FOOD CHEMISTRY
·
2025
·
IF 9.8
Wenze Li,Weichen You,Xiaofan Yang,Yi Wang,Rimao Hua,Saraschandra Naraginti,Meiqing Zhu
As a typical chiral organophosphate insecticide, the enantiomer of profenofos exhibits significant bioselective toxicity in the environment. This study investigates the enantioselective effects of chiral profenofos and 2-Bromo-4-chlorophenol on BSA. Multispectral analysis confirmed that S/R -profenofos and 2-Bromo-4-chlorophenol alterd the microenvironment of BSA, decreasing the α-helices content from 53.6 % to 52.9 %, 53.0 %, and 52.8 %. The binding constants K a for the interaction of S/R -profenofos and 2-Bromo-4-chlorophenol with BSA were 4.70, 4.66 and 4.23 × 10 4 M −1 , respectively. The LC 50 of S/R -profenofos and 2-Bromo-4-chlorophenol for zebrafish were about 0.670, 0.739 and 0.954 mg/L, while the acetylcholinesterase inhibition rate were 86.64 %, 81.21 % and 73.64 %, respectively. The toxicity levels follows the pattern of S -profenofos> R -profenofos>2-Bromo-4-chlorophenol, which was also confirmed by the molecular docking and cell experiments. This study evaluated the interaction mechanism between chiral pesticides and model proteins at the molecular level, providing a new perspective on the food safety risk of chiral pesticides.
研究领域:
环境科学
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分子生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2014
·
IF 1.552
Zhongkui Zhao,Renzhi Li,Yu Li
A clean and highly efficient synthesis of 1-amino-2-acetylanthraquinone via reductive isoxazole ring cleavage of 3-methylanthra[1,2- c ]isoxazole-6,11-dione catalyzed by trace copper using hydrazine hydrate as a clean reducing agent and water as a green reaction medium under mild reaction conditions was investigated. Various transition-metal catalysts were screened for the reductive ring-opening reaction, and Cu(NO 3 ) 2 was shown to be an excellent catalyst. A conversion of 97.2% and 1-amino-2-acetylanthraquinone selectivity greater than 95% were obtained in the presence of 2.6 mol% Cu(NO 3 ) 2 (turnover number 38) with 1.3 equiv. of hydrazine hydrate for 2 h in water. The structure of the product was confirmed by 1 H nuclear magnetic resonance spectroscopy and mass spectrometry ; the main byproduct was hydroxyl-substituted 1-amino-2-acetylanthraquinone. A possible reaction mechanism for the copper-catalyzed ring cleavage of 3-methylanthra[1,2- c ]isoxazole-6,11-dione with hydrazine hydrate was proposed.
研究领域:
有机化学
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环境化学
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DOI
高影响因子
Frontiers in Microbiology
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2025
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IF 4.5
Qiuqian Zeng,Xiaomei Lyu
Introduction: Cellobiose 2-epimerase (CE) catalyzes the interconversion of glucosyl and mannosyl groups at the reducing end of β-1,4-linked disaccharides. This enzyme is pivotal for converting lactose into prebiotics like epilactose, offering a potential solution for lactose-intolerant-friendly dairy products. However, current CEs are hindered by pH and thermal instability in milk processing, as their neutral-to-alkaline pH optima clash with milk’s mildly acidic conditions (pH 6.5–6.7), and their poor thermolability requires costly post-processing enzyme removal.Methods: We identified a novel CE from the acidophilic Acidobacteriota bacterium (Acba-CE) and characterized its properties. Its enzymatic activity was assessed under varying pH and temperature conditions, including milk-processing environments.Results: Acba-CE exhibits an acidic pH optimum (6.0), retaining 95% activity at milk pH (6.5). Notably, it undergoes rapid thermal inactivation at pasteurization temperatures, enabling complete enzyme deactivation during standard pasteurization without additional steps. In milk systems, Acba-CE achieves 28.5% lactose-to-epilactose conversion at refrigeration temperatures (10°C), demonstrating strong cold adaptability.Discussion: To our knowledge, this is the first reported CE from the Acidobacteriota phylum, combining acidophilic activity with low-temperature adaptability. Acba-CE represents a breakthrough for in situ dairy modification, eliminating key bottlenecks in milk processing and enabling next-generation functional milk production.
研究领域:
生物化学
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食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Bokai Ma,Fujian Xu,Mian He,Yuqing Lin,Guanghui Hu,Mei Zhang,Xinying Zhao,Weili Liu
Rhodamine B hydrazide (RBH) was prepared as a new derivatizing reagent for residual formaldehyde in a drug such as N-butyl-2-cyanoacrylate (NBCA). RBH reacted with formaldehyde rapidly at room temperature, and the resulting derivative can be detected sensitively at 238 nm. After optimizing the derivatization and chromatographic separation conditions, RBH as a pre-column derivatizing reagent has been successfully used for the analysis of formaldehyde in real NBCA samples by high-performance liquid chromatography (HPLC). The detection limit of the method is 0.004 μg/mL for formaldehyde, and the recovery is 94.0%-96.6%. Most notably, compared with the previous HPLC methods, the developed method has the advantages of mild derivatization temperature, short derivatization time and high sensitivity.
研究领域:
分析化学
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药物分析
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DOI
高影响因子
AAPS PHARMSCITECH
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2012
·Vol. 13
·期 3
·836-845
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IF 1.432
Han Lina,Zhao Yuefang,Yin Lifang,Li Ruiming,Liang Yang,Huang Huan,Pan Shirong,Wu Chuanbin,Feng Min
In the present study, we developed novel insulin-loaded hyaluronic acid (HA) nanoparticles for insulin delivery. The insulin-loaded HA nanoparticles were prepared by reverse-emulsion-freeze-drying method. This method led to a homogenous population of small HA nanoparticles with average size of 182.2 nm and achieved high insulin entrapment efficiencies (approximately 95%). The pH-sensitive HA nanoparticles as an oral delivery carrier showed advantages in protecting insulin against the strongly acidic environment of the stomach, and not destroying the junction integrity of epithelial cells which promise long-term safety for chronic insulin treatment. The results of transport experiments suggested that insulin-loaded HA nanoparticles were transported across Caco-2 cell monolayers mainly via transcellular pathway and their apparent permeability coefficient from apical to basolateral had more than twofold increase compared with insulin solution. The efflux ratio of P
app (B to A) to P
app (A to B) less than 1 demonstrated that HA nanoparticle-mediated transport of insulin across Caco-2 cell monolayers underwent active transport. The results of permeability through the rat small intestine confirmed that HA nanoparticles significantly enhanced insulin transport through the duodenum and ileum. Diabetic rats treated with oral insulin-loaded HA nanoparticles also showed stronger hypoglycemic effects than insulin solution. Therefore, these HA nanoparticles could be a promising candidate for oral insulin delivery.
研究领域:
药剂学
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生物医学工程
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 6
·1-8
·
IF 5.705
Huang Huayu,Wang Mingxia,Wang Yang,Li Xinli,Niu Zhiying,Wang Xingyu,Song Jinxi
Molybdenum disulfide (MoS 2 ) was used as an electrically conductive skeleton and functionalized with an ionic liquid and gold/silver nanorods. The resulting composite was characterized by scanning electron microscopy, transmission electron microscopy and UV–vis spectroscopy. The composites were used to modify a glassy carbon electrode (GCE) to obtain a sensor for 2,4-dichlorophenol (2,4-DCP). The results show that the oxidation power and electrocatalytic activity of the modified GCE towards 2,4-DCP are enhanced compared to a bare GCE and other modified GCEs. Response is linear in the 0.01 to 50 μM 2,4-DCP concentration range, with a 2.6 nM detection limit. The sensor is highly sensitive and long-term stable. It was successfully applied to the determination of 2,4-DCP in spiked water samples and gave satisfactory recoveries.
Schematic of an electrochemical sensor for the differential pulse voltammetric (DPV) determination of 2,4-dichlorophenol. It is based on the use of an MoS 2 -ionic liquid-Au/Ag nanorod composite.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
Journal of Biobased Materials and Bioenergy
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2016
·Vol. 10
·期 5
·378-384
·
IF 0.635
Chen Wei,Jiang Yetao,Sun Yong,Wang Yanjun,Li Zheng,Wang Xiaoyu,Zeng Xianhai,Liu Shijie,Lin Lu
5-Dimethylaminomethyl-2-Furanmethanol (DMMF) is an essential precursor for the production of ranitidine, a drug for gastropathy. Herein a facile pathway to the value-added pharmaceutical intermediate DMMF from carbohydrates, such as glucose and fructose, was developed for the first
time. The reaction was carried out under catalyst-free conditions using N, N-dimethyl formamide (DMF) as the amine donor in formic acid (FA). A plausible reaction mechanism from carbohydrates to DMMF was also proposed.
研究领域:
有机化学
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药物化学
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生物转化
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DOI
高影响因子
Reaction Chemistry & Engineering
·
2025
·
IF 3.1
Xue Luo,Jiahui Liu,Danhui Li,Lili Zhao,Hualiang An
Cyclopentanone self-condensation to 2-cyclopentylidenecyclopentanone is one of the key steps in synthesizing high-density fuels. In this study, MgAl-LDO(NO3−) catalysts, prepared via coprecipitation, demonstrated good catalytic performance. Meanwhile, Mg2Al-LDO(NO3−) catalysts with different Mg/Al ratios were explored. Characterization by XRD, NH3-TPD and CO2-TPD revealed that Mg2Al-LDO(NO3−) possessed appropriate acid–base active sites, correlating with its enhanced catalytic performance. Under optimized reaction conditions, cyclopentanone conversion reached 85.9% with 98.8% selectivity toward 2-cyclopentylidenecyclopentanone. Furthermore, the reusability of the catalyst was investigated. XRD, SEM, and N2 adsorption–desorption analyses indicated that structural changes in the Mg2Al-LDO(NO3−) catalyst led to its deactivation. GC-MS analysis of the reaction system identified the primary by-product as 2,5-dicyclopentylidenecyclopentanone. Based on these findings, a plausible reaction network is proposed.
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产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Bin Yang,Xiaowei Zeng,Jin Zhang,Jilie Kong,Xueen Fang
Delta (B.1.617.2), a highly infectious variant of SARS-CoV-2, has been sweeping the world, and threatening the safety of human life seriously. It is urgent to develop a highly selective and sensitive assay to accurately identify the SARS-CoV-2 variant Delta. In this work, we constructed a graphene/CRISPR-dCas9 electrochemical biosensor to accurately identify SARS-CoV-2 variant Delta, where the signal was further amplified by embedded electrochemical probe [Ru(phen) 2 dppz]BF 4 . This detection assay could be finished within 47 min totally, with the detection limit of 1.2 pM and good reproducibility with a C·V.% of 2.48% (n = 5). And the biosensor could selectively identify Delta among SARS-CoV-2 and other variants, including Alpha, Beta, Gamma. This assay was further validated by 26 real clinical samples, showing 100% clinical sensitivity and 100% clinical specificity, which provides a new direction for identifying other SARS-CoV-2 variants in the future.
研究领域:
生物技术
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电化学
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分子生物学
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病毒学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 30
·6138-6146
·
IF 4.543
Lijuan Zhang,Junmei Sun,Yunshan Zhou,Yuxu Zhong,Ying Ying,Yanqin Li,Yanqin Liu,Zareen Zuhra,Chunqian Huang
Cu3(BTC)2 (H3BTC = 1,3,5-benzenetricarboxylic acid) was anchored onto the surface of carboxymethylated chitosan non-woven fabrics by a controllable layer-by-layer technique in alternating solution baths of Cu(OAc)2·H2O and H3BTC solutions, and the resulting [Cu3(BTC)2]n@chitosan non-woven fabric composite materials (n = number of alternate deposition cycles) were thoroughly characterized. The results showed that the composite materials not only exhibited excellent decontamination ability against sulfur mustard (HD), with an enhanced degradation rate of HD with increasing n, but also possessed remarkable haemostasis performance. The degradation efficiency of sulfur mustard by [Cu3(BTC)2]4@chitosan was found to be much higher than that of pristine [Cu3(BTC)2]4. The inherent haemostatic capabilities of the chitosan non-woven fabrics were not affected by the growth of Cu3(BTC)2 on the surface of chitosan. Oral and histological toxicity examinations of the pristine Cu3(BTC)2 sample showed that damage and toxicity in the viscera of mice was very low, even if the intra-gastric administration dose of Cu3(BTC)2 reached a very high level (50 mg of Cu3(BTC)2 per kg of mouse). The results have shown that the as-prepared composite can be used safely as a promising haemostatic decontaminant with good recyclability against sulfur mustard.
研究领域:
材料科学
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生物医学工程
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化学
✓ 已验证
DOI
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Weiyou Zhou,Jiayin Huang,Anwei Wang,Jia Cui,Jie Yao,Tao Hou,Shuliang Ji,Mingyang He,Zhonghua Sun
An efficient heterogeneous catalytic system for the [4 + 2] annulation reactions between tertiary anilines and tetrahydrofuran has been developed based on CuCoFe layered double oxide in the study. Cu + species was believed to play the crucial role in the catalytic transformation, and synergistic effect between Cu and CoFe species was observed in the reaction. Various tertiary anilines could be tolerated by the present catalytic system. A series of control experiments were conducted to determine the reaction intermediates. A distinctive reaction mechanism has been described for the annulation reaction based on these results and kinetic isotopic effects experiments.
研究领域:
化学
·
催化科学
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Yue Wang,Wenxiu Li,Tao Zhang
The separation of 2-methylfuran (2-MF) and methanol (MeOH) azeotropes presents a critical challenge in biofuel production due to the efficiency and sustainability limitations of conventional methods. In this study, three ionic liquids (ILs)—1-ethyl-3-methylimidazolium hydrogen sulfate ([EMIM][HSO 4 ]), 1-ethyl-3-methylimidazolium thiocyanate ([EMIM][SCN]), and 1-ethyl-3-methylimidazolium dicyanamide ([EMIM][DCA])—were selected as green extractants for 2-MF – MeOH azeotropic separation. Ternary liquid-liquid equilibrium (LLE) data for the system 2-MF (1) + MeOH (2) + IL (3) were experimentally determined at 303.15 K and 101.325 kPa. The order of both distribution coefficient and selectivity is [EMIM][HSO 4 ] > [EMIM][SCN] > [EMIM][DCA]. Then, the NRTL model was subsequently employed to regress the experimental data, and the accuracy of the NRTL binary parameters was verified through the “GMcal_TieLinesLL” tool. Further mechanistic investigation integrated theoretical approaches (ESP, IGMH, RDF, and MSD analysis) with experimental validation via FT-IR spectroscopy. This combined methodology conclusively elucidates the pivotal role of hydrogen bonding in the extraction and separation process.
研究领域:
化学工程
·
分离技术
·
物理化学
·
绿色化学
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生物燃料
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）