Science of Advanced Materials
·
2022
·Vol. 14
·期 8
·1410-1418
·
IF 1.067
Xin Jia-Ying,Li Yue,Liu Feng-Yuan,Sun Li-Rui,Wang Yan,Xia Chun-Gu
Cu(II) adulteration in preserved vegetables by unreliable producers to increase their visual appeal or compensate for natural colour changes is most common. Methanobactin (Mb) is a Cu(II)-coordinated peptide, which plays crucial role in Cu(II) capture and absorption and the catalysis
of Cu(II)-containing methane monooxygenase in methanotrophs. In this study, Mb-functionalized gold nanoparticles (Mb-AuNPs) was applied to develop a dual readout colorimetric detection for Cu(II) adulteration in preserved vegetables. In the presence of Cu(II), Mb-AuNPs was assembled into nanocluster
driven by Cu(II)/Mb coordination, and the assembly process was monitored by the visible spectrum on the variation of surface plasmon resonance peak. The results showed that, Mb-AuNPs possessed excellent peroxidase-like activity after Cu(II) coordination. In the presence of Mb-AuNPs, the amount
of Cu(II) obviously affected the rate of hydroquinone oxidation by H2O2 and the change of A246. The visible and ultraviolet dual-readout detection system containing Mb-AuNPs, hydroquinone and H2O2 exhibited high sensitivity and selectivity
for Cu(II). The linear range of visible and UV detection towards Cu(II) was 0.05–0.4 μmol/L, the limit of detection were 37.50 nmol/L and 4.48 nmol/L, respectively. In conclusion, the Cu(II) coordination driven Mb-AuNPs assembly makes it able to detect Cu(II) adulteration in
preserved vegetables by dual channel methods to eliminate the occurrence of false positives and improve the accuracy of measurement results. Such non-spatially separated Mb-AuNPs assembly and it catalyzed hydroquinone oxidation dual channel readout was simple, fast, reliable and significantly
facilitated the detection efficiency of Cu(II).
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高影响因子
Journal of Hazardous Materials Advances
·
2023
·
IF 0
Abdul Haleem,Shengqi Chen,Jianming Pan,He Weidong
γ − radiation is a clean, fast and efficient synthetic route for polymeric materials. it is very powerful tool to generate free radicals during polymerization without any need of initiator and accelerator in both hydrophilic and hydrophobic media. Here in this work, for the first time, we fabricated acrylamide-methyl-propane sulfonic acid based-single and double network hydrophilic cryogels via γ − radiation at low pH. pH highly effects the synthesis of acrylamide-methyl-propane sulfonic acid based cryogels using redox initiation method. Along with pH, the effect of radiation time and radiation dose rate on cryo-polymerization was also studied. Gold hybrid cryogels (single and double network) were fabricated via self-reduction method at ambient conditions. Fourier transform infrared spectroscopy was implemented for chemical structure analysis of the synthesized material. The surface morphology of single and double network cryogels was observed via scanning electron microscopy. For the hybrid cryogels materials analysis x-ray diffraction, thermal gravimetric analysis, energy dispersive x-ray analysis, x-ray photoelectron spectroscopy and transmission electron microscopy and was used to confirm the presence of nanoparticles in the polymer network. The fabricated hybrid cryogels (0.15 g) were used for the degradation of Congo red (50 mL) as model dye in the presence of NaBH 4 (0.02 g) with efficient and fast degradation activity. The apparent rate constant value (K app ) for single and double network hybrid cryogels was 0.3652 min −1 and 0.2268 min −1 , respectively. The activation energy, enthalpy and entropy was calculated (34.44 kJ.mol −1 , –30.85 kJ.mol −1 and –146.30 J·mol −1 k −1 , respectively) using Arrhenius and Eyring equations for the fabricated catalyst. The catalyst was tasted in the tap water system and its efficiency was satisfied in real world too. The fabricated double wall hybrid catalyst will have a significant importance in the environmental field.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Zhengfang Liang,Shuwei Xiao,Junhong Li,Guoli Zuo,Jihua Wei,Yuanxun Gong,Qianli Tang,Kai Zhang,Xianjiu Liao
Apurinic/apyrimidinic endonuclease 1 (APE1), a key enzyme in the base excision repair pathway, is frequently overexpressed in multiple cancers and has emerged as an important biomarker for early diagnosis and therapeutic response evaluation. In this work, a highly sensitive electrochemiluminescence (ECL) biosensor was developed for APE1 detection by integrating a PAM-regulated CRISPR-Cas12a activation mechanism with a multifunctional nanocomposite-modified electrode. A Ti₃C₂/AuNPs/CsPbBr₃ ternary nanocomposite was fabricated through ultrasonic assembly to provide enhanced conductivity, water stability, and probe-loading capacity. Meanwhile, a DNA duplex containing an AP site within the PAM region was engineered to regulate Cas12a activation through APE1-mediated cleavage and polymerase-assisted repair, thereby minimizing background interference and enabling highly specific signal generation. Ferrocene-labeled DNA tetrahedrons (TDNs) were further introduced as a spatially ordered probe scaffold to improve hybridization efficiency and facilitate Cas12a trans-cleavage–mediated signal release. This work presents three key innovations: (1) Construction of a Ti₃C₂/AuNPs/CsPbBr₃ nanocomposite that simultaneously enhances ECL stability, conductivity, and electron-transfer efficiency, addressing the inherent water-instability of perovskite emitters. (2) Coupling of a PAM-regulated APE1–KF repair mechanism with Cas12a activation to achieve highly specific AP-site recognition and selective signal generation. (3) Introduction of a TDN-based probe architecture that provides rigid spatial orientation and high-density probe loading, markedly improving hybridization efficiency and Cas12a trans-cleavage performance. Benefiting from these synergistic innovations, the biosensor achieves a detection limit of 6.28 × 10⁻⁸ U μL⁻¹ and a wide linear range (1 × 10⁻⁷ to 1 × 10⁻ 1 U μL⁻¹). The platform also demonstrates excellent specificity, reproducibility, and reliable performance in diluted serum, highlighting its promise for clinical and biochemical applications.
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高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 38
·4503-4514
·
IF 2.896
Yasan He,Xiaogeng Lin,Yuan Tang,Ling Ye
In this study, an electrochemical sensing strategy was developed based on the synergies of gold nanoparticles (AuNPs) doped carboxylated covalent organic frameworks (ACOFs) and poly(fuchsin basic) film for the simultaneous detection of ascorbic acid (AA), dopamine (DA), and uric acid (UA). This strategy not only took advantage of the adopted materials but also made use of the H-bonding and electrostatic interaction between the three compounds and materials. For this sensing, a poly-BFu film was formed on the surface of bare glass carbon electrode (GCE) under a constant potential. AuNPs was highly dispersed and immobilized on the constructed ACOF-TaTp to obtain AuNPs@ACOF. The constructed sensor AuNPs@ACOF/p-BFu/GCE combined the merits of high surface area, hydrophilicity, conductivity, and selective affinity, consequently exhibiting high sensitivity and selectivity toward the simultaneous detection of AA, DA, and UA with wide linear response ranges of 25–1500 μM, 0.75–40 μM, and 1–200 μM, respectively. The corresponding detection limits were 12.0 μM, 0.15 μM, and 0.22 μM. The simultaneous determination of UA in real human urine sample further confirmed the practicability of the designed electrode.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Xiaoqi Chen,Aiyou Huang,Peng Ju
The integration of sterilization capabilities into sensing platforms is a crucial step in mitigating the significant health risks associated with the persistence of pathogenic bacteria. In this study, we developed a photoelectrochemical (PEC) pathogenic bacteria sensing platform integrated with sterilization function. The platform was based on the g-C 3 N 4 nanosheets decorated with AuNPs and Poly(3-thiophene boronic acid) (PTBA). The incorporation of AuNPs and PTBA proved highly beneficial for enhancing the PEC and photocatalytic performance of bulk g-C 3 N 4 . Leveraging the boronic acid group of PTBA, we achieved the selective capture of pathogenic bacteria on the surface of the electrode. The platform showed a high detection sensitivity towards Escherichia coli ( E. coli ) with a low limit detection (30.5 cfu/mL). Furthermore, the sensing platform demonstrated an exceptional antibacterial rate (99.9 %) resulting from the photoelectrocatalytic effect of PTBA/AuNPs/g-C 3 N 4 . This research presents a potential application of the platform as a self-cleaning biosensor for pathogenic diagnosis.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Chao Zhang,Fengnian Zhao,Yongxin She,Sihui Hong,Xiaolin Cao,Lufei Zheng,Shanshan Wang,Tengfei Li,Mengqiang Wang,Maojun Jin,Fen Jin,Hua Shao,Jing Wang
A novel electrochemical sensor based on molecularly imprinted polymeric membrane as biomimetic recognition element was fabricated by electropolymerization of o -aminothiophenol (O-ATP) on gold nanoparticle/electrochemical reduction graphene oxide-modified screen-printed carbon electrode (AuNPs/ERGO-SPCE) for sensitive and selective detection of cyhexatin (CYT) pesticides. The sensor was constructed through self-assembly of O-ATP and CYT, following by the electropolymerization of O-ATP in the presence of CYT based on the hydrogen-bonding interaction. The obtained imprinted polymer film was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), fourier transforms infrared (FT-IR) spectra, cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal condition, both the obtained imprinted sensors based on AuNPs/ERGO/SPCE presented satisfactory linearity with the certain concentrations of CYT and the limit of detection (LOD) can reach 0.20 ng mL −1 . The modification of ERGO and AuNPs could generate synergistic effect to increase the sensitivity of the MIP sensor. The developed imprinted sensors exhibited excellent long-term stability and repeatability. Furthermore, the established method was successfully applied to the determination of CYT in pear samples.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yongkang Ye,Wuwen Yan,Yaqian Liu,Shudong He,Xiaodong Cao,Xuan Xu,Haisong Zheng,Sundaram Gunasekaran
A rapid and sensitive electrochemical biosensor was constructed to detect Salmonella using invA gene biosensor. The biosensing was based on polyrrole-reduced graphene oxide (PPy-rGO) nanocomposite modified glassy carbon electrode (GCE) and signal amplification with horseradish peroxidase-streptavidin biofunctionalized gold nanoparticles (AuNPs-HRP-SA). PPy-rGO was prepared at 60 °C by chemical reduction of PPy-functionalized graphene oxide (PPy-GO) that was synthesized by in situ polymerization at room temperature . The detection signal was amplified via enzymatic reduction of H 2 O 2 in the presence of hydroquinone (HQ) using AuNPs-HRP-SA as nanotag. Under optimal conditions, the differential pulse voltametric (DPV) signal from the biosensor was linearly related to the logarithm of target invA gene concentrations from 1.0 × 10 −16 to 1.0 × 10 −10 M, and the limit of detection (LOD) was 4.7 × 10 −17 M. The biosensor can also detect Salmonella in the range of 9.6 to 9.6 × 10 4 CFU mL −1 , with LOD of 8.07 CFU mL −1 . The biosensor showed good regeneration ability , acceptable selectivity , repeatability and stability, which bode well as an alternative method for Salmonella screening.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Minghui Wang,Huanshun Yin,Yunlei Zhou,Xiangjian Meng,Geoffrey I.N. Waterhouse,Shiyun Ai
Herein, a novel photoelectrochemical (PEC) biosensor was developed for the ultrasensitive detection of dual microRNAs (miRNAs), with the detection being based on energy transfer (ET) between carbon quantum dots (CQDs) and gold nanoparticles (AuNPs). The PEC platform consisted of a [email protected] 2 C nanotube modified ITO electrode. Two hairpin probes (H1 and H2) carrying the Au NPs were used “switch off” and “switch on” the PEC signal of the CQDs, with a close approach of the tagged AuNPs to the CQDs quenching the PEC signal. The introduction of different miRNAs (miRNA-159b and miRNA-166a) altered the interparticle distance between the AuNPs and CQDs, thereby affecting the intensity of the PEC response. This approach allowed the highly sensitive detection of both miRNA-159b and miRNA-166a. The linear range of the biosensor for miRNA-159b and miRNA-166a detection were 0.5–5000 fM, with low detection limits of 0.15 fM and 0.21 fM, respectively. To our knowledge, this is the first reported CQDs-based ET biosensor for the PEC detection of dual miRNAs. Results suggest that this approach offers a promising platform for the ultrasensitive detection of multiple miRNAs.
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Biao Jia,Baoshan He,Renli Liu,Jinping Yang,Wenjie Ren,Zhiguang Suo,Yiwei Xu
The conductivity of Zn-MOF-on-Co-MOF synthesized by one-pot method is improved by searching for the optimum carbonization temperature, which overcomes the limitation of traditional MOF. In order to further enhance electron transfer, the mesoporous PtPdCo trimetal was introduced, which provided considerable load capacity for methylene blue (MB) and reverse complementary DNA (sDNA), and also showed excellent catalytic activity for MB. In this study,the conductivity of aptasensor was improved by modifying carbonized MOF as the base material. In the presence of fipronil (FIP), Apt tended to bind to FIP and was separated from the electrode surface, so that the remaining single-stranded complementary DNA (cDNA) were hybridized with signal probe (MB@PtPdCo/sDNA) and amplified the electrochemical signal. By modifying the substrate, competitive amplification strategy and signal probe material to catalyze signal molecules, the sensor performs multiple signal amplification, and the electrical signal is significantly improved. The sensor has a wide detection range 100 pg/mL to 100 μg/mL and a low detection limit of 76 pg/mL.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yujun Zhong,Zhiyang Li,Anlin Zhang,Yuqing Peng,Hao Zhou,Yaping Guo,Danqing Lu,Lianwu Xie,Shuyun Shi
A novel electrochemical sensor MIP/AuNPs/GCE was developed by depositing gold nanoparticles and electropolymerizing molecularly imprinted film on the surface of glassy carbon electrode (GCE) for highly sensitive detection of neutral phosmet residues in fruits and vegetables. Scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy, cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the imprinted sensor. The results showed that the imprinted film on GCE was evenly prepared with better specific recognition of phosmet on MIP/AuNPs/GCE than that on bare GCE. Several parameters including deposition time, functional monomer and its molar ratio, scanning cycles, and elution time were optimized in detail. The linear range of the MIP/AuNPs/GCE prepared in this work was 0.020–4.0 nmol/L with limit of detection of 0.010 nmol/L, which was much lower than MIP/GCE (0.17 nmol/L). The identification mechanism of MIP/AuNPs/GCE for phosmet was also confirmed as adsorption-diffusion model with effective surface area increasing after mediated by AuNPs. Selectivity, reproducibility and repeatability of the MIP/AuNPs/GCE were acceptable. The recovery of applying the sensor to detect PM in real samples ranged from 91.40 % to 107.3 %, which agreed well with those of HPLC. The method of developing MIP/AuNPs/GCE sensor herein could be extended to detecting other neutral pesticide residues in food and environment.
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高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Yangqing He,Na Xu,Lucas Binnah Junior,Xing Hao,Binghua Yao,Qian Yang,Dongjie Liu,Zhanying Ma
Construction of semiconductor-assisted noble metal nanocomposites has been approved as an efficient strategy to achieve promising photocatalysts. In this paper, highly efficient AuNPs/h-BN nanocomposites have been fabricated by loading gold nanoparticles (AuNPs) as interfacial electron transfer mediators onto the hexagonal boron nitride nanosheets (h-BN). The SEM and HRTEM images, together with XPS analysis revealed that AuNPs were successfully deposited onto the surface of h-BN and the layered heterojunctions were generated between AuNPs and h-BN. The optimum 15%AuNPs/h-BN nanocomposites exhibit dramatically enhanced photocatalytic performance for degradation of levofloxacin hydrochloride with 84.4% removal rate, and hydrogen evolution of 61.15 μmol within 5 h. It is almost 4 and 61 times higher than that of pure h-BN, respectively. In addition, this is the first time using h-BN based material to evaluate the elimination efficiency of levofloxacin hydrochloride. The corresponding enhanced mechanism for 15%AuNPs/h-BN can be attributed to the effective interfacial electron-hole pairs separation and migration between h-BH and AuNPs and its surface Plasmon absorption (SPA). Moreover, the possible decomposition pathways for levofloxacin hydrochloride are proposed according to the UPLC-MS analysis. Herein, we have provided a good example for removing antibiotic residues by using h-BN-based metal nanocomposites towards environmental remediation.
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高影响因子
ANALYTICAL LETTERS
·
2026
·
IF 1.8
Jiahui Liu,Yan Xu,Tongtong Ran,Zhilei Li,Jianping Zhou
Indole-3-acetic acid (IAA) is an important plant hormone and its rapid determination is useful for evaluating plant growth and physiological status. In this work, a screen-printed carbon electrode (SPCE) modified with a composite of MXene, gold nanoparticles (AuNPs), and reduced graphene oxide/carbon nanofibers (rGO-CNFs) incorporating an electrospun carbon-nanofiber network was developed for the voltammetric determination of IAA. The composite was characterized by morphological, structural, and chemical analyses. The modified electrode showed a larger electrochemically active area and lower charge-transfer resistance than the bare SPCE. Under optimized conditions, least-squares regression over 0.05–125 µM based on six concentration levels gave a slope of 1.060 ± 0.016 µA µM−1, a limit of detection (LOD) of 12.1 nM, and a limit of quantification (LOQ) of 40.3 nM. In buffered model solutions, the electrode showed acceptable selectivity, fabrication repeatability, and short-term electrochemical stability. These results support the MXene-AuNPs/rGO-CNFs-modified SPCE as a low-cost platform for IAA determination, while validation in real plant matrices remains necessary.
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高影响因子
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