-AN-FA@mPEG against MDA-MB-231 cells is 26.34 μg/mL. It can promote cellular uptake, effectively induce apoptosis, and cause cell cycle arrest. In summary, BA-AN-FA@mPEG is a promising tumor - targeted therapeutic formulation that can improve drug stability, tumor delivery efficiency, and therapeutic efficacy.
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ACTA PHYSICO-CHIMICA SINICA
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2025
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IF 13.5
Zihan Cheng,Kai Jiang,Jun Jiang,Henggang Wang,Hengwei Lin
Integrating stimuli-responsive luminescence with dynamic emission properties offers a powerful strategy to enhance information encryption through multi-level authentication systems. By rationally tuning the singlet-triplet energy gap (Δ E ST ) of a material, simultaneous activation of phosphorescence (Phos) and delayed fluorescence (DF) can be achieved, enabling programmable dynamic afterglow behavior. In this work, we report the first carbon dot (CD)-based thermoresponsive dynamic afterglow material, synthesized via in situ covalent immobilization of CDs within a cyanuric acid matrix. The resulting system demonstrates a thermally driven green-to-blue afterglow transition across a wide temperature range (273.15–423.15 K), exhibiting dual-mode thermochromic afterglow (TCA) and time-resolved afterglow (TRA) characteristics. Notably, a blue-to-green afterglow transition occurs above the threshold temperature of 348.15 K, where TRA dominates due to temperature-dependent exciton redistribution. This synergistic TCA-TRA interplay endows the material with unprecedented dynamic afterglow modulation capabilities. Structural and photophysical analyses confirm that covalent fixation reduces the Δ E ST of CDs from 0.46 to 0.28 eV, as designed. This ΔE ST engineering enables thermal control over the Phos/DF equilibrium, directly governing the observed dynamic emission. Finally, the potential applications of the prepared material in thermal monitoring and high-security information protection are also demonstrated.
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ACS Applied Nano Materials
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2025
·
IF 5.5
Mengxia Xu,Shengqian Wang,Chaochao Tao,Songsong Ding,Hualan Xu,Shengliang Zhong
Here, we report a synthesis method of Zn2SiO4@SiO2 hollow nanoparticles with adjustable thickness. A core–shell structured coordination polymer (CP) @ silica precursor was constructed by encapsulating silica on the surface of the CP through surface adsorption induced self-assembly. Then, the ideal shapes and large hollow cavities were produced through high-temperature calcination, and the formation process of hollow spheres was studied. Thanks to the hollow structure of the material, it has excellent drug loading capacity. It is worth noting that the thickness of the silica shell can be modulated by adjusting reagent amounts, calcination temperature, and time. Furthermore, this method exhibits broad applicability and can be extended to the synthesis of other hollow metal oxide materials. Persistent luminescence (PresL) was achieved through a simple ion-doping strategy. Notably, the silica shell thickness was found to regulate luminescence performance, and its underlying mechanism was further explored. This study not only establishes a synthetic route for designing hollow-structured PersL materials, but also offers perspectives on regulating the structure–performance relationships in multiphase composite materials. We hope the methodology developed and luminescent properties observed in this study will inspire the development of other innovative architectures integrating both physiological compatibility and theranostic capabilities.
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zhou Yang,Lingling Dai,Fangfei Liu,Kun Jia,Zhiyuan Ren,Yanmei Yang,Qingmeng Zhang,Weifeng Li,Ming Chen
Background Folate is an essential nutrient, but its bioavailability varies greatly depending on the molecular form present in food. The intake of forms that are not readily absorbed by the human body may lead to insufficient folate levels, particularly during pregnancy, thereby increasing the risk of birth defects. As a result, it is essential to develop a highly sensitive and accurate method capable of distinguishing between the two most common natural folates, 5-methyltetrahydrofolate (5-MTHF) and 5-formyltetrahydrofolate (5-FTHF), to ensure proper folate intake and health outcomes. Results In this study, a photochemical method was employed to load gold nanoparticles (Au NPs) onto carbon nanotubes (CNTs), successfully synthesizing CNTs@Au NPs nanocomposites that exhibit excellent thermal stability and robust surface-enhanced Raman scattering (SERS) activity. These nanocomposites, capable of maintaining structural integrity at temperatures as high as 400 °C, serve as highly effective substrates for in situ high-temperature SERS detection. Using this reliable high-temperature-assisted SERS approach, the structural changes of both folates at elevated temperatures were effectively monitored, enabling clear molecular fingerprinting and distinct differentiation between the two natural folates. Moreover, the CNTs@Au NPs demonstrate exceptional sensitivity, achieving a detection limit as low as 10 -8 M for both 5-MTHF and 5-FTHF, enabling accurate quantification of mixed folate samples and demonstrating reliable performance in real sample analysis. Significance The CNTs@Au NPs with in situ high-temperature SERS capabilities show great potential for various analytical applications. In particular, they provide an effective method for monitoring the quality of folate in dietary supplements and fortified foods, thus contributing significantly to the safety and quality assurance of folate products in the market.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
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IF 4.6
Min Sun,Xujie Wang,Lingwei Bu,Yawen Fan,Zihan Guo,Yingying Shan,Xiao Zhu,Lei Hu,Zhengquan Yan
Herein, a novel Bi 3+ -doped Au-Ag quantum dot (Bi 3+ -Au-Ag QD) system was synthesized, by homogeneous reduction method using AgNO 3 , Bi(NO 3 ) 3 ·5 H 2 O and HAuCl 4 ·4 H 2 O as raw materials, for multi-channel detection of S 2− in agricultural foods. Owing to the strong interaction between S 2− and the Ag/Au components, trace amounts of S 2− led to the formation of Ag 2 S or Au 2 S sulphides on the surface of the Bi 3+ -Au-Ag QDs. This surface modification altered the intrinsic fluorescence property of QDs, and their catalytic ability to the oxidization of colourless 3,3′,5,5′-tetramethylbenzidine (TMB) into its blue oxide (oxTMB), resulting in noticeable changes in spectral responses, colour and RGB values. Under optimised experimental conditions, Bi 3+ -Au-Ag QDs were employed successfully for ‘turn-on’ fluorescence, UV–vis and smartphone-assisted red-green-blue (RGB) detection of S 2− in real agricultural food samples. The limits of detection –4.75 × 10 −10 , 6.48 × 10 −9 and 7.22 × 10 −9 mol·L −1 respectively, well met the safety threshold set by the World Health Organization.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Shuangjun Li,Ruyue Shi,Yankun Liu,Mingcong Niu,Zhicun Liu,Zhongjuan Wang,Jiwang Gao,Hongda Cai,Zhuyu Zhou,Shengnan Li,Xiuhua Zhao
Immune cells infiltrating tumors play a pivotal role in tumor progression regulation; however, their functionality is frequently suppressed by the acidic conditions of the tumor microenvironment (TME). Targeting and reversing tumor acidity holds significant potential for enhancing the efficacy of immunotherapy. Here, we present a proof-of-concept study on tumor acidity-reversal therapy mediated by bacterial cellulose-targeted micelles to promote M2 macrophage repolarization to M1 macrophages and restore the anti-tumor function of T cells. Our strategy utilizes a bacterial cellulose (BC) composite modified with folic acid (FA) and chrysin (BY), loaded with lactic acid-neutralizing chemotherapeutic vincristine (VCR) and lactate dehydrogenase inhibitor GNE-140, to self-assemble into tumor-targeted nanoparticles. Post-treatment, FA-BC-BY@VCR@GNE-140 demonstrated notable efficacy in reversing tumor acidity, inducing tumor cell death, mitigating immunosuppression, and stimulating host adaptive immunity. This approach represents an innovative strategy for synergizing chemotherapy and immunotherapy to combat tumor progression.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Samuel Ariyo Okaiyeto,Shi-Han Yu,Haiou Wang,Jin-Hong Zhao,Cun-Shan Zhou,Ahmed Jamilu Mustapha,An-An Zhang,Fan Yang,Jing-Shou Zhang,Hong-Wei Xiao
This study evaluates vacuum-steam pulsed blanching (VSPB) as a pretreatment for Vigna unguiculata pods, focusing on drying kinetics, nutritional quality, and anti-nutritional factors. Pods underwent 0–4 VSPB cycles, followed by hot air drying at 50, 60, and 70 °C. VSPB reduced peroxidase activity to 4.8 %, enhancing enzyme inactivation. Texture analysis revealed an 85.6 % reduction in hardness, while the Midilli model provided the best description of drying kinetics (R 2 ≥ 0.9979). Specific energy consumption decreased by up to 40 % at 70 °C for blanched samples. VSPB preserved ascorbic acid, vitamin A, and chlorophyll, reduced lignin by 29.63 %, and lowered lectins from 71.01 to 8.88 Hu/mg. Folate retention declined with increased cycles. SEM and TEM analyses revealed microstructural and ultrastructural disruptions enhancing moisture diffusivity. VSPB proves effective in reducing anti-nutritional factors and retaining nutrients, offering a promising pretreatment for Vigna unguiculata with potential industrial applications.
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Foods
·
2025
·Vol. 14
·期 18
·3145
·
IF 5.1
Jing Li,Luyao Wang,Jijie Shi,Xuelian Wu,Jing Zhang,Yuecheng Xu,Xinhui Wang,Xiaoqin Li
Here, a highly sensitive and selective electrochemical sensor for dopamine (DA) determination in pork was developed. This sensor was fabricated using iron-based nanocomposites (Fe@(C-S-N)) to modify a glassy carbon electrode (GCE) substrate. The Fe@(C-S-N) nanocomposites were synthesized through a facile low-temperature chemical precipitation approach by adopting ferrous sulfate and melamine as raw materials, with their structural and compositional properties thoroughly investigated using multiple analytical techniques, including X-ray diffraction (XRD), electron microscopy (EM), X-ray photoelectron spectroscopy (XPS), and energy-dispersive spectra (EDS). Cyclic voltammetry (CV), chronocoulometry (CC), and differential pulse voltammetry (DPV) were adopted to investigate the electrochemical performance of the fabricated Fe@(C-S-N)/GCE sensor. Notably, the sensor demonstrated a linear response range from 0.05 to 100 μM with a minimal limit of detection (LOD) of 46 nM. Lastly, the fabrication sensor was used to determine DA in pork samples, showing an acceptable recovery of over 96.89%. Thus, the proposed sensor could offer an effective method for determining DA in pork.
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ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Hui Liu,Yi-Wei Liu,Ruo-Yu Yang,Mei-Juan Wu,Zi-Wei Yu,Jing-Wen Han,Chen-Zhi Zhang,Pin-Fang Huang,Ai-Lin Liu,Meng-Meng Liu
Background Methotrexate (MTX) is widely used in clinical practice for the treatment of malignant tumors and autoimmune diseases. High-dose MTX has been shown to be an effective approach for treating various malignant tumors, but it is accompanied by numerous toxic side effects, necessitating therapeutic drug monitoring (TDM) for patients and timely “folinic acid rescue.” High-performance liquid chromatography and fluorescent immunoassay (FIA) are currently used to detect MTX, but these methods are limited by complex sample preparation, time consumption, and high cost. Therefore, a simple, rapid, and cost-effective MTX measurement method is required. Results We developed a flexible and inexpensive electrochemical sensor using a stearyl trimethyl ammonium bromide (STAB)-modified, screen-printed carbon electrode to directly detect MTX in human serum. Assay performance was validated via detection of MTX in spiked buffer. The sensor was capable of measuring MTX concentrations ranging from 0.01–1 μM and 1–1500 μM, with a limit of detection of 3.1 nM and a limit of quantitation of 3.5 nM. For the samples simulating combined medication, the sensor exhibited outstanding selectivity, in cross-reactivity, with the maximum response value of interferents reaching only 3.49%. Additionally, the sensor shows reliable repeatability with a relative standard deviation of 3.8% and remarkable stability. Recovery in human serum validated the clinical utility of the sensor in point-of-care testing conditions. The sensor’s applicability to personal medicine was confirmed by detecting MTX blood concentration in patients with different diseases. The results obtained by the sensor were compared with those obtained by the FIA technique, a method commonly used in hospitals, showing a high level of consistency between both methods. Significance To meet the requirements of personalized medicine for MTX-patients, we developed a disposable biosensor with wide detection range from 0.01–1 μM and 1–1500 μM. Owing to the effective enrichment of STAB, the electrochemical response was sensitive, selective, stable, and rapid. As per the clinical test results, our sensor has shown a high level of consistency with the FIA method, indicating its potential to replace FIA as a cost-effective platform for MTX-TDM.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2025
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IF 21.1
Jiaojiao Sun,Mengxia Shen,A.jun Chang,Jianfeng Huang,Pengbo Wang,Yonghao Ni
Conventional metal-nitrogen-carbon single-atom catalysts (M-N-C SACs) are plagued by electron localization effects arising from symmetric coordination geometries and the absence of synergistic interactions among isolated active sites, both of which critically undermine oxygen reduction reaction (ORR) catalytic performance. To address these limitations, this study introduces a multiscale synergistic engineering strategy that integrates asymmetric coordination design with bioinspired structural innovation. Specifically, the symmetry of the Fe-N 4 coordination is disrupted to generate Fe-N 3 Se sites, which facilitate charge density redistribution; while Fe atomic clusters (FeACs) are strategically integrated to construct asymmetric catalytic centers that activate O 2 and promote O-O bond cleavage. A hierarchical porous carbon submicronspheres architecture, inspired by alveolar structures and featuring gradient-distributed micropores and mesopores, has been developed to enhance the mass transport efficiency at triple-phase interface. Overall, we have developed FeSAs and FeACs anchored on alveolar-inspired hierarchically porous nitrogen/selenium co-doped carbon submicronspheres (Fe SA+AC -N/Se-AHCs), which exhibits outstanding ORR activity (E o =0.99 V, E 1/2 =0.90 V) and long-term durability. Theoretical analyses further demonstrate that the electronic coupling between Fe-N 3 Se sites and FeAC synergistically optimizes the adsorption free energies of critical *OOH and *O intermediates, thereby accelerating O-O bond dissociation. This work presents a comprehensive cathode catalyst platform for developing high-energy-density and long-cycle-life Zinc-air batteries.
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