NEUROPHARMACOLOGY
·
2025
·
IF 4.6
Yufang Lu,Ruiying Zhou,Ruyi Zhu,Xue Wu,Jin Liu,Yue Ma,Xin Zhang,Yaling Zhang,Luting Yang,Yanhua Li,Yuan Zhang,Yaping Yan,Qian Zhang
This study aims to elucidate the target and mechanism of baicalin, a clinically utilized drug, in the treatment of neuroinflammatory diseases. Neuroinflammation, characterized by the activation of glial cells and the release of various pro-inflammatory cytokines, plays a critical role in the pathogenesis of various diseases, including spinal cord injury (SCI). The remission of such diseases is significantly dependent on the improvement of inflammatory microenvironment. Toll-like receptor 4/myeloid differentiation protein 2 (TLR4/MD2) complex plays an important role in pathogen recognition and innate immune activation. baicalin, a natural flavonoid, is renowned for its potent anti-inflammatory property. In this study, we discovered that baicalin significantly reduced the activation of glial cells and the levels of pro-inflammatory cytokines at the lesion site of SCI mice, thereby mitigating demyelination and neuronal damage. By directly occupying the active pocket of TLR4/MD2 complex on microglia, baicalin inhibited PI3K/AKT/NF-κB pathway, thereby exerting its anti-inflammatory effect. These findings were corroborated in mice induced by lipopolysaccharide, a TLR4 agonist. Furthermore, baicalin indirectly altered phenotype of astrocytes by reducing secretion of TNF-α, IL-1α, and C1q levels from microglia. Our work demonstrated that baicalin effectively alleviated neuroinflammation by directly targeting microglia and indirectly modulating astrocytes phenotype. As a natural flavonoid, baicalin holds significant potential as a therapeutic candidate for diseases characterized by neuroinflammation.
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PHYTOMEDICINE
·
2025
·
IF 8.3
Jiao Liu,Danye Niu,Yu Tang,Ruoheng Zheng,Yinyin Qin,Xiuqin Cheng,Shubo Pan,Jinfei Yuan,Xiaohua Shi,Jiao Yang
Background β-Hydroxy β-Methylbutyrate (HMB), derived from leucine, is known for its role in anti-oxidation and anti-inflammation. But, the application of HMB in IBD treatment is not fully understood, highlighting the requirement for further research. Purpose We aimed to examine the effects of HMB treatment on DSS-induced chronic colitis in mice and explore its underlying mechanisms. Methods To simulate colonic inflammation, a murine colitis model was generated by using DSS induction. Critical indicators such as body weight, colon length, disease activity index (DAI), and gross pathology were thoroughly monitored. Immunohistochemistry assay was conducted to assess the expression of Occludin and F4/80. Flow cytometry was employed to evaluate the expression levels of CD80 and CD86. qPCR was performed to measure cytokine expression (IL-6, IL-1β, TNF-α, IL-22, CXCL2, iNOS). RNA sequencing was carried out using bone-marrow derived dendritic macrophage cells (BMDMs). Results Our study indicates that HMB treatment substantially mitigated colonic damage in murine models of DSS-induced colitis, highlighting its anti-inflammatory potential. Notably, HMB significantly enhanced the expression of Occludin in these mice. Furthermore, HMB downregulated proinflammatory markers such as IL-6, IL-1β, and TNF-α as well as CXCL2 in the colon tissue. In vitro experiments also revealed that HMB reduced production of proinflammatory cytokines induced by DSS and suppressed the expression levels of CD80 and CD86 in macrophage cells. On a mechanistic level, we demonstrated the anti-inflammatory effects of HMB by reducing the phosphorylation of p-ERK and p-p65, thereby limiting cytokine production in both in vivo and in vitro settings. Conclusion These findings indicate that HMB possesses anti-inflammation against intestinal inflammation and may hold promise as a potential therapeutic candidate for IBD treatment. There's growing interest in combining traditional anti-inflammatory agents with supplements like HMB to improve outcomes in complex IBD cases. HMB's role in established muscle preservation and reduction of systemic inflammation as described in this study could make it a valuable adjunct in IBD therapy.
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JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2024
·Vol. 99
·期 12
·2693-2702
·
IF 2.8
Yandong Lu,Jiaxu Ning,Yue Zong,Fu Ding,Gaige Liu,Ruijun Zhang
BACKGROUND Tailored design of high-performance nanofiltration membranes is desirable. The physicochemical properties of the organic phaseare essential for the preparation of nanofiltration membranes. Therefore, byfine-tuning the organic phase, it is possible to achieve nanofiltrationmembranes with enhanced performance. RESULTS In this work, oleic acid, a naturally extracted fatty acid, wasintroduced into three different organic solvents (n-heptane, n-octane andisopar G) to construct special oil phases for interfacial polymerization. Testingresults showed that proper dosage of oleic acid addition can effectivelydecrease the nanofiltration membrane pore size and enhance the salt rejectionregardless of the solvent type. Taking n-octane as example, 5% oleic acidaddition in it can decrease the mean pore size from 0.500 nm to 0.341 nm and improve the MgSO 4 rejection from 52.4% to 95.5%. Further characterizationindicated that the introduction of oleic acid into organic solvent cansignificantly reduce the interfacial tension and promote the diffusion of piperazinefrom aqueous phase to the oil phase. In addition, oleic acid could react with piperazineand produce some white particles that could be embed into the polyamide layer, thus altering the surface morphology. CONCLUSION In this work, a novel modified thin film composite nanofiltration membrane was prepared by a simple and controllable oleic acid assisted interfacial polymerization strategy, which exhibits good water permeability, solute selectivity and antifouling property. Without changing the existing process, our strategy opens up a simple, environmentally friendly and operable route for the synthesis of thin film composite nanofiltration membranes. © 2024 Society of Chemical Industry (SCI).
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2025
·Vol. 29
·期 15
·e70778
·
IF 4.2
Hong Tang,Qi Dai,Ziyan Zhao,Weiye Ge,Danlei Li,Zelin Chang,Penglai Pi,Jia Li,Zheng Sun
Glioblastoma (GBM) is an aggressive and highly malignant primary brain tumour, accounting for a significant proportion of adult brain tumours. It is associated with a poor prognosis and high recurrence rates. Although temozolomide (TMZ) remains the standard first-line chemotherapy for GBM, its clinical efficacy is often limited by the development of drug resistance and toxic effects on normal tissues. Melatonin (Mel), a natural indoleamine synthesised by the pineal gland, has demonstrated synergistic anti-tumour effects when combined with various chemotherapy agents in multiple studies. This study investigates the synergistic potential of Mel to enhance TMZ's therapeutic efficacy against GBM. The results demonstrate that the combination of Mel and TMZ significantly inhibits glioblastoma cell proliferation, migration, and invasion. Mechanistically, this synergistic effect is mediated through the NF-κB/COX-2 signalling pathway. Mel enhances TMZ's anti-tumour activity by inhibiting IκBα phosphorylation, suppressing NF-κB activation, and downregulating COX-2 expression. Additionally, the combination treatment induced apoptosis via activation of the Caspase-3 pathway. These results suggest that Mel can potentiate the therapeutic efficacy of TMZ in glioblastoma treatment, offering a promising strategy to overcome TMZ resistance while reducing its associated toxicity.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 3.7
Jia Cheng,Yang Xiang,Xun Huang,Zidong Wei
Electrocatalytic oxidation of surplus ethylene glycol (EG) to high-value glycolic acid (GA) represents a promising approach for sustainable resource utilization, though critical challenges persist in developing durable electrocatalysts and achieving effective recovery of the free acid product from its salt derivatives in alkaline electrolytes. In this work, a PdNi/NF catalyst was rationally synthesized via a one-step electrodeposition method. Systematic characterization revealed that the electron transfer from Ni to Pd modulates *OH adsorption to accelerate EG oxidation reaction (EGOR) while preventing Pd deactivation through oxidation. The optimized system demonstrated exceptional alkaline performance with a glycolic acid Faraday efficiency of 95% and a current density of 666 mA cm -2 . When implemented in an asymmetric EGOR||HER flow cell configuration where only the cathodic electrolyte contains alkaline, the system demonstrated exceptional operational stability by sustaining 70 mA cm -2 current density at a low cell voltage of 0.9 V in neutral media for over 100 h, with product glycolic acid requiring no further acidification. This investigation provides a practical framework for designing efficient electrocatalytic systems that simplifies product separation steps.
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International Journal of Medical Sciences
·
2025
·Vol. 22
·期 15
·3839-3853
·
IF 3.2
Wen Weijia,Li Jiaying,Yuan Li,Liao Yan,Shao Caixia,Xu Dingze,Jiang Hongye,Liao Yuandong,Liu Pan,Zhang Chunyu,Yao Shuzhong,Wang Wei
Lymph node metastasis (LNM) confers significant treatment failure and adverse clinical outcomes in cervical cancer (CCa). However, large unknown lies in the mechanisms underlying LNM in CCa. In this study, we discovered that PRDX3 is elevated in CCa with LNM and associates with poor prognosis in CCa patients. Moreover, multivariate logistic analysis revealed that PRDX3 is an independent predictor of LNM in CCa. Functional investigations demonstrated that PRDX3 promotes invasion, lymphangiogenesis, and LNM of CCa. Mechanistically, PRDX3 activates NF-κB signaling pathway and upregulates the downstream pro-metastatic molecules VEGF-C and MMP-9 in CCa cells. Furthermore, PRDX3 could inhibit the anoikis of detached CCa cells by reducing the ROS level and hence promote LNM in CCa. Importantly, genetic inhibition of PRDX3 potently slows LNM of CCa. These findings highlight a novel PRDX3-mediated mechanism of LNM in CCa and recognize PRDX3 as a promising predictive marker and target of clinical intervention for LNM in CCa.
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Materials
·
2024
·Vol. 17
·期 10
·2195
·
IF 3.1
Yu Liu,Panpan Li,Zegao Wang,Liangjuan Gao
This study reported a multi–functional Co0.45Fe0.45Ni0.9–MOF/NF catalyst for oxygen evolution reaction (OER), hydrogen evolution reaction (HER), and overall water splitting, which was synthesized via a novel shape–preserving two–step hydrothermal method. The resulting bowknot flake structure on NF enhanced the exposure of active sites, fostering a superior electrocatalytic surface, and the synergistic effect between Co, Fe, and Ni enhanced the catalytic activity of the active site. In an alkaline environment, the catalyst exhibited impressive overpotentials of 244 mV and 287 mV at current densities of 50 mA cm−2and 100 mA cm−2, respectively. Transitioning to a neutral environment, an overpotential of 505 mV at a current density of 10 mA cm−2was achieved with the same catalyst, showing a superior property compared to similar catalysts. Furthermore, it was demonstrated that Co0.45Fe0.45Ni0.9–MOF/NF shows versatility as a bifunctional catalyst, excelling in both OER and HER, as well as overall water splitting. The innovative shape–preserving synthesis method presented in this study offers a facile method to develop an efficient electrocatalyst for OER under both alkaline and neutral conditions, which makes it a promising catalyst for hydrogen production by water splitting.
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FASEB JOURNAL
·
2025
·Vol. 39
·期 3
·e70379
·
IF 4.2
Jiancheng Yang,Qinghua Tang,Siyu Li,Lei Yang,Ming Yang,Qingmei Li,Yan Feng,Mingming Pan,Yuhong Zeng
The accumulation of intracellular reactive oxygen species (ROS) is widely recognized to stimulate the development of osteoclasts, a crucial factor in the onset of osteoporosis. The ROS scavenging capability of Prussian blue nanoparticles (PBNPs) is exceptional, and they possess excellent biocompatibility. However, the effects of PBNPs on osteoporosis remain unknown. Present study aimed to investigate whether PBNPs could inhibit osteoclast differentiation and prevent ovariectomy (OVX)-induced bone loss by suppressing ROS. In vitro experiments demonstrated that PBNPs attenuated osteoclastogenesis and downregulated the expression of osteoclast-related genes. Mechanistically, PBNPs reduce cellular ROS by blocking RANKL-induced ROS generation and increasing the expression of ROS scavenging enzymes, which in turn block the NF-κB, ERK, JNK, and p38 pathways, thereby reducing NFATc1 signaling. According to in vivo experimental results, OVX caused a significant rise in ROS in the bone marrow, an increase in osteoclastic number on the bone surface, and substantial bone loss; however, PBNPs significantly reduced ROS and successfully protected OVX-induced bone damages. In conclusion, this study showed for the first time that PBNPs prevented OVX-induced bone loss and attenuate osteoclastogenesis, indicating that PBNPs may be a viable alternative therapy for the management of osteoclast-associated bone disorders. Graphical Once RANKL binds to RANK on the cell membrane of BMMs, the NF-κB and MAPK pathways become activated, resulting in the amplification of NFATc1, which upregulates the expression of osteoclast-specific genes such as CTSK , Integrin αV , Atp6v0d2 , and MMP9 . These signaling events are mediated by the RANKL-induced ROS signaling. PBNPs decreased levels of ROS and mitigated these signaling pathways by decreasing ROS production and enhancing the expression of antioxidant enzymes. Consequently, PBNPs prevented osteoclastogenesis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shanbo Ma,Rui Zhang,Long Li,Jin Wang,Meiling Zheng,Xiaodi Guo,Shan Miao,Wei Quan,Wenjuan Liu,Xiaopeng Shi
The current study isolated a homogeneous polysaccharide (AP) with a molecular weight of 7.9 kDa from the pomace of Fuji apples. AP was found to consists of rhamnose, galactose, arabinose, glucose, and galacturonic acid in a ratio of 4.3:5.2:2.6:1.0:11.9. Ten sugar residues in AP, including T-Ara f , 1,5-Ara f , 1,2-Rha p , 1,3-Rha p , T-Gal p , 1,3,5-Ara f, 1,4-Gal p , 1,4-Gal p A, 1,6-Glc p , and 1,3,6-Glc p were identified using methylation and GC–MS. Combined with 1D and 2D NMR, it was further revealed that AP possesses a backbone of α-Gal p- (1 → [3)-α-Rha p -(1 → 2)-α-Rha p -(1] 2 → [4)-α-Gal p A-(1] 10 → 3,6)-β-Glc p -(1 → 6)-β-Glc p -(1 → 4)-β-Gal p -(1 → 4)-β-Gal p -(1→, with two branches: α-Ara f -(1 → 5)-α-Ara f -(1 → 5)-α-Ara f -(1 → 3,5)-α-Ara f -(1 → 6)-β-Glc p -(1→ and →3)-α-Rha p -(1 → 5)-α-Ara f -(1 → 3,6)-β-Glc p -(1→ bonded to the C-3 of β-1,3,6-Glc p . AP significantly inhibited the release of cytokines and inflammatory mediators, such as TNF-α, IL-1β, IL-6, reactive oxygen species (ROS) and nitric oxide (NO). Western blotting results indicated that AP treatment markedly downregulated iNOS and NF-κB protein expression in LPS-induced RAW264.7 cells, leading to decreased levels of phosphorylated proteins (p-NF-κB and p-ΙκΒα) and preventing the degradation of ΙκΒα. Furthermore, in LPS-induced RAW264.7 macrophages, AP inhibited the expression of TLR4 protein, which in turn inhibited the activity of the NF-κB pathway. The findings demonstrated that AP exhibits anti-inflammatory properties in vitro by targeting the TLR4/NF-κB signaling pathway, thus impeding the nuclear translocation of NF-κBp65, suppressing the expression of related pro-inflammatory factors.
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Hepatology International
·
2025
·
IF 6.1
Yang Yanfang,Wang Tao,Fu Yuyin,Li Xukui,Yu Fuxun
Background Hepatitis B virus (HBV) is a pathogen that poses a serious threat to human health. The interaction between HBV and host has made great progress in recent years. SUMOylation is involved in virus-related cancer progression, but there are fewer studies on the mechanism of SUMOylation on HBV replication and antiviral defense. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a critical adaptor of the NF-κB pathways. Here, we focus on the roles of TRIM28 in regulating TRAF6 SUMOylation in HBV-replicating cells. Methods The SUMO1-modified TRAF6 proteins were enriched from total cellular proteins by immunoprecipitation with anti-SUMO1 antibody, then the SUMOylated TRAF6 was detected by western blot using an anti-TRAF6 antibody. The interaction between TRAF6 and TRIM28 was identified by immunoprecipitation and LC–MS/MS. The modification sites of TRAF6 SUMOylation were identified by amino acid site mutation. Expression and localization of TRAF6 and TRIM28 were assessed by immunohistochemistry and immunofluorescence. The hydrodynamic injection HBV mouse model was used to determine the function of TRIM28-mediated TRAF6 SUMOylation in vivo. Results The results show that the levels of SUMO1-modified TRAF6 are elevated in HBV-replicating cells. Lys453 is a major SUMO1 modification site of TRAF6. There is an antagonistic interaction between SUMOylation and ubiquitination of TRAF6 protein. The SUMO ligase TRIM28 is responsible for catalyzing TRAF6 SUMOylation. Compared to the wild-type TRAF6, its SUMO site mutant TRAF6 K453R promotes NF-κB activation. Moreover, TRIM28 overexpression attenuates TRAF6-mediated NF-κB activation, thereby inhibiting HBV replication in vivo. Conclusions Our findings demonstrate that SUMO ligase TRIM28 affects the ability of TRAF6 on NF-κB activation, nucleocytoplasmic shuttling and HBV replication-related indicators. Our data reveal that TRIM28-mediated SUMOylation of TRAF6 is a novel mechanism to regulate the inflammatory response, which may pave the way for new strategies to control anti-HBV.
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International Journal of Electrochemical Science
·
2024
·
IF 1.3
Dandan Liang,Weili Hou,Ying Zhang,Jihua Shang,Haibo Ren,Yufeng Sun
Due to the prevailing energy shortages, the pursuit of new alternative energy sources is becoming increasingly urgent. Hydrogen production through water electrolysis has emerged as a crucial method. However, the sluggish four-electron reaction in the oxygen evolution reaction (OER) remains the primary rate-limiting step. In this study, we synthesized a heterostructure La(OH) 3 @CuO-P material on the nickel foam (NF) substrate. The experimental results demonstrate that after phosphating treatment, the heterostructure La(OH) 3 @CuO-P exhibits exceptional catalytic performance with only 215 mV overpotential, a Tafel slope value of 79.36 mV/dec, and a bilayer capacitance of 39.02 mF/cm 2 at a current density of 10 mA/cm 2 for OER in a 1 M KOH solution. These values are significantly superior compared to those obtained using heterostructure La(OH) 3 @CuO alone which showed an overpotential value of 365 mV at a current density of 10 mA/cm 2 . Moreover, during cyclic voltammetry testing for up to 500 cycles, La(OH) 3 @CuO-P also demonstrates relatively stable performance. Analyses suggest that composite heterostructure effectively addresses issues such as insufficient conductivity of La(OH) 3 and monomer aggregation tendency for CuO while maintaining excellent properties for each component. By incorporating P atoms as dopants, the interaction between La, Cu, and P atoms not only facilitates fine-tuning of the electronic structure and optimization of the adsorption free energy (-OH), but also promotes an increase in catalytic active sites through sample size reduction, thereby further augmenting the performance of the OER.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Yushi Han,Qin Yin,Shuo Geng
The development of the liquor industry urgently needs to solve the problem of wastewater treatment. The electrochemical oxidation method, with its advantages of high efficiency and environmental friendliness, has surpassed traditional processes. The low electro-oxidation potential of organic alcohols in wastewater (significantly lower than the oxygen evolution reaction, OER) endows it with dual value: it can replace the anode OER while simultaneously achieving pollutant degradation and driving the hydrogen evolution reaction (HER) at the cathode, achieving the synergy of “anode pollution control and cathode hydrogen production”. Based on this, this study constructed a Fe-NiOOH/NiMo/NF heterojunction catalyst, which, through the synergy of the efficient alcohol degradation ability of Fe-NiOOH and the excellent hydrogen evolution performance of NiMo, established a stable coupling system for wastewater degradation and hydrogen evolution at high current densities. Experiments confirmed that: Experimentally, the Fe-NiOOH/NiMo/NF anode delivers 200 mA cm −2 at 1.434 V vs. RHE; the NiMo/NF cathode reaches 20 mA cm −2 at an overpotential of 88 mV; and the full cell (Fe-NiOOH/NiMo/NF=|NiMo/NF) sustains 100 mA cm −2 for 100 h with only a 30 mV rise in voltage. Compared with the high-purity alcohol-based OER replacement scheme, using low-cost liquor wastewater as the anode reactant significantly reduces the cost of hydrogen production. The stable operation of this synergy system at high current densities provides a reliable technical path for the industrialized wastewater degradation and hydrogen production.
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