RSC Advances
·
2022
·Vol. 12
·期 51
·33284-33292
·
IF 4.036
Lixing Kang,Yan Zhang,Qian Gong,Chandreyee Manas Das,Huilin Shao,Daniel Puiu Poenar,Philippe Coquet,Ken-Tye Yong
This paper reports the fabrication, testing and obtained performance of a plasmonic sensor employing a gold (Au) nanohole array chip coated with tungsten disulphide (WS2), which is then functionalized for the detection of protein–protein interactions. A key novelty is that the WS2 was deposited as a monoatomic layer using a wafer-scale synthesis method that successfully provided a film of both high quality and uniform thickness. The deposited WS2 film was transferred onto a Au nanohole array chip using a novel method and was subsequently functionalized with biotin. The final sensor was tested and it demonstrated efficient real-time and label-free plasmonic detection of biotin–streptavidin coupling. Specifically, compared to a standard (i.e. uncoated) Au nanohole-based sensor, our WS2-coated Au nanohole array boosted the spectral shift of the resonance wavelength by ∼190%, resulting in a 7.64-fold improvement of the limit of detection (LOD).
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Road Materials and Pavement Design
·
2019
·
IF 1.98
Xuejuan Cao,Mei Deng,Boming Tang,Yongjie Ding,Xiaoyu Yang
Nitric oxide (NO) emitted from vehicle exhausts are associated with adverse health effects on the public. This study prepared a graphitic carbon nitride (g-C3N4)/TiO2 composite using melamine and titanium dioxide (TiO2) and then applied the composite on chip seal for the photocatalytic removal of NO. The g-C3N4/TiO2 composite was synthesised by an ultrasonic-assisted solid-phase synthetic method. The results of X-ray powder diffraction, Fourier-transform infrared spectroscopy, and a scanning electron microscope indicated that g-C3N4 and TiO2 were successfully synthesised and most of the sheet structures of g-C3N4 were attached by approximately spherical TiO2 particles. The UV–Vis and PL characterisation suggested that optical absorption range of TiO2 was broadened and the separation of electronic-holes was hindered, which contributed significantly to the enhancement of the visible-light photocatalytic activity. The loading capacity of 0.0092 kg/m2 g-C3N4/TiO2 composite on chip seal specimens had the highest NO removal efficiency of 31.41% within 30 min.
研究领域:
环境科学
·
材料科学
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TALANTA
·
2026
·
IF 6.1
Lihua Mou,Jianzhou Feng,Mingli Ye,Zhengqu Guo,Xiaoya Hu,Lingling Wu,Yun Shu
The efficient isolation and detection of tumor-derived extracellular vesicles (T-EVs) are vital for the early diagnosis of cancer. Herein, a magnetic reversible aptamer microfluidic chip (MRA-Chip) combined with a MOF-based electrochemical sensor was developed for the efficient isolation and detection of T-EVs. First, the aptamer-functionalized magnetic beads (MBs) were fixed in the herringbone chip to construct a magnetic reversible affinity interface, which improves the capture efficiency of T-EVs by 115 % compared with traditional affinity interfaces due to the enhanced interface collision induced by the vortex mixing effect of the herringbone structure and the high density of MBs distributed on the capture interface. Subsequently, magnet withdrawing induced T-EVs release with high efficiency. The Zr metal-organic framework (MOF) loaded with methylene blue binds to the EVs through the interaction of the Zr–O–P bond. Then, the EVs were quantitatively analyzed based on the electrochemical signal of Zr MOF@methylene blue, and the limit of detection is as low as 6.5 particles/μL. At last, this method has been successfully applied to the detection of clinical samples and can effectively distinguish between cancer patients and healthy individuals with 100 % accuracy. This method opens a new avenue for isolation and detection of T-EVs, and has great application prospects in the early diagnosis and treatment of cancer.
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Soil & Environmental Health
·
2026
·
IF 9.4
Yilun Lan,Xiangyu Zhu,Wentao Yu,Kun Wang,Baohai Li,Baoliang Chen
The rhizosphere is microscopically heterogeneous, yet the impact of uneven distribution of nanomaterials (NMs) on roots remains unclear. Here, using Arabidopsis thaliana and MoS 2 nanosheets as the model plant and NMs, respectively, we employed a microfluidic root chip housed in a Petri dish with moist dust-free paper to investigate root responses to spatially asymmetric exposure. We compared symmetric exposure (half dose delivered to each root side; a homogeneous scenario) with asymmetric exposure (full dose to one side; a heterogeneous scenario) of MoS 2 nanosheets over a 5-day period. Results showed that this spatial distinction alone is sufficient to elicit divergent growth and physiological responses in seedling roots. Real-time imaging revealed that symmetric exposure induced a classic dose-dependent toxicity from excess MoS 2 nanosheets-induced stress oxidative damage, whereas asymmetric exposure elicited a hormesis effect on root growth, with asymmetric exposure of 50 mg L -1 MoS 2 nanosheets for 64 h increasing root length by 18% compared to symmetric exposure at 25 mg L -1 . Mechanistic investigations, integrating dynamic monitoring and inhibition experiments using fluorescence probes and an auxin reporter, showed that localized stimulation promoted root growth via Fe 2+ -dependent reactive oxygen species signaling with asymmetric exposure increasing Fe 2+ and ROS levels by approximately 1.74-fold and 2.03-fold, respectively, relative to symmetric exposure, leading to auxin accumulation in root tips. Our findings demonstrate that spatial heterogeneity is an essential consideration for evaluating and designing the application of redox-active NMs.
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Transboundary and Emerging Diseases
·
2026
·
IF 2.6
Wenju Ren,Rui Yang,Chenyang Qi,Jie Zhou,Liu Yang,Yuandi Yu,Taixiong Zheng,Lizhi Fu
The lack of simple, rapid, accurate, and low‐cost on‐site diagnostic technology has seriously hindered the effective prevention and control of major infectious diseases such as African swine fever (ASF). This study presents an integrated, portable diagnostic system comprising a low‐cost microfluidic chip and a fully automatic, portable device for simultaneously detecting nine samples. The aim is to achieve rapid on‐site detection of African swine fever virus (ASFV) nucleic acid. The system integrates magnetic bead nucleic acid extraction, transfer, loop‐mediated isothermal amplification (LAMP), and optical detection functions, and can perform fully automatic, closed‐tube analysis of 9 samples in 25 min, effectively avoiding aerosol pollution. The detection limit for the ASFV P10 gene is 10 2 copies/μL. In a blind test involving 87 clinical samples, the system demonstrated excellent performance with a clinical sensitivity and specificity of 97.6% (42/44) and 98.0% (42/43), respectively, and an accuracy of 96.6% (84/87). When the sample concentration exceeds the detection limit, sensitivity can reach 100 %. The core advantages of this method are that it is fast, accurate, and low cost, and does not require professional laboratories or operators. Its modular design makes it easy to expand to a 100‐channel, high‐throughput detection system at low cost. This system provides a powerful technical tool for large‐scale epidemic screening and prevention in areas with limited resources, offering significant public health and economic value.
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COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2026
·
IF 8.2
Wen Si,BingYang Ma,LinLin Hou,Shutong Tian,Guanglan Liao,Li Fang,Guoqing Xin,Hu Long,Tielin Shi
Shrinking device dimensions and escalating heat fluxes bottleneck chip thermal management. Boron nitride nanotubes (BNNTs) combine high thermal conductivity with a wide band gap, making them attractive fillers for composites. However, deployment of BNNTs remains constrained by limited production throughput and associated cost barriers. Here, an ammonia-activated high-pressure laser ablation strategy with non-consumable Cu mesh assistance (A-HPLA-CuM) is developed for BNNT synthesis, yielding BNNT-rich fibrous product at 692.7 mg h −1 using a scalable setup. The obtained BNNTs exhibit high crystallinity, with a mean diameter of 9 nm and a mean wall number of 7. Compared with conventional laser ablation and Cu mesh-assisted synthesis in N 2 , ammonia activation is associated with a higher production rate while maintaining product quality. The improvement is likely associated with NH 3 -derived B-N–H intermediates that may facilitate B-N conversion, together with Cu mesh assistance that may help maintain a favorable local reaction environment. As a proof-of-concept validation, the as-synthesized BNNTs were evaluated in composite films. At 50 wt% BNNT loading, the film delivers an in-plane thermal conductivity of 15.9 W m −1 K −1 and reduces temperature by 4.67 ℃ relative to the BN-filled control after 30 s. This work demonstrates A-HPLA-CuM as a scalable route for BNNT synthesis.
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Chemosensors
·
2025
·Vol. 13
·期 4
·138
·
IF 3.7
Yuqi Liu,Nan Qiu,Zhihao Zhang,Yang Li,Chao Bian
In this study, an in situ electrochemical modulation method based on an ultramicro interdigitated array electrode (UIAE) sensor chip was developed for the detection of ammonia nitrogen (NH3-N) in neutral aqueous solutions. One comb of the UIAE was used as the working electrode for both the modulating and sensing functions, while the other comb was used as the counter electrode. Utilizing its enhanced mass transfer and proximity effects, the feasibility of in situ modulation of the solution environment near the UIAE chip to generate an electrochemical response for NH3-N was investigated using electrochemical methods. The proposed method enhances the concentration of hydroxide ions and active chloride in the local solution near the sensor chip. These reactive species play a key role in improving the sensor’s electrocatalytic oxidation capability toward ammonia nitrogen, facilitating the sensitive detection of ammonia nitrogen in neutral environments. A linear relationship was displayed, ranging from 0.15–2.0 mg/L (as nitrogen) with a sensitivity of 3.7936 µA·L·mg−1(0.0664 µA µM−1mm−2), which was 2.45 times that in strong alkaline conditions without modulation. Additionally, the relative standard deviation of the measurement remained below 2.9% over five days of repeated experiments, indicating excellent stability.
研究领域:
环境科学
·
分析化学
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Rubing Zou,Qianyi Yu,Zexiang Wang,Peiyu Yang,Ying Zhao,Yihua Liu,Yan Fu,Yirong Guo
Unexpected interferences from the food matrix pose challenges to achieving high-performance on-site monitoring of pesticide residues. In this study, we employed 3D printing technology to fabricate a miniaturized lab-on-a-chip (LOC) device for in-situ sample pretreatment. Serving as the analytical component, a lateral flow immunochromatographic strip was developed for thiamethoxam, a representative neonicotinoid insecticide. Results showed that the composite LOC device enabled on-site determination of thiamethoxam in 13 different food samples using a multi-step analysis approach. Similar to the buffer-based calibration curve (IC 50 : 0.11 ng·mL −1 ), the matrix-based calibration curves for celery (IC 50 : 0.15 ng·mL −1 ) and cucumber (IC 50 :0.10 ng·mL −1 ) samples exhibited ultrahigh sensitivities, with a total analysis time within 15 min. The average recovery rates were 87.2 % ~ 107.4 %, with relative standard deviations of 2.1 % ~ 7.6 % at the spiked levels of 1 and 5 ng·g −1 . Furthermore, this LOC device was applied to unknown food samples, achieving excellent accordance with LC-MS/MS confirmation.
研究领域:
分析化学
·
食品安全
·
环境科学
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BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Jingwei Chen,Tingting Liu,Yule Zhang,Mengnan Duan,Zhijin Yang,Mengya Chen,Yiran Wang,Lulu Zheng,Songlin Zhuang,Dawei Zhang
Home testing technology strategy is critical for early screening of disease. However, current home testing technologies often require complex processes, which limits their application. In this study, a time-resolved cascade logic gate microfluidic chip (TCLMC) was revealed to enable capillary force-based one-step operation without manual intervention or professional equipment. By analogy with logic gates in the circuit, TCLMC could automatically control the fluid flow and regulate the incubation time to optimize the immunoassay . The limit of detection of TCLMC for SARS-CoV-2 and influenza B virus (Flu B) was 134.94 and 79.17 pg mL −1 within 10 min. Additionally, this study tested saliva samples from 12 Flu B patients and 24 healthy controls to verify its clinical application. The results showed that TCLMC had high sensitivity (100%), specificity (100%), and accuracy (100%). This study provides a new one-step strategy for home testing and demonstrates its great potential in the diagnosis field.
研究领域:
医学诊断
·
生物技术
·
微流体技术
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LANGMUIR
·
2024
·
IF 3.7
Peng Dai,Wenyun Su,Zhaokun Xian,Xiangfu Wei,Shengchang Tang,Guangyong Huang,Cuimin Sun,Wei Han,Ling Zhu,Hui You
Developing a rapid fabrication method for crack-free opal films is a significant challenge with broad applications. We developed a microfluidic platform known as the “filter paper-enhanced evaporation microfluidic chip” (FPEE-chip) for the fabrication of photonic crystal and inverse opal hydrogel (IOPH) films. The chip featured a thin channel formed by bonding double-sided adhesive poly(ethylene terephthalate) with a polymethyl methacrylate cover and a glass substrate. This channel was then filled with nanosphere colloids. The water was guided to evaporate rapidly at the surface of the filter paper, allowing the nanospheres to self-assemble and accumulate within the channel under capillary forces. Experimental results confirmed that the self-assembly method based on the FPEE-chip was a rapid platform for producing high-quality opal, with centimeter-sized opal films achievable in less than an hour. Furthermore, the filter paper altered the stress release mechanism of the opal films during drying, resulting in fewer cracks. This platform was proven capable of producing large-grain, crack-free opal films of up to 30 mm2 in size. We also fabricated crack-free IOPH pH sensors that exhibited color and size responsiveness to pH changes. The coefficient of variation of the gray color distribution for crack-free IOPH ranged from 0.03 to 0.07, which was lower than that of cracked IOPH (ranging from 0.07 to 0.14). Additionally, the grayscale peak value in 1 mm2 of the crack-free IOPH was more than twice that of the cracked IOPH at the same pH. The FPEE-chip demonstrated potential as a candidate for developing vision sensors.
研究领域:
材料科学
·
纳米技术
·
传感器
·
微流控
·
光子学
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TALANTA
·
2025
·
IF 6.1
Haonan Wang,Qingling Nie,Luyao Lin,Ruiyun You,Ziting Ye,Jin Zhang,Yudong Lu,Zhenwen Lin,Zixing Yan
Recent years have witnessed remarkable progress in cellulose and hydrogels, facilitating the development of wearable green sensing technology. This study presents a novel flexible hydrogel patch designed as a skin-adhesive platform for wearable sensors, synthesized through the strategic integration of hydrogel and porous “sponge-like” technologies. Fabricated from acrylamide and sodium alginate matrices enhanced with tannic acid modification, the composite exhibits superior mechanical performance, biocompatibility (98.75 % cell viability), antimicrobial efficacy (92.03 % inhibition rate against S. aureus ), and tissue adhesion. A sulfonated cellulose nanofibril/sodium alginate/silver nanoparticle (S–CNF/SA/Ag NPs) composite membrane was engineered as a surface-enhanced Raman spectroscopy (SERS) detection module, achieving remarkable sensitivity for sweat biomarkers with detection limits of 10 −6 M for urea and 10 −5 M for lactic acid. Experimental validation confirms exceptional epidermal conformability and sustained adhesion. This technological advancement establishes a foundation for transformative applications in personalized healthcare monitoring, exercise physiology assessment, clinical diagnostics, and preventive medicine, thereby accelerating innovation in wearable biosensing platforms.
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ACS Sensors
·
2025
·
IF 9.1
Yingjin Ma,Man-Chung Wong,Menglin Song,Pui Wang,Yuan Liu,Yifei Zhao,Honglin Chen,Juewen Liu,Jianhua Hao
Outbreaks of SARS-CoV-2, first investigated as an unknown pathogen, have reflected the severe threat that pathogen X poses to public health and social security. Early and precise diagnosis and classification of infectious respiratory diseases with similar symptoms are essential for the risk assessment of public health or epidemiological investigations. Current technologies are limited to detect known viruses, leading to false negatives for novel or mutated pathogens. Here, we propose an ML-assisted SERS strategy for screening various types of respiratory viruses and potential pathogen X in cases with similar infectious symptoms. A label-free 3D plasmonic Au-PS SERS chip was designed to amplify the Raman signal over 103-fold compared to a conventional Au substrate. An ensemble ML model was developed to analyze SERS data for effectively distinguishing between healthy individuals, SARS-CoV-2, RSV, and influenza A and B, as well as identifying newly emerging pathogens. Our experiments demonstrated that the ensemble model integrated with SERS spectra achieved a remarkable classification accuracy of 100%. Notably, the model exhibited excellent performance in detecting mixed viral infections and simulated pathogen X, with a reliable detection range of viral concentrations from 5 × 102 to 106 PFU/mL under acoustic enrichment. This approach holds significant promise for the early screening and detection of emerging and known respiratory pathogens.
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