MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Haohao Chen,Min Yuan,Fengqin Yin,Hui Cao,Xiuxiu Wu,Liling Hao,Weiyan Shi,Tai Ye,Fei Xu
The traditional nucleic acid-modified electrode in room-temperature manner suffers from the drawback of time-consuming and uneven distribution. In this work, we adopted the freeze-thawing method to construct a nicking enzyme-powered DNA walking machine on the gold electrode. In the absence of kanamycin, the intact substrate strand can trigger the self-assembly and form G4 nanowires on the electrode surface. After adding hemin, a significant catalytic current of the TMB oxidization can be observed. By contrast, once the specific binding between aptamer and kanamycin occurs, the activated walking strand processes a consecutive binding-cleavage cycle with the substrate strand on the electrode surface. The residue substrate fragment is unable to trigger the subsequent G-quadruplex assembly, resulting in a decrease in catalytic current. Under optimal experimental conditions, a linear relationship between the kanamycin concentration and current decrease was obtained in the range from 5 to 100 pM, and the limit of detection was 1.44 pM. This method has also demonstrated practical performance in the spiked milk and honey with satisfactory recoveries of 107.1 % to 114.3 % and 94.3 % to 101.5 %, respectively.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
Micro & Nano Letters
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2025
·Vol. 20
·期 1
·e70008
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IF 2.4
Xiuping Shen,Aiyong Zhu,Yafeng Xu
Doxorubicin (DOX), an anthracycline antibiotic, is widely used to treat a range of solid tumours and haematological malignancies. However, its clinical application in breast cancer is hindered by toxic side effects and the development of multidrug resistance (MDR). Enhancing the selective targeting of DOX and overcoming MDR are critical to improving treatment efficacy. Here, we present a DNA nanoflower (DNF)-based delivery system, designed via rolling circle amplification and multi-primer amplification (MCA), which co-delivers antisense oligonucleotides (ASOs) and DOX to human breast cancer cells (MCF-7). This system, named DNF-ASO@DOX, effectively promotes gene silencing, enhances drug accumulation and significantly inhibits cell proliferation. Furthermore, in vivo studies using mouse models of breast cancer demonstrated potent therapeutic effects, highlighting DNF-ASO@DOX as a promising strategy for enhanced anti-tumour therapy.
研究领域:
生物医学工程
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肿瘤学
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分子生物学
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药理学
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DOI
高影响因子
RSC Advances
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2017
·Vol. 7
·期 89
·56289-56295
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IF 3.108
Baozhu Zhang,Chunying Wei
A novel turn-on Hg2+ sensor was constructed based on fluorescent C–Hg2+-aptamer DNA-stabilized Ag nanoclusters (DNA–AgNCs), and was used to determine the concentration of Hg2+ over the range 2–18 nM with a detection limit as low as 0.25 nM. The sensing assay relied on a target-induced conformational transition of Hg2+-aptamer DNA. The conformational change of Hg2+-aptamer DNA from a single strand to a hairpin DNA due to the formation of a T–Hg2+–T complex in the presence of Hg2+ made the two darkish DNA–AgNCs approach each other closely and then enhanced the fluorescence of the AgNCs, which enabled the sensitive and specific detection of Hg2+. The proposed sensor was found to be easy to use, and allowed for the sensitive, selective, and turn-on detection of Hg2+. Furthermore, this approach has also been successfully applied to the detection of Hg2+ in real water samples, so this sensor may find application in monitoring Hg2+ in environmental samples.
研究领域:
分析化学
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环境科学
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生物传感
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DOI
高影响因子
Royal Society Open Science
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2018
·Vol. 5
·期 8
·
IF 2.504
Yuting Yan,Yanwei Cao,Chunsheng Xiao,Yang Li,Xiaoxuan Xiang,Xinhua Guo
Herein, the DNA strands containing 5′-(CGA)n and consecutive guanines are used to construct supramolecular DNA nanostructures that are size-controlled by pH values. Additionally, the introduction of thymine linkers within DNA nanostructures is necessary to maintain the stability of long-sized nanostructures. This work also demonstrates a method for accurately building DNA nanostructures.
研究领域:
纳米技术
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分子生物学
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超分子化学
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DOI
PubMed
高影响因子
LIFE SCIENCES
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2024
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IF 5.2
Yi Xiao,Jiahe Zhang,Xinran Li,Pinxuan Liu,Bo Gou,Zeyu Gao,Moshi Song
Background The role of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is multifaceted, paradoxically promoting both cell survival and cell death across multiple organs. However, its impact on lung homeostasis remains elusive. Here, we investigate the function of DNA-PKcs in mouse lungs, aiming to elucidate its role for lung abnormalities associated with DNA-PKcs deficiency. Materials and methods Histological assessment and immunohistochemistry were used to reveal the pathological changes of the lungs in DNA-PKcs-deficient mice. Transcriptomic analysis identified differentially expressed genes and pathways in DNA-PKcs-deficient lungs. Furthermore, mitochondrial dysfunction induced by DNA-PKcs deficiency was investigated by qPCR and immunoblotting. Mouse primary lung fibroblasts were used to evaluate the potential therapeutic effect of inhibiting mitochondrial fission with Mdivi-1. Key findings In DNA-PKcs-deficient mouse lungs, we observed pathological changes including alveolar septal thickening, capillary congestion and hemorrhage, along with lung cell proliferation. Transcriptome analysis revealed an upregulation of the reactive oxygen species (ROS) biosynthesis process and the apoptotic signaling pathway caused by DNA-PKcs deficiency. Further investigations demonstrated that DNA-PKcs deficiency led to mitochondrial dysfunction and increased oxidative stress, along with increased cell apoptosis in the mouse lungs. Notably, we detected enhanced phosphorylation of the mitochondrial fission protein DRP1 in DNA-PKcs-deficient mouse lungs. Intriguingly, inhibiting mitochondrial fission using Mdivi-1 suppressed cell death in primary mouse lung fibroblasts with siRNA-mediated DNA-PKcs knockdown. Significance Our study provides insights into the crucial role of DNA-PKcs in sustaining lung homeostasis via the maintenance of mitochondrial functionality and provides a therapeutic strategy targeting mitochondrial fission against DNA-PKcs deficiency-associated lung diseases.
研究领域:
分子生物学
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细胞生物学
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肺生理学
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病理学
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DOI
PubMed
高影响因子
Nano Today
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2024
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IF 13.2
Qizhi Liang,Qianxi Zhou,Hai Shi,Shuai Wu,Jiahua Yang,Tianyu Zeng,Jing Zhao,Yongmei Yin,Genxi Li
The controllable assembly of metal-organic frameworks (MOFs) nanomaterials is an effective means to enhance their application capabilities. In this paper, a novel method is reported to control MOFs assembly by using a DNA framework material, a tetrahedral DNA nanostructure (TDN) labeled with phosphate groups at the vertex. In particular, various well-defined superstructures can be tailored by regulating the number of phosphate groups that coordinate with catalytic Zr-MOFs to create polymeric MOFs superstructures (Poly(MOFs)), exhibiting excellent catalytic activities and molecular recognition ability as well as greatly amplified signal readout. Furthermore, based on the DNA framework-controlled Poly(MOFs), a colorimetric biosensor with excellent analytical performance is fabricated for improving the diagnosis of human epidermal growth factor receptor 2 (HER2)-positive breast cancer. It is demonstrated that the quantification of HER2 protein can be achieved at molecular level with the limit of detection (LOD) of 12 pg L -1 and cellular level with the LOD of 10 cells. More importantly, the detection of HER2 in the serum of breast cancer patients have been achieved, thus HER2-positive and triple-negative breast cancer patients can be well distinguished. Considering the substitutability of the target, this study shows great potential in biomedical research as well as clinical applications.
研究领域:
材料科学
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纳米技术
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生物医学工程
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癌症诊断
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分析化学
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2015
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IF 3.368
Linlin Wang,Shaopu Liu,Wanjun Liang,Dan Li,Jidong Yang,Youqiu He
A fluorescent “turn off–on” pattern for the detection of herring sperm DNA (hsDNA) had been designed through utilizing the interaction between polymyxin B sulfate (PMBS) and hsDNA as an inherent performance and the fluorescent transformation of glutathione (GSH)-capped CdTe quantum dots (QDs) as an external manifestation. Due to the occurrence of the photoinduced electron transfer from the QDs to PMBS, the fluorescence of GSH-capped CdTe QDs could be effectively quenched by PMBS, causing the system into “off” state. With the addition of hsDNA, the quenched fluorescence of GSH-capped CdTe QDs could be restored for the reason that PMBS embedded into hsDNA double helix structure to form new complex and peeled off from the surface of GSH-capped CdTe QDs, leading the system into “on” condition. Corresponding experimental results illustrated that the relative recovered fluorescence intensity of GSH-capped CdTe QDs–PMBS system was near proportional to the concentration of hsDNA within the range of 0.059–15.0 μg mL −1 . This proposed method demonstrated a good linear correlation coefficient of 0.9937 and a detection limit (3 σ / K ) of 0.018 μg mL −1 for hsDNA. This dual-directional fluorescent biosensor overcame the selectivity problem commonly existed in the traditional mono-directional fluorescence detection mode and owned perfect analysis applications in biochemical DNA monitoring.
研究领域:
生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
NUCLEIC ACIDS RESEARCH
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2024
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IF 16.6
Huang Shen,Lou Yongliang,Zheng Laibao
CRISPR-Cas12a, an RNA-guided nuclease, has been repurposed for genome editing and molecular diagnostics due to its capability of cis-cleavage on target DNA and trans-cleavage on non-target single-strand DNA (ssDNA). However, the mechanisms underlying the activation of trans-cleavage activity of Cas12a, particularly in the context of split DNA activators, remain poorly understood. We elucidate the synergistic effect of these activators and introduce the concepts of induced targeting effect and exon-unwinding to describe the phenomenon. We demonstrate that upon binding of split DNA activators adjacent to the Protospacer Adjacent Motif (PAM) to the Cas12a ribonucleoprotein (Cas12a–RNP), a ternary complex form that can capture and interact with distal split DNA activators to achieve synergistic effects. Notably, if the distal activator is double-strand DNA (dsDNA), the complex initiates exon-unwinding, facilitating the RNA-guide sequence's access. Our findings provide a mechanistic insight into action of Cas12a and propose a model that could significantly advance our understanding of its function.
研究领域:
分子生物学
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基因编辑
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诊断学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2014
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IF 6.451
Qiong Hu,Weiwen Hu,Jinming Kong,Xueji Zhang
In this work, we presented a novel signal amplification approach to construct an electrochemical DNA biosensor for the ultrasensitive determination of sequence-specific DNA by exploiting hematin as biomimetic catalyst toward in situ metallization . Briefly, thiolated peptide nucleic acid (PNA) probes were firstly immobilized onto gold electrode through the formation of self-assembled monolayer (SAM) and then hybridization was accomplished in the ensuing step. After that, hematin molecules were introduced to the hybridized PNA/DNA heteroduplexes by employing phosphate–zirconium–carboxylate coordination chemistry . Next, the attached hematin molecules acted as catalyst in accelerating the reduction of silver ions in the presence of catechol , leading to the in situ deposition of silver particles onto the electrode. Finally, the deposited silver particles were electrochemically stripped into KCl solution and measured by square wave voltammetry (SWV). Under optimal conditions, the hematin-based electrochemical DNA biosensor presented a good linear relationship between the stripping peak currents and logarithm of single-stranded DNA (ssDNA) concentrations in the range from 0.1 fM to 0.1 nM with a low detection limit of 62.41 aM, and it rendered satisfactory analytical performance for the determination of ssDNA in serum samples. Furthermore, it exhibited good reproducibility and stability, meanwhile, it also showed excellent specificity toward single-nucleotide polymorphism (SNP). Therefore, the hematin-based signal amplification approach has great potential in clinical applications and is also suitable for quantification of biomarkers at ultralow level.
研究领域:
生物化学
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分析化学
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分子生物学
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DOI
PubMed
高影响因子
OPTICAL MATERIALS
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2020
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IF 2.779
Zhenhua Li,Guotao Sun,Christopher D. Snow,Yanan Xu,Yao Wang,Dan Xiu,Yu Zhang,Zhijun Zhu,Laurence A. Belfiore,Jianguo Tang
DNA-based high-performance rare-earth materials were synthesized for the first time to enhance the near-infrared fluorescence of Yb 3+ and to provide a sensory response to Cu 2+ and Fe 3+ . Yb 3+ , TTA, phen and DNA were coordinated in an ethanol solution. The mixture produced a stable DNA material under the action of stirring and ultrasound. By changing the ratio, the morphology and luminous properties of the product were controlled. The addition of DNA increased the luminescent intensity of Yb 3+ complexes three folds. Earlier reports investigated Yb 3+ complexes fluorescence in the visible range or reported weak near-infrared emission. The rare-earth materials we synthesized herein have significantly increased near-infrared fluorescence intensity of Yb 3+ , and significantly improved biocompatibility. Potential applications for DNA-coordinated Yb 3+ complexes include the detection of Cu 2+ and Fe 3+ in vitro and in vivo , taking advantage of good penetration of IR through human tissue in the window 950 nm to 1050 nm.
研究领域:
材料科学
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分析化学
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生物化学
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DOI
高影响因子
Science of Advanced Materials
·
2019
·Vol. 11
·期 5
·680-684
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IF 1.158
Ji Chenyang,Jiang Pengfei,Ye Xiufang,Chang Menglei,Liu Fuhua,Shen Yuanyuan,Chen Dongchu,Nie Libo
Researchers pay close attentions to suspension arrays in high-throughput bioassays, in which biomolecules are detected in a solution with advantages such as convenient separation, flexibility in target selection, higher quality of results, etc. In this study, we designed a novel suspension
DNA detection based on magnetic nanoparticles and quantum dot (QD) encoded microbeads, in which detection signals were amplified by bio-barcode technology. Two different QDs were integrated into polystyrene (PS) microspheres that were used as supports for different DNA sequences. A FITC labeled
DNA was designed as reporting signal probe and fluorescence signal was amplified by the bio-barcode technology. Results indicated that the fluorescence wavelengthes for the QD encoded microbeads and fluorescence labels could be distinguished without interference. The target DNAs at concentrations
about 50 nm were effectively detected, and the ratio of background-subtracted fluorescence intensities for complementary DNA to single-base mismatched DNA was 4.15:1. This suspension system can be extended to suspension array for detection of biomolecules such as DNAs and proteins.
研究领域:
生物化学
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分子生物学
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DOI
高影响因子
CURRENT APPLIED PHYSICS
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2015
·
IF 2.212
Hongyan Zhang,Zhenhong Jia,Xiaoyi Lv
A technique is demonstrated to detect DNA hybridization based on surface layer of Au/porous silicon microcavity (Au/PSM) substrate for very small amount of biomolecules. Simulations show that the increase of effective refractive index for the first layer of PSM will cause a blue shift for its reflectance spectrum, and the blue shift becomes less with the increase of refractive index for one more layers. In experiments, such a blue shift of reflectance spectrum of PSM comes from the increase of refractive index by DNA hybridization on the surface. The detection limit of Au/PSM biosensor is 15.15 nM for 19-base pair DNA, which is comparable to that of reported biosensors based on porous silicon (PS). Therefore such an Au/PSM could be very useful to develop simple, rapid and sensitive optical biosensors when the amount of target is very small.
研究领域:
生物医学工程
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光学传感
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分子生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）