SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiu Qin,Chunling Yuan,Guoxing Geng,Rui Shi,Shiqi Cheng,Yilin Wang
An enzyme-free colorimetric method for sensitive detection of uric acid (UA) was established, which was achieved by inhibiting the etching of gold nanorods (AuNRs). The longitudinal direction of AuNRs would be gradually etched by I 2 , as an oxidant, generated by the reaction between MnO 2 nanosheets (MnO 2 NS) and I − . Benefitted from the length change, the localized surface plasmon resonance (LSPR) peak of AuNRs showed an obvious blue shift, led to the color change of AuNRs solution. Once UA was introduced, MnO 2 NS was reduced to Mn 2+ rapidly. As a result, the formation of I 2 was reduced and the etching of AuNRs was inhibited. Therefore, a simple method for the assay of UA was achieved with UV–vis absorption spectra and bare eyes, respectively. In optimization conditions, LSPR absorption peak shift (Δλ) of AuNRs before and after inhibition enabled the sensitive detection of UA in the range of 0.8−30 μM and 30−300 μM, the detection limit of low concentration range was 0.76 μM. The developed method has been utilized for determining UA in clinical samples with satisfactory results.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2021
·
IF 3.599
Qutong Zheng,Li Xiong,Long Yu,Di Wu,Chunxu Yang,Yuxiu Xiao
An enzyme-free fluorescent sensing platform based on lanthanide metal-organic framework (Eu-BDC, BDC:1,4-benzenedicarboxylic acid) doped electrospinning fiber membrane (Eu-BDC@FM) was established for the detection of uric acid (UA) in human urine. The Eu-BDC@FM has Eu 3+ characteristic emission, excellent fluorescence stability in pH 4.5–8.0, appropriate hydrophilicity and good reusability (at least 5 cycles). The fluorescence intensity of Eu-BDC@FM can be quenched by UA through inner filter effect . The relationship between the quenching efficiency and UA concentration [A] accords with Stern-Volmer formula in the range of 0–200 μM: I 0 /I = 0.0106 [A]+1.0199 (r = 0.9933), where I 0 and I are the fluorescence intensity of Eu-BDC@FM immersed in water and the UA aqueous solution respectively. The quenching constant is 10.6 × 10 3 M −1 and the limit of detection is 0.6 μM. The Eu-BDC@FM-based fluorescent sensing platform was applied to detect UA in real urine samples. The common substances in human urine have no interference with UA sensing, and the recovery is in the range of 95–110% with relative standard deviation less than 2.0%. The proposed method is simple, robust, inexpensive, and has good selectivity , accuracy and precision for quantification of UA in real urine samples.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Ming Yang,Xiaoning Ren,Taotao Yang,Chao Xu,Yongqin Ye,Zhaowei Sun,Linghao Kong,Bin Wang,Zhiqiang Luo
Conductive hydrogels, a class of novel polymeric material that combine the electronic conductivity of intrinsically conductive materials and diverse functionalities of hydrogels, are promising candidates for a range of applications in bioelectronics. However, poor conductivity and biocompatibility of conventional conducting hydrogels compromise the reliable function of hydrogel bioelectronic devices. Herein, we report the development of a conjugated polypyrrole (PPy) hydrogel with conductive sulfonated multi-walled carbon nanotubes (s-MWCNTs) as crosslinking agent. The electrostatic interaction between -SO 3 - groups of s-MWCNTs and nitrogen groups of PPy facilitates the hydrogel gelation, and the conductivity of PPy/s-MWCNTs hydrogel with interconnected 3D conducting network is measured to be 30 S m −1 . Furthermore, the in vitro cytocompatibility results suggest that the proposed PPy/s-MWCNTs hydrogels have excellent biocompatibility. For bioelectronics applications, the PPy/s-MWCNTs hydrogels were demonstrated to serve as highly sensitive electrochemical biosensors for in situ detection of biomolecules released from living cells and cell-substrate impedance biosensors for real-time monitoring of cell proliferation. Our strategy for assembling conjugated polymer hydrogel with conductive crosslinker would offer new insight for the construction of highly conductive hydrogels for various potential applications in bioelectronics.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Zhifeng Cai,Shulin Pang,Liangliang Wu,Erxiao Hao,Jiaxin Rong
In this contribution, dopamine-protected copper nanoclusters as a novel fluorescent nanosensor was employed to detect nitrofurantoin (NFT) for the first time, which were prepared by using dopamine as the stabilizing agent and sodium borohydride (NaBH 4 ) and hydrazine hydrate (N 2 H 4 ·H 2 O) as the reducing agents. A series of methods were used to analyze the structure and optical properties of as-prepared Cu NCs, such as UV-Vis absorption spectroscopy, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). It possessed high dispersion, excellent water solubility, good photostability and strong green fluorescence under UV lamp (365 nm). Significantly, the FL intensities of Cu NCs were quenched with addition of NFT. The analytical method possessed good linear relationship between the relative fluorescence intensity (F 0 /F) and the NFT concentrations (range from 5 to 120 μM), and the limit of detection (LOD) could reach 0.73 μM. The fluorescence detection mechanisms were attributed to the static quenching and inner filter effect (IFE). In addition, this proposed fluorescence sensor has been successfully used for the detection of NFT in bovine serum samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 14
·6490-6501
·
IF 5.396
Ao Sun,Xiaoqin Hu,Huijian Chen,Yulan Ma,Xiyue Yan,Dangsheng Peng,Jie Ping,Youe Yan
Ursolic acid (UA) shows an effect on obesity and related metabolic diseases, but its mechanism of action remains unclear. We found that UA clearly reduced the body weight and adipose tissue mass and improved the glucose tolerance and insulin sensitivity in obese male mice. UA treatment significantly reduced the volume and weights of the epididymal white adipose tissue (eWAT) and inguinal subcutaneous white adipose tissue (igSWAT) of HFD-fed mice, respectively. UA also decreased the expression of genes involved in adipocyte differentiation and lipogenesis in igSWAT. Real-time PCR and immunohistochemistry showed that the expression of beiging-related genes 4-1BB factor (CD137), T-box transcription factor 1 (TBX1), and transmembrane protein 26 (TMEM26) were significantly increased in the UA treatment group. UA treatment significantly reduced the weight of gastrocnemius muscle (GM) and lipid droplets in the GM. UA treatment significantly upregulated the expression of PR domain-containing 16 (PRDM16), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), and fibronectin type 3 domain-containing protein 5 (FNDC5) in GM and igSWAT. UA also stimulated irisin secretion in the serum. In conclusion, these results indicate that UA plays an anti-obesogenic role by increasing the secretion of irisin and promoting the beiging of WAT.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 16
·4255-4265
·
IF 4.157
Li Qian,Tian Aixiang,Chen Cuiying,Jiao Tiying,Wang Ting,Zhu Shengyu,Sha Jingquan
Anderson-type polyoxometalate containing Fe 3+ and Mo 6+ , (NH 4 ) 3 [H 6 Fe(III)Mo 6 O 24 ] (FeMo 6 ), was found to work as an oxidase-mimicking nanoenzyme for the first time, exhibiting the ability of catalytic oxidation of o -phenylenediamine (OPD), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTs), and 3,3′,5,5′-tetramethylbenzidine (TMB), which features easy synthesis, low cost, simple operation, and low consumption. Attributed to the nature of FeMo 6 and Fenton-like effect, a novel sensor based on two consecutive “turn on” fluorescence was developed for detecting dopamine (DA) by employing the FeMo 6 -OPD system, and the linear range was from 1 to 100 μ M with the detection limit 0.0227 μ M (3σ/s). Moreover, to increase oxidase-mimic activity of FeMo 6 , reduced graphene oxide (rGO) loading FeMo 6 composites (FeMo 6 @rGO (n), n = 5%, 10%, 15%) was fabricated, and results show that oxidase-like activities of FeMo 6 @rGO (n) are dependent on the mass ratio of FeMo 6 /rGO, and FeMo 6 @rGO (10%) exhibits the highest oxidase-mimic activity and the fastest respond time (4 min) among all reported oxidase mimic of DA to date.
Anderson-type Mo-POMs FeMo 6 was found to work as an oxidase-mimicking nanoenzyme for the first time and was used to detect DA for two consecutive “turn on” fluorescence sensor modes
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Li Liu,Li Wang,Qisheng Liang,Tao Guo,Feng Guo
Copper oxide-single walled carbon nanotube (CuO-SWCNT) hybrid nanostructure and poly-(diallyldimethylammonium chloride) (PDDA) modified glassy carbon electrode (GCE) for sensitive enzyme-free H 2 O 2 detection has been fabricated via electrostatic interaction strategies. Characterization by XRD, IR and SEM showing that CuO was coated on SWCNT molecular wires, and positively charged PDDA attached to the negatively charged SWCNT, thus CuO-SWCNT-PDDA was constructed. The reduction peak current of H 2 O 2 was significantly increased due to the catalytic property of CuO, which was further enhanced by the synergic effect of SWCNT due to its improvement of electron transfer rate. The calibration curve was linear in the range of 1–1150 µM with a detection limit of 0.39 µM. The electrode coupled with amperometric measurement was successfully applied in H 2 O 2 residue determination in milk and the soaking solutions of chicken paw samples. The results have no remarkable difference compared with the reference UV–Vis method.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Hao Lin Zou,Ling Yun Qin,Hong Qun Luo,Bang Lin Li,Nian Bing Li
Semiconducting MoS 2 obtained from the liquid-phase exfoliation is oxidized at the potentials higher than 0.65 V via applied electrochemical processes, and high-valence Mo(VI) are formed. The oxidized MoS 2 exhibits comparable performance to the electrochemical oxidation of nitrite, promoting the construction of sensors based on the assembly of nanomaterials. The signal amplification mechanism is exploited, and enhanced sensing responses are confirmed considering the strong coupling effect of Mo(VI) to nitrite. In order to optimize the sensing performance, the measurement parameters including the pH of buffer solutions, scan rates, and modification conditions are discussed. The cyclic voltammetry and chronoamperometry are successfully employed, and the oxidized MoS 2 performs satisfactory linear relationship to nitrite concentrations ranging from 1.0 μM to 386.0 μM, with the detection limit of 0.028 μM. The oxidation state and enhanced electrochemical responses of semiconducting MoS 2 as well as the developed nitrite sensing strategy inspire emerging characteristics of layered nanostructures and achieve the high potential in practical environmental and food safety monitoring.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 3
·1-9
·
IF 5.833
Xian Zhiquan,Zhang Li,Yu Ying,Lin Bixia,Wang Yumin,Guo Manli,Cao Yujuan
A nanozyme based on CoFe 2 O 4 modified with MoS 2 was constructed for colorimetric determination of cysteine (Cys) and glutathione (GSH). Firstly, ferrite CoFe 2 O 4 is synthesized, and it is then modified by MoS 2 to form a flower-like polymer (MoS 2 @CoFe 2 O 4 ). In the presence of H 2 O 2 , a redox interaction takes place, and the resulting hydroxyl promoted a colorimetric conversion from colorless to blue in the presence of 3,3′,5,5′-tetramethylbenzidine (TMB). However, once Cys or GSH is added, they are capable to compete with the interaction of the hydroxyl with TMB, resulting in an inhibition of the colorimetric conversion. The colorimetric distinction is sensitive to the amount of target. The results obtained proved that the catalytic efficiency of MoS 2 @CoFe 2 O 4 is 4.4-fold and 1.8-fold to that of MoS 2 and CoFe 2 O 4 . Meanwhile, the K m values to TMB and H 2 O 2 are 0.067 and 0.048 mM, respectively, which are 6.5-fold and 77-fold, respectively smaller than those of natural peroxidase such as HPR. This indicates that the MoS 2 @CoFe 2 O 4 possesses a favorable interaction affinity. Additionally, the colorimetric distinction caused by the competition between TMB and cysteine or glutathione is obvious. The signal responses to cysteine and glutathione are linear in the range 0.5~15 μM and 0.5~35 μM, and the LODs are 0.10 and 0.21 μM, respectively. In practical assay of Cys in serum, the RSD of the sample tests is 4.6%, and the recoveries for the spiked assays are 95.3% and 96.0% with the RSD of 2.1% and 4.2%, respectively. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zhihao Xu,Qinzhi Wang,Hui Zhangsun,Shuang Zhao,Yijian Zhao,Li Wang
In this work, we propose a electrochemical enzyme-free glucose sensor by direct growth of conductive Ni/Co bimetal MOF on carbon cloth [Ni/Co(HHTP)MOF/CC] via a facile hydrothermal method. Due to excellent conductivity between Ni/Co(HHTP)MOF and CC, synergic catalytic effect of Ni and Co elements, the Ni/Co(HHTP)MOF/CC not only provides larger surface area and more effective active sites, but also boosts the charge transports and electro-catalytic performance. Under optimized conditions, the Ni/Co(HHTP)MOF/CC shows excellent activity with a linear range of 0.3 μM–2.312 mM, a low detection limit of 100 nM (S/N = 3), a fast response time of 2 s and a high sensitivity of 3250 μA mM −1 cm −2 . Furthermore, the Ni/Co(HHTP)MOF/CC was successfully applied for the detection of glucose in real serum and beverages with competitive performances. This facile and cost-effective method provides a novel strategy for monitoring of glucose in biological and food samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 8
·2135-2146
·
IF 4.157
Mei He,Ma Yange,Wu Huimin,Wang Xuedong
Herein, we synthesized and characterized glutathione-capped copper nanoclusters (CuNCs) using a convenient one-pot chemical reduction approach based on glutathione as capping and reducing agents. The Ce(III) induced aggregation-induced emission of CuNCs to form a CuNCs-Ce 3+ fluoroprobe due to electrostatic and coordination interactions between Ce 3+ and CuNCs. In contrast to CuNCs, the fluorescent intensities (FLs) of CuNCs-Ce 3+ were enhanced by ~ 40-fold concomitant with 20-nm blue-shift of the maximum emission, and a 3.45-fold lengthening of the average fluorescent lifetime. The FLs of CuNCs-Ce 3+ were selectively quenched at 650 nm by hydrogen peroxide (H 2 O 2 ) via the redox reaction. Based on this phenomenon, the sensitive assay of H 2 O 2 was realized, and the linear range spanned over the range of 14–140 μM. Notably, the visualization of the fluorescence quenched effect of H 2 O 2 could be easily attained. Additionally, glucose could be specifically oxidized by glucose oxidase to produce H 2 O 2 , and thus the detection of glucose was achieved according to changes in the concentrations of H 2 O 2 . Under optimized conditions, the fluorescent assay of glucose based on the CuNCs-Ce 3+ system offered the linear range of 8–48 μM with detection limit of 2.4 μM. Meanwhile, high selectivity of the as-constructed fluorescent assay allows the sensitive detection of H 2 O 2 and glucose in real-world care products and human serum samples, showing a great application potential in their conventional monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yang Zhao,Wei Zhao,Hong-Yuan Chen,Jing-Juan Xu
Nanoparticle plasmon scattering can provide real-time imaging information on the formation process of noble metal-based nanomaterials . Due to the synergistic effect of the interface between metal and oxide supporting pores, metal nanoparticles (NPs), especially Au NPs, generally exhibit higher catalytic activity on oxide carriers than single-component NPs. Here, we use the dark field scattering microscope to in situ monitor the growth of Au on Cu 2 O surface by oxidation-reduction reactions and the nanostructures could be precisely controlled via the scattering signal. The prepared Cu 2 O/Au nanocomposite has a higher electrocatalytic activity toward Glucose. When being used as a potential biosensor for nonenzyme glucose detection, excellent performance, such as high sensitivity with a detection limit of 4 μM, high selectivity and outstanding stability, was obtained. The scattering imaging strategy is a convenient and universal approach in controllable synthesis of plasmonic heterostructures, and leads to the improvement of electrocatalysts in biosensing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）