ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Gao Meiyu,Wu Yu,Yin Huihui,Wang Qiang,Tian Yuan,Zhang Zunjian,Xu Fengguo,Zhang Pei
Phosphatidyl amino acids ( p -AAs) are metabolites characterized by the phosphorylation of the hydroxyl, amino, carboxyl, and thiol groups of amino acids. Previous research has primarily focused on the phosphorylation sites within macromolecular proteins, with a particular emphasis on typical O- p -AAs. In this study, we established a prediction library of p -AAs based on existing knowledge. To improve detection rates of p -AAs in biological samples, we employed a chemical labeling-based LC–MS/MS method, utilizing p -[3,5-(dimethylamino)-2,4,6-triazine] benzene-1-sulfonyl piperazine (Tmt-PP) and its deuterated form (d12-Tmt-PP) as paired labeling reagents. A preliminary identification was performed by matching characteristic MS fragments with available standards. Additionally, strategies such as in vitro methods were implemented for further identification. The phosphatase treatment aids in identifying phosphate-modified metabolites by dephosphorylating them, while cell extract incubation helps determine if novel phosphorylated amino acids are generated in vivo. Ultimately, we identified 11 p -AAs, 6 of which are novel metabolites reported for the first time. A pseudotargeted metabolomics method covering 11 identified p -AAs was established and applied to investigate the differences between cisplatin-resistant non-small cell lung cancer (NSCLC) cells and their parental cells, as well as their derived exosomes. This approach enhances our understanding of the role of p -AAs in various health and disease conditions and contributes to the discovery of additional novel phosphatidyl metabolites. Graphical
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Guo Lan,Zhao Dong-Mei,Hu Bao-Hua,Wu Shuo,Chen Shuai,Yu Yong-Liang
The detection of the illegal additive azodicarbonamide (ADA) in flour and related products is crucially important due to the harmful effects of excessive consumption on human health. However, the requirement for expensive instruments and trained operators poses a major challenge for on-site testing of ADA, particularly in field enforcement and consumer self-monitoring. Herein, an economical and portable 3D-printed photothermal (PT) device constructed by a 3D-printed sample bracket, a separable 96-well plate, an 808 nm laser, and a thermometer was rationally designed for quantitative on-site testing of ADA in flour. Since the target ADA could convert reducing glutathione (GSH) into non-reducing glutathione disulfide (GSSG), manganese dioxide nanosheets (MnO 2 NSs) in this sensing system remain intact to catalyze the conversion of dopamine (DA) into polydopamine nanoparticles (PDA NPs). During the detection process, the PT signal changes induced in the sample driven by the 808 nm laser could be easily recorded with a digital thermometer. The proposed PT method demonstrates an excellent linear response for ADA in the range of 30–1000 μM, with a detection limit as low as 10 μM, which was significantly lower than the maximum allowable ADA level in flour extracts (388 μM). This portable and cost-effective PT sensing system shows great potential for quantitative on-site testing, enabling immediate food safety identification in resource-limited regions. Graphical
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PLANT MOLECULAR BIOLOGY
·
2025
·Vol. 115
·期 4
·1-21
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IF 3.8
Xu Yao,Li En,Cao Weiping,Zhang Yingchao,Zhang Xinfang,Liu Zhaoyu,Cai Shanshan,Wang Jiayu,Cheng Fansheng,Chen Ruibing,Gao Ting
Scutellaria baicalensis , a traditional medicinal plant originating in China, is widely cultivated for its therapeutic properties. The main bioactive substances in S. baicalensis are flavonoids, which exhibit extensive antibacterial and antiviral activities. However, the contents of these valuable natural product ingredients are relatively low in the plant. MYB transcription factors play crucial roles in regulating plant secondary metabolism, including flavonoid biosynthesis. While the regulation of MYB transcription factors has been extensively studied in various species, research on their role in S. baicalensis remains relatively scarce. In this study, we identified SbMYB111, belonging to the S7 subgroup of R2R3-MYB transcription factors, which functions as a transcriptional activator and is localized in the nucleus. Through heterologous overexpression of SbMYB111 in Arabidopsis thaliana and suppression expression in S. baicalensis , we demonstrated that SbMYB111 acts as a positive regulator in the biosynthesis of flavonoids. Furthermore, the yeast one-hybrid and dual-luciferase reporter gene assays validated that SbMYB111 activates the expression of SbC4H2 , a key enzyme gene in the flavonoid biosynthesis pathway. This study provides a theoretical basis for understanding the transcriptional regulation mechanism of flavonoid synthesis and further developing medicinal resources of S. baicalensis .
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RESEARCH ON CHEMICAL INTERMEDIATES
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2025
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IF 3.5
Hussain Asif,Ahmed Sadam,Boota M.,Khan Pir Bukhsh,Nazir Sadia,Jeridi Mouna,Alshahrani Thamraa,Hou Jianhua
Advanced industrialization and globalization have conduct to extensive energy and pollution emergencies, demanding the growth for novel solutions. The release of synthetic dyes as industrial waste has emerged as a global environmental challenge. Large quantities of these dyes are discharged into wastewater each year, posing serious risks due to their toxic and harmful effects. Semiconductor’s materials have emerged as a sustainable and green remediation solution for contaminant degradation. Among various materials, graphitic carbon nitride stands out as an extensively investigated material due to ease fabricated and low cost. In this study, sulphur-incorporated graphitic carbon nitride was fabricated for photocatalytic. In this study, sulphur-assisted graphitic carbon nitride was fabricated for photocatalytic activity. Several fabrication routes were employed, including thermal polymerization, sonication, chemical oxidation, and stepwise thermal polymerization. The material was characterized, and its photocatalytic activity was evaluated for methylene blue dye degradation. The fabricated trials exhibited 2.79 eV, 2.72 eV, 2.82 eV, and 2.56 eV band gap energies, with, 67%, 70%, 75%, and 99.5% pollutants degradation activity, respectively. The trial fabricated with chemically oxidation route attributed higher photocatalytic activity (99.5%, in 50 min) with the reaction rate constant ( K = 0.069). The aforementioned results and measurements are supported by UV–vis diffuse reflectance, signifying bandgap energy, XRD for structural and SEM for morphological and EDX for elemental qualitative analysis. The chemical oxidation route shows an effective method for the synthesis of sulphur-assisted graphitic carbon nitride. This novel approach has the potential to advance fabrication techniques within the scientific community. Graphical abstract
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JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
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2025
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IF 3.5
Liang Yongchao,Huo Hongjin,Wei Wei,Wang Dongxuan,Lu Jingjing,Yi Wenjie,Jiang Fei
Purpose Perfluorooctane sulfonic acid (PFOS) is a representative persistent organic pollutant that has been shown to impair male reproductive function. Epidemiological studies and animal models suggest that vitamin D may modify male reproductive function. However, whether 1α,25(OH) 2 D 3 could mitigate the PFOS-induced damage of Sertoli cells, and the underlying mechanisms remain poorly defined. Methods Mouse Sertoli cell line TM4 were pretreated with or without 10 nM or 100 nM 1α,25(OH) 2 D 3 for 6 h, followed by 100 µM or 200 µM PFOS for 24 h. Cell viability was assessed using the CCK8 assay. Reactive oxygen species (ROS) were detected by fluorescent staining, while malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) levels were measured using commercial kits. siRNA transfection, quantitative real-time PCR (qRT‒PCR), and Western blotting were performed to elucidate the underlying mechanisms. Molecular docking studies were conducted to explore the potential binding of PFOS to Keap1. Results PFOS increased MDA contents, decreased GSH levels, and induced cytotoxicity in mouse Sertoli cells. Mechanistically, PFOS might directly bind to Keap1, facilitating its degradation, leading to persistent activation of the Nrf2-ARE pathway. Conversely, 1α,25(OH) 2 D 3 attenuated PFOS-induced cytotoxicity by alleviating the Nrf2-ARE pathway, a protective effect which was abolished by vitamin D receptor (VDR) knockdown. Conclusion PFOS induces cytotoxicity in Sertoli cells by binding to Keap1 and persistently activating the Nrf2-ARE pathway. 1α,25(OH) 2 D 3 emerges as a promising candidate for mitigating PFOS-induced testicular dysfunction. Graphical abstract
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Nature Communications
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2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Pan Zhenxiang,Lei Yalong,Yan Tiange,Zheng Fuxin,Liao Yu,Zhan Jiang,Zhang Tong,Shao Lu,Han Gang
Membrane nanofiltration (NF) has emerged as a prominent technology for efficient separations of ions, but state-of-the-art polyamide (PA) NF membranes are constrained by a pernicious tradeoff between water permeance and selectivity. This work conceives a versatile molecular engineering strategy to simultaneously improve water permeance and co-cation selectivity through molecular construction of cationic triazolyl heterocyclic polyamide (CTHP) nanofilms via scalable interfacial polymerization. Experimental data and molecular simulations reveal that the CTHP structures precisely regulate the subnanometer pore architecture and binding affinity with water and ions, affording advanced size-sieving and Donnan exclusion while facilitating water partitioning and transport. The exemplified PA membrane exhibits ultrahigh divalent cation rejections of over 99% with a 9-fold increase in monovalent/divalent cation sieving selectivity relative to the pristine benchmark, exceptional water permeance, and good fouling resistance. The implemented molecular engineering strategy holds broad prospects for the rational design of high-performance polymeric membranes for sustainable and precision separations.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-17
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IF 15.7
Deng Ruotong,Chen Wei,Zhu Hanjie,Li Yuxi,Ou Yining,Wang Jian,Ruan Qing,Zhang Xuanying,Zhang Junbo,Zhang Yongxian,Niu Yantao,Hu Zhubin,Zhang Chunlei,Xu Huaping,Cao Wei
The escalating utilization of ionizing radiation across medicine and industry underscored the paramount urgency of effective radioprotective materials. Conventional materials such as lead and concrete are widely used, and lead-free materials have also emerged to solve the problems of cumbersome and toxic lead, such as metal-containing micro/nano materials and polymers. Nevertheless, there is still a significant challenge in meeting the urgent need for lightweight and biocompatible alternatives. To tackle this challenge, this work utilizes molecular engineering of melanin to develop a panel of metal-free melanin materials with enhanced conjugation, heightened physical shielding against radiation and effective antioxidant properties. Furthermore, engineered melanin materials demonstrated in vivo γ-ray protection, increasing mice survival from ~12% to 100% after 6 Gy total body irradiation.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-16
·
IF 15.7
Zhang Zekun,Guo Hua,Li Shiji,Gao Suihan,Wang Xinyu,Li Mingtao,Yan Wei,Xu Hao
The regulation of product selectivity in electrochemical CO 2 reduction (ECO 2 R) remains fundamentally constrained by the dynamic equilibrium between intermediate transport and surface coverage. In this study, we report a progress in catalytic architecture through precision-engineered Au@Cu 2 O yolk-shell tandem nanoreactors featuring dual-tunable parameters: cavity confinement dimensions and shell thickness gradients. This structural modulation enables dynamic control over both *CO intermediate enrichment and reaction pathway bifurcation. ECO 2 R performance evaluations demonstrate significant product selectivity switching at −1.31 V (vs. reversible hydrogen electrode (RHE)). The Faradaic efficiency (FE) for CH 4 exhibits significant architectural dependence, increasing from 43.02% (thick-shell/large-cavity) to 65.54% (medium-dimension) and then decreasing to 23.26% (thin-shell/small-cavity). Conversely, the FE for C 2 H 4 demonstrates an inverse structural correlation, improving from 6.68% (medium-dimension) to 38.73% (thin-shell/small-cavity). The spatial domain-limiting mechanism of the yolk-shell structure directly controls the transition between protonation-dominated CH 4 formation and coupling-driven C 2 H 4 production. This work establishes a pioneering paradigm for dynamically steering catalytic selectivity through purely geometrical modulation, bypassing traditional compositional tuning limitations, thereby opening avenues for precision design of advanced electrocatalytic systems.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Li Haobo,Zhu Yicheng,Zhao Zihan,Ma Ruixin,Lu Jiachen,Wan Wenjie,Chen Qianli
Ionic transport in solids is a critical process for energy devices including batteries and fuel cells. To improve ionic transport, an emerging approach is the selective excitation of atomic vibrations related to the mobile ions. However, there is limited direct experimental evidence demonstrating enhanced macroscopic ionic conductivity through this approach. Here, we use a 140 mW continuous-wave mid-infrared (MIR) light to excite the O–H stretch vibration in proton-conducting yttrium-doped barium zirconate. We observe reversible enhancement of 36.8% in bulk, and 53.0% in grain boundary proton conductivities, controlled by MIR irradiation. Decreases in the activation energy and prefactor for bulk proton conduction suggest possible reduction in activation entropy and attempt frequency of proton hopping. We rationalize the enhancement as the excitation of O–H stretch vibrational states, followed by the relaxation into lattice vibration modes, modulating the potential energy surface of the proton. Our findings highlight MIR irradiation as a power-saving strategy to optimize the performance and operation cost of solid-state electrochemical devices by selective modulation of the vibrational properties.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-13
·
IF 15.7
Xu Linsheng,Qin Tian,Li Yuanfeng,Guo Hao,Hu Qiao,Xiong Jing,Ma Yaxiao,Zhang Peng,Yu Xiaolin,Liu Xi,Liu Yunpeng,Zhao Zhen,Zou Jianping,Wei Yuechang
Simultaneous oxidation of soot and CH 4 emitted from natural gas-diesel dual fuel engine is a new challenge. Herein, a robust catalyst of binary Ru-Pd components supported on three-dimensional ordered macro-mesoporous cerium-zirconium oxide (RuPd/3DOMM-CZO) is elaborately constructed. Ordered macro-mesopore structure in novel hierarchical porous cerium-zirconium oxide can respectively enhance mass transfer of soot and gaseous reactants, and binary Ru-Pd active components can improve activation for NO and CH 4 . RuPd/3DOMM-CZO catalyst exhibits excellent catalytic performance and stability during simultaneous soot and CH 4 oxidation. Based on the results of characterizations and theoretical calculations, Ru site in binary Ru-Pd components is responsible for catalyzing NO oxidation to NO 2 , which is key step of NO x -assisted soot oxidation mechanism, while Pd site can activate the C-H bond of CH 4 for boosting oxidation to CO 2 ; the catalytic cooperation between Ru and Pd atoms can promote the rate-determining step to realize simultaneous oxidation of soot and CH 4 .
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JOURNAL OF MATERIALS SCIENCE
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2025
·
IF 3.9
Zhong Tian,He Guoxu,Wan Menghui,Xu Chenglong,Wang Zhiqiang,Wang Zhihua,Zhao Yanbao,Sun Lei
Inspired by the multi-scale wrinkled patterns observed on tree bark surfaces, a novel approach was developed to enhance oil–water selectivity in emulsion separation by constructing nanoscale rough structures on membrane surfaces, endowing them with superhydrophobic and superoleophilic properties. Specifically, PVDF electrospun nanofibrous membranes were coated with a composite layer composed of polydimethylsiloxane (PDMS) and TiO 2 nanoparticles through a simple one-step dip-coating method, resulting in the formation of PVDF@PDMS/TiO 2 membranes featuring heterogeneous interfaces. Through heat treatment at 120 °C, a durable three-dimensional textured surface was fabricated, exhibiting a water contact angle of 170.2°, which confirms its remarkable superhydrophobic properties. The membrane demonstrated outstanding performance in oil–water separation, achieving a separation efficiency greater than 99%, a high flux of 1100 L m⁻ 2 h⁻ 1 , and effective removal of organic dyes through the photocatalytic activity of TiO 2 . Antibacterial testing demonstrated over 99% inhibition effectiveness against both Staphylococcus aureus and Escherichia coli . Moreover, the membrane retained a contact angle above 160° when exposure to strong acidic or alkaline conditions, indicating excellent chemical stability. These characteristics suggest that the developed multifunctional nanofibrous membrane with a hierarchical wrinkled structure exhibits significant potential for the efficient treatment of complex industrial oily wastewater systems. Graphical abstract
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BMC MICROBIOLOGY
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2025
·Vol. 25
·期 1
·1-12
·
IF 4.2
Yang Manli,Zuo Shanningmei,Liu Ahui,Zhong Nana,Lu Xueping,Liu Xun,Jiang Xiasen,Hu Tao,Liu Yuntong,Ren Xiaogang,Zhou Kang,Wu Chuansheng
Background Bisphenol A (BPA), a widespread environmental pollutant, has been extensively studied for its effects on bacteria and plant, but its impact on rhizosphere bacterial communities and plant root traits is less understood. At the same time, the role of bacteria in helping plants resist adversity is widely recognized, but the relationship between BPA-induced with rhizosphere bacterial changes and root development is still unclear. Therefore, this study investigated the effects of varying BPA concentrations (1.5, 17.2, and 50 mg/L) on soybean root traits and rhizosphere bacterial communities, as well as the relationship between them. Result The results revealed that BPA exposure significantly altered root traits, with root length, surface area, volume, and tip numbers being suppressed at 50 mg/L, while lower concentrations (1.5 and 17.2 mg/L) promoted root elongation and thickening. Bacterial community composition shifted notably, with Bacillota increasing and Pseudomonadota decreasing in relative abundance across all BPA treatments. Alpha diversity, measured by richness and Shannon_e indices, increased slightly at lower BPA concentrations, while beta diversity (Bray_Curtis and UniFrac) analysis showed significant differences, particularly at 50 mg/L. Community assembly processes (βNRI and βNTI) were dominated by deterministic mechanisms at lower BPA concentrations but shifted toward stochastic processes at 50 mg/L. Correlation analysis revealed significant relationships between bacterial community dynamics and root traits (Principal component PC1 and PC2), with alpha diversity indices influencing root traits represented by PC2 and beta diversity indices showing a negative correlation with PC1. Conclusions BPA exposure not only alters root morphology and bacterial community structure but also highlights the intricate interplay between rhizosphere bacteria and plant roots under BPA stress. This study contributes to the theoretical understanding of plant–microbe interactions in contaminated environments and may inform future research on microbial involvement in plant stress responses.
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