Advanced Healthcare Materials
·
2021
·Vol. 11
·期 3
·2101633
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IF 9.933
Shi Wenjie,An Jinxia,Tan He,Jia Mengran,Gao Hui
Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) remain a matter of concern, as the clinical use of multiple antibiotics induces antibiotic resistance in bacteria, resulting in the failure of treatments. Despite the emergence of anti-adhesion strategies that can prevent the development of bacterial drug resistance, these strategies are mainly used for disease prevention rather than effective treatment. Photothermal therapy (PTT) has emerged as an efficient alternative for the elimination of bacteria. Nevertheless, high local temperatures related to PTT probably cause damage to surrounding healthy tissue. Herein, a biomimetic nonantibiotic nanoplatform for low-temperature photothermal treatment of UTIs is developed. The nanoplatform comprises polydopamine (PDA) photothermal core and biphenyl mannoside (Man) shell with multivalent high-affinity to UPEC. Scanning electron microscope (SEM) shows PDA-Man possessed ultra-strong targeting binding ability toward UPEC. It is the fact that this impulse UPEC to form a large bacterial cluster. Consequently, the high photothermal energy of the PDA-Man appears predominantly in the affected bacterial area, while the overall environment remains at a low temperature. The fabricated nanoplatform shows excellent photothermal bactericidal effects, approximately 100% in a UTI model. Overall, this low-temperature photothermal nanoplatform provides an appropriate strategy for the elimination of bacteria in clinical applications.
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FOOD MICROBIOLOGY
·
2021
·
IF 5.516
Shilin Wu,Xueming Xu,Na Yang,Yamei Jin,Zhengyu Jin,Zhengjun Xie
Non-conventional heating technology based on electric fields can be utilized to process liquid foods . In this study, the induced electric field (IEF) was investigated to clarify its inactivation mechanism on E.coli . Staining results show that inactivation of E.coli by IEF can be attributed to the reversible destruction of the cell membrane, followed by the denaturation of intracellular enzymes, and finally the irreversible rupture of the cell membrane. The increased levels of extracellular proteins and nucleic acids were also observed. IEF treatment at 400 Hz and 800 V (or 53 V/cm) results in a reduction of 4.5 log CFU·mL −1 in the number of E.coli . Storage life analysis shows that IEF treatment can improve the stability of apple juice and the content of bioactive components. Thus, IEF is a potential technique for liquid food processing.
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BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Yu Zhang,Minhua Wei,Guihong Zhao,Wenjie Zhang,Yingzi Li,Beibei Lin,Yanjun Li,Qingyang Xu,Ning Chen,Chenglin Zhang
l -Homoserine is a valuable non-proteinogenic amino acid used in the synthesis of various important compounds. Microbial fermentation has potential value for producing l -homoserine on a large scale, but suffers from a low yield and the need for expensive additives. In this study, a non-induced, non-auxotrophic, plasmid-free Escherichia coli chassis for the high-efficiency production of l -homoserine was constructed. Initially, the l -homoserine degradation pathway was dynamically attenuated. Subsequently, systems metabolic engineering strategies were employed, including reinforcing the synthetic flux, improving NADPH generation, and elevating l -homoserine efflux. The constructed strain HOM-14, produced 60.1 g/L l -homoserine without additional supplements or inducers, which achieved the highest fermentative production efficiency of l -homoserine till date. Moreover, common byproducts, such as acetate, did not accumulate. The strategies presented here can be applied in the further engineering of chassis for the scale-up production of l -homoserine and derivatives.
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JOURNAL OF DAIRY SCIENCE
·
2020
·
IF 3.333
Tao Yang,Xinyan Yang,Xiaojie Guo,Shiqian Fu,Jiapeng Zheng,Sihan Chen,Xue Qin,Zhenghui Wang,Dongyan Zhang,Chaoxin Man,Yujun Jiang
ABSTRACT Escherichia coli O157:H7 is an extremely serious foodborne pathogen accounting for a vast number of hospitalizations. In this system, a simple, rapid, and safe compound method was developed based on carbonyl iron powder (CIP) and multiwalled carbon nanotubes (MWCNT). Then, the [email protected] aptasensor was constructed by strong π-stacking between nanocomposite and aptamer, single-strand DNA, causing fluorescent quenching of the dye-labeled aptamer. The restoration of dye fluorescence could be achieved when aptamer came off the surface of the [email protected] nanocomposite due to the presence of target bacteria. To the best of our knowledge, this fabrication of magnetic carbon nanotubes without irritating and corrosive reagents is described for the first time. The sensing platform was also an improvement on the conventional formation of the aptasensor between carbon materials and DNA aptamer. The nanocomposite was verified by diverse characterization of zeta potential, Fourier-transform infrared spectroscopy, transmission electron microscopy, and energy dispersive x-ray analysis. The [email protected] aptasensor was an effective nanoplatform for quantitative detection of E. coli O157:H7, and was measured to have high specificity, good reproducibility, and strong stability. The aptasensor's capacity to quantify E. coli O157:H7 was as low as 7.15 × 10 3 cfu/mL in pure culture. The detection limit of E. coli O157:H7 was 3.15 × 10 2 cfu/mL in contaminated milk after 1 h of pre-incubation. Hence, the developed assay is a new possibility for effective synthesis of nanocomposites and sensitive tests of foodborne pathogens in the dairy industry.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Jinmei Fu,Yaofeng Zhou,Xiaolin Huang,Wenjing Zhang,Yuhao Wu,Hao Fang,Cunzheng Zhang,Yonghua Xiong
The conventional colloidal gold immunochromatographic assay (AuNP-ICA) cannot meet the requirements for the rapid and sensitive detection of Escherichia coli (E. coli) O157:H7 because of its poor sensitivity. Herein, a novel two-step cascade signal amplification strategy that integrates in situ gold growth and nanozyme-mediated catalytic deposition was proposed to enhance the detection sensitivity of conventional AuNP-ICA dramatically. The enhanced strip displayed ultrahigh sensitivity in E. coli O157:H7 detection and had a detection limit of 1.25 × 101 CFU/mL, which is approximately 400-fold lower than that of traditional AuNP-ICA (5 × 103 CFU/mL). The amplified strip had no background signal in the T-line zone in the absence of E. coli O157:H7 even after one round of cascade signal amplification. The enhanced strip demonstrated excellent selectivity against E. coli O157:H7 with a negligible cross-reaction to nine other common pathogens. Intra-assays and interassays showed that the improved strip has acceptable accuracy and precision for determining E. coli O157:H7. The average recoveries in a real milk sample ranged from 87.33 to 112.15%, and the coefficients of variation were less than 10%. The enhanced strip also showed great potential in detecting a single E. coli O157:H7 cell in a 75 μL solution.
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FEMS MICROBIOLOGY LETTERS
·
2019
·Vol. 366
·期 24
·
IF 1.994
Ge Yan,Guo Senlin,Liu Tao,Zhao Chen,Li Duanhua,Liu Yangchang,Li Jinjun,Liang Tao,Wang Lu
A nuclease from Yersinia enterocolitica subsp. palearctica (Nucyep) is a newly found thermostable nonspecific nuclease. The heat-resisting ability of this nuclease would be extremely useful in biological research or pharmaceutical production. However, the application of this nuclease is limited because of its poor yield. This research aimed to improve Nucyep productivity by producing a novel genetically engineered Escherichia coli and optimizing the production procedures. After 4 h of induction by lactose, the new genetically engineered E. coli can express a substantial amount of Nucyep in the form of inclusion bodies. The yield was approximately 0.3 g of inclusion bodies in 1 g of bacterial pellets. The inclusion bodies were extracted by sonication and solubilized in an 8 M urea buffer. Protein renaturation was successfully achieved by dilution method. Pure enzyme was obtained after subjecting the protein solution to anion exchange. The Nucyep showed its nonspecific and heat resistant properties as previously reported (Boissinot et al. 2016). Through a quantification method, its activity was determined to be 1.3 × 10 6 Kunitz units (K.U.)/mg. These results can serve as a reference for increasing Nucyep production.
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ANALYTICAL CHEMISTRY
·
2023
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IF 7.4
Ying Wang,Tong Bu,Yuanyuan Cao,Haiyu Wu,Jia Xi,Qinlin Feng,Chenyu Xuan,Li Wang
Nanozymes have drawn much attention as an enzyme mimetic with low cost and stability in enhancing analytical performance. Herein, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed employing the bimetallic PdRu nanozyme to replace the natural enzymes as a catalytic carrier for the sensing of Escherichia coli O157:H7 (E. coli O157:H7). The PdRu nanozyme displayed ultrahigh catalytic activity, possessing a catalytic rate that was 5-fold higher than horseradish peroxidase (HRP). In addition, PdRu exhibited great biological affinity with antibody (affinity constant was about 6.75 × 1012 M) and high stability. All those advantages ensure the successful establishment and the construction of a novel colorimetric biosensor for E. coli O157:H7 detection. PdRu-based ELISA not only achieved an ultrasensitive detection sensitivity (8.7 × 102 CFU/mL) by approximately 288-fold as compared to the traditional HRP-based ELISA and also possessed satisfactory specificity and reproducibility (relative standard deviation (RSD) < 10%). Furthermore, the feasibility of PdRu-ELISA was further evaluated by detecting E. coli O157:H7 in actual samples with satisfactory recoveries, indicating its potential for applications in bioassays and clinical diagnostics.
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Microbial Biotechnology
·
2023
·
IF 5.7
Xinyue Ye,Jian Wang,Pengfei Xu,Xiaoqian Yang,Qixue Shi,Genyan Liu,Zhaoshi Bai,Changlin Zhou,Lingman Ma
Polymyxin resistance is conferred by MCR-1 (mobile colistin resistance 1)-induced lipopolysaccharide (LPS) modification of G − bacteria. However, the peptide MSI-1 exerts potent antimicrobial activity against mcr-1 -carrying bacteria . To further investigate the potential role of MCR-1 in improving bacterial virulence and facilitating immune evasion, and the immunomodulatory effect of peptide MSI-1, we first explored outer membrane vesicle (OMV) alterations of mcr-1 -carrying bacteria in the presence and absence of sub-MIC MSI-1, and host immune activation during bacterial infection and OMV stimulation. Our results demonstrated that LPS remodelling induced by MCR-1 negatively affected OMV formation and protein cargo by E. coli . In addition, MCR-1 diminished LPS-stimulated pyroptosis but facilitated mitochondrial dysfunction, further aggravating apoptosis in macrophages induced by OMVs of E. coli . Similarly, TLR4-mediated NF-κB activation was markedly alleviated once LPS was modified by MCR-1. However, peptide MSI-1 at the sub-MIC level inhibited the expression of MCR-1, further partly rescuing OMV alteration and attenuation of immune responses in the presence of MCR-1 during both infection and OMV stimulation, which can be exploited for anti-infective therapy.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 8
·7649
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IF 5.6
Yanjing Liu,Xiyue Li,Yuezhou Liu,Yaping Huang,Fuming Wang,Yongfang Qian,Ying Wang
Due to their high porosity, large specific surface area, and structural similarity with the extracellular matrix (ECM), electrospun nanofiber membranes are often endowed with the antibacterial properties for biomedical applications. The purpose of this study was to synthesize nano-structured Sc2O3-MgO by doping Sc3+, calcining at 600 °C, and then loading it onto the PCL/PVP substrates with electrospinning technology with the aim of developing new efficient antibacterial nanofiber membranes for tissue engineering. A scanning electron microscope (SEM) and energy dispersive X-ray spectrometer (EDS) were used to study the morphology of all formulations and analyze the types and contents of the elements, and an X-ray diffraction (XRD), thermogravimetric analysis (TGA), and Fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR) were used for further analysis. The experimental results showed that the PCL/PVP (SMCV-2.0) nanofibers loaded with 2.0 wt% Sc2O3-MgO were smooth and homogeneous with an average diameter of 252.6 nm; the antibacterial test indicated that a low load concentration of 2.0 wt% Sc2O3-MgO in PCL/PVP (SMCV-2.0) showed a 100% antibacterial rate againstEscherichia coli(E. coli).Keywords:nano-textured;Sc2O3-MgO;antibacterial;electrospinning
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JOURNAL OF BIOTECHNOLOGY
·
2023
·
IF 4.1
Fang-Ying Zhu,Qing Yang,Min Cao,Ken Zheng,Xiao-Jian Zhang,Qi Shen,Xue Cai,Zhi-Qiang Liu,Yu-Guo Zheng
d -(−)-Pantolactone (DPL) is a key intermediate for the production of d -(+)-pantothenate (vitamin B5). Deracemization of d , l -pantolactone (D, L -PL) through oxidizing l -(+)-pantolactone (LPL) to ketopantoyl lactone (KPL) and subsequently reducing KPL to DPL is a promising route for synthesizing DPL. Herein, a newly mined l -pantolactone dehydrogenase from Rhodococcus hoagie ( Rho LPLDH) was used for the oxidative dehydrogenation of LPL. To alleviate inclusion bodies formed by membrane-bound Rho LPLDH intracellular expression in E. coli , strategies involving chaperone assistance and decreasing induction temperature were used to achieve Rho LPLDH soluble expression. To enhance its activity, directed evolution and hydrophilicity-based engineering yielded increased catalytic activity and thermostability . 1 M LPL was efficiently converted to KPL by engineering strain CM5 co-expressing Rho LPLDH L254I/V241I/I156L/F224Q/N164K and chaperone. A “two stages in one-pot” method was employed in deracemization of 1 M D, L -PL with 91.2% yield. These results demonstrated that CM5 catalyst exhibits great potential in enzyme cascade deracemization for the production of DPL.
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ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Jinxi Feng,Yao Fu,Xiaosheng Liu,Shuanghong Tian,Shenyu Lan,Ya Xiong
Piezoelectric effect was first used for disinfection. In this process, hydrothermally synthesized nano/micrometer tetragonal-BaTiO3 was selected as the piezo-catalyst which was stressed by ultrasound to generate piezoelectric potential. It was found that the inactivation number of the nano/micrometer tetragonal BaTiO3 exposure or sonication alone within 180 min was 0.30 log and 0.70 log for E. coli in deionized water, respectively, while addition of 2 g tetragonal-BaTiO3 with piezo-activity in the ultrasound system resulted in a 2.72 log inactivation without the input of any additional energy, only by original ultrasound. The latter was 2.72 folds as great as the sum of the two former inactivation numbers. However, addition of cubic-BaTiO3 without piezo-activity only led to a 1.02 log inactivation. And moreover, in the process of serious physical damage and chemical oxidation of cells, substantial leakages of intracellular materials were observed. These great differences not only clearly indicated that the combination of sonication and piezocatalysis possessed a great inactivation potential but also suggested that there was a significant synergistic inactivation effect between piezocatalytic oxidation and sono-mechanical destruction.
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Nanomaterials
·
2018
·Vol. 8
·期 4
·234
·
IF 3.504
Xian Xia,Zijie Zhou,Shijuan Wu,Dan Wang,Shixue Zheng,Gejiao Wang
Selenite reductase CsrF overexpressedEscherichia coliwas used as a microbial factory to produce Se(0) nanoparticles (Bio-SeNPs). The Bio-SeNPs were characterized by transmission electronic microscopy, element mapping, scanning electron microscopy, energy-dispersive X-ray spectrographs, Zeta-potential, dynamic light scattering, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses. The results indicated that Bio-SeNPs are irregular spheres with diameters from 60 to105 nm and mainly consist of Se(0), proteins and lipids. Furthermore, it exhibited maximum adsorption capacity for anionic dye (congo red) at acidic pH and cationic dyes (safranine T and methylene blue) at alkaline pH. To gain more insight, adsorption kinetics, adsorption isotherms and adsorption thermodynamics studies were carried out. These results showed that the adsorption capacities of congo red, safranine T and methylene blue were 1577.7, 1911.0 and 1792.2 mg/g, respectively. These adsorption processes were spontaneous and primarily physical reactions. In addition, Bio-SeNPs can be effectively reused by 200 mmol/L NaCl. To the best of our knowledge, this is the first report of adsorption removal dyes by Bio-SeNPs. The adsorption capacities of Bio-SeNPs for congo red, safranine T and methylene blue were 6.8%, 25.2% and 49.0% higher than that for traditional bio-based materials, respectively.Keywords:CsrF;Bio-SeNPs;anionic dye;cationic dye;adsorption
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危险品化学品经营许可证（带存储）