TALANTA
·
2020
·
IF 5.339
Shimeles Addisu Kitte,Terefe Tafese,Chen Xu,Muhammad Saqib,Haijuan Li,Yongdong Jin
The development of highly sensitive electrochemiluminescence (ECL) immunosensors by using functional nanoparticles as signal amplifiers is a solution towards sensitive determination of many low concentration disease biomarkers. Herein, a sensitive aptamer-based, sandwich-type surface plasmon enhanced electrochemiluminescence (SPEECL) immunosensor was demonstrated for the detection of cardiac troponin I (cTnI), by means of aptamer conjugated CdS QDs and AuNPs as ECL luminophores and plasmon sources, respectively, in which Tro4 aptamer was used as a capture probe for cTnI and Tro6 aptamer as a detecting probe. The signal of the developed SPEECL system showed ~ 5-fold increment as compared to that of without AuNPs. Using this ECL platform for the detection of cTnI, a linear range and the limit of detection (LOD) were found to be 1 fg/mL - 10 ng/mL and 0.75 fg/mL, respectively.
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2020
·
IF 4.225
Zhang Jiaqi,Liang Xiaoqiang,Li Jiacheng,Yin Hao,Liu Fangchen,Hu Cheng,Li Ling
Overuse of acetaminophen (APAP) is a major cause of drug-induced liver failure at the clinics. Apigenin (API) is a natural flavonoid derived from Matricaria chamomilla. The aim of the present study was to investigate the amelioration function of API in APAP-induced hepatotoxicity both in vitro and in vivo and investigate its potential mechanisms. Analysis results of the activities of serum alanine and aspartate aminotransferases (ALT and AST), malondialdehyde, myeloperoxidase (MPO), and reactive oxygen species (ROS) demonstrated therapeutic effects of API. MTT assay results revealed that API attenuated APAP and its metabolic product, N-acetyl-p-benzoquinone imine (NAPQI) induced cytotoxicity in a dose-dependent manner in human liver cells, L-02 cells. Subsequently, metabolomic results of cells and serum analyses demonstrated an aberrant level of carnitine palmitoyltransferase I (CPT1A). We established that API stimulated CPT1A activity in mice liver tissues and L-02 cells. Molecular docking analyses revealed potential interaction of API with CPT1A. Further investigation of the role of CPT1A in L0-2 cells revealed that API reversed cytotoxicity via the AMP-activated protein kinase (AMPK)/GSK-3β signaling pathway and compound C, which is a selective AMPK inhibitor, inhibited activation of CPT1A induced by API. API was bound to the catalytic region of AMPK as indicated by molecular docking results. In addition, compound C suppressed nuclear translocation of nuclear factor erythroid 2–related factor 2 (NRF2) that is enhanced by API and inhibited the antioxidative function of API. In summary, the study demonstrates that API attenuates APAP-induced hepatotoxicity by activating the AMPK/GSK-3β signaling pathway, which subsequently promotes CPT1A activity and activates the NRF2 antioxidant pathway.
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DOI
PubMed
高影响因子
Bioactive Carbohydrates and Dietary Fibre
·
2021
·
IF 0
Dongmei Wu,Xiaoliang Zheng,Weiwei Hu,Kai Zhu,Chengxiao Yu,Qiaojun He,Robert J. Linhardt,Xingqian Ye,Shiguo Chen
Pectic oligosaccharides are viewed as excellent immunomodulator, but the specific molecular weight (M w ) range required for anti-inflammatory effects remains unclear. In this study, citrus pectins was depolymerized by the ultrasound/H2O2/Vc system and the pectic mixtures was separated through the superdex 30 chromatography column . Three highly purified pectin fractions (USP1, USP2, USP3) were obtained, with the M w of 2713 g/mol, 3683 g/mol, and 7469 g/mol. All of them were RG-I pectins and USP3 contained more arabinan sidechains than USP1 and USP2. In vitro anti-inflammation assays based on LPS-induced RAW264.7 macrophages indicated that these pectins could suppress the NO release, ROS production and down-regulated the level of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in a M w -dependent manner. The inhibitory effects increased in the order of USP13KD should be effective as a prophylactic agent against inflammatory diseases in functional food.
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DOI
TALANTA
·
2024
·
IF 5.6
Sai-Nan Qin,Yun-Chuan Nong,Chuan-Liang Cao,Li-Yang Chen,Yi-Jie Cao,Ting Wan,Lei Feng,Kalle Salminen,Jian-Jun Sun,Ju Li
Acute myocardial infarction (AMI) is one of the top contributors to global disease mortality. AMI biomarkers, such as cardiac troponin I (cTnI), are often detected with enzyme-linked immunosorbent assay (ELISA) that suffers from several well-known drawbacks such as poor stability and slow and cumbersome operation. Therefore, it is necessary to develop a new analytical technique that can rapidly analyse and detect cTnI for early screening of AMI. In this work, a nanoporous electrochemical aptamer-based (E-AB) sensor for rapid and sensitivite detect of cTnI was designed. Firstly, the aptamer was truncated, and then molecular docking simulation and circular dichroism (CD) were used to screen for aptamers with significant conformational changes when binding to the target, in order to enhance the sensitivity of E-AB sensors. Subsequently, nanoporous electrodes with active area 20 times higher than that of smooth electrodes were fabricated by electrochemical alloying/dealloying, which enabled E-AB sensors to obtain higher signal-to-noise ratios, providing favorable assurance for the detection results. Under optimal conditions, E-AB sensors could specifically detect cTnI in serum and blood with a detection limit of 1 pg/mL. At the same time, the sensor and enzyme-linked immunoassay (ELISA) had identical detection results when measuring target levels from real clinical samples. Furthermore, the sensor exhibited good reproducibility, stability, providing a simple and low-cost method for detecting cTnI, which is expected to help early AMI patients obtain accurate diagnosis.
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DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Shuhong Song,Lei Wang,Yaqian Qu,Huimin Li,Hongshuai Wang,Peizhuo Han,Zeliang Gao,Xutang Tao
The crystal growth mechanism of form I (AZ-I) of the antihypertensive drug azilsartan is revealed by in situ atomic force microscopy (AFM). On the dominant (100) face, AZ-I grows by a classical spiral growth mechanism. The growth spiral is asymmetric due to the different step kinetics in different crystallographic directions. The normal growth rate of the (100) face is inferred to be much lower than the step velocity within this face according to the step geometry, resulting in the plate-like crystal morphology. The dependence of step velocity on terrace width indicates that molecules incorporate into steps primarily by a surface diffusion mechanism involving molecular adsorption on terraces at first and then diffusion into steps. Within the (100) face, steps in different directions exhibit different step edge free energies and total minimal energies for step advancement. The step kinetics depends on the step length within a certain range. These findings provide a fundamental understanding of drug crystal growth at the microscale.
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DOI
高影响因子
Solar RRL
·
2024
·Vol. 8
·期 7
·2400088
·
IF 6
Lili Gao,Ke Hao,Ping Hu,Sheng Huang,Fan Yang,Deng Li,Shengzhong (Frank) Liu
Halide perovskites have gained significant attention in the field of photovoltaics and photocatalysis due to their exceptional optoelectronic properties. However, the exploration of other classes of 2D perovskite materials is limited. In this work, the photocatalytic performance of (HA)PbX 4 2D crystals embedded with MoS 2 in their bulk structure is investigated. The (HA)PbX 4 @MoS 2 composites are directly synthesized in a (HA)PbX 4 crystal solution. Notably, the (HA)PbI 4 @MoS 2 composite exhibits a remarkable 2.94-fold increase in H 2 evolution activity. Furthermore, the composite demonstrated stability in a (HA)PbX 4 (HA = histamine, X = I, Br, Cl)-saturated aqueous HX solution. The photogenerated electrons in (HA)PbX 4 are efficiently transferred to the MoS 2 sites, facilitating the reduction of protons to H 2 . In this study, the potential of hybrid 2D perovskite–MoS 2 composites is highlighted as promising visible-light photocatalysts for aqueous solution applications.
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DOI
高影响因子
Journal of Food Measurement and Characterization
·
2023
·Vol. 18
·期 1
·95-104
·
IF 3.4
Sun Xi,Liu Feng,Xue Xiao
Ensuring food safety necessitates rapid and reliable food contaminant detection methods. Here, we present a novel approach that integrates electrochemical sensors with machine learning to detect Sudan Red I in food. Our study showcases a modified stainless steel needle (SSN) electrode adorned with silver nanoparticles (AgNPs) and investigates three distinct convolutional neural network (CNN) architectures: Inception V1, ResNet-50, and SqueezeNet V1.1. This combination aims to predict the sensor response accurately. The AgNPs/SSN electrode displays heightened electrochemical activity towards Sudan Red I, offering a linear detection range of 0.1–20 μM and an exceptional 10 nM detection limit. Notably, the Inception V1 and ResNet-50 networks exhibit superior predictive capability compared to SqueezeNet V1.1. Our interdisciplinary approach establishes a rapid, sensitive, and dependable detection system for food contaminants. By fusing electrochemical sensors with machine learning, we propel the development of intelligent detection systems with significant implications for food safety enhancement. Graphical abstract
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Jing Wu,Jiaqi Huang,Xiaowei Chi,Jianhua Yang,Yu Liu
Aqueous Zn–Mn2+ electrolysis batteries utilizing the two-electron-transfer reaction between Mn2+ and MnO2 attract great attention because of their superior theoretical capacity (616 mAh g–1). However, the low conductivity of deposited MnO2 and the poor conversion efficiency of Mn2+/MnO2 inevitably result in limited areal capacity and unsatisfied cycling stability, which have become the main hurdles of aqueous Zn–Mn2+ batteries’ applications. Herein, we propose a novel Mn2+/I– hybrid cathode that couples the triiodide/iodide redox with the Mn2+/MnO2 redox to optimize the electrolysis kinetics. Because of the synergistically enhanced conversion reaction between Mn2+/I– and the promoter effect of I– to the dissolution of MnO2, this hybrid cathode not only exhibits fast reaction kinetics, thus demonstrating ultrahigh rate capability (100 mA cm–2), but also displays observably enhanced conversion efficiency up to 96.0% with excellent reversibility of 2000 cycles. Especially the superhigh areal capacity of 20 mAh cm–2 with more than 100 cycles among the reported static Zn–Mn2+ electrolysis batteries is demonstrated. The excellent battery performance as well as the facile electrode hybridization approach designed here paves the way for the practical applications of aqueous Zn batteries.
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DOI
高影响因子
Inorganic Chemistry Frontiers
·
2022
·Vol. 9
·期 18
·4602-4607
·
IF 7.779
Meng Zhao,Shengqiang Liu,Hao Cai,Fangyi Zhao,Zhen Song,Quanlin Liu
Near-infrared (NIR) light devices have great potential in non-destructive testing and biomedicine fields. However, the lack of stable and efficient long wavelength NIR phosphors hinders their development. In this work, utilizing the compositional flexibility of antimonate double perovskites, we realized efficient and tunable broadband NIR emissions based on A2MSbO6:Cr3+ materials (A = Ca, Sr, and Ba; M = Ga, In, Sc, and Y). The NIR emission peaks ranged from 825 to 1010 nm by a composition modulation. In particular, Ca2ScSbO6:Cr3+ exhibits an 850 nm-centering broadband NIR emission with an internal quantum efficiency of 84.5% and a high thermal quenching temperature, retaining ∼71% at 410 K of the initial intensity at 80 K. Comparatively, Sr2InSbO6:Cr3+ with a 920 nm-centering broadband emission has 63.2% internal quantum efficiency. This work could help in discovering novel NIR phosphors with long-wavelength emissions and high efficiency.
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DOI
高影响因子
Journal of the American Chemical Society
·
2020
·
IF 14.612
Xianju Zhang,Xianxin Wu,Xiaoyu Liu,Gaoyu Chen,Yongkai Wang,Jianchun Bao,Xiangxing Xu,Xinfeng Liu,Qi Zhang,Kehan Yu,Wei Wei,Jingjing Liu,Jun Xu,Hua Jiang,Peng Wang,Xun Wang
Perovskite and chalcogenide quantum dots (QDs) are important nano semiconductors. It has been a challenge to synthesize heterostructural QDs combining perovskite and chalcogenide with tailorable photoelectronic properties. In this report, heterostructural CsPbX3-PbS (X = Cl, Br, I) QDs were successfully synthesized via a room temperature in situ transformation route. The CsPbX3-PbS QDs show a tunable dual emission feature with the visible and near-infrared (NIR) photoluminescence (PL) corresponding to CsPbX3 and PbS, respectively. Typically, the formation and evolution of the heterostructural CsPbBr3–PbS QDs with reaction time was investigated. Femtosecond transient absorption spectroscopy (TAS) was applied to illuminate the exciton dynamics in CsPbBr3–PbS QDs. The mild synthetic method and TAS proved perovskite to PbS energy transfer may pave the way toward highly efficient QD photovoltaic and optoelectronic devices.
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DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2025
·
IF 3.7
Zheng Liu,Jun-Jie Fang,Zhi-Yi Wang,Yun-Peng Xie,Xing Lu
Graphical The inclusion of carboxylic acid ligands significantly impacts the structural arrangement of the metal core in Cu(I) alkynyl clusters, thereby affecting their fluorescence properties. Furthermore, the incorporation of ferrocene into these clusters has been noted to induce modifications in the electrochemical performance of the Cu(I) alkynyl clusters. Surface ligands play a pivotal role in the functional design of atomically precise Cu(I) nanoclusters. They act as protective agents, ensuring the stability of the Cu(I) clusters, while also influencing their structure and properties. This study delves into the synthesis, structure, and properties of innovative Cu(I) nanoclusters co-stabilized by carboxylic acid and alkynyl ligands. The findings reveal that these surface ligands wield a significant impact on the cluster's structure and can even modulate the luminescent characteristics of the Cu(I) alkynyl clusters. Moreover, density-functional theory (DFT) calculations shed light on how different carboxylic acid ligands affect the UV-visible absorptivity of paired Cu(I) clusters. In addition, ferrocene carboxylic acids were employed as protective ligands to impart electrochemical properties to the Cu(I) clusters. This research presents an effective methodology for synthesizing atomically precise Cu(I) clusters shielded by carboxylic acid ligands.
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产品关联
DOI
PubMed
高影响因子
International Journal of Clinical and Experimental Pathology
·
2015
·
IF 1.891
Qingwei Zhang,Haiya Wu,Congcong Wen,Fa Sun,Xuezhi Yang,Lufeng Hu
MGCD0103, an isotype-selective HDACi, has been clinically evaluated for the treatment of hematologic malignancies and advanced solid tumors, alone and in combination with standard-of-care agents. In this study, we developed a serum metabolomic method based on gas chromatography-mass spectrometry (GC-MS) to evaluate the effect of intragastric administration of MGCD0103 on rats. The MGCD0103 group rats were given 20, 40, 80 mg/kg of MGCD0103 by intragastric administration each day for 7 days. Pattern recognition analysis, including both principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) revealed that intragastric administration of MGCD0103 induced metabolic perturbations. As compared to the control group, the levels of L-alanine, L-isoleucine, and L-leucine of MGCD0103 group decreased. The results indicate that metabolomic methods based on GC-MS may be useful to elucidate side effect of MGCD0103 through the exploration of biomarkers (L-alanine, L-isoleucine, and L-leucine). According to the pathological changes of liver at difference dosage, MGCD0103 is hepatotoxic and its toxity is dose-dependent.
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DOI
PubMed
高影响因子
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