PHARMAZIE
·
2018
·Vol. 73
·期 9
·503-507
·
IF 1.016
Liu Yixian,Wang Yafeng,Peng Youmei,Liu Bingjie,Ma Fang,Jiang Jinhua,Wang Qingduan,Chang Junbiao
The purpose of the present study was to dig into recent studies designed to characterize the impacts of 2'-deoxy-2'-β-fluoro-4'-azidocytidine (FNC) on P-glycoprotein (P-gp), multidrug resistance-associated protein 2 (MRP2) and breast cancer resistance protein (BCRP). Specifically,
the modulation effects of FNC on P-gp, MRP2 and BCRP protein expressions were assessed by western blot methods. 5 (and 6)-Carboxy-2',7'-dichloroflourescein (CDF) and BODIPY-prazosin were used to provide indications of alterations of MRP2 and BCRP activities. The effects of P-gp, MRP2 and BCRP
on FNC were evaluated in the in situ single-pass intestinal perfusion model. The results showed that FNC at higher concentrations and with longer incubation times can upregulate the protein expression of P-gp, MRP2 and BCRP in Caco-2 cells. The upregulated proteins were also functionally
active, as revealed by a lower degree of CDF and BODIPY-prazosin uptake by the cell monolayers. The intestinal absorptive coefficient (Peff) was observed to significantly increase with the inhibitors of P-gp, MRP2 and BCRP. These results suggested that FNC could modulate the expressions and
functions of P-gp, MRP2 and BCRP, while P-gp, MRP2 and BCRP were involved in the efflux transport of FNC. The inductive effects of FNC on P-gp, MRP2 and BCRP suggested the possibility of FNC to contribute to the inter- and intra-individual variability of itself, as well as to alter the absorption
of other drugs that may be administered concomitantly.
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JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
·
2016
·Vol. 43
·期 5
·585-593
·
IF 2.745
Jin Li-Qun,Guo Dong-Jing,Li Zong-Tong,Liu Zhi-Qiang,Zheng Yu-Guo
In this paper, a simple and effective method using sodium metasilicate as precursor and amine as additive was first reported to immobilize recombinant nitrilase, for efficient production of 2-hydroxy-4-(methylthio) butanoic acid from 2-hydroxy-4-(methylthio) butanenitrile. High immobilization recovery of enzyme activity (above 90 %) was achieved. The immobilized enzyme displayed better thermal stability, pH stability and shelf life compared to free nitrilase. Moreover, it showed excellent reusability and could be recycled up to 16 batches without significant loss in activity. 200 mM 2-hydroxy-4-(methylthio) butanenitrile was completely converted by the immobilized enzyme within 30 min, and the accumulation amount of 2-hydroxy-4-(methylthio) butanoic acid reached 130 mmol/g of immobilized beads after 16 batches. These encouraging results demonstrated the efficiency of the new technology for nitrilase immobilization, which has great potential in preparation of 2-hydroxy-4-(methylthio) butanoic acid.
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ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Wenfen Zhang,Yanhao Zhang,Qiong Jiang,Wenjie Zhao,Ajuan Yu,Hong Chang,Ximei Lu,Fuwei Xie,Baoxian Ye,Shusheng Zhang
To satisfy the requirement of simultaneous extraction and characterization of diverse kinds of multitarget analytes, the preparation, characterization, and application of a novel tetraazacalix[2]arene[2]triazine (TCT) coated magnetic nanoparticle (TCT MNP) adsorbent are presented in this paper. TCT assembles two benzene rings and two triazines with nitrogen cross-bridging links, which exhibits a unique structural framework and versatile recognition features based on the multiple recognition sites. These include π electron stacking, charge transfer, hydrogen bonding, and ion-exchange. TCT MNPs acted as a dispersive SPE adsorbent showing strong interaction with and adsorption of polycyclic aromatic hydrocarbons (PAHs), nitroaromatics, and heavy metal ions. The dispersive magnetic nanoparticle solid phase extraction (d-MNSPE) strategy with the simultaneous extraction and stepwise elution (SESE) procedure was designed and optimized for the five PAHs (phenanthrene, anthracene, pyrene, chrysene, and benzo(a)pyrene), six nitroaromatics (4-nitrotoluene, 2,4-dinitrotoluene, 2,4,6-trinitrotoluene, 4-nitrophenol, 2,4-dinitrophenol, and 2,4,6-trinitrophenol), and four metal ions (Cu, Zn, Mn, Cd) in aqueous samples. Due to the high stability, desirable durability, larger saturation magnetization, reuse and distinct enrichment capacity of TCT MNPs, the d-MNSPE method with the SESE strategy provided high recovery (>90%) and good precision (relative standard deviations, RSD < 10%). Coupled with the commonly used HPLC-fluorescence detection, HPLC-UV detection, and atomic absorption spectrometry, these trace probes in tap water, river water, and lake water were determined with very low detection limits, in the range of 0.09–0.15 pg mL–1 for PAHs, 6–11 pg mL–1 for nitroaromatics, and 17–53 pg mL–1 for metal ions after being enriched by the d-MNSPE. The determination of trace PAHs in urine samples from smokers and nonsmokers was successfully carried out with this method, which implied that the versatile TCT MNPs and the robust method together represent a significant potential application in the analysis of body fluids and disease markers. Such methods for accurate quantification of trace components in water are very valuable as they fulfill an unmet need in environmental and medicinal chemistry.
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JOURNAL OF RARE EARTHS
·
2024
·
IF 5.2
Shuangshuang Ding,Yongbiao Yan,Jianwei Zhou,Yangyang Hua,Hongxing Cai,Shuang Li
In this work, the upconversion luminescence (UCL) and temperature-sensing properties of SrGd 2 O 4 phosphors doped with Er 3+ , Yb 3+ were investigated. The UCL performance was studied by adjusting the doping concentrations of Er 3+ , Yb 3+ . It can be found that the intensity of red UCL is effectively improved by doping Yb 3+ ions. The band structures of SrGd 2 O 4 , SrGd 2 O 4 :5 mol%Er 3+ , and SrGd 2 O 4 :5 mol%Er 3+ ,15 mol%Yb 3+ were calculated using the density functional theory (DFT) to verify the experimental results. The thermal sensitivity of SrGd 2 O 4 :5 mol%Er 3+ ,15 mol%Yb 3+ phosphors was studied using the novel fluorescence intensity ratio of non-thermally coupled energy levels (NTCL-FIR) technique based on the 2 H 11/2 and 4 F 9/2 energy levels. The analytical results reveal that the maximum values of S r are 1.69%/K (300 K), 1.18%/K (300 K) and 1.47%/K (300 K) respectively under 915, 980 and 1550 nm excitations. The S r values of novel NTCL-FIR are compared with that of thermal coupling of energy level (TCL) and other studies in the same field. The results show that the thermometry sensitivity of red-emitting UCL materials can be enhanced by using the novel NTCL-FIR technique. In addition, the intense red UCL of SrGd 2 O 4 :Er 3+ ,Yb 3+ phosphors have the advantage of deeply penetrating biological tissues. Therefore, the combination of novel NTCL-FIR and red-emitting SrGd 2 O 4 :Er 3+ ,Yb 3+ phosphors could be conducive to temperature monitoring in vivo .
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JOURNAL OF MOLECULAR STRUCTURE
·
2011
·
IF 1.599
Xian-Lan Hong,Hong Li,Cui-Hong Peng
A new Ru(II) complex [Ru(dmb) 2 (pdta)](ClO 4 ) 2 ( 1 ) (dmb = 4,4′-dimethyl-2,2′-bipyridine, pdta = 3-(pyridine-2-yl)-as-triazino [5,6-f]-acenaphthylene) was synthesized and characterized by elemental analysis (EA), ES–MS, 1 H NMR, UV–Vis and CV. The DNA-binding properties of complex 1 were investigated by absorption titration, thermal denaturation , viscosity measurement and photoactivated cleavage. The DNA-binding constant was determined to be 2.37 ± 0.14 × 10 5 M −1 ( s = 1.90 ± 0.04) by electronic absorption titration, indicating that complex 1 interacts with DNA through intercalative mode. The redox peak potentials of this complex in CV were rationally assigned with the frontier molecular orbital stereographs resulting from the density functional theory (DFT) calculation. The photoactivated cleavage and cleavage mechanism of plasmid pBR322 DNA in the presence of complex 1 and various inhibitors were studied. The experiment for detecting the formation of singlet oxygen photo-sensitized by complex 1 was carried out, and the antioxidant activity of the Ru-complex and the ligand pdta against hydroxyl radical was also investigated.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Jun Zhang,Xiu-tian-feng E,Bo Yang,Xiumei Ma,Xueping Wang,Ninghui Chang,Qiuyu Zhang,Ji-Jun Zou,Junjian Xie
High-energy-density cyclobutane fuels were directly synthesized via room-temperature-integrated one-pot two-step oxidation/self-photosensitized [2 + 2] cycloaddition using lignocellulose-derived cyclic ketones and cyclic alcohols for the first time, which endow much higher density, lower freezing point, and higher neat heat of combustion than other biofuels achieved from the same feedstocks and hence denote a new kind of sustainable high-density fuels appealing for practical applications.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Liuxing She,Lei Peng,Hang Yuan,Tianrong Zhu,Qiang Fu,Zaikun Wu
The absorption kinetics of CO2 into a novel nonaqueous mixture of 2-amino-2-methyl-1-propanol (AMP) and piperazine (PZ) was studied at atmospheric pressure. The effects of AMP/PZ molar ratio, total concentration, absorption temperature, and inlet flow rate were discussed. The results showed that the composite absorbent with an AMP/PZ ratio of 6:4 had a good effect on the CO2 absorption at 293 K. Unlike relevant reports at home and abroad, the solubility of CO2 in the 1-pentanol solution of AMP and PZ (AMP-PZ-Pentanol) was calculated based on a solution rule model and relevant data from Aspen Plus. The absorption kinetics of CO2 into nonaqueous composite absorbent was analyzed by a double stirred-cell absorber. It shows the enhancement factor increases with the increasing of total concentration but decreases with the increasing of absorption temperature. The absorption mechanism of CO2 in AMP-PZ-Pentanol was determined to be the formation of carbamate by FT-IR, 13C NMR, and XRD analysis. Based on the vapor–liquid equilibrium theory, the reaction heat of CO2 into the phase change absorbent was calculated to be 9.30 KJ mol–1. The results show that the composite absorbent has a lower reaction heat than any single component in it. The desorption experiment of saturated solution shows that the composite absorbent can be completely regenerated in a short period of time, which meets the industrial requirements for CO2 absorbents and will provide a theoretical basis for further industrial applications of this type of absorbent.
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ANALYTICAL LETTERS
·
2024
·
IF 1.6
Dapeng Sun,Chenglong Ye,Zhuo Chen
Matrix metalloproteinase-2 (MMP-2) is a key biomarker involved in various pathological processes, so its sensitive and selective detection is of great clinical significance. A fluorescent sensor for the detection of MMP-2 was successfully designed based on the inner filter effect (IFE) between carbon dots (CDs) and gold nanoparticles (AuNPs). In the presence of MMP-2, peptide chains are hydrolyzed, releasing short peptides that contain thiol groups and positive charges, thereby inducing the aggregation of AuNPs. This aggregation disrupts the IFE between AuNPs and CDs, leading to a significant enhancement of the fluorescence. By monitoring the change in fluorescence intensity of CDs, the concentration of MMP-2 was accurately determined. The sensor exhibited a linear response across the concentration range from 1.0 to 150 ng/mL, with a detection limit as low as 0.23 ng/mL, which is lower than those of previously reported methods. Furthermore, the sensor demonstrated minimal interference from coexisting biochemical substances for MMP-2 in human serum. Notably, the sensor was successfully applied to determine MMP-2 in human serum, showing satisfactory recovery rates ranging from 98.8 to 1106.0%. In conclusion, this fluorescent sensor offers high selectivity and sensitivity for MMP-2 detection, making it a promising tool for clinical diagnosis of related diseases.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2020
·
IF 2.69
Ye Han Yan,Muhammad Atif,Ren Yong Liu,Hai Kun Zhu,Li Juan Chen
In this work, the comb-like poly(2-methyl-2-oxazoline) copolymer, poly(2-aminoethyl methacrylate-random-poly(2-methyl-2-oxazoline) (PMOXA-r-AEMA, PMA) is synthesized, and the CuSO4/H2O2-triggered dopamine/PMA co-deposition process is investigated. Ellipsometry, water contact angle (WCA), and X-ray photoelectron spectroscopy (XPS) are used to characterize the thickness, hydrophilicity, and surface composition of PMA-based coatings. PMA is facilely anchored to substrates within 60 min with the assistance of dopamine/polydopamine triggered by CuSO4/H2O2, and the coating thickness can achieve about 13 nm. Anti-protein adsorption and anti-blood platelet adhesion measurements are also studied to verify their antifouling properties. The adsorption capacity of FITC-BSA can be reduced to 5% of the original surface, and PMA-based coatings present excellent protein-resistant properties (∼95% reduction relative to gold surface) according to surface plasmon resonance (SPR) measurement, and it can resist adhesion of almost all blood platelets. Moreover, it is applied to a capillary inner wall for surface modification. An alkaline protein mixture and egg white proteins are successfully separated, and present excellent stability. The relative standard deviation (RSD) of migration time is lower than 0.71%. The practicability of this promising PMA-based coatings with this preparation strategy can be used for further proteomics analysis.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zimin Cai,Fangqin Fu,Sifeng Zhu,Jibin Wu,Xu Han,Shuai Jiang,Zhiyong Qian,Cheng Wang
The pharmacokinetic behavior of nanocarrier in vivo has been confirmed to be greatly influenced by the dynamic protein corona covered its surface, which also affects the ultimate clinical efficacy. Herein, we explored one novel polymersomes based on Poly(2-methyl-2-oxazoline) polymers (PMOXA 5 -PDBH 13 -PMOXA 5 ) with excellent drug-carrying capacity and specific protein corona manipulation. Compared to PEGylated nanoparticle (NP), the polymersomes (PC-SOM) provided a considerable advancement in therapeutic efficiency, which can selectively accumulate in tumor tissues, enhancing cellular internalization via specific proteins covering its surface such as fibronectin and moesin. Notably, in vivo antitumor effect showed that doxorubicin-loaded PC-SOM exhibited superior inhibitory rate 75.5% than 48.24% of PEGylated NP. Furthermore, through protein corona analysis we found that PC-SOM enhanced the adsorption of apolipoprotein A-I while suppressed the adsorption of complement C3 and immunoglobulin in serum from healthy volunteers and human breast cancer volunteers. These elaborated the reasons of longer circulation times and superior tumor-targeting capabilities of PC-SOM. Our work provided a promising nanoagent with novel surface-design by the dynamic protein corona for excellent pharmacokinetic behavior and antitumor effect.
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MINERALS ENGINEERING
·
2025
·
IF 5
Jiaqiao Yuan,Xiang Gong,Hongyu Lu,Wei Jiang,Hao Lai,Shuming Wen,Shaojun Bai,Dandan Wu
To address the challenge of separating ilmenite from titanaugite, the feasibility of a novel collector (2-ethylhexyl) phosphonic acid mono-2-ethylhexyl ester (HEHEHP) was firstly investigated in this work. The flotation results indicated that HEHEHP possessed superior collecting performance over a broader pH range for ilmenite compared to benzohydroxamic acid and sodium oleate. Under conditions with a pH of 8.0±0.2, a high-quality concentrate with a TiO 2 grade of 40.74% and a recovery of 87.66% was achieved. Adsorption studies and AFM analysis implied that the adsorption density of HEHEHP on ilmenite was significantly higher than that on titanaugite. Zeta potential analysis implied that the negatively charged HEHEHP anion primarily acted on ilmenite through chemisorption. ToF-SIMS, XPS, and FTIR further corroborated that HEHEHP interacted with the Fe and Ti sites on the ilmenite surface primarily through the P=O and P-OH functional groups in the adsorption process, leading to the formation of PO-Fe or PO-Ti bonds, thereby chemically adsorbing onto the ilmenite. DFT calculations proved that the two O atoms in the PO(OH) group exhibited significant reactivity, serving as the main active sites during the adsorption of HEHEHP. In summary, the chemical interactions between the PO(OH) group in HEHEHP and the active Fe and Ti sites on the ilmenite surface are responsible for the primary mechanism for the selective adsorption of HEHEHP.
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Desalination and Water Treatment
·
2015
·
IF 1.173
Ben Zhang,Juan Li,Xiaogai Lv,Yuanchen Cui,Ying Xu
Polyaspartic acid/2-amino-2-methyl-1,3-propanediol graft copolymer, a novel non-phosphorus scale and corrosion inhibitor, was synthesized and characterized by infrared spectroscopy. Its performance was evaluated by static scale inhibition method and dynamic corrosion testing. It was found that the graft copolymer showed excellent inhibition ability against CaCO3, CaSO4, and Ca3(PO4)2 scales and a definite inhibition corrosion performance as well. The inhibition efficiency was close to 99.9% against CaCO3, CaSO4, and Ca3(PO4)2 when the inhibitor was at an appropriate concentration. The inhibitor had still possessed higher antiscale efficiency against CaCO3 with increasing concentration of Ca2+ and heating time. In addition, The graft copolymer could change the structure of crystals CaCO3, CaSO4, and Ca3(PO4)2 by observation of scanning electron microscopy.
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