BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2019
·Vol. 103
·期 1
·193-198
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IF 1.65
Zhang Wenjie,Wang Jun,Wang Jinhua,Zhu Lusheng,Lv Ning,Wang Rui,Ahmad Zulfiqar
The widespread use of pharmaceutical and personal care products (PPCPs) has attracted much attention and the impact of PPCPs on indigenous microbial communities has become increasingly important in recent days. Five common PPCPs, including doxycycline (DOX), ciprofloxacin (CIP), triclocarban (TCC), carbamazepine (CBZ), and sulfadimidine (SMZ), were selected and their effects on soil microbial respiration were studied at concentrations of 0, 0.2, 1, 5, 25 and 50 mg/kg. The results of this study indicate that the effect of five common PPCPs on soil microbial respiration was dose- and time- dependent. At low concentrations (0.2 and 1 mg/kg), CBZ and SMZ exhibited an activation effect on microbial soil respiration at 1 day (58.02%, 26.39% and 1.54%, 1.76% at 0.2 and 1 mg/kg respectively), while DOX showed inhibition for all tested concentrations at 1 day of incubation. At high concentrations (25 and 50 mg/kg) CIP and SMZ showed an inhibitory effect (− 69.13%, − 80.86% for 25 and 50 mg/kg, respectively), while TCC and CBZ exhibited stimulatory effect (38.07%, 9.64% and 4.06%, 12.18% at 25 and 50 mg/kg, respectively) at 1 day of incubation. Our findings indicate that the effect of tested PPCPs on soil microbial respiration had an inhibitory or stimulatory effect based on the dose and extent of time.
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JOURNAL OF POLYMER RESEARCH
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2019
·Vol. 26
·期 6
·1-11
·
IF 1.53
Zhan Yifei,Guan Xinmei,Ren Erhui,Lin Shaojian,Lan Jianwu
In this work, polyacrylonitrile (PAN) nanofibers were utilized to prepare metal-organic framework (MOF) nanofibrous mats. Herein, 2-methylimidazole (2-MI) was mixed in polymeric solution for electrospinning. Subsequently, zeolitic imidazolate framework (ZIF-8) nanocrystals were growth on the surface of PAN nanofibers through post-treatment. To confirm the structures, ZIF-8/PAN nanofibrous mats were investigated by FTIR, XRD, SEM, and TGA measurements. The results presented uniform ZIF-8/PAN nanofibrous mats were fabricated. Further, ZIF-8/PAN nanofibrous mats were employed for dye adsorption using methylene blue (MB) and malachite green (MG) as models. The optimum dye-adsorption conditions were investigated. The results shown the optimum concentration, temperature and pH value of for MB adsorption were 50 mg/L, 30 °C and pH = 11; for MG absorption were 300 mg/L, 30 °C and pH = 5, respectively. Meanwhile, the results displayed prepared ZIF-8/PAN nanofibrous mats presented well adsorption capacity to MG with a quick adsorption rate, which reached to 1531.94 mg/g. Moreover, MB and MG adsorption isotherms of ZIF-8/PAN nanofibrous mats were best fitted by the Langmuir isotherm model, and the results presented pseudo second-order kinetic equation was more consistent with the MB and MG adsorption processes of the adsorbent. Additionally, ZIF-8/PAN nanofibrous mats are successfully degradation MB under xenon light irradiation.
Graphical abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 15
·3361-3372
·
IF 3.286
Zhang Yingyi,Li Liang,Wang Qi,Shen Mei,Han Weili,Yang Xuemei,Chen Lingyun,Ma Ande,Zhou Zhengzheng
Methamphetamine (METH) is one of the most highly addictive illicit drugs abused all over the world. Much evidence indicates that METH abuse leads to major toxicity, medical consequences, and even severe public health consequences. Existing studies usually focus on the pathomechanism of METH-induced toxicity; therefore, data on metabolites and elements correlating with particular toxicity remain scarce. The objective of the present study is to develop appropriate analytical procedures to identify the differential metabolic and elemental biomarkers on METH-induced hepatic injury to rats. The rats were administrated with METH (15 mg/mL/kg, two times per day) via intraperitoneal (i.p.) injections for four consecutive days. The alanine aminotransferase and aspartate aminotransferase activity levels of in the rat serum of the METH group increase significantly compared with those of the control group, suggesting obvious hepatic injury. The results are further confirmed by the histopathological microscopic observation. A total of 18 small molecular metabolites and 19 elements are selected to perform the simultaneous quantification based on the combination of liquid chromatography coupled with tandem mass spectrometry and inductively coupled plasma mass spectrometry. Sample preparation was optimized to cover all the analytes. Both methods are optimized and validated according to developed guidelines such as limits of detection, limits of quantification, linearity, precision, and recovery. All the obtained data are within the satisfactory range. The normalized data were processed according to the partial least squares discrimination analysis (PLS-DA) model. Five differential metabolic and six elemental markers are identified in rat plasma based on the variable importance in projection (VIP) (> 1) and t test results. Overall, the results obtained in this study demonstrate the developed methods are suitable for simultaneous determination of metabolic and elemental markers in the hepatic injury to rats induced by METH.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 9
·3715-3725
·
IF 3.67
Wang Chengcheng,Zheng Pu,Chen Pengcheng
Raspberry ketone is an important ingredient in the flavor and fragrance industries. Due to its low content in fruits and vegetables, the production of natural raspberry ketone using heterologous synthesis in microbial strains is recently attracting increased attention. In this work, a heterologous pathway to produce raspberry ketone from p -coumaric acid, including 4-coumarate: CoA ligase (4CL), benzalacetone synthase (BAS), and raspberry ketone/zingerone synthase (RZS1) from plants, was successfully assembled in Escherichia coli . When the RZS1 gene was introduced into E. coli and co-expressed with two other genes, the intermediate 4-hydroxybenzylidene acetone in the pathway was almost completely transformed into a raspberry ketone. Substituting TB medium for M9 medium increased raspberry ketone titers by 3–4 times. Furthermore, the heterologous pathway was partitioned into two modules; module one produced p -coumaroyl-CoA from p -coumaric acid by 4CL, and module two produced raspberry ketone from coumaroyl-CoA by the action of BAS and RZS1. Optimizing the balanced expression of the two modules, it was shown that moderate expression of module one and high expression of module two was the best combination to enhance raspberry ketone production. The engineered strain CZ-8 reached 90.97 mg/l of raspberry ketone, which was 12 times higher than previously reported. In addition, the preferred approach of the heterologous pathway was related to the heterologous genes from different sources; for example, 4CL from Arabidopsis thaliana seemed to be more suitable for raspberry ketone production than that from Petroselinum crispum . This work paves an alternative way for future economic production of natural raspberry ketone.
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Metabolomics
·
2019
·Vol. 15
·期 4
·1-10
·
IF 3.167
Lu Hengqian,Chen Haiqin,Tang Xin,Yang Qin,Zhang Hao,Chen Yong Q.,Chen Wei
Introduction Metabolomics has been successfully applied to guide the rational engineering of industrial strains and improve the performance of bioprocesses. Mortierella alpina has traditionally been one of the most popular industrial strains for the production of polyunsaturated fatty acids. However, a systematic comparison and optimisation of the metabolomic analysis methods of M. alpina has not yet been reported. Objective We sought to identify potential weaknesses that are important for accurate metabolomic analysis. We also aimed to determine an efficient sample preparation protocol for metabolomics studies in the oleaginous filamentous fungus M. alpina . Methods In this study, using GC-MS, we evaluated three sample preparation protocols and five solvent mixtures by assessment of the metabolite profile differences, the sum of peak intensities and the reproducibility of metabolite quantification. Results The freeze-dried biomass had better reproducibility and recovery than fresh biomass for metabolite extraction and data normalisation that is part of a metabolomics analysis of filamentous fungi M. alpina . Methanol:water (1:1) was superior for the profiling of metabolites in oleaginous fungi M. alpina . The unbiased metabolite profiling difference between the growth phase and lipids synthesis phase revealed that the degradation of amino acids were critical nodes for the efficient synthesis of lipids in M. alpina . Conclusion The use of freeze-dried biomass for metabolite extraction and data normalisation was more efficient at measuring the active state of the intracellular metabolites in M. alpina . We recommend extracting the intracellular metabolites with methanol:water (1:1). An important role of amino acid oxidation in the nitrogen limitation-mediated lipid accumulation was found.
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CATALYSIS LETTERS
·
2019
·Vol. 149
·期 9
·2586-2596
·
IF 2.372
Shen Xiaohua,Wang Jingjing,Liu Miaoqing,Li Mengzhe,Lu Jianjun
Abstract A new route to synthesis hierarchical Ti-rich TS-1 with uniform intracrystalline mesopores, uniform crystal size and the higher Ti content has been developed by using the environmental-friendly and cheap surfactant Triton X-100 as the assisted template agent and (NH 4 ) 2 CO 3 as crystallization-mediating agent. In this way, the surface area and pore volume of the resulting materials can be adjusted by the Triton X-100 content. The catalytic properties of the Nano-size hierarchical Ti-rich TS-1 were investigated by hydroxylation of benzene to phenol with H 2 O 2 as oxidizing agent and deionized water as solvent. Due to the combination of hierarchical pores and high titanium content, the hierarchical Ti-rich TS-1 shows improved catalytic activity in the oxidation of benzene. Graphical Abstract
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Nano Research
·
2019
·Vol. 12
·期 5
·1129-1137
·
IF 8.515
Wang Lingchang,Zhang Chenguang,Jiao Xin,Yuan Zhihao
In the development of wearable energy devices, polypyrrole (PPy) is considered as a promising electrode material owing to its high capacitance and good mechanical flexibility. Herein, we report a PPy-based hybrid structure consisting of vertical PPy nanotube arrays and carbon nano-onions (CNOs) grown on textile for wearable supercapacitors. In this hybrid nanostructure, the vertical PPy nanotubes provide straight and superhighways for electron and ion transport, boosting the energy storage; while the CNOs mainly act as a conductivity retainer for the underlayered PPy film during stretching. A facile template-degrading method is developed for the large-area growth of the PPy-based hybrid nanostructures on the textile through one-step polymerization process. The fabricated stretchable supercapacitor exhibits superior energy storage capacitance with the specific capacitance of 64 F·g −1 . Also, it presents the high capacitance retention of 99% at a strain of 50% after 500 stretching cycles. Furthermore, we demonstrate that the textile-based stretchable supercapacitor device can provide a stable energy storage performance in different wearable situations for practical applications. The use of the PPy-based hybrid nanostructures as the supercapacitor electrode offers a novel structure design and a promising opportunity for wearable power supply in real applications.
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CELLULOSE
·
2019
·Vol. 26
·期 6
·4213-4223
·
IF 3.917
Li Shanshan,Lin Xinghuan,Liu Ying,Li Rong,Ren Xuehong,Huang Tung-Shi
Abstract A novel silicon- and nitrogen-containing compound, poly[3-(5,5-cyanuricacidpropyl)-siloxane-co-trimethyl ammoniumpropyl siloxane chloride] (PCQS) was synthesized and coated onto cotton fabrics with phytic acid (PA) by layer-by-layer assembly. The treated cotton fabrics were characterized by SEM, FT-IR and EDX. The thermal property, mechanical property, flame retardancy and antibacterial property of the coated cotton fabrics were investigated. The breaking strength of the coated cotton fabrics had a small decline. With the flame retardant effect of PCQS and PA, the coated cotton fabrics obtained excellent flame retardancy with LOI value of 29.8% and char length of 8.1 cm. Furthermore, the LOI value and char length of Cotton-PEI/(PCQS/PA) 30 -Cl separately reached 28.5% and 7.9 cm and Cotton-PEI/(PCQS/PA) 30 -Cl also showed high thermal stability with char yield of 34% at 600 °C, whereas pure cotton fabrics only obtained 8% char yield. The Cotton-PEI/(PCQS/PA) 30 -Cl exhibited effective antimicrobial activity against E. coli O157:H7 and S. aureus with 100% bacterial reduction at 10 6 CFU per square inch inoculated level within 1 min of contact time, respectively. Graphical abstract
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Song Xiaolei,Wang Yu,Liu Su,Zhang Xue,Wang Jingfeng,Wang Haiwang,Zhang Fenfen,Yu Jinghua,Huang Jiadong
An amplified electrochemical biosensing scheme is described for lead(II) ions. It is making use of DNAzyme-assisted target recycling and catalytic hairpin assembly (CHA). The hairpin strand (substrate probe for the Pb 2+ -based DNAzyme; referred to as SP) is composed of trigger probe (TP) and a capture probe 1 attached to gold nanoparticles (AuNP). In the presence of the enzyme probe that partially hybridizes with SP, the introduction of Pb 2+ triggers target recycling and drives the highly amplified translation of target Pb(II) to TP. The CHA reaction is further initiated by TP. The modified AuNP act as the connecting unit, and this leads to the formation of a 3D DNA-AuNP network on the electrode (which is the third amplification step). It can bind the positively charged redox mediator RuHex via electrostatic interaction for electrochemical detection. This biosensor has a low detection limit (95 pM) and any analytical range that covers the 100 pM to 5 μM Pb(II) concentration range. It is selective over other divalent metal ions. It was applied to the determination of Pb 2+ in spiked real-world samples.
Schematic presentation of the electrochemical biosensor. The triply amplified electrochemical assay is based on the use of DNAzyme-assisted target recycling with catalytic hairpin assembly (CHA) reaction for sensitive and selective determination of lead ion (Pb 2+ ). AuNP: gold nanoparticles; SP: substrate probe; EP: enzyme probe.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2019
·Vol. 125
·期 8
·1-15
·
IF 1.784
Yang Lu,Sun Zhuangzhi,Li Fei,Du Shanqi,Song Wenlong
To enhance the performance of ionic actuators, the electrochemical and electromechanical properties of green ionic actuators with the electrolyte layer after doping with MWCNT was investigated in this paper. The surface morphology, elemental composition, corresponding content and functional group of the mixed electrolyte layer were observed and characterized by SEM–EDS, FT-IR and XRD. The CV test showed that when the doping amount was 0.8 wt%, the specific capacitance increases 187.6% and 205.3% compared with undoped electrolyte layer at the sweep speed of 20 mV s −1 ,500 mV s −1 , respectively. The GCD test showed that the energy density increased to 144.3% at the current density of 1 A g −1 . and the cycle life is correspondingly increased. At 5 V DC, the deflection displacement of actuators was 10.12 mm when the doping amount was 0.8 wt%, which was 238.1% higher than when the doping amount was 1.5 wt%. And the output force of actuators was 3.10 mN, which was 147.2% higher than when the doping amount was 0 wt%. At 5 V/0.05 Hz voltage, the peak displacement of the actuator was 2.871 mm, which was 238.1% higher than when the doping amount was 1.5 wt%.
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CELLULOSE
·
2019
·Vol. 26
·期 13
·8153-8164
·
IF 3.917
Yang Maiping,Liu Weiqu,Jiang Chi,Xie Yankun,Shi Hongyi,Zhang Fengyuan
Abstract Self-cleaning, photocatalysis and UV shielding cotton fabric was fabricated through a mild, facile and efficient method via ZnO/polydimethylsiloxane coatings. No fluorinated materials were employed during the preparation process. The modified cotton presented a high superhydrophobicity, with a water contact angle of 160.4° and water shedding angle of 6.0°. The SEM, EDS and XPS tests were used to investigate the differences of microstructures and chemical compositions of fabrics before and after coating. The robustness of the coated fabric was demonstrated in many aspects, such as immersing in acid and basic solutions, different organic solvents and abrasion test. In addition, the as-prepared superhydrophobic surface displayed excellent self-cleaning due to the synthesis function of superhydrophobicity and photocatalysis. The UV durability and UV shielding characteristics were also investigated. These properties promote the multifunctional cotton fabric as a satisfied candidate in various promising fields. Graphic abstract
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2019
·Vol. 139
·期 3
·1931-1939
·
IF 2.471
Qu Dezhi,Gao Hongwei,Wang Qiuxia,Bai Yongping,Li Ning
Crystallization kinetics are very important for polymeric materials due to the fact that they determine the final degree of crystallinity and the morphology of the final product after processing. The kinetic of non-isothermal crystallization of poly(butylene-co-isosorbide succinate) (PBIS) was investigated using differential scanning calorimetry. Ozawa, Jeziorny and Mo methods were used to describe the non-isothermal crystallization process of PBIS. Both the Ozawa constant and the Avrami constant were obtained by Ozawa method and Jeziorny method, respectively. The crystallization activation energy (∆ E ) for non-isothermal crystallization process of PBIS, analyze based on the differential isoconversional method of Friedman and Kissinger, was found to increase with the relative crystallinity.
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