Food Science and Technology
·
2022
·
IF 2.602
FENG Yingying,NAN Haijuan,ZHOU Haoyu,XI Penghang,LI Bo
Bavachalcone is an important active component of the traditional Chinese medicine Fructus Psoraleae. The inhibitory effect of bavachalcone on α-glucosidase activity is reported for the first time and the mechanism elucidated by molecular docking. The inhibition of α-glucosidase by bavachalcone (IC50 15.35 ± 0.57 μg/mL) was significantly superior to acarbose (IC50 2.77 ± 0.09 mg/mL). Inhibition type was mixed competitive and non-competitive. Molecular docking suggested this inhibition stems from hydrogen bonds formed with the trp391, arg428, and trp710 residues of α-glucosidase.
研究领域:
药理学
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分子生物学
·
中药学
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2024
·Vol. 35
·期 8
·1-12
·
IF 2.8
Wang Junjun,Xue Lin,Zhang Lei,Ruan Zhen,Hua Wanren
In this work, Sn-loaded α -Fe 2 O 3 nanosheets were made using a straightforward solvothermal technique and utilized for acetone detection. The materials’ structural and chemical characteristics were evaluated using XRD, SEM, BET, and XPS. By varying the quantity of Sn, various constitutions could be created, and the associated sensing mechanism was addressed. The sample containing the 3 at.% Sn has good sensing performance. Acetone responds at a lower working temperature (225 °C), reaching 6.7–100 ppm. It has a splendid response time (5 s) and a sensor with better repeatability and stability. All of these phenomena suggest that Sn improves both the surface’s ability to adsorb sensitive materials and its sensitivity to the target gas. Sn will enhance the surface area specifically and promote the adsorption and redox reaction of the gas. As a result, the sensor offers significant possibilities for acetone detection.
研究领域:
材料科学
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传感器
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纳米技术
·
化学检测
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Fang Pan,Yueping Li,Yujie Ding,Songwei Lv,Rongrong You,Roja Hadianamrei,Mhd Anas Tomeh,Xiubo Zhao
Anticancer peptides (ACPs) have attracted increasing attention in cancer therapy due to their unique mechanism of action on cancer cells. The main challenge is to establish the correlation between their physicochemical properties and their selectivity and anticancer effect, leading to a clear design strategy. In this study, a series of new α-helical short peptides (coded At1-At12) with different anticancer activities were systematically designed with different amphiphilicity based on a natural α-helical antimicrobial peptide (AMP) derived from ant. Three of the designed peptides, At7, At10 and At11, showed considerable anticancer activity with low toxicity to normal skin fibroblasts. The high selectivity of the peptides is attributed to their balanced amphiphilicity and cationic nature which favours binding to the outer membrane of negatively charged cancer cells over the neutral membrane of normal mammalian cells . In addition to rapid membrane penetration, the designed peptides also damaged the mitochondria and induced mitochondrial membrane depolarization. Moreover, these peptides were found to induce apoptosis in cancer cells by up-regulating the expression of apoptotic proteins Bax and Caspase-3, down-regulating the apoptotic protein Bcl-2, and activating the Caspase enzyme-linked reaction. The results of this study reveal the potential of these peptides for clinical applications, and provide a guidance for further development of highly selective anticancer medications.
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PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2023
·
IF 5.1
Honghui Liu,Puxin Huang,Xingchen Wang,Yufang Ma,Jing Tong,Jing Li,Hong Ding
This study investigated the inhibitory potential of a series of synthesized compounds (L1-L27) on α-glucosidase. Among them, compound L22 showed significant inhibitory effect. Through enzymatic kinetics studies, we demonstrated that L22 acts via a non-competitive inhibition mode with a K i value of 2.61 μM, highlighting its high affinity for the enzyme. Molecular docking studies revealed the formation of hydrogen bonds between L22 and α-glucosidase and diverse interactions with neighboring amino acid residues. Furthermore, molecular dynamics simulations confirmed the stability of the L22-α-glucosidase complex. In a mouse model of type 2 diabetes, treatment with L22 significantly lowered fasting blood glucose levels, and reduced insulin resistance, suggesting its potential as a therapeutic agent for type 2 diabetes. Furthermore, L22 showed a protective effect against oxidative stress in the liver and alleviated liver and pancreatic abnormalities. These results provide valuable insights into the mechanism of action of L22 and its potential applications to treat type 2 diabetes, and improve liver health.
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PubMed
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POLYMER
·
2026
·
IF 5.1
Zhiqi Lin,Wenjie Zhou,Zijian Yang,Chao Chen,Yihan Qiu,Yiyun Cheng,Mingquan Chen,Jingjing Hu
Fungal infections remain a major health burden due to limited treatment options, biofilm-associated tolerance, and increasing emergence of drug-resistant strains. Antifungal polymers have gained growing interest because their molecular structures can be rationally tuned to optimize antifungal activity. Herein, we prepared a series of side-chain-functionalized oligo(α-lipoic acid)s and identified the primary amine-bearing oligomer OLA-EDA as the lead candidate, based on its favorable balance between antifungal activity and cytocompatibility. Mechanistic assays showed that OLA-EDA induced relatively limited fungal membrane depolarization and permeabilization, but markedly increased intracellular reactive oxygen species (ROS) levels. NAC pretreatment partially rescued fungal viability, supporting the involvement of oxidative stress or redox imbalance in the OLA-EDA-mediated antifungal activity. In addition, OLA-EDA inhibited hyphal development and biofilm formation and downregulated related virulence-associated genes. In murine vulvovaginal candidiasis model, OLA-EDA treatment reduced fungal burden and alleviated local inflammation, indicating its potential for topical antifungal therapy.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 1
·67-77
·
IF 3.713
Mengqing Zhang,Ruijin Yang,Shuhuai Yu,Wei Zhao
Summary α-Glucosidase inhibitors, which can inhibit α-glucosidase to reduce the release of glucose after a mixed carbohydrate diet, has become a key tool in the management of type 2 diabetes mellitus (T2DM). SFP-1, an α-glucosidase inhibitor derived from Sargassum fusiforme ( S . fusiforme ), was studied as a food-derived hypoglycaemic agent. By binding α-glucosidase and then changing the conformation of the enzyme, SFP-1 with a mixed inhibition type exerted an excellent inhibitory effect (IC 50 value: 0.681 mg mL −1 ). Structural analyses suggested that the average molecular weight of SFP-1 was 1.61 × 10 5 Da, mainly composed of glucuronic acid, fucose, galactose, glucose, mannose and xylose, and the relative molar ratio was 23.07: 15.17: 7.72: 4.92: 4.04: 1.99. FTIR and NMR analysis suggested that SFP-1 was an acidic polysaccharide with both α-type and β-type glycosidic bonds. S . fusiforme polysaccharide (SFP-1) has the potential to develop as functional foods and nutraceuticals in T2DM management.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2018
·Vol. 55
·期 9
·3640-3646
·
IF 1.797
Lou Wenjuan,Chen Yongsheng,Ma Hanjun,Liang Guizhao,Liu Benguo
Tannic acid widely exists in plants, which forms a part of human diet. The antioxidant activity of tannic acid was evaluated by the chemical and cellular antioxidant assays. And its α-amylase inhibitory activity and behavior were also investigated. It was found that hydrogen- and electron donating capacities of tannic acid were higher than those of tertiary butylhydroquinone (TBHQ) based on reducing power, ABTS and DPPH radical scavenging assays. But for its low hydrophobic property, the antioxidant activity of tannic acid in linoleic acid system was inferior to that of TBHQ. In the cellular antioxidant assay, tannic acid showed the higher activity than gallic acid in the “PBS wash” protocol, which could attribute to its high binding capacity of cell membrane. Compared with acarbose, tannic acid possessed the stronger α-amylase inhibitory capacity. And the static fluorescence quenching of α-amylase in the presence of tannic acid could be also observed, which was caused by their binding interaction.
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PubMed
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JOURNAL OF APPLIED PHYSICS
·
2014
·Vol. 115
·期 17
·
IF 2.185
Li S. J.,Li D.,Jiang J. J.,Liu G. B.,Ma S.,Liu W.,Zhang Z. D.
Growth process and magnetic properties of PbO-type α-Fe x Se nanostructures with shape changing from nanocacti to nanopetals and then to nanosheets are investigated. With iron acetylacetonate [Fe(acac) 3 ] and Se powder as raw materials, the diffusion process of Fe atoms dominates the synthesis of α-Fe x Se nanocacti following phase transitions from FeSe 2 to Fe 3 Se 4 and finally to α-Fe x Se. When a mixed solution containing Se precursor and Fe(acac) 3 was used as the raw material, the formation of FeSe 2 and Fe 3 Se 4 can be avoided and, bended α-Fe x Se nanopetals can be prepared at 345 °C, which became flat nanosheets with a [001] preferred orientation as extending the reaction time from 1 to 4 h. No superconducting transition occurs in the α-Fe x Se (0.84 ≤ x ≤ 1.05) nanostructures due to composition heterogeneity or size effect. Magnetic measurements indicate that an antiferromagnetic component with a Néel point at about 45 K dominates the magnetic properties of the α-Fe 0.87 Se nanosheets.
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APPLIED SURFACE SCIENCE
·
2014
·
IF 2.538
Chang Su,Changbai Liu,Li Liu,Mucui Ni,Haiying Li,Xiaoqing Bo,Lili Liu,Xiao Chi
Pure and 3 wt% Sm-doped α-Fe 2 O 3 nanotubes are prepared via a typical electrospinning technology and calcination procedure. The characteristic and morphology of the synthesized nanotubes are investigated by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). Compared with pure α-Fe 2 O 3 nanotubes, 3 wt% Sm-doped α-Fe 2 O 3 nanotubes display enhanced gas-sensing properties. The response of 3 wt% Sm-doped α-Fe 2 O 3 nanotubes is 33–50 ppm acetone, which is 13 times larger than that of pure α-Fe 2 O 3 nanotubes. The lowest detection limit of acetone is 500 ppb to which the response is 2.3 at the optimum operating temperature of 240 °C. The response and recovery times are about 6 and 11 s to 50 ppm acetone, respectively. In addition, 3 wt% Sm-doped α-Fe 2 O 3 nanotubes exhibit good selectivity and long term stability.
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STARCH-STARKE
·
2025
·
IF 2.7
Zixuan Lin,Tingting Hu,Lin Lin,Tao Li,Yan Yan,Wei Wei,Dongzhi Wei
Although α-amylases are critical biocatalysts in starch processing, fungal α-amylase diversity remains largely untapped. This study presents the first systematic characterization of an α-amylase from Penicillium brevicompactum NRRL864. Maximum α-amylase activity of 195 U/mL was obtained at pH 6.0 and 55°C under optimized fermentation conditions. These conditions consisted of 0.75% starch, 3% peptone, 0.5% NaCl, incubation at 20°C for 7 days. A novel α-amylase gene (DF) was identified via phylogenetic analysis and heterologously expressed in Escherichia coli . The gene encodes a 551-amino-acid α-amylase with an open reading frame of 1656 bp, showing 68.36% sequence identity to the α-amylase from Penicillium expansum . The DF enzyme displayed a specific activity of 489.4 U/mg under optimal conditions of 50°C and pH 6.0, and a 124.2% activity enhancement with 1 mM Ca 2 ⁺, indicating Ca 2 ⁺-dependence. The activity of DF was inhibited by a range of chemical reagents, including EDTA and urea. Substrate specificity assays confirmed that the DF enzyme exhibited optimal activity in hydrolyzing soluble starch. DF-mediated saccharification of corn starch yielded a reducing sugar concentration of 317.9 µg/mL within 70 min, confirmed by thin-layer chromatography. Beyond identifying P. brevicompactum as a novel α-amylase source, this work demonstrates DF as a Ca 2 ⁺-responsive GH13 enzyme with significant potential for industrial applications in biofuel and sweetener production.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Yage Sun,Xinrui Zhao,Rong Yang,Meng Xiao,Wenguang Liu
Anti-epilepsy drugs (AEDs), a common therapeutic approach for clinically treating epilepsy, suffer from low permeability toward the blood-brain barrier (BBB), side effect risks, and a failure to regulate the pathological microenvironment. These issues hinder the complete control of epilepsy and lead to a vicious cycle of inflammation-epileptogenesis. Herein, an α-lipoic acid (LA)-based nanozyme that loads phenytoin (PHT) and complexes calcium ions (LA@PHT-Ca 2+ ) is developed. This nanozyme combines the functions of delivering AEDs across the BBB and regulating undesirable microenvironments to treat epilepsy. Amphipathic LA self-assembles into stable nanoparticles in water with BBB crossing ability endowed by the transporting of LA through sodium-dependent multivitamin transporter. LA's enzyme-like and redox functions provide LA@PHT-Ca 2+ with excellent scavenging effects on several free radicals via cascade reactions, thereby protecting neurons from oxidative stress. Moreover, Ca 2+ disrupts neuronal respiration, resulting in reduced production of bioenergetic adenosine triphosphate and an improved hypoxic microenvironment. This leads to decreased expression of P-glycoprotein, a drug-efflux gating protein. This novel lipoic acid-based nanozyme, with intrinsic BBB crossing capability, prolonged intracellular drug retention time, and the ability to block the inflammation-epileptogenesis cycle, presents a satisfactory therapeutic effect in epileptic rats, resulting in attenuated symptoms and an improved microenvironment.
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高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-9
·
IF 17.694
Cao Peisheng,Zheng Haoyue,Wu Peng
Designing organic fluorescent and phosphorescent materials based on various core fluorophore has gained great attention, but it is unclear whether similar luminescent units exist for inorganic materials. Inspired by the BX 6 octahedral structure of luminescent metal halide perovskites (MHP), here we propose that the BX 6 octahedron may be a core structure for luminescent inorganic materials. In this regard, excitation-dependent color-tunable phosphorescence is discovered from α-AlF 3 featuring AlF 6 octahedron. Through further exploration of the BX 6 unit by altering the dimension and changing the center metal (B) and ligand (X), luminescence from KAlF 4 , (NH 4 ) 3 AlF 6 , AlCl 3 , Al(OH) 3 , Ga 2 O 3 , InCl 3 , and CdCl 2 are also discovered. The phosphorescence of α-AlF 3 can be ascribed to clusterization-triggered emission, i.e., weak through space interaction of the n electrons of F atoms bring close proximity in the AlF 6 octahedra (inter/intra). These discoveries will deepen the understanding and contribute to further development of BX 6 octahedron-based luminescent materials.
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