Biomedical Materials
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2022
·Vol. 18
·期 1
·015006
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IF 4.103
Peng Ding,Qianqian Wei,Ning Tian,Xiaoyue Ding,Ling Wang,Bin Wang,Oseweuba Valentine Okoro,Amin Shavandi,Lei Nie
The enzymatically crosslinked hydrogel could replicate the cellular microenvironment for biomedical applications. In the present study, to improve the cytocompatibility of chitosan (CS), sialic acid (SA) was introduced to CS to synthesize sialylated CS (CS-SA), and the tyramine (TA) was grafted to gelatin (G) to obtain TA modified gelatin (G-TA). The successful synthesis of CS-SA and G-TA was confirmed using 1H NMR and UV–Vis absorption spectra. The interpenetrating polymer networks G-TA/CS-SA (GC) hydrogel was then fabricated via blending G-TA and CS-SA solutions and crosslinked using horseradish peroxidase. The storage modulus (G') of the fabricated GC hydrogels with different ratios of G-TA/CS-SA greatly varied during the formation and strain of hydrogels. With the increase of CS-SA concentration from 0% to 2%, the storage modulus of GC hydrogels was also observed to decrease from 1500 Pa to 101 Pa; the water uptake capacity of GC hydrogels increased from 1000% to 4500%. Additionally, the cell counting kit-8 and fluorescent images demonstrated the excellent cytocompatibility of GC hydrogels after culturing with NIH 3T3 cells. The obtained results indicated that the fabricated GC hydrogels might have potential in biomedical fields, such as wound dressing.
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Progress in Natural Science-Materials International
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2022
·
IF 4.269
Ali A. Altam,Liping Zhu,Dafaalla Babiker,Hajo Yagoub,Shuguang Yang
The complex beads of carboxymethyl cellulose (CMC) and chitosan (CHI) that are fabricated by interfacial complexation have a core-shell structure composed of a thin skin layer and a 3D entangled internal structure, which endows the beads with adsorption possibilities toward anionic and cationic molecules via electrostatic interaction. However, the loading and releasing behavior of such a system is essential and fundamental for their application yet has not been studied systematically. In this work, the influences of beads physical state, adsorbent dosage, agitation speed, solution pH and temperature on the loading behavior were investigated. The CMC@CHI beads demonstrated different loading efficiency to three typical dyes of methylene blue (MB), acid blue-113 (AB), and methyl orange (MO) with different charge status. The adsorption rate followed an order of MB ˃ AB ˃ MO, giving 97.4%, 73.3% and 20.2%, respectively. The releasing of three dyes from CMC@CHI beads also exhibited different rates, and MB release was found to be prolonged compared to AB and MO. The loading mechanism of dyes was further investigated via X-ray photoelectron spectroscopy. These CMC@CHI beads may have possible applications in mixed dye removal as well as encapsulating systems of drugs or sensitive chemicals with different charges.
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Biomaterials Advances
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2022
·
IF 0
Shiqing Ma,Xuemei Lu,Xinying Yu,Yaqi Du,Shendan Xu,Minting Li,Cheng Peng,Zihao Liu,Jiayin Deng
Bacteria are recognized as the driving factors of periodontitis. However, excessive reactive oxygen species (ROS) can harm periodontal tissue while also causing an uncontrolled inflammatory response. Hence, eliminating excessive ROS and blocking ROS-induced abnormal inflammatory response by antioxidants are achieving remarkable results in periodontitis therapy. Moreover, influenced by the deep and irregular periodontal pockets, injectable thermo-sensitive chitosan-based hydrogels have attracted a lot of attention. This study aimed to formulate an antibacterial and antioxidant therapeutic regimen by incorporating antimicrobial peptides (Nal-P-113) and/or antioxidants (polydopamine nanoparticles, PDNPs) into chitosan-based hydrogels. The hydrogel was characterized in vitro and finally examined in rats using the experimental periodontitis model. The release kinetics showed that the hydrogel could stably release Nal-P-113 and PDNPs for up to 13 days. The scavenging activity of the hydrogel against DPPH was about 80 % and the antibacterial ratio against Streptococcus gordonii ( S. gordonii ), Fusobacterium nucleatum ( F. nucleatum ) and Porphyromonas gingivalis ( P. gingivalis ) was about 99 %. Importantly, it was examined that the hydrogel had the ability to prevent periodontal tissue damage. Thus, chitosan-based hydrogels may provide a basis for designing multifunctional local drug delivery biomaterials for the treatment of periodontitis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Yanyan Zhang,Gareth R. Williams,Jiadong Lou,Wanting Li,Cuiwei Bai,Tong Wang,Shiwei Niu,Chun Feng,Li-Min Zhu
Targeting the delivery of anti-cancer drugs to a tumor site is essential for effective treatment and to ensure minimal damage to healthy cells and tissues. In this work, a chitosan-based nanoplatform was constructed for combined photothermal therapy and chemotherapy of breast cancer. The pH-sensitive and biocompatible biopolymer chitosan (CS) was grafted with N -vinylcaprolactam (NVCL) and modified with biotin (Bio), imparting it with temperature sensitive property and also the ability for active targeting. The polymer self-assembled to give nanoparticles (NPs) loaded with indocyanine green (ICG) and doxorubicin (DOX). When the NPs are exposed to near-infrared (NIR) laser irradiation, ICG converts the light to heat, inducing a significant phase transition in the NPs and facilitating the release of the drug cargo. In addition, the solubility of chitosan is increased in the slightly acidic microenvironment of the tumor site, which also promotes drug release. A detailed analysis of the NPs both in vitro and in vivo showed that the carrier system is biocompatible, while the drug-loaded NPs are selectively taken up by cancer cells. Particularly when augmented with NIR irradiation, this leads to potent cell death in vitro and also in an in vivo murine xenograft model of breast cancer.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2022
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IF 5.062
Hui Yun Zhou,Jia Nan Tong,Li Jun Ren,Pei Yan Hao,Hui Jie Zheng,Xu Ming Guo,Ya Wei Chen,Jun Bo Li,Hyun Jin Park
Chitosan hydrogel had been widely used in cell culture and tissue engineering, but its long gel time, weak mechanical strength, and weak antibacterial performance limited its application. In this study, the cyclodextrin grafted glutamic acid , a water-soluble cyclodextrin derivatives , was prepared by glutamic acid (Glu) and mono-(6-OTs)-β-CD under alkaline conditions and confirmed by FTIR and 1 H NMR. Then chitosan (CS) and cyclodextrin grafted glutamic acid (CD-g-Glu) were used to prepare chitosan/cyclodextrin derivatives thermosensitive hydrogel (CS/CD-g-Glu) to improve the properties of chitosan-based thermosensitive hydrogels. The CS/CD-g-Glu hydrogel had temperature sensitivity and stable three-dimensional network structure. In addition, the hydrogel loaded with berberine hydrochloride (BBH) showed optimal sustained-release effect, and the cumulative release rate was 57% in 24 h. More importantly, the CS/CD-g-Glu hydrogel loaded with BBH showed prefect antibacterial activity against E. coli and S. aureus . Therefore, the CS/CD-g-Glu hydrogel has great promising applications in drug delivery, tissue engineering, biotechnological, and biomedical fields.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Rong-Rong Yan,Dai Xue,Chang Su,Yan Xu,Jin-Song Gong,Yan-Ling Liu,Min Jiang,Yan Geng,Guo-Zhong Lv,Zheng-Hong Xu,Jin-Song Shi
Uncontrolled bleeding leads to a higher fatality rate in the situation of surgery, traffic accidents and warfare. Traditional hemostatic materials such as bandages are not ideal for uncontrolled or incompressible bleeding. Therefore, it is of great significance to develop a new medical biomaterial with excellent rapid hemostatic effect. Keratin is a natural, biocompatible and biodegradable protein which contains amino acid sequences that induce cell adhesion . As a potential biomedical material , keratin has been developed and paid attention in tissue engineering fields such as promoting wound healing and nerve repair . Herein, a keratin/chitosan (K/C) sponge was prepared to achieve rapid hemostasis . The characterizations of K/C sponge were investigated, including SEM, TGA , liquid absorption and porosity, showing that the high porosity up to 90.12 ± 2.17 % resulted in an excellent blood absorption. The cytotoxicity test and implantation experiment proved that the K/C sponge was biocompatible and biodegradable. Moreover, the prepared K/C sponge showed better hemostatic performance than chitosan sponge (CS) and the commercially available gelatin sponge in both rat tail amputation and liver trauma bleeding models. Further experiments showed that K/C sponge plays a hemostatic role through the endogenous coagulation pathway, thus shortening the activated partial thromboplastin time (APTT) effectively. Therefore, this study provided a K/C sponge which can be served as a promising biomedical hemostatic material.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Ling Ding,Zhao Liu,Shen Song,Mohamed Aamer Abubaker,Lele Chen,Jipeng Shi,Zengjie Fan,Ji Zhang
Hydroxypropyl trimethyl ammonium chloride chitosan (HACC) was synthesized by reacting chitosan with glycidyl trimethylammonium chloride. Atomic force microscopy showed that HACC exhibited disorderly coils in dilute solution and formed a three-dimensional network. Flow, thixotropy , and dynamic viscoelasticity tests were conducted using an MCR301 rheometer. The HACC solution was a non-Newtonian pseudoplastic fluid, and the shear behavior of different concentrations (2–6 %, w/v) was evaluated by the Williamson model fitting. Furthermore, the thixotropy was highly dependent on concentration changes: the high-concentration solution structure was difficult to recover in a short time. The dynamic viscoelasticity test indicated that the viscoelasticity of the HACC solution not only exhibited a viscous behavior similar to that of a fluid, but also exhibited elastic properties of weak gel. HACC exhibited high-strength solid-like gel characteristics at high temperature.
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CARBOHYDRATE POLYMERS
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2022
·
IF 10.723
Yamei Zhang,Shujuan Yang,Haiyun Tang,Sihui Wan,Wenqi Qin,Qu Zeng,Junhao Huang,Gaobo Yu,Yuhong Feng,Jiacheng Li
The regulation of the magnitude of the depletion effect is necessary for accurately predicting and explaining the emulsion stabilization mechanism. Herein, the bacterial cellulose/carboxymethyl chitosan (BC/CCS) complexes with tunable assembled behaviors were prepared and designed via electrostatic interaction . Specially, the emulsions stabilized by BC/CCS complexes exhibited excellent stability as compared with that stabilized by BC polymers alone. At pH 9.6, BC/CCS complexes in the continuous phase induced long-range depletion-stabilization effect to stabilize emulsions. Additionally, the magnitude of depletion effect of BC/CCS complexes could be improved by increasing BC concentration, and effectively stabilized emulsions. Furthermore, with the decrease to pH 7.0, the interfacial adsorption layers at the oil-water interface prevented oil droplets from agglomerating, but did not show better emulsion stability. These results clarified that the magnitude of the depletion effect could be controlled by altering BC-based complexes particles, which would be useful for the applications of emulsions in numerous fields.
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Biomolecules
·
2022
·Vol. 12
·期 8
·1089
·
IF 6.064
Liying Zhang,Gao He,Yang Yu,Yu Zhang,Xiang Li,Shige Wang
In recent years, multifunctional hydrogels have received a great deal of attention because they are biocompatible and can mimic the extracellular matrix. Herein, we prepared hydrogels of biocompatible cross-linked networks with photothermal properties. In this study, a chitosan/polyaniline/laponite (COL) hydrogel with photothermal conversion capability was designed. Polyaniline was firstly grafted onto chitosan and its solution was mixed with oxidized dextran, which was then cross-linked into a hydrogel via a Schiff base reaction. Furthermore, an aluminosilicate clay material, laponite (LAP), was incorporated into the hydrogel. The swelling ratio of the COL hydrogel in various solutions was greater than 580%, and it showed good degradation ability (the mass–loss ratio was over 45% after 28 days). This composite hydrogel was demonstrated to have good photothermal conversion properties and biocompatibility at both the cell (cell viability was over 97%) and animal levels. The COL hydrogel showed a photothermal conversion efficiency of 23.7% under the irradiation of a near-infrared laser. Coupled with the osteogenic differentiation-inducing potential of LAP, the COL hydrogel has the potential to kill tumors via hyperthermia or serve as scaffolds for bone tissue regeneration.Keywords:hydrogel;chitosan;polyaniline;photothermal;biocompatibility
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Wen-Duo Wang,Chao Li,Chun Chen,Xiong Fu,Rui Hai Liu
There is fast increasing interest in the development of alimentary protein stabilized emulsions due to their potential applications in functional food fields. This work studied the effect of glycation degree with chitosan oligosaccharide (COS) on the emulsifying properties of lactoferrin (LF) through Maillard reaction. In the present study, SDS-PAGE and FT-IR were used to confirm LF and COS covalently binding together successfully. Intrinsic fluorescence showed that glycation with COS led more hydrophobic groups exposed to the surface of the structure and particle size increase of LF. Emulsions with 50% ( v /v) oil phase and protein concentration of 2% ( w / v ) was fabricated through one-step shear method. Compared with native LF, emulsions stabilized by LF-COS conjugates showed smaller droplet size and lower creaming index (CI). Among these samples, LF-COS conjugates under 4 h had the best emulsifying efficiency and stability, the emulsion droplet size and the CI of which decreased 39.66% and 28.55% compared with LF, respectively. Furthermore, glycation with COS enhanced the interfacial activity of LF leading to more adsorbing amount and forming thicker layer on the droplets and gel network in the emulsions. This finding would make sense to further understand the modification of emulsifying properties of alimentary proteins through glycosylation with saccharides and develop novel protein-based emulsifiers.
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MOLECULES
·
2022
·Vol. 27
·期 8
·2571
·
IF 4.927
Zhiyuan Yang,Tong Ye,Fei Ma,Xinhong Zhao,Lei Yang,Guifang Dou,Hui Gan,Zhuona Wu,Xiaoxia Zhu,Ruolan Gu,Zhiyun Meng
Uncontrolled hemorrhage from trauma or surgery can lead to death. In this study, chitosan/kaolin (CSK) and chitosan/montmorillonite (CSMMT) composites were prepared from chitosan (CS), kaolin (K), and montmorillonite (MMT) as raw materials to control bleeding. The physiochemical properties and surface morphology of CSK and CSMMT composites were analyzed by Fourier transform infrared spectrometry (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), zeta potentials, and X-ray fluorescence (XRF). The hemostatic mechanism was measured in vitro by activated partial thromboplastin time (APTT), prothrombin time (PT), in vitro clotting time, erythrocyte aggregation, and thromboelastogram (TEG). The hemostasis ability was further verified by using tail amputation and arteriovenous injury models in rats. The biocompatibility of CSK and CSMMT was evaluated by in vitro hemolysis, cytotoxicity assays, as well as acute toxicity test and skin irritation tests. The results show that CSK and CSMMT are promising composite materials with excellent biocompatibility and hemostatic properties that can effectively control bleeding.Keywords:chitosan;kaolin;montmorillonite;hemostatic material;hemorrhage control
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CARBOHYDRATE POLYMERS
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2022
·
IF 10.723
Lingyuan Chen,Jiali Tang,Suoque Wu,Shixing Wang,Zhaogang Ren
A pyridine-modified chitosan ( PMCS ) adsorbent with excellent adsorption performance and specific selectivity for Au(III) in wastewater was prepared. FTIR, SEM, XPS and XRD were used to study its adsorption performance, and various models were used to fit and analyze the experimental adsorption data. The maximum adsorption capacity of PMCS for Au(III) is 549.41 mg/g (298 K, pH = 4). The data analysis results prove that the PSO and Langmuir models best describe the adsorption process. Adsorption experiments in the presence of multiple ions proved that PMCS is selective for the adsorption of Au(III). The adsorption and desorption experiments showed that the adsorption rate of PMCS could still reach 94.77% after three cycles. In summary, PMCS was demonstrated to be a high-quality material for the adsorption of Au(III) from wastewater due to its excellent adsorption performance, specific selectivity and reusability.
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