TALANTA
·
2022
·
IF 6.556
Zhimin Liu,Jie Wang,Chen Cui,Limei Zheng,Leqian Hu
An ultrasensitive resonance energy transfer based “on-off” switch electrochemiluminescence (ECL) immunosensing strategy was proposed and applied for prostate specific antigen (PSA) detection. In detail, self-enhancing nanohybrids of nano-CeO 2 and AuNPs decorated graphitic carbon nitride quantum dots (CeO 2 –AuNPs–g-CNQDs) were synthesized, which produced high ECL emission with coreactant K 2 S 2 O 8 for “switch-on” state. Noticeably, due to the reversible Ce 3+ ↔ Ce 4+ reaction, nano-CeO 2 could serve as highly-efficient coreaction accelerator and induce the decomposition of S 2 O 8 2− into SO 4 • - , which endowed the g-CNQDs/K 2 S 2 O 8 system with enhanced ECL emission. Moreover, AuNPs acted crucial role in promoting the electronic transmission rate and providing sites to immobilize the biomolecules. On the other hand, the AgNPs-VB 2 nanocomposites were used as dual labels to quench the ECL signal of g-CNQDs-K 2 S 2 O 8 system via ECL-RET, leading to the decreased ECL for “switch-off” state. On the basis of sandwich-typed immunosensing strategy, the developed ECL immunosensing platform exhibited sensitive response to PSA in a linear range of 1.0 × 10 −5 _ 50 ng mL −1 with a low detection limit of 0.0045 pg mL −1 ( S/N = 3). This work provided a promising tool in early clinical diagnostics of prostate cancer.
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高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Kwabena Agyarko Sarpong,Kun Zhang,Yu Luan,Yunfei Cao,Wanzhen Xu
Tetrabromobisphenol-S (TBBPS) is a Brominated Flame Retardants (BFRs). TBBPS in trace quantities is lethal and do not only alter the normal performance of the endocrine system but can directly or indirectly provoke hepatoxicity and carcinogenic diseases. Herein, we fabricate a facile electrochemical sensor and investigate its application towards the determination of TBBPS through imprinting technique. TBBPS imprinting was carried out in the presence of methacrylic acid (MAA) and 4-aminothiophenol (4-ATP) functional monomers (DiM) over gold nanoparticles (AuNPs) modified carbon paste electrode (CPE). Transmission electron microscopy (TEM) was used to characterize the imprinted sensor. Performance was thoroughly investigated using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The proposed sensor showed detection behavior towards TBBPS in standard solutions with different concentrations range of 0.1 nM-10 nM and with a detection limit of 0.029 nM. The developed MIP(DiM)@AuNPs/CPE sensor had enhanced recognition capacity compared to MIP electrochemical sensors developed with a mono functional monomer (MIP(MoM)@AuNPs/CPE) and non-imprinted sensors. In addition, the low cost; easier preparation; excellent stability; and good selectivity of MIP(DiM)@AuNPs/CPE electrochemical sensor makes it an attractive alternative to high instrumentation detection techniques.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 2
·1-10
·
IF 5.3
Li Hua-Li,Liang Jie-Ling,Li Shun-xing,Liu Feng-jiao,Luo Jia-Yi,Huang Zhao-Jing,Zheng Feng-Ying,Su Ling-Ling,Zhuo You-Bin
The detection of cysteine (Cys) and homocysteine (Hcy) in biological fluids has great significance for early diagnosis, including Alzheimer's and Parkinson's disease. The simultaneous determination of Cys and Hcy with a single probe is still a huge challenge. To enlarge the differences in space structure (line and ring) and energy (-721.78 and -761.08 Hartree) between Cys and Hcy, and to cause a difference of aggregation kinetics, gold nanoparticles (AuNPs) are capped with hydrophilic and low-toxic polyvinylpyrrolidone (PVP) (named AuNPs@PVP) and some surface-active sites of AuNPs are masked, the active area for the binding between AuNPs and the detection object is confined, meanwhile, the stability of AuNPs is improved. A novel nanosensor based on confined active area and aggregation kinetics of AuNPs@PVP, is designed for the identification and determination of Cys and Hcy in 1 and 3 min, respectively, with sufficiently low detection limit (4.12 and 4.35 μM) and linear range (4.12–100 μM) for health evaluation. This single colorimetric sensor was applied successfully to the determination of urine and serum, evidencing high anti-interference ability. Graphical
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qi Zhao,Zhencheng Chen,Chen-Wei Shan,Tao Zhan,Chen-Yang Han,Guo-Cheng Han,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
Human chorionic gonadotropin (HCG), a vital protein for pregnancy determination and a marker for trophoblastic diseases , finds application in monitoring early pregnancy and ectopic pregnancy . This study presents an innovative approach employing electrochemical immunosensors for enhanced HCG detection, utilizing Anti-HCG antibodies and gold nanoparticles (AuNPs) in the sensor platform. Two sensor configurations were optimized: BSA/Anti-HCG/c-AuNPs/MEL/e-AuNPs/SPCE with [Fe(CN) 6 ] 3−/4− as a redox probe (1) and BSA/Anti-HCG/PPy/e-AuNPs/SPCE using polypyrrole (PPy) as a redox probe (2). The first sensor offers linear correlation in the 0.10–500.00 pg∙mL −1 HCG range, with a limit of detection (LOD) of 0.06 pg∙mL −1 , sensitivity of 32.25 μA∙pg −1 ∙mL∙cm −2 , RSD <2.47 %, and a recovery rate of 101.03–104.81 %. The second sensor widens the HCG detection range (40.00 fg∙mL −1 -5.00 pg∙mL −1 ) with a LOD of 16.53 fg∙mL −1 , ensuring precision (RSD <1.04 %) and a recovery range of 94.61–106.07 % in serum samples. These electrochemical immunosensors have transformative potential in biomarker detection, offering enhanced sensitivity, selectivity , and stability for advanced healthcare diagnostics.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Peipei Song,Zhirui Fan,Shuai Sun,Chengye Sun,Jun Wang
In recent years, lead (Pb) contaminated soil has caused irreversible damage to human beings. It is urgent to develop a novel technology for accurate detection of Pb in the soil solution. Herein, a new electrochemical sensor was fabricated. Fe/ZIF-8 was used as the modification material, gold nanoparticles (AuNPs) were electrodeposited, and DNAzyme was applied on the surface as the probe. Under the optimum conditions (DNAzyme concentration of 1 μmol, fixation time of 4 min, incubation time of 17 h and reaction time of 30 min), the detection range was 5×10 −12 ∼ 5×10 −5 M, and the minimum detection limit was 10 −13 M (3δ). Furthermore, the electrochemical sensor demonstrated outstanding anti-interference ability to common cations, anions, organic compounds and other interference factors. After four consecutive tests, the relative standard deviation (RSD) was only 0.39%, and it still displayed excellent reproducibility with 0.69% of RSD. After 20 days, the sensor’s response to Pb 2+ remained at 93.58%. In addition, the sensor could accurately detect Pb 2+ in the actual soil solutions, and the recoveries of the samples ranged from 92% to 109.4%. In conclusion, this electrochemical sensor can specifically detect Pb 2+ in the soil solution with enhanced sensitivity, anti-interference capability and stability, which is of great significance for Pb 2+ detection in the field.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Qinqin Chen,Xing Jin,Qifan Zheng,Min He,Liqiao Han,Jun Yan,Min Zhan,Pengwei Zhang,Peifeng Ke,Jianbing Wang,Xianzhang Huang,Qiaoxuan Zhang
High-affinity anti-double-stranded DNA (anti-dsDNA) antibodies serve as essential biomarkers for systemic lupus erythematosus (SLE), exhibiting particularly strong correlations with active disease manifestations and renal pathology. However, conventional detection methods require urea treatment to quantify these antibodies, necessitated by the heterogeneity of endogenous antibodies, which complicates assay procedures and compromises accuracy. In this study, a novel ratiometric electrochemical (EC) biosensor for the direct detection of high-affinity anti-dsDNA antibodies in serum without additional sample processing has been developed. This biosensor conducted by a novel T(6–4)T dimer dsDNA capture probe and integrated with a self-assembled 4-mercaptophenylboronic acid@gold nanoparticles@6-ferrocenyl-1-hexanethiol (4-MPBA@AuNPs@6-(Fc)HT) composite signal tag. Leveraging the highly specific of the T(6–4)T dimer dsDNA capture probe and multi-amplification capability of 4-MPBA@AuNPs@6-(Fc)HT, the platform exhibited non-specific interferences, higher sensitivity (limit of detection:1.60 IU/mL), and efficient preparation. Clinical serum analysis showed good correlation with indirect immunofluorescence (IIF) (r = 0.629, p < 0.001), superior diagnostic performance (AUC = 0.893 vs. IIF AUC = 0.900) in differentiating SLE patient from healthy control (CON). Notably, EC results correlated more strongly with renal impairment (r = 0.426, p < 0.01) than IIF (r = 0.321, p > 0.05), highlighting its superior clinical utility for monitoring disease. This strategy provides a robust and straightforward approach for serum high-affinity anti-dsDNA antibodies detection, offering significant potential for SLE diagnosis and progression monitoring.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Fengjun Lv,Jinyi Chen,Shengxi Wu,Ran Sun,Lu Zhang,Yawen Luo,Hao Zhang,Doudou Xu
This study developed a stable, label-free AuNPs/Fe-MOFs/COOH-GO electrochemical immunosensor for the detection of Cytokeratin 19 fragment (CYFRA21-1), a critical biomarker for the early screening and diagnosis of non-small cell lung cancer (NSCLC). The nanocomposites of AuNPs/Fe-MOFs/COOH-GO was synthesized using a combination of hydrothermal synthesis, chemical reduction, and ultrasonic dispersion. AuNPs and COOH-GO components contributed high conductivity and a large specific surface area, which significantly enhanced the sensitivity of the sensor. The three-dimensional mesoporous structure of the Fe-MOFs further improved the stability of the immunosensor. Firstly, our novel nanocomposites was modified by using a glassy carbon electrode (GCE). CYFRA21-1 antibodies were then immobilized on the modified surface via interactions with thiol and carboxyl groups present on the nanomaterial. Bovine serum albumin (BSA) was employed to block non-specific binding sites. The constructed immunosensor exhibited a wide detection range for CYFRA21-1 from 1 pg mL −1 to 100,000 pg mL −1 , with a correlation coefficient (R 2 ) of 0.9989 in differential pulse voltammetry, and the limit of detection was defined as 0.35 pg mL −1 . The sensor also demonstrated high sensitivity, excellent specificity, good reproducibility, and remarkable stability, indicating its potent application potential for the early screening and diagnosis of NSCLC.
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Clement Yaw Effah,Longlong Tan,Qimeng Zhang,Xinyu Li,Lijun Miao,Xiaolei Zhou,Lihua Ding,Yongjun Wu
Pseudomonas aeruginosa ( PA ) is an opportunistic pathogen that is widely distributed in soil, water, air, food, animal, and human body surfaces, and is also one of the main pathogens of iatrogenic infections which can cause various infections in people with weakened immune systems. The rapid detection of PA in varied environmental and clinical samples is important for hospital infection control, clinical diagnosis, and food and environmental safety monitoring. In this study, we constructed an efficient separation and enrichment surface-enhanced Raman scattering (SERS) platform based on a dual recognition strategy, and supplemented it with a machine learning nonlinear regression model to achieve rapid, highly sensitive, accurate, and specific detection of PA. By combining the SERS platform with support vector machine learning regression model, a detection limit of 15 CFU/mL with a liner range of 50 to 10 8 cells/mL was achieved. The highly sensitive dual-identification tag-type SERS quantitative analysis system established in this study has the advantages of wide detection range, low detection limit, good specificity, high accuracy and short detection time, thereby providing a method for the prevention and clinical diagnosis of PA infection-related diseases as well as food and environmental safety monitoring.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Ming Sui,Yanni Huang,Yang Tang,Jiaoyang Luo,Yiyang Dong,Yifei Guo,Yonggui Ma,Wei Gu,Mengyue Guo,Jingdan Huang,Dandan Kong,Jinping Li,Meihua Yang
Medicinal teas (MT) were popular in Asian countries for its various effective components. However, Pb pollution poses a serious threat to MT consumption due to the irreversible effect on enzyme activity and neurodegenerative disease. Owing to its toxicity and matrix interference, a rapid screening method for Pb at trace level with high sensitivity and accuracy is urgent for MT consumers. Herein, a simple and ultra-sensitive AuNPs/MWCNTs-MB/DNA-GCE quadruplex biosensor was developed and investigated. Specifically, the monitoring technology of scanning electrochemical microscope was utilized to analyze the assembly and application process for screening key parameters in complex matrix , through in-situ characterization of the microstructure changes on stepwise modified sensors. According to the combined results of electrochemical test and Randles-Sevcik equation, the proposed sensor showed a wide linear range from 5.0 * 10 −15 to 5.0 * 10 −13 M with LOD of 0.9 fM. In addition, the optimized sensor exhibited an excellent selectivity under the inference of metals and medicinal components. The proposed biosensor was successfully implemented for the ultrasensitive monitoring Pb pollution in MT while its water infusions recoveries maintained within acceptable range (81–118 %, 82–129 %). In all, AuNPs/MWCNTs-MB/DNA-GCE provided a new insight into the design, optimization, application and mechanism analysis of sensor for reliable sensing in controlling MT pollution and consumption safety.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaodong Cao,Minli Liu,Jie Lu,Hongge Lv,Jinzhe Han,Shudong He,Yongkang Ye,Xiangyang Chen,Zhaojun Wei,Haisong Zheng
This work presented an electrochemical biosensor for the detection of virulence outer membrane protein A ( ompA ) gene of Cronobacter sakazakii ( C. sakazakii ), which was based on the mimic peroxidase activity of boron doped quantum dots-Au nanoparticles (BQDs-AuNPs) and a signal amplification strategy of exonuclease III (Exo III)-assisted target-recycling (EATR). The electrochemical signal was come from the electrochemical reduction of H 2 O 2 by BQDs-AuNPs nanozyme. Due to the enhanced peroxidase-mimic electrocatalytic efficiency of BQDs-AuNPs and the EATR strategy, the biosensor showed a broad linear range (1.0 × 10 -15 – 1.0 × 10 -8 mol L -1 ) and a low limit of detection (LOD, 4.0 × 10 -17 mol L -1 ). The constructed biosensor could also be applied in direct detection of extracted DNA from C. sakazakii . A good linear relationship (7.8 – 7.8 × 10 6 CFU mL −1 ) between the logarithm concentration of C. sakazakii and electrochemical signal was obtained with a LOD of 2.6 CFU mL −1 . The biosensor was applied in the detection of impA gene segments in contaminated infant formula with recoveries ranged in 83.4 – 108.2%.
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ANALYST
·
2018
·Vol. 143
·期 21
·5210-5217
·
IF 3.864
Jinxin Chi,Maolin Chen,Lijun Deng,Xucong Lin,Zenghong Xie
A convenient and high-performance AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolithic column with an unusually high coverage density of aptamers was facilely prepared and used for on-column selective recognition of ochratoxin A (OTA). Due to the high surface-to-volume ratio of AuNPs, the robust conjugation of Au-SH and large specific surface area of hybrid-silica monolith, high coverage density of 5′-SH-aptamers up to 3494 pmol μL−1 was achieved, which was 2.5–10 folds higher than that of other previously reported affinity monoliths modified with AuNPs@Apt. Using OTA as the model analyte, the highly selective recognition of OTA was carried out via online coupling with HPLC, and the cross-reactivity towards analogues, such as OTB and aflatoxin B1, was weak. High recovery yields of OTA were achieved at more than 92% (n = 3) even when OTB was added at a high concentration level up to 50 ng mL−1. For sample analysis, efficient discrimination of OTA was successfully obtained with a sensitive detection limit of 25 pg mL−1. The recoveries of OTA with different fortified levels were achieved at 88.6%–94.1% and 88.2%–94.3% for beer and wine samples, respectively. This protocol provides a facile approach for fabricating a desirable affinity monolith modified with abundant aptamers for highly selective and sensitive on-column extraction of target analyte OTA.
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BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Zhiyong Tang,Junlin He,Jun Chen,Yazhen Niu,Yilin Zhao,Yuchan Zhang,Chao Yu
We are presenting an electrochemical immunosensor for the determination of Galectin-3 (Gal-3), a biomarker of heart failure. A glassy carbon electrode (GCE) was modified with a film of a composite made from the N-doped graphene nanoribbons immobilized Fe-based-Metal-organic frameworks deposited with Au nanoparticles (N-GNRs-Fe-MOFs@AuNPs). Primary antibody against Gal-3 (Gal-3-Ab 1 ) was immobilized on the Au nanoparticles on the surface of the modified GCE which then was blocked with bovine serum albumin . Signal amplification is crucial for obtaining low detection limits in biosensors. Here, a relatively simple and ultrasensitive sandwich-type electrochemical immunosensor based on novel signal generation and amplification was developed for the determination of Gal-3. A kind of novel redox-active species, AuPt-Methylene blue (MB) (AuPt-MB) nanocomposites , was synthesized by a one-pot method for the first time. Wherein, MB as a kind of the electron transfer mediators in an amperometric biosensor is responsible for electron production and signal amplification. The rod-like AuPt-MB nanohybrids displayed uniform morphology and good electrochemical activity and can combine with the second antibodies against Gal-3 (Gal-3-Ab 2 ). And the AuPt-MB-Ab 2 coupled with the N-GNRs-Fe-MOFs@AuNPs-Ab 1 to form the sandwich type format that can greatly enhance the biosensor's sensitivity. Under optimal conditions, the designed immunosensor exhibited a linear concentration range from 100 fg mL −1 to 50 ng mL −1 , with a low detection limit of 33.33 fg mL −1 (S/N = 3) for Gal-3 in spiked serum. Additionally, the designed immunosensor showed acceptable selectivity, reproducibility and stability. The satisfactory results in analyzing human serum samples indicated potential application promising in monitoring biomarkers.
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