Bioresource Technology Reports
·
2018
·
IF 0
Bing-Qian Wang,Bo Peng,Jian-Fei Sun,Chu Wang,Ke Jiang,Jing-Hao Ma,Peng-Qi Zhang,Yu-Cai He
An effecti v e combination pretreatment of bamboo shoot shell (BSS) using Emulsifier OP-10 (0.5%, w/v) in the acidified media (pH 4.0) by autoclaving (120 °C, 40 min) was conducted. After BSS slurry without detoxification was enzymatically hydrolyzed for 96 h, glucose (25.2 g/L) and xylose (7.5 g/L) were obtained from 50 g/L BSS at high yields. HPLC results showed that no potential inhibitors (furfural and 5-hydroxymethylfurfural) formed. In the hydrolysates , an effective biocatalytic synthesis of ethyl ( S )-4-chloro-3-hydroxybutanoate (100% yield; >99% e.e. ) from 1000 mM ethyl 4-chloro-3-oxobutanoate was developed without the extra addition of cofactors within 24 h. In summary, this combination strategy involving biomass pretreatment, enzymatic saccharification , and bioreduction can be developed for producing chiral alcohols.
研究领域:
生物质预处理
·
酶解糖化
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生物催化
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手性醇合成
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DOI
MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·4
·
IF 5.3
Shui Huaqin,Guo Yanli,Geng Jingbo,Chang Qing,Tian Yuxin,Du Xue,Jin Yan,Li Baoxin,Liu Wei
Volatile sulfur compounds (VSCs) in human exhaled breath (EB) are considered as biomarkers of halitosis. Convenient and reliable detection of VSCs has significance for confirming oral health. Herein, copper-based metal-organic framework (Cu-TPPE) was synthesized by utilizing aggregation-induced emission (AIE) phenomenon of tetrakis(4-pyridylphenyl)ethylene (TPPE) ligand on paper. Due to electron transfer between Cu 2+ and TPPE, the aggregation-induced emission of TPPE was quenched. In the presence of VSCs, the affinity between S and Cu led to delocalization of Cu in Cu-TPPE, thus recovering the aggregation-induced chemiluminescence emission between TPPE and bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H 2 O 2 . Based on this principle, selective and sensitive determination of VSCs in human EB was achieved. The linear range of quantification of H 2 S was 0.07-8.0 ppm, and the detection limit was 0.03 ppm. This paper-based chemiluminescence platform has been applied to the detection of VSCs in real breath samples. Graphical
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 23
·5653-5661
·
IF 3.637
Xie Wei,Zhang Jian,Zeng Yanbo,Wang Hailong,Yang Yiwen,Zhai Yunyun,Miao Dongwei,Li Lei
A novel molecularly imprinted fluorescent sensor for the determination of 4-nitroaniline (4-NA) was synthesized via free radical polymerization with 3-[(7-methoxy-2-oxo-2 H -chromen-4-yl)methyl]-1-vinyl-1 H -imidazol-3-ium bromide as the fluorescence functional monomer, 4-NA as the template molecule, ethylene glycol dimethacrylate as the cross-linker, and 2,2′-azo(bisisobutyronitrile) as the initiator. The obtained fluorescent poly(ionic liquid) was characterized through Fourier transform infrared, scanning electron microscopy, Brunauer–Emmett–Teller analysis, and fluorescence spectrophotometry. The fluorescent sensor had high fluorescence intensity, short detection time (0.5 min), good selectivity, and excellent sensitivity (limit of detection = 0.8 nM) for 4-NA, with good linear relationships of 2.67–10,000 nM. The practical applicability of the fluorescence sensor in detecting 4-NA in industrial wastewater and spiked environmental water was demonstrated, and a satisfactory result was obtained.
Highly sensitive and selective detection of 4-nitroaniline in water by a novel fluorescent sensor based on molecularly imprinted poly(ionic liquid)
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JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Kexin Hou,Fengli Chen,Yuangang Zu,Lei Yang
An efficient method for the extraction of vitexin , vitexin-4″- O -glucoside, and vitexin-2″- O -rhamnoside from Phyllostachys edulis leaves comprises heat treatment using an ionic liquid–lithium salt mixture (using 1-butyl-3-methylimidazolium bromide as the solvent and lithium chloride as the additive), followed by ultrasound-assisted extraction. To obtain higher extraction yields, the effects of the relevant experimental parameters (including heat treatment temperature and time, relative amounts of 1-butyl-3-methylimidazolium bromide and lithium chloride, power and time of the ultrasound irradiation, and the liquid–solid ratio) are evaluated and response surface methodology is used to optimize the significant factors. The morphologies of the treated and untreated P. edulis leaves are studied by scanning electron microscopy. The improved extraction method proposed provides high extraction yield, good repeatability and precision, and has wide potential applications in the analysis of plant samples.
研究领域:
植物化学
·
分析化学
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PubMed
高影响因子
DIAMOND AND RELATED MATERIALS
·
2021
·
IF 3.315
Wei Jiang,Pinghua Chen,Xueqin Li,Guanghui Wu,Huitao Zheng,Chenxuan Li,Lei Fang,Hualin Jiang
Electrochemical analysis using specifically designed electrodes represents a promising strategy to detect organic pollutants, such as 4-nitrophenol (4-NP), in the environment. Towards this goal, a metal-free composite of phenanthroline doped graphitic carbon nitride (g-C 3 N 4 -PD) was synthesized, characterized, and applied as the electrode materials for electrochemical detection of 4-NP in water. The phenanthroline doping simultaneously extends the π-conjugation system of g-C 3 N 4 , and creates defect sites in g-C 3 N 4 , leading to better conductivity, higher 4-NP adsorption, and significantly enhanced electrocatalytic activity . Electrochemical sensors were fabricated by using g-C 3 N 4 -PD-modified carbon electrode. They showed a broad detection concentration range of 2.854–1117 μM and a low detection limit of 39 nM for 4-NP, with high reproducibility, stability and selectivity . Reliable and accurate measurements of 4-NP in real-life water samples have been demonstrated using these electrodes.
研究领域:
环境科学
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材料科学
·
分析化学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jiaohui Xia,Yan Li,Xinbai Jiang,Dan Chen,Jinyou Shen
Electricity-stimulated anaerobic system (ESAS) has shown great potential for halogenated organic pollutants removal. Exogenous redox mediators can improve electron transfer efficiency to enhance pollutants removal in ESAS. In this study, humic acid (HA), a low-cost electron mediator, was added into ESAS to enhance the simultaneous reductive debromination and mineralization of 4-bromophenol (4-BP). Results showed that the highest 4-BP removal efficiency at 48 h was 95.43 % with HA dosage of 30 mg/L at − 700 mV, which was 34.67 % higher than that without HA. The addition of HA decreased the requirement for electron donors and enriched Petrimonas and Rhodococcus for humus respiratory. HA addition regulated microbial interactions, and enhanced species cooperation between Petrimonas and dehalogenation species ( Thauera and Desulfovibrio ), phenol degradation-related species ( Rhodococcus ) as well as fermentative species ( Desulfobulbus ). Functional genes related to 4-BP degradation ( dhaA/hemE/xylC/chnB/dmpN ) and electron transfer ( etfB/nuoA/qor/ccoN/coxA ) were increased in abundance by HA addition. The enhanced microbial functions, as well as species cooperation and facilitation, all contributed to the improved 4-BP biodegradation in HA-added ESAS. This study provided a deep insight into microbial mechanism driven by HA and offered a promising strategy for improving halogenated organic pollutants removal from wastewater.
研究领域:
环境工程
·
微生物学
·
生物修复
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Xingxin Wang,Dongshu Du,Haibin Dong,Sunfengda Song,Kwangnak Koh,Hongxia Chen
Nanoparticle (NP) structure, compositing and the nature of the NP-functionalized electrode interface have a strong influence upon electrochemical properties that are critical in applications such as sensing, photocatalysis and electrocatalysis . Existing methods to fabricate NP-functionalized electrodes do not allow or precise control over all these variables, especially the NP-electrode interface, making it difficult to understand and predict how structural changes influence electrode activity and consequently limit the application. To conquer this problem, in this study, we fabricated a stepwise construction of a novel supramolecular stabilized gold nanoparticles (AuNPs) multilayer mediated by guest molecules, yielding 3D AuNPs assembly at the electrode interface. para -Sulfonatocalix[4]arene (pSC 4 ), a water soluble macrocyclic synthetic receptor, has been served as a stabilizing ligand for preparation and gaining new insights into pSC 4 stabilized gold nanoparticles (pSC 4 -AuNPs) tethered on the electrode interface through host-guest interaction. We investigated the electrochemical properties of multilayer pSC 4 -AuNPs modified gold electrode using different core size of AuNPs with varying layer number. The electron transfer ability was characterized by electrochemical impedance spectroscopy (EIS). Electrochemical signals are significantly enhanced through the layer-by-layer assembly of pSC 4 -AuNPs due to its high conductivity and high effective area. With this innovative method, by taking the assay of a tumor marker as an example, human epidermal growth factor receptor 2 (ErbB2) was successfully measured with a detection limit of 0.5 ng/mL. Taking the advantage of the pSC 4 -AuNPs multilayer's good biocompatibility , high effective area and high electronic transmission, 3D AuNPs multilayer produced on the electrode interface suggests a portable synthetic pathway for the application into sensitive electrochemical biosensor .
研究领域:
纳米技术
·
电化学
·
生物传感器
·
材料科学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiang Pu,Jiahua Zhang,Jinwei He,Zhihui Ai,Xiaoxue He,Xiaojun Zhou,Shiyuan Tong,Xinyue Dai,Qiqi Wu,Jiayu Hu,Jingshu He,Hanguang Wang,Wei Wang,Jinqiu Liao,Li Zhang
The biosynthetic route for flavonol in Camptotheca acuminata has been recently elucidated from a chemical point of view. However, the genes involved in flavonol methylation remain unclear. It is a critical step for fully uncovering the flavonol metabolism in this ancient plant. In this study, the multi-omics resource of this plant was utilized to perform flavonol O -methyltransferase-oriented mining and screening. Two genes, CaFOMT1 and CaFOMT2 are identified, and their recombinant CaFOMT proteins are purified to homogeneity. CaFOMT1 exhibits strict substrate and catalytic position specificity for quercetin, and selectively methylates only the 4′-OH group. CaFOMT2 possesses sequential O -methyltransferase activity for the 4′-OH and 7-OH of quercetin. These CaFOMT genes are enriched in the leaf and root tissues. The catalytic dyad and critical substrate-binding sites of the CaFOMTs are determined by molecular docking and further verified through site-mutation experiments. PHE181 and MET185 are designated as the critical sites for flavonol substrate selectivity. Genomic environment analysis indicates that CaFOMT s evolved independently and that their ancestral genes are different from that of the known Ca10OMT . This study provides molecular insights into the substrate-binding pockets of two new CaFOMTs responsible for flavonol metabolism in C. acuminata .
研究领域:
植物生物化学
·
分子生物学
·
植物化学
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PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Xingjie Yang,Xubao Jiang,Muhammad Sohail Bashir,Xiang Zheng Kong
Polyurethanes (PUNs) have been widely used in many fields. However, there are few reports on their microsphere preparation through precipitation polymerization. Herein, uniform PUN microspheres are prepared by precipitation polymerization of toluene diisocyanate and trimethylolpropane, without any surfactant and stabilizer. Under optimized conditions, monomers are fully polymerized within 2 h at a monomer concentration as high as 25.0 wt %, with a high yield of the microspheres (96.9%). By loading Pd on their surface, composite microspheres Pd@PUN are obtained and used as catalysts. 4-Nitrophenol is completely reduced within 1 min, with a kinetic constant of 4.96 min–1, significantly higher than many reported Pd-loaded catalysts, and with comparable reusability. Used in degradation of dye molecules, the catalytic performance is practically unchanged after 20 recycled uses. This work provides a simple method for the preparation of highly uniform PUN microspheres with a high yield and their Pd-loaded catalysts, both of great potential for various applications.
研究领域:
材料科学
·
催化化学
·
环境科学
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高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2021
·Vol. 222
·期 23
·2100284
·
IF 2.527
Da Zhang,Jinxin Zheng,Pengfei Zhang,Ronghui Zhao,Zhuo Chen,Meng Wang,Kuilin Deng
In the synthesis of traditional AIE-active polymers with conjugate or multi-benzene system, the troublesome process is still faced such as consuming time, high temperature, catalysis, no water or vacuum, and so on. In this study, the polydihydropyrimidone derivatives (PDPMDs) with AIE properties are facilely synthesized by the introduction of 4-dihydropyrimidone rings onto non-AIE-active intermediate polyureas (PUs) in Biginelli reaction. It is found that the unsymmetrical L- lysine ethyl diisocyanate and butylenediamine played a very important role in the smooth preparation and fluorescence enhancement of PDPMDs. Compared with the intermediate PUs, the fluorescence of PDPMDs is significantly enhanced (35-fold increase), which is mainly due to the intramolecular aggregation of dihydropyrimidone rings on PUs chains. At the same time, with the increase of the content of dihydropyrimidone rings, the fluorescence intensity of PDPMDs also increases, and the emission wavelength shows an obvious red shift. The fluorescence of PDPMDs also showed obvious dependence on excitation wavelength, which further reveals the clusterization-triggering emission (CTE) mechanism. In summary, the strategy of endowing AIE properties to non-AIE active polymer by introducing heterocycles into the backbone through multi-component reaction provides useful guidance for AIE functionalization of other non-AIE materials.
研究领域:
材料科学
·
化学
·
高分子科学
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DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Chendong Yang,Entao Li,Xiaoping Guo,Wenyu Xie,Yuanzhan Wang,Xuefei Huang,Sandra Chiu,Xuanjun Wu
Over the past 3 years, SARS-CoV-2 Omicron has been circulating globally with the emergence of multiple subvariants, including BA.5, BA.5.2, XBB, XBB.1, EG.5.1, HK.3, BA.2.86, JN.1, and KP.2. To combat these Omicron subvariants, several vaccines based on receptor-binding domain (RBD) dimers have been developed; however, RBD dimer vaccines require frequent updates to cope with the emergence of new variants. In contrast, the development of a safe, effective, and broad-spectrum vaccine against multiple Omicron subvariants, including the latest JN.1 and KP.2, would be a one-size-fits-all solution. Here, we designed BA.4/5 RBD-PC7A conjugate micelles by displaying the BA.4/5 RBD in PC7A micelles. Remarkably, the micelles elicited potent neutralizing antibodies (NAbs) in rabbits, effectively neutralizing BA.5.2, XBB.1.18, and HK.3 infections. Moreover, the micelles alone were able to induce NAbs in mice against the BA.5 variant. When a cytosine-phosphate-guanine (CpG) adjuvant was added and electrostatically adsorbed to the micelles, there was a significant increase in the antibody titers of IgG1, IgG2b, and IgG2c. This enhancement facilitated the broad neutralization of various strains, including BA.5.2, XBB.1.18, HK.3, JN.1, and KP.2. Furthermore, the micelles adsorbed with CpG protected golden hamsters from infection with the BA.5.2 strain. This study presents a potent and broadly neutralizing nanovaccine that includes the BA.4/5 RBD antigen and a CpG adjuvant. It demonstrates efficacy against multiple Omicron subvariants, including BA.5, BA.5.2, XBB.1.18, HK.3, JN.1, and KP.2, highlighting its potential for clinical translation.
研究领域:
免疫学
·
病毒学
·
疫苗学
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PubMed
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 9
·2088
·
IF 4.2
Yingchao Sun,Yuxin Chai,Shuangming Li,Yongcheng Chen,Chunhui Ma,Rongzheng Huang,Fanfan Zhang
Background: Nettle is a potential non-conventional feed resource due to its high level of crude protein content, and ensiling is better for utilization in the animal industry. Previous integrated analysis (microbiome and metabolome) suggested that 4-pentenoic acid and malic acid in ensiled nettle may inhibit harmful microorganisms within the system. The present study investigated the effects of these two acids on nettle silage quality through the addition of 1% fresh weight of these acids, then analyzed the characteristics and bacterial communities during 60 days of nettle ensiling. Results: The addition of 4-pentenoic acid increased the content of crude protein (CP) and acetic acid (AA) compared with both control and malic acid-treated groups during 30 to 60 days of ensiling (p< 0.05). Lactic acid (LA) content was highest in the malic acid-treated group (4.21%, dry matter, DM based) compared to the control and 4-pentenoic acid-treated groups after 7 days of nettle ensiling (p< 0.05), but lower compared with the 4-pentenoic acid-treated group after 30 days of nettle ensiling (p< 0.05), and it was not detected in all groups after 60 days of silage. The contents of butyric acid (BA) and ammonia (AN) were the lowest (2.92–4.39% of DM and 9.94–24.28% of total nitrogen, respectively) in the 4-pentenoic-treated group compared with both control and malic acid-treated groups during 30 to 60 days of ensiling (p< 0.05). Both acids increased the relative abundance ofWeissellaafter 30 days of nettle ensiling, with 4-pentenoic acid showing a higher inhibitory capacity. Both acids showed a trend to inhibit the relative abundance ofClostridium sensu stricto 15after 30 days of nettle ensiling.Clostridium sensu stricto 15showed significant positive correlation with BA and AN (p< 0.05). Conclusions: The results of the present study suggested that the addition of 4-pentenoic could improve the quality of silage by reducing levels of protein degradation, probably resulting from its inhibited activity againstClostridiumspp. However, malic acid was less effective than 4-pentenoic acid in suppressingClostridiumspp. activity and the associated production of BA and AN, resulting in inferior preservation of CP.
研究领域:
动物科学
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微生物学
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植物科学
·
生物化学
·
生物技术
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高影响因子
危险品化学品经营许可证（带存储）