SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Wenjuan Dong,Ruiping Wang,Xiaojuan Gong,Wenting Liang,Chuan Dong
A novel far-red fluorescence resonance energy transfer (FRET) fluorescent probe for ratiometric detection of l -cysteine ( l -Cys) has been designed. The system was established a FRET assembly by positively charged carbon dots (CDs) and negatively charged N -acetyl- l -cysteine capped gold nanoparticles (NAC-AuNPs). The fluorescence of CDs at 539 nm could be effectively quenched in the presence of NAC-AuNPs owing to FRET process, while the emission of NAC-AuNPs at 630 nm was appeared. Subsequently, the interactions between l -Cys and NAC-AuNPs resulted in the decreased emission intensity of NAC-AuNPs, but the emission intensity of CDs kept almost constant due to the continuous FRET efficiency. The ratio of emission intensities at 539 and 630 nm ( I 539 / I 630 ) exhibited a linear correlation to the l -Cys concentration in the range of 1.0–110 μM with the detection limit of 0.16 μM. Moreover, this far-red ratiometric sensor also revealed excellent selectivity toward l -Cys over other amino acids , which showed very high potential in the practical application for diagnosing of cysteine-related disease.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Qi Zhang,Mingxuan Fu,Haijun Lu,Xinyu Fan,Haiyang Wang,Yufan Zhang,Huan Wang
Novel chiral amino acids biosensor based on both potential difference and current ratio simultaneously was first established by employing left/right-handed double helix carbon nanotubes (L/D-DHCNT) with chiral structure as substrate material, and by self-assembly anchoring L/D-cysteine (L/D-Cys) on the surface of the left/right-handed double helix carbon nanotubes@Polypyrrole@Au nanoparticles nanocomposites (L/D-DHCNT@PPy@AuNPs). In this work, L/D-DHCNT@PPy@AuNPs@L/D-Cys was successfully constructed via a facile and environment-friendly process. Interestingly, in order to obtain highly dispersed AuNPs deposited on L/D-DHCNT, polypyrrole polymerized from pyrrole acts as a locating agent. Owing to the combination of unique performance of the L/D-Cys and L/D-DHCNT with chiral structure, and PPy and AuNPs with outstanding conductivity, an extremely efficient electrochemical chiral biosensing system was constructed that exhibited enhanced conductivity, sensitivity and chiral recognition capacity for tyrosine (Tyr), tryptophan (Trp) and glutamic acids (Glu) enantiomers. Utilizing both the potential difference and current ratio signal to commendably realize the electrochemical recognition and quantitative determination of amino acid enantiomers in their racemic solution. In addition, achieving the detection of Tyr in tablet samples by fabricated ultra-efficient electrochemical chiral biosensing system. Therefore, L/D-DHCNT@PPy@AuNPs@L/D-Cys as novel and ultrasensitive chiral biosensing system possesses the capability of chiral recognition of amino acid enantiomers, and it opens up the potential application of L/D-DHCNT@PPy@AuNPs@L/D-Cys in the field of chiral biosensors.
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Food Analytical Methods
·
2018
·Vol. 11
·期 9
·2438-2446
·
IF 2.245
Niu Jiahua,Zhang Xiaoting,Qin Peige,Yang Yixin,Tian Shufang,Yang Hui,Lu Minghua
In this research, a dispersive solid-phase extraction (dSPE) with graphene oxide@SiO 2 (GO@SiO 2 ) nanocomposites as the adsorbent followed by high-performance liquid chromatography analysis was developed for simultaneous determination of melatonin, l -tryptophan, and two l -tryptophan-derived esters in food (black sesame seed ( Sesamum indicum L. ) was selected in this case). The GO@SiO 2 nanocomposite was prepared by one-pot aggregation in aqueous phase with sol-gel technique. The structure and morphology of the GO@SiO 2 were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform-infrared spectroscopy, and X-ray diffraction. The extraction conditions of dSPE including the ratio of material to liquid, adsorption and desorption time, desorption temperature, and desorption solvents were investigated, respectively. The detection limits of the developed method for the analysis of melatonin, l -tryptophan, l -tryptophan methyl ester, and l -tryptophan ethyl ester were achieved below 0.1 μg mL −1 . The established method was successfully applied to the analysis of the target analytes in black sesame seed, which provided a simple, low-cost, and sensitive approach for the determination of trace compounds in complex samples.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2011
·Vol. 123
·期 3
·1675-1683
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IF 1.24
Nianjun Kang,Zhongjie Du,Hangquan Li,Chen Zhang,Zhehan Zhao
A series of β-cyclodextrin-based epoxy resins were synthesized with different epoxy equivalent weights. Their chemical structures were characterized with Fourier Transform Infrared Spectroscopy (FTIR) and Nuclear Magnetic Resonance Spectroscopy (NMR) examination. These epoxy resins were cured using L -arginine as a curing agent, and the degradation behavior of the cured resins was evaluated under different acidic buffer solutions at 37°C. The degradable behavior of such epoxy resins suggested potential applications as environment friendly materials. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
研究领域:
材料科学
·
化学工程
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CHEMICAL ENGINEERING RESEARCH & DESIGN
·
2017
·
IF 2.538
Shuangzhen Guo,Pengpeng Jiao,Zhigang Dan,Ning Duan,Jian Zhang,Guanyi Chen,Wubin Gao
A novel l -arginine modified magnetic bioadsorbent (Fe 3 O 4 -CS-L) was fabricated to remove Zn(II), Cd(II) and Pb(II) from aqueous solution by adsorption method. Several techniques such as FT-IR, SEM, XRD, TGA and VSM were used for characterizing the Fe 3 O 4 -CS-L. The results showed that the Fe 3 O 4 -CS-L was successfully prepared. Batch experiments were conducted to investigate the parameters affecting the adsorption of the novel magnetic bioadsorbent to metal irons, such as adsorption isotherm and adsorption kinetics. The adsorption equilibrium was commendably described by pseudo-second-order model. Langmuir model was better than Freundlich model to describe adsorption isotherm data and the predicted maximum adsorption capacities of Zn(II), Cd(II) and Pb(II) were 256.41 mg/g, 156.99 mg/g and 128.63 mg/g, respectively. Competition adsorption results showed that the adsorption order of Fe 3 O 4 -CS-L to metal ions was Pb(II) > Zn(II) > Cd(II). Moreover, regeneration studies suggested that Fe 3 O 4 -CS-L has a good recyclability. All the results showed that Fe 3 O 4 -CS-L could be a promising material in environment pollution cleanup.
研究领域:
环境科学
·
材料科学
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高影响因子
Journal of Macromolecular Science Part B-Physics
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2020
·
IF 0.911
Dan Wang,Jianjun Fang,Wenjuan Liu,Tianlong Huang,Yanmin Zhao,Jianglong Duan,Hailin Xie,Ping Chen,Ziwei Zhou,Zhihua Zhou
Poly(L-lactide-co-glycolide-co-ε-caprolactone) (PLLGC) and 1,4-butanediamine modified maleated poly(D,L-lactide-co-glycolide) (BMPLGA) were both synthesized by ring-opening polymerization with stannous octoate as catalyst. Then PLLGC/BMPLGA blend porous films were prepared by using NaCl particles as a porogen through a solvent casting/particle leaching method. The PLLGC/BMPLGA blend porous films were characterized by Fourier transform infrared spectroscopy, gel permeation chromatography and scanning electron microscopy. The tensile properties of the PLLGC/BMPLGA porous films were also studied. The results showed that the content and size of the NaCl porogens had significant effects on the porosity of the PLLGC/BMPLGA porous films. The tensile strength and elongation percentage of the porous films decreased with increasing porogen content. Thus, both the pore structure and mechanical properties of the porous PLLGC/BMPLGA blend films can be controlled by changing the amount and size of the porogen.
研究领域:
高分子科学
·
生物材料
·
组织工程
·
材料表征
·
聚合物共混
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高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Xuejuan Zhang,Yue Zhou,Guanlin Wang,Ziyu Zhao,Zhongxiang Jiang,Yingtong Cui,Xiao Yue,Zhengwei Huang,Ying Huang,Xin Pan,Chuanbin Wu
Poly-L-lysine (PLL) is a promising candidate for the treatment of pulmonary infection with lower occurrence of drug-resistance due to its unique antibacterial mechanisms. Dry powder inhalations (DPIs) are considered as the first choice for formulating PLL to treat pulmonary infection on account of direct delivery and satisfactory stability. However, hygroscopicity of PLL limited its therapeutic effect on pulmonary infection when PLL developed into DPIs. The hygroscopicity caused two obstacles including the low drug deposition in the lower respiratory tract and undesirable aerosolization performance deterioration. In this study, PLL was co-spray-dried with L-leucine (LL) to achieve moisture-resistance and desirable aerosolization performance. The ratio of PLL and LL was optimized to obtain particles with different morphology, hygroscopicity and aerodynamic properties. The obtained PLL DPIs were suitable for inhalation with a corrugated surface formed by hydrophobic LL. The anti-hygroscopicity, aerosolization performance and rheological properties of P 2 DPIs were optimal when PLL:LL = 85:15. The DPIs particles were stable after being stored at high relative humidity (60 ± 5%), and their superiority in treating pulmonary infections was also proved by in vitro and in vivo experiments. The established PLL DPIs were proved to be a feasible and desirable approach to treat pulmonary infections.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Xiuyun Guo,Feng Gao,Yawei Zhang,Zengqi Peng,Muneer Ahmed Jamali
The effects of l -histidine (His) and l -lysine (Lys) on the emulsifying and interfacial properties of porcine myofibrillar proteins (MPs) at low/high ionic strength were investigated to improve physical stability of MPs emulsion at low ionic strength. Results showed that His and Lys increased emulsion stability index, and decreased creaming index at low ionic strength, but caused no significant changes in both indexes at high ionic strength. Additionaly, His and Lys increase d the emulsifying activity and apparent viscosity, but decreased the droplet size at both ionic strength. His and Lys also promoted the diffusion of MPs and increase d the interfacial pressure. Meanwhile, the interfacial loading of MPs was increased (p < 0.05) by 16.07% and 12.79% at low ionic strength, and by 8.85% and 8.24% at high ionic strength in the presence of Lys and His, respectively. Raman spectra revealed that the α-helix content of MPs was decreased (p < 0.05) by 5.05% and 11.79% at low ionic strength, and by 12.14% and 7.23% at high ionic strength in the presence of Lys and His, respectively. In summary, His and Lys enhanced the stability of MPs emulsion at low ionic strength via changing the structure and interfacial behaviour of MPs.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2016
·
IF 3.13
Shuyan Zhao,Ziyan Fan,Lihui Sun,Tao Zhou,Yuliang Xing,Lifen Liu
A vegetation study was conducted to investigate the interactive effects of perfluoroalkyl substances (PFASs), including perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), and Cadmium (Cd) on soil enzyme activities , phytotoxicity and bioaccumulation of wheat ( Triticum aestivum L.) and rapeseed ( Brassica campestris L.) from co-contaminated soil. Soil urease activities were inhibited significantly but catalase activities were promoted significantly by interaction of PFASs and Cd which had few effects on sucrase activities. Joint stress with PFASs and Cd decreased the biomass of plants and chlorophyll (Chl) content in both wheat and rapeseed, and malondialdehyde (MDA) content, superoxide dismutase (SOD) and peroxidase (POD) activities were increased in wheat but inhibited in rapeseed compared with single treatments. The bioconcentration abilities of PFASs in wheat and rapeseed were decreased, and the translocation factor of PFASs was decreased in wheat but increased in rapeseed with Cd addition. The bioaccumulation and translocation abilities of Cd were increased significantly in both wheat and rapeseed with PFASs addition. These findings suggested important evidence that the co-existence of PFASs and Cd reduced the bioavailability of PFASs while enhanced the bioavailability of Cd in soil, which increased the associated environmental risk for Cd but decreased for PFASs.
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Journal of Materials Chemistry B
·
2016
·Vol. 5
·期 5
·935-943
·
IF 4.872
Guanglong Ma,Weifeng Lin,Zhefan Yuan,Jiang Wu,Haofeng Qian,Liangbo Xu,Shengfu Chen
Environmentally responsive hydrogels show enormous potential in various applications, such as tissue engineering and drug delivery. The site-specific controlled drug delivery of hydrogels can improve the therapeutic outcome and minimize the negative side effects. In this work, enzymatically digestible hydrogels, which are composed of equally mixed L-glutamic acid (E) and L-lysine (K) polypeptides after being crosslinked by the coupling reaction between carboxyl groups and primary amines catalyzed by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide·HCl (EDC·HCl), were prepared to improve the biocompatibility through reducing the nonspecific protein adsorption and cell attachment. Hydrogels loaded with two model drugs, doxorubicin hydrochloride (DOX·HCl) (positively charged anti-cancer drug) and diclofenac sodium (negatively charged anti-inflammatory drug), showed accelerated complete drug release and full enzymatic degradation in the presence of trypsin, which was reported to be expressed in various carcinomas and inflammations. The drug release also responds to the pH change through tuning charge–charge interaction. These indicated that the prepared hydrogels were promising candidates for drug delivery systems.
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Journal of Pharmaceutical Analysis
·
2019
·
IF 4.44
Dongsheng Xu,Elena Sánchez-López,Qiqin Wang,Zhengjin Jiang,María Luisa Marina
An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl (FMOC) derivatized amino acids by nano-liquid chromatography. The mobile phase was optimized including the apparent pH, content of ACN, and concentration of the buffer to obtain a satisfactory enantioresolution performance. 27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested, and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of them. Analytical characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity, precision, accuracy, limits of detection (LOD) and quantitation (LOQ) showing good performance to be applied to the enantiomeric determination of these amino acids in dietary supplements. LOD and LOQ values were 9.3 and 31 μM for norvaline enantiomers and 7.5 and 25 μM for tryptophan enantiomers, respectively. The contents of d -norvaline and d -tryptophan were below their respective LODs in all the analyzed samples. Quantitation of l -tryptophan and l -norvaline showed good agreement with the labeled contents except for one sample which did not show presence of l -norvaline, contrary to the label indication.
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JOURNAL OF PHYSICAL CHEMISTRY B
·
2021
·
IF 2.991
Bin-Cheng Wang,Xiu-Ping Li,Biao-Biao Hao,Chen-Xi Zhang,Qing-Lun Wang
A coordination polymer with dual functions of high proton conductivity and highly sensitive fluorescent sensors demonstrates a great application potential. In this work, a cadmium-based coordination polymer (denoted as CP 1) with hydrothermal stability was synthesized. The abundant coordination water, lattice water, and amino groups make an extended hydrogen-bonding pathway for efficient proton migration, which endows CP 1 with the highest proton conductivity of 2.41 × 10–3 S·cm–1 at 353 K and 98% RH. Especially, the proton conductivity of the chitosan (CS) hybrid membrane containing CP 1 reaches a maximum value of 2.62 × 10–2 S·cm–1 under 343 K and 98% RH, which increases almost 7 times higher than that of the pure CS membrane due to the host–guest collaboration. Furthermore, luminescence studies revealed that CP 1 is a high-sensitivity and good-selectivity fluorescent probe for the detection of trace amounts of l-histidine with a lowest detection limit of 1.0 × 10–8 M.
研究领域:
材料科学
·
化学
·
生物传感
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高影响因子
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