JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yalin Xie,Rui Tan,Min Hu,Xiaojun Luo,Xiaojun Deng,Yuanyuan Li
Certain diuretics have been identified as potential contaminants in pharmaceutical and food products, posing a risk to food safety and public health. In this study, a surface-enhanced Raman spectroscopy (SERS) detection method based on Silver nanostars (Ag NSs) was developed for the detection of motion-prohibited diuretics in food and biological samples. Ag NSs synthesized via a simple solution-based method exhibited a star-like morphology with high-density "hot spot", achieving an enhancement factor of 1.0 × 10 5 —3 orders of magnitude higher than that of traditional spherical silver nanoparticles. A highly sensitive SERS detection system was established for bumetanide (limit of detection: 7.08 × 10 −9 g/mL), triamterene (1.13 × 10⁻⁸ g/mL), and dorzolamide (1.04 × 10⁻⁸ g/mL) for the first time. Characteristic fingerprint peaks enabled accurate molecular identification. The method was validated in milk and urine with recoveries of 94.2 %–104.9 %, and relative standard deviation (RSD) < 7 %. Results were consistent with those obtained by HPLC-HRMS/MS, confirming the method's reliability. The proposed system offers advantages of rapid detection (<10 min), low cost, and minimal matrix interference, enabling efficient multi-residue analysis in complex samples. This work provides a promising tool for food safety monitoring and anti-doping surveillance, contributing to effective regulatory enforcement.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2010
·
IF 2.6
Chunyuan Song,Zhuyuan Wang,Jing Yang,Ruohu Zhang,Yiping Cui
A detailed method for preparation of 2-mercaptobenzothiazole (MBT)-labeled immuno-Au aggregates as a novel Raman probe for SERS-based immunoassay has been proposed in this paper. The formation kinetics of gold aggregates induced by MBT and the impact of the amount of labeled reporter on the aggregation, as well as the fabrication of MBT-labeled immuno-Au aggregates were characterized comprehensively by UV–vis spectrophotometer . Meanwhile, a chain-like morphology of aggregates was monitored by TEM images. Experimental results show that this Raman tag can act as an efficient label and an enhanced Raman signal of SERS-based immunoassay can be obtained successfully with this novel probe. This work shows the method for the preparation of immune probe for SERS-based immunoassay using MBT as reporter and provides a reference for the preparation of immune probe with other Raman markers for multi-channel SERS-based immunoassay.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yanbo Wang,Haojie Ni,Huan Li,Jian Chen,Dequan Zhang,Linglin Fu
Microbial contamination of food is a critical public health issue worldwide, making the rapid, efficient, and safe sensing strategies for foodborne microbes in urgent need. In this work, a plasmonic microneedle (MN) array patch was developed for rapid extraction and surface-enhanced Raman spectroscopy (SERS) detection of a model bacteria, Escherichia coli ( E. coli ). A hydrophilic MN matrix was firstly prepared by crosslinking [2-(methacryloyloxy)ethyl]trimethylammonium chloride (TMA), 2-carboxyethyl acrylate (CAA), and 2-hydroxyethyl methacrylate (HEMA) in a micro-mold. Then the obtained MN patch was treated with ozone and coated with SERS-active gold nanopopcorns (GNpops) that were modified with the specific aptamer towards E. coli . The resultant plasmonic MN patch could extract E. coli from agarose simulant in 3 min and was capable of on-MN SERS analysis with a detection limit of 143 CFU/g. The proposed detection method exhibited great specificity to E. coli and was feasible for evaluating the E. coli contamination of commercial mutton samples. Moreover, the extracted bacteria could be effectively inactivated through photothermal sterilization. The work opened a new avenue for simplifying the detection procedures for microorganisms and provided a potential approach for rapid and on-site microbial monitoring through the SERS-active MN patch.
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高影响因子
Food Analytical Methods
·
2018
·Vol. 12
·期 2
·595-603
·
IF 2.245
Shen Mofei,Duan Nuo,Wu Shijia,Zou Ying,Wang Zhouping
This paper investigated a new detection method for food pathogenic bacteria Vibrio parahaemolyticus based on surface-enhanced Raman scattering (SERS). Nanostructures consisting of Aptamer-Au nanoparticles-polydimethylsiloxane (Apt-Au-PDMS film) were firstly used as SERS substrate. Aptamers were immobilized on the functionalized PDMS film covered with Au nanoparticles (AuNPs) to act as a capture structure via the affinity binding of aptamer, and Vibrio parahaemolyticus . AuNPs were modified with the aptamer and Raman reporter molecule 4-mercaptobenzoic acid (4-MBA) to act as SERS-sensing probes that bind to the target in the same way as the Apt-Au-PDMS film. The solid sandwich structure was formed by capture structure-target-SERS-sensing probes. The concentration of Vibrio parahaemolyticus can be quantified by measurement of the SERS intensity of 4-MBA. Under optimal conditions, the signal of 4-MBA at 1590 cm −1 was linearly related to the Vibrio parahaemolyticus concentration in the range between 1.2 × 10 2 and 1.2 × 10 6 cfu·mL −1 . Recoveries ranging from 97.7% to 105.3% were found when analyzing spiked prawn samples. This developed ultrasensitive aptamer-based SERS detection structure suggested that it would be a promising strategy for a variety of sensing applications.
研究领域:
分析化学
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食品安全
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纳米技术
·
微生物学
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2016
·
IF 3.169
Yan Lin,Guanhong Xu,Fangdi Wei,Aixia Zhang,Jing Yang,Qin Hu
In this present work, a rapid and simple method to detect carcinoembryonic antigen (CEA) was developed by using surface-enhanced Raman spectroscopy (SERS) coupled with antibody-modified Au and γ-Fe 2 O 3 @Au nanoparticles. First, [email protected] reporter and γ-Fe 2 O 3 @Au were prepared, and then modified with CEA antibody. When CEA was present, the [email protected] reporter and immuno-γ-Fe 2 O 3 @Au formed a complex through antibody-antigen-antibody interaction. The selective and sensitive detection of CEA could be achieved by SERS after magnetic separation. Under the optimal conditions, a linear relationship was observed between the Raman peak intensity and the concentration of CEA in the range of 1–50 ng mL −1 with an excellent correlation coefficient of 0.9942. The limit of detection based on two times ratio of signal to noise was 0.1 ng/mL. The recoveries of CEA standard solution spiked with human serum samples were in the range of 88.5–105.9% with the relative standard deviations less than 17.4%. The method built was applied to the detection of CEA in human serum, and the relative deviations of the analysis results between the present method and electrochemiluminescence immunoassay were all less than 16.6%. The proposed method is practical and has a potential for clinic test of CEA.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
癌症诊断
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PubMed
高影响因子
APPLIED OPTICS
·
2020
·Vol. 59
·期 35
·11070-11075
·
IF 1.961
Yaoyao Yin,Meng Wang,Tian Xu,Cheng Yin,Yonglong Jin
Different from ordinary planar waveguide structure, we designed a double-metal-cladding waveguide (DMCW) for easier light coupling into the guiding layer from free space. In contrast to evanescent waves in a surface plasmon polariton waveguide, an oscillating wave is generated in the guiding layer. and a similar Fabry–Perot (FP) cavity can be formed by the DMCW. In past work, the FP cavity excited by the DMCW was used to study the refractive index of light, while in this work, the FP cavity is used to excite the photothermal effect of the metal substrate. It is a good connection between light and heat. The photothermal effect is investigated to promote the galvanic replacement reaction in the substrate. Although the experiment process is destructive to the DMCW structure, a surface-enhanced Raman scattering (SERS) chip is prepared on the basis of the photothermal effect in the DMCW. It shows that the DMCW can convert the energy of incident light into thermal energy, and then prepare the SERS chip. The chip has better uniformity, stronger activity, and higher sensitivity. The results demonstrate that the morphology of the SERS substrate created via the DMCW is far more elaborate than that via the surface plasmon polariton waveguide.
研究领域:
光学
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材料科学
·
纳米技术
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Yuan Gan,Shen Shen,Ning Wang,Jie Zhang
Surface-enhanced Raman scattering (SERS) technology faces several challenges in real-world applications, including low sensitivity, irreversible contamination of the substrate leading to poor cyclic stability, and difficulties in achieving integration of complex detection processes. To address these challenges, we propose a microfluidic-SERS integrated detection chip with dynamic potential modulation, enabling a complete “detection–self-cleaning–re-detection” closed-loop operation. Firstly, a high-sensitivity core-shell Ag@SiO 2 substrate was prepared using chemical reduction, achieving a detection limit of 10 −12 mol/L for Rhodamine 6 G (R6G) (AEF = 5.7 × 10 9 ). Plasma surface cleaning was then applied to bond polydimethylsiloxane (PDMS) microfluidic channels with screen-printed electrodes (SPEs), resulting in the construction of a microfluidic-SERS chip with dynamic potential modulation. By applying a positive potential (+2.0 V/10 s), target molecules were desorbed from the metal nanoparticle gaps on the SERS substrate, and a metastable metal oxide layer of a certain thickness was formed. Subsequently, a negative potential (−1.2 V/15 s) was applied to remove part of the metal oxide layer, realizing in-situ regeneration of Raman hotspots and rapidly driving probe molecule enrichment. After eight cycles of detection, the signal strength remained high. Additionally, this platform can precisely identify multi-molecule mixtures (R6G/CV/MG) and has been successfully applied to the detection of uric acid molecules. Its on-site detection capability was further validated in a portable system, providing an innovative solution for rapid detection in complex real-world environments.
研究领域:
分析化学
·
生物传感器
·
纳米医学
·
即时检测
·
分子诊断
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产品关联
DOI
高影响因子
Surfaces
·
2025
·Vol. 8
·期 4
·84
·
IF 2.9
Quanping Diao,Liyang Sun,Linlin Lv,Tiechun Li,Jiaqi Pan,Weiwei Luo
Hexaconazole, a triazole-class fungicide, demonstrates broad-spectrum protective and therapeutic activity against fungal pathogens, particularly those from Basidiomycota and Ascomycota, such as brown spot and powdery mildew. Despite its efficacy in controlling Actinidia brown spot disease in kiwifruit, excessive hexaconazole residues pose significant health risks due to its high toxicity. To address this challenge, a rapid analytical method for detecting hexaconazole residues in kiwifruit was developed using surface-enhanced Raman spectroscopy (SERS). The methodology employed silver colloid (C-AgNPs) as the active substrate and 1 mol/L NaCl as the aggregation agent, optimized through systematic testing, resulting in an optimal volume ratio of 400:225 between C-AgNPs and hexaconazole solution and a sequential mixing order of C-AgNPs + NaCl + Hexaconazole, followed by a 20 min incubation period. The characteristic Raman peak at 1584 cm−1was identified as the spectral signature for hexaconazole quantification. Analytical validation revealed a linear detection range of 0.25–2.25 mg/L (R2= 0.9870), precision with a relative standard deviation (RSD) of 1.7%, and an average recovery rate of 88.40–105.50%, confirming the method’s robustness. This approach enables rapid, non-destructive analysis with minimal sample pretreatment, offering high sensitivity and stability. This method demonstrates great potential for detecting hexaconazole residues in agricultural products.
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产品关联
DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Ying Zheng,Zhuo Chen,Chengbin Zheng,Yong-Ill Lee,Xiandeng Hou,Li Wu,Yunfei Tian
A facile method was developed for determination of trace volatile acetone by coupling a derivatization reaction to surface-enhanced Raman scattering (SERS). With iodide modified Ag nanoparticles (Ag IMNPs) as the SERS substrate, acetone without obvious Raman signal could be converted to SERS-sensitive species via a chemical derivatization reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH). In addition, acetone can be effectively separated from liquid phase with a purge-sampling device and then any serious interference from sample matrices can be significantly reduced. The optimal conditions for the derivatization reaction and the SERS analysis were investigated in detail, and the selectivity and reproducibility of this method were also evaluated. Under the optimal conditions, the limit of detection (LOD) for acetone was 5 mg L −1 or 0.09 mM (3σ). The relative standard deviation (RSD) for 80 mg L −1 acetone (n=9) was 1.7%. This method was successfully used for the determination of acetone in artificial urine and human urine samples with spiked recoveries ranging from 92% to 110%. The present method is convenient, sensitive, selective, reliable and suitable for analysis of trace acetone, and it could have a promising clinical application in early diabetes diagnosis.
研究领域:
分析化学
·
医学诊断
·
纳米技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Ming-zhi Zhang,Zhi-ming Zhou,Jing Xu,Wei-li Wang,Shu-huan Pu,Wei-ye Hu,Ping Luo,Zhong-qun Tian,Zhen-bin Gong,Guo-kun Liu
Antibiotics are widely used in daily life, which has created a global scenario where many pathogenic organisms have become effectively resistant to antibiotics. The abuse or overuse of antibiotics causes significant environmental pollution and even endangers human health. It is well-known that antibiotics' efficacy (toxicity) is determined by molecular structure. Therefore, structure-level qualitative analysis with high sensitivity and accuracy is vitally important. Characterized by fingerprinting recognition, Raman spectroscopy, especially surface-enhanced Raman spectroscopy (SERS), has become an essential qualitative analysis tool in various fields, such as environmental monitoring and food safety. With the exception of chirality, this study completed the qualitative trace analysis of 16 quinolone antibiotics (QNs) with fine molecular structure differences using SERS. The sensitivity was tuned in by one order of magnitude through the different electronegativity and steric hindrances of the slightly changed functional groups in the specific antibiotics. The fine structure dependent sensitivity enables SERS to be a powerful on-site monitoring tool to control the abuse of antibiotics with high toxicity; thus, decreasing the subsequent risk to the environmental ecology and human society.
研究领域:
环境科学
·
分析化学
·
药理学
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DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Xiangyu Fan,Hao Zhang,XinRu Zhao,Ke Lv,Tiying Zhu,Yaping Xia,Cheng Yang,Chengjie Bai
Three-dimensional (3D) SERS substrate with the denser “hotspots” is synthesized by the constriction of PDMS film decorated with sandwiched [email protected] @graphene ( [email protected] @G) nanostructure . Graphene layers above and below the AgNPs are used to absorb molecules onto the “hotspots”, and prevent the oxidation of AgNPs in our design. PDMS films can be easily shrunk for 3D structures, causing advantages in enhancement ability and light-matter interaction. Benefiting from the above advantages, a detection limit of 10 −14 M (CV) and enhancement factor (EF) of 3.9 × 10 9 were obtained in our experiment. Theoretical analyses (FDTD) were also used to study the enhancement mechanism. For practical purposes, in-situ detection of MG molecules on the fish surface and the label-free detection of DNA base of adenine (A) and cytosine (C) were also studied. The high enhancement factor, great sensitivity, reliability, and stability of substrate reasonably proved that it can be used as an excellent SERS substrate for biomolecular detection.
研究领域:
材料科学
·
生物传感
·
光学
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DOI
高影响因子
OPTICS EXPRESS
·
2018
·Vol. 26
·期 18
·23534-23539
·
IF 3.356
Zhang Jie,Yin Zenghe,Zhang Xiaolei,Zhu Yong
Quantitative surface-enhanced Raman scattering (SERS) in practical applications still remain unresolved, mainly due to low reproducibility relying on the quality of the SERS substrates. In this paper, a carbon nanotube and Ag nanoparticles composite (CNT/AgNPs) is reported, and the carbon nanotube is as an internal standard for the calibration of SERS intensity of analyte molecules. The quantification of analyte molecules rhodamine 6G (R6G) is demonstrated in an aqueous solution with the concentration of 10-9 to 10-7 M. Raman mapping is used to investigate the stability of SERS spectra in a large scanning area, and the corresponding relative standard deviation (RSD) is calculated. SERS mapping reveals that the uniformity of Raman enhancement is improved through the build-in calibration with 2D Raman peak of CNT. Meanwhile, CNT/AgNPs samples are used to detect N2 in natural air, indicating that such self-calibration method can improve the reliability of the SERS analysis.
研究领域:
材料科学
·
分析化学
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高影响因子
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