BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2014
·Vol. 38
·期 5
·833-839
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IF 1.823
Liu Zhenfeng,Shen Yanmin,Li Weiwei,Zhang Wen,Li Tao,Ren Baozeng
The synthesis of d -isoascorbyl stearate from d -isoascorbic acid and stearic acid with immobilized lipase (Novozym ® 435) as catalyst was studied. Response surface methodology and Box-Behnken design with six variables and three levels were employed to evaluate the effects of processing conditions on the conversion of d -isoascorbic acid. The results confirmed that the response surface method and statistical analysis were proved to be useful in developing optimal conditions for d -isoascorbyl stearate synthesis. The optimum conditions were predicted as follows: reaction temperature 48 °C, reaction time 17.7 h, immobilized lipase amount 50.0 % (w/w, of d -isoascorbic acid), substrate molar ratio 9:1 (stearic acid to d -isoascorbic acid), d -isoascorbic acid concentration 0.14 mol/L (based on solvent), 4A molecular sieve addition 200 g/L (based on solvent), and the optimal conversion was 90.6 %. Through the kinetics model fitting of the esterification, it was considered that the esterification conformed to a Ping–Pong bi–bi kinetic model with d -isoascorbic acid inhibition, and the obtained kinetic constants showed that the inhibition of d -isoascorbic acid and the enzyme affinity to substrate were abate with the increase of the reaction temperature.
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Journal of Materials Chemistry A
·
2014
·Vol. 2
·期 25
·9559-9565
·
IF 0
Fei Xue,Zihui Meng,Fengyan Wang,Qiuhong Wang,Min Xue,Zhibin Xu
A novel 2-D photonic crystal (PC) sensing material for the visual detection of glucose with high selectivity at a physiological ionic strength (150 mM) is developed. A monolayer polystyrene crystalline colloidal array (CCA) is embedded in a phenylboronic acid (PBA) functionalized hydrogel film to diffract light to sensitively report on the hydrogel surface area. This 2-D PC sensor is superior to the previously reported 3-D PC sensors due to its fast preparation and simple detection. The binding of glucose would increase the cross-linking of the hydrogel that could shrink the hydrogel to increase the Debye diffraction ring diameter. At a physiological ionic strength of 150 mM, the 2-D PC sensor exhibits significant sensitivity for glucose across the entire human physiological glucose range. Additionally, the 2-D PC sensor shows high selectivity for glucose rather than other sugars (fructose and galactose). The diffraction color change of the 2-D PC sensor can be observed. For validation, the prepared 2-D PC hydrogel sensor is applied for the sensing of glucose in the artificial tear fluid.
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JOURNAL OF MOLECULAR LIQUIDS
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2013
·
IF 1.684
Wenbiao Zhi,Yonghong Hu,Wenge Yang,Yumei Kai,Zheng Cao
The solubility of d -sorbitol was measured in methanol, ethanol, n-propanol, n-butanol, THF, acetone, ethyl acetate and acetonitrile at temperatures ranging from 278.15 to 333.15 K under atmospheric pressure by gravimetric method. The solubility data were correlated with modified Apelblat equation and λh equation which showed that the solubility of d -sorbitol in these organic solvents increased with increasing temperature. The thermodynamic properties of the dissolution process, including enthalpy, entropy and Gibbs energy , were calculated by means of van't Hoff analysis and Gibbs equation. According to the experimental results, ethanol showed to be a relatively better solvent in crystallization process of d -sorbitol.
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Frontiers in Pharmacology
·
2025
·
IF 4.8
Shan He,Yue Wu,Kaixian Lu,Heng Zhu,Xuan Wang,Yaoyao Qin,Huan Li,Lin Zeng,Jiaojiao Han,Xiangyang Zhou,Bin Zhang,Bo Tang
Introduction: Sheep placenta extract (SPE) is a representative traditional medicinal substance that exhibits multiple experimentally validated physiological properties, including anti-aging effects, wound healing acceleration, antioxidant activity, and anti-inflammatory mechanisms. However, the mechanism by which SPE influences the delay of aging is still not yet clear.Methods: Exploring the effects of sheep placenta extract on D-gal induced senescence in a mouse model of aging by macrogenomics and metabolomics.Results: In the serum of aging mice treated with SPE, the levels of antioxidant function such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were notably higher compared to those in the blank group, whereas malondialdehyde (MDA) levels decreased. We revealed that SPE alleviated the changes in gut microbiota caused by aging in mice, with a significant decrease in the Firmicutes/Bacteroidetes (F/B) ratio in the gut. Furthermore, Akkermansia muciniphila (A. muciniphila), which is known for its regulating immune response and potential anti-aging effects, showed a significant increase of 1177.94%. The analysis of UHPLC-QE-MS combined with orthogonal partial least squares discriminant analysis (OPLS-DA) screening of differential metabolites in mouse serum metabolic profiles revealed a significant upregulation of cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and triptolide in serum metabolites, following SPE treatment, which are commonly believed to have immunosuppressive, anti-inflammatory, anti-proliferative, and anti-tumor effects.Discussion: The role of SPE in ameliorating aging may be associated with the increased abundance of A. muciniphila in the gut microbiota and the accumulation of two metabolites, EPA and triptolide, in the serum.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Hexuan Li,Dongdong Li,Yilin Li,Haoran Liu,Junzhi Li,Wei Han
The reaction kinetics of lithium-sulfur (Li-S) battery mainly depends on the capture and catalytic conversion efficiency of lithium polysulfide (LiPSs). The unique electron configuration of the 5d metal and the high electrical conductivity of MXene can enhance the interaction between the catalyst and LiPSs. However, the challenge of enhancing the adsorption of LiPSs by regulating the electronic structure of the catalyst with 5d metal and MXene remains unresolved and obscure. In this work, we have developed molybdenum disulfide (MoS 2 )-tungsten disulfide (WS 2 ) @MXene (MoS 2 -WS 2 @MX) heterostructure catalysts for separator coatings in Li-S batteries by introducing W and MXene for the first time. Theoretical calculations demonstrate that the 5 d metallic and conductive MXene coupling effectively modulate the orbital hybridization degree between the catalyst and LiPSs, thereby reducing the energy barrier for LiPSs conversion. Electrochemical test and in situ tests verify the effectiveness of the strategy on the adsorption and conversion of LiPSs. Consequently, the MoS 2 -WS 2 @MX based batteries exhibit high initial reversible capacity of 950.3 mAh g −1 and low capacity decay of 0.066 % per cycle over 500 cycles at 2 C, which is superior to most reported catalysts. This study provides a dual regulatory strategy for enhancing catalytic activity, offering valuable insights for the advancement of high-performance Li-S batteries.
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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2025
·
IF 4.3
Xinhao Ye,Mijia Li,YunLong Meng,Shiyi Duan,Sijie Zhang,Keyuan Zhong
Cardiac looping is a crucial process during embryonic development. Abnormalities or failures in cardiac looping can lead to congenital heart defects, thereby affecting normal physiological function. Environmental pollutant exposure is one of the major causes of cardiac looping failure. D-tetramethrin is a hygienic insecticide widely used in households and public places, that can enter the human body through contact, insect transmission, and the food chain, thereby impacting human health. In this study, zebrafish embryos were exposed to different concentrations of D-tetramethrin to analyze its effects on heart development, and oxidative stress levels within the embryos. Additionally, qPCR was employed to analyze the transcription and the expression levels of genes related to heart development and function. The results showed that (1) D-tetramethrin exposure significantly reduced heart rate and increased the distance between the sinus venosus and the bulbus arteriosus (SV-BA), which suggested that D-tetramethrin induced cardiac looping failure and led to abnormal heart function. (2) D-tetramethrin exposure elevated the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) in zebrafish embryos while decreasing the enzyme activities of key antioxidant stress enzymes, such as catalase (CAT) and superoxide dismutase (SOD). (3) D-tetramethrin exposure resulted in a significant downregulation of the transcription of cardiac looping-related genes ( Myh6 , Nkx2.5 , Tbx2b , Tbx5a, Tnnt2c and Hand2 ) and heart function-related genes ( Gata4, Vmhc and Nppa ). Our findings indicate that D-tetramethrin causes the accumulation of ROS, which in turn alters the transcription levels of genes related to cardiac looping, ultimately resulting in cardiac looping failure.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2018
·
IF 3.118
Ya-Nan Jia,Hai-Peng Lu,Ya-Lin Peng,Bao-Shun Zhang,Xiao-Bao Gong,Jun Su,You Zhou,Min-Hui Pan,Li Xu
Oxyresveratrol (Oxy) is a natural polyhydroxystilbene abundant in mulberry that has anti-inflammation and anti-oxidant activities. We evaluated the protective effect of Oxy in the context of the lipopolysaccharide and d -galactosamine (LPS/ d -GalN) induced acute liver injury. Oxy restricted the development of histopathological changes, markedly reduced the activity of alanine transaminase (ALT) and aspartate transaminase (AST), which are indicators of impaired liver function. Oxy significantly regulated the contents of oxidative stress related enzymes and products, and inhibited expressions of inflammatory mediators and cytokines. Oxy treatment diminished the Toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) signaling pathway in liver, activated the Kelch-like ECH-associated protein 1(Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2) pathway, and increased expressions of heme oxygenase 1 (HO-1) and quinine oxidoreductase 1(NQO1). Pretreatment with Oxy decreased LPS/ d -GalN stimulated hepatocyte apoptosis by efficaciously raising the B-cell lymphoma 2 (Bcl-2)/Bcl-2 associated X (Bax) ratio, inhibiting the expression and activation of caspases , and activating the phosphoinoside-3-kinase (PI3K)-Akt pathway. Our results demonstrate the hepatoprotective efficacy of Oxy. The protection is mainly due to the prevention of TLR4/NF-κB pathway activation, induced activation of Keap1-Nrf2 signaling pathway, and decreased hepatocyte apoptosis. Oxy warrants further study as a potential therapeutic agent candidate for the management of acute liver injury.
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Science China-Life Sciences
·
2024
·
IF 8
Zhang Yinghui,Liu Yadan,Jiang Bing,Chen Lifan,Hu Jie,Niu Buying,Chang Jie,Fan Zisheng,Zhou Jingyi,Wang Yajie,Teng Dan,Ma Ning,Wang Xiaofeng,Yang Ruirui,Zheng Mingyue,Zhang Sulin
The development of STING inhibitors for the treatment of STING-related inflammatory diseases continues to encounter significant challenges. The activation of STING is a multi-step process that includes binding with cGAMP, self-oligomerization, and translocation from the endoplasmic reticulum to the Golgi apparatus, ultimately inducing the expression of IRF3 and NF-κB-mediated interferons and inflammatory cytokines. It has been demonstrated that disruption of any of these steps can effectively inhibit STING activation. Traditional structure-based drug screening methodologies generally focus on specific binding sites. In this study, a TransformerCPI model based on protein primary sequences and independent of binding sites is employed to identify compounds capable of binding to the STING protein. The natural product Licochalcone D (LicoD) is identified as a potent and selective STING inhibitor. LicoD does not bind to the classical ligand-binding pocket; instead, it covalently modifies the Cys148 residue of STING. This modification inhibits STING oligomerization, consequently suppressing the recruitment of TBK1 and the nuclear translocation of IRF3 and NF-κB. LicoD treatment ameliorates the inflammatory phenotype in Trex1 −1− mice and inhibits the progression of DSS-induced colitis and AOM/DSS-induced colitis-associated colon cancer (CAC). In summary, this study reveals the potential of LicoD in treating STING-driven inflammatory diseases. It also demonstrates the utility of the TransformerCPI model in discovering allosteric compounds beyond the conventional binding pockets.
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JOURNAL OF ECONOMIC ENTOMOLOGY
·
2017
·Vol. 110
·期 4
·1556-1562
·
IF 1.824
Hu Wei,Zhang Ning,Chen Hongli,Zhong Balian,Yang Aixue,Kuang Fan,Ouyang Zhigang,Chun Jiong
Sweet orange oil fractions were prepared by molecular distillation of cold-pressed orange oil from sample A (Citrus sinensis (L.) ‘Hamlin’ from America) and sample B (Citrus sinensis Osbeck ‘Newhall’ from China) respectively, and their fumigant activities against medium workers of red imported fire ants (Solenopsis invicta Buren) were investigated. The volatile composition of the orange oil fractions was identified and quantified using GC–MS. Fractions from sample A (A1, A2, and A3) contained 23, 37, and 48 chemical constituents, and fractions from sample B (B1, B2, and B3) contained 18, 29, and 26 chemical constituents, respectively. Monoterpenes were the most abundant components, accounting for 73.56% to 94.86% of total orange oil fractions, among which D-limonene (65.28–80.18%), β-pinene (1.71–5.58%), 3-carene (0.41–4.01%), β-phellandrene (0.58–2.10%), and linalool (0.31–2.20%) were major constituents. Fumigant bioassay indicated that all orange oil fractions exerted good fumigant toxicity against workers of fire ants at 3, 5, 10, and 20 mg/centrifuge tubes, and B1 had the strongest insecticidal potential, followed by A1, B2, A2, B3, and A3. The fractions composed of more high volatile molecules (A1 and B1) showed greater fumigant effects than others. Compounds linalool and D-limonene, which were the constituents of the orange oil, exhibited excellent fumigant toxicity against red imported fire ant workers. Linalool killed red imported fire ant workers completely at 5, 10, and 20 mg/tube after 8 h of treatment, and D-limonene induced >86% mortality at 8 h of exposure.
研究领域:
昆虫学
·
植物化学
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GOLD BULLETIN
·
2020
·Vol. 53
·期 3
·159-165
·
IF 1.706
Wang Yanwei,Zhou Xiaojuan,Liu Qiang,Jin Yan,Xu Chunli,Li Baoxin
In this work, we proposed one simple and effective chiral-sensing method for the discrimination of carnitine enantiomers. The gold nanorods (AuNRs) were used as colorimetric probes to recognize carnitine enantiomers in this method. l -Carnitine can induce rapidly the blue-to-gray change of AuNR solution color, whereas d -carnitine cannot induce the obvious change of the color. Based on this distinct difference of color, a visual method for recognition of carnitine enantiomers was suggested. This method has been applied to detect the enantiometric excess of l -carnitine in the range of − 100~100%. The presented chiral measurement can be performed with a simple ultraviolet-visible spectrometer and even the naked eyes. In this method, AuNRs need no modification with chiral molecule, and the chiral recognition is based on the inherent chirality of AuNRs. This new method opens up possibility for fast and easy assay of carnitine enantiomers.
Graphical abstract
研究领域:
分析化学
·
生物化学
·
纳米技术
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Lin-Fu Xie,Wan-Hong Huang,Jian-Ping Chen,Hai-Lin Chen,Cheng Hou,Qing-Ling Ni,Ting-Hong Huang,Liu-Cheng Gui,Xiu-Jian Wang
Photocatalytic selective oxidation plays an important role in developing green chemistry. However, it is challenging to design an efficient photocatalyst for controlling the selectivity of photocatalytic oxidation reaction and exploring its detailed mechanism. Here, we synthesized three conjugated microporous polymers (CMPs) with D-A structures, named M−SATE−CMPs (M Zn, Cu and Co), with different d-band centers based on different metal centers, resulting in the discrepancy in adsorption and activation capacities for the reactants, which produces the selectivity of β-keto esters being catalyzed into α-hydroperoxide β-keto esters (ROOH) or to α-hydroxyl β-keto esters (ROH). Density functional theory (DFT) calculations also demonstrate that the adsorption and activation capacities of the metal active centers in M−SATE−CMPs (M Zn, Cu and Co) for ROOH are the key factors to influence the photocatalytic selective oxidation of β-keto ester. This study provides a promising strategy for designing a metallaphotoredox catalyst whose photocatalytic selectivity depends on the d-band center of metal site in the catalyst.
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Journal of Essential Oil Bearing Plants
·
2016
·
IF 0.313
Xin Chao Liu,Shao Liang Liu,Zhi Long Liu
In our screening program for new agrochemicals from wild plants and Chinese medicinal herbs, Teucrium quadrifarium aerial parts at flowering stage was found to possess strong insecticidal activity against booklice (Liposcelis bostrychophila). Water-distilled essential oil from T. quadrifarium aerial parts was analyzed by gas chromatography-mass spectrometry (GC-MS). Fourty-seven components comprising 97.2 % of the total oil were identified and the main components of the essential oil were germacrene D (8.8 %), linalool (8.2 %), camphene (7.8 %), β-caryophyllene (7.3 %), β-cadinene (6.8 %), 1,8-cineole (6.5 %), 4-terpineol (5.8 %), and α-terpineol (5.1 %). The essential oil of T. quadrifarium possessed contact toxicity against L. bostrychophila with a LC50 value of 95.1 μg/cm2. The oil also showed fumigant toxicity against L. bostrychophila with a LC50 value of 222.0 μg/l. These findings suggested that the essential oil of T. quadrifarium aerial parts has potential to be developed as new natural insecticides for the control of grain storage insects.
研究领域:
植物化学
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昆虫学
·
农业科学
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