Analytical Methods
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2023
·
IF 3.1
Huanzong Zhang,Binhuang Cai,Fan Cai,Mingzhe Lian,Yinghui Wang
Gastric cancer is a prevalent malignant tumor of the gastrointestinal tract accompanied by a high mortality rate; therefore, early gastric cancer screening is critical for improving patient survival. In this study, we present a facile fluorescence immunoassay for highly sensitive screening of pepsinogen I (PG I) based on a one-pot biomimetic mineralization process for the synthesis of gold nanocluster-anchored manganese dioxide (AuNCs/MnO2) nanosheets. MnO2 first quenches the fluorescence of AuNCs through the Förster resonance energy transfer effect, whereas the introduction of ascorbic acid (AA) leads to the decomposition of MnO2 and rapidly recovers the fluorescence of AuNCs. Based on the above principles and phenomena, we developed a sensitive fluorescence immunoassay for the in situ generation of AA to detect PG I. Specifically, in the presence of PG I, the sandwich-type immunoreactivity-enriched alkaline phosphatase-labeled secondary antibody catalyzes the production of AA from the substrate, which enhances the fluorescence intensity. Under optimized conditions, the fluorescence intensity increased linearly with the concentration of PG I (0.05 to 200 ng mL−1) with a limit of detection (LOD) of 0.013 ng mL−1 (S/N = 3). The designed sensing platform has good stability (more than one year) and excellent anti-interference capability and demonstrates satisfactory accuracy for detection in real samples compared to commercial ELISA kits.
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ACS Omega
·
2023
·
IF 4.1
Kun Luo,Ya Li,Tong Liu,Xiangqun Zhuge,Etelka Chung,Andrew R. Timms,Simon P. Graham,Guogang Ren
Copper nanoparticles (CuNPs) and gold nanoclusters (AuNCs) show a high catalytic performance in generating hydrogen peroxide (H2O2), a property that can be exploited to kill disease-causing microbes and to carry carbon-free energy. Some combinations of NPs/NCs can generate synergistic effects to produce stronger antiseptics, such as H2O2 or other reactive oxygen species (ROS). Herein, we demonstrate a novel facile AuNC surface decoration method on the surfaces of CuNPs using galvanic displacement. The Cu–Au bimetallic NPs presented a high selective production of H2O2 via a two-electron (2e–) oxygen reduction reaction (ORR). Their physicochemical analyses were conducted by scanning electron microscopy (SEM), transmitting electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). With the optimized Cu–Au1.5NPs showing their particle sizes averaged in 53.8 nm, their electrochemical analysis indicated that the pristine AuNC structure exhibited the highest 2e– selectivity in ORR, the CuNPs presented the weakest 2e– selectivity, and the optimized Cu–Au1.5NPs exhibited a high 2e– selectivity of 95% for H2O2 production, along with excellent catalytic activity and durability. The optimized Cu–Au1.5NPs demonstrated a novel pathway to balance the cost and catalytic performance through the appropriate combination of metal NPs/NCs.
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Solar RRL
·
2023
·Vol. 7
·期 20
·2300369
·
IF 7.9
Haoyu Wang,Shuanghong Wu,Haolin Zhang,Xianghua Yao,Furong Wang,Peng Huang,Xiaodong Liu
The light-soaking (LS) effect has been reported to limit the accuracy and stability of device power output of perovskite solar cells (PSCs) and it is significant to develop effective approaches and strategies to eliminate the LS effect in PSCs. There are very few reports on suppressing the LS effect in CsPbI 2 Br-based all-inorganic PSCs. Herein, a method to eliminate the LS effect by synchronous passivation of anionic and cationic defects in CsPbI 2 Br perovskite film is demonstrated. The 4-(chlorosulfonyl)benzoic acid (CSBA) is added into perovskite precursor and the in situ generation of I 3 − effectively passivates the anionic halogen vacancy defects in CsPbI 2 Br. In the meantime, the Lewis base groups on CSBA coordinate with Cs + and Pb 2+ to passivate the cationic defects. This zwitterionic passivation effect of CSBA remarkably cuts off the path of defect-induced charge recombination and thus eliminates the LS effect. This work offers a deep opinion for future researchers to modulate the LS effect of all-inorganic PSCs.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2023
·Vol. 26
·期 3
·2472-2477
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IF 3.3
Yingui Gao,Zhihuang Xu,Liwang Ye,Yuanjie Wang,Xinxin Zhuang
Lead-free metal halides are considered as alternatives to lead-based perovskites due to their low toxicity, rich structural diversity, and high luminescence properties. We report millimeter-sized single crystals of a new zero-dimensional (0D) copper(I)-based hybrid material, (AEP)2Cu2Br6·2Br·2H2O (AEP = C6H18N33+), which exhibits bright broadband green photoluminescence (PL) at 510 nm with a Stokes shift of 220 nm and a PL lifetime of 121.1 μs. Density functional theory (DFT) calculations and experimental studies reveal that the green light can be attributed to self-trapping exciton (STE) emission. It is worth mentioning that this crystal has a high photoluminescence quantum yield (PLQY) of 90.5%, which is higher than most copper halides.
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Small
·
2023
·Vol. 19
·期 49
·2304644
·
IF 13.3
Ping Lu,Zhaoyang Qi,Jie Chen,Changshen Ye,Ting Qiu
High-efficiency adsorption of aromatic sulfur-containing compounds from liquid hydrocarbon fuels over metal-organic frameworks (MOFs) is challenging because of inert metal sites. A new method, the Ce-enhanced modulation of MOFs’ microenvironment, is proposed to modulate the –COO···Cu(II)– coordination microenvironment of Hong Kong University of Science and Technology (HKUST-1) using Ce(III) as a molecular scalpel for fabricating abundant high-efficiency Cu(I) and Cu-coordination-unsaturated sites and improving the pore structures around adsorptive sites. The optimal CH-250 thus exhibits adsorptive capacities for 20.2, 28.0, and 58.3 mg S g –1 of thiophene, benzo-thiophene, and dibenzothiophene, respectively, which are superior to most reported MOFs, zeolites, and nanoporous carbons. The constructed Cu(I) sites show stronger affinity for dibenzothiophene (−0.86 eV) than the initial Cu(II) (−0.74 eV) for out-of-plane adsorption. Further, they are far stronger in-plane adsorption interactions in DBT/CH-250 (−0.90 eV) than those in DBT/HKUST-1 (−0.37 eV). Thus, molecular engineering for modulating the coordination microenvironment of MOFs shows great potential for adsorption desulfurization.
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International Journal of Nanomedicine
·
2023
·
IF 8
Mingyuan Li,Meng Li,Xinyi Li,Wanhui Shao,Xiujuan Pei,Ruyue Dong,Hongmeng Ren,Lin Jia,Shiqin Li,Wenlin Ma,Yi Zeng,Yun Liu,Hua Sun,Peng Yu
Purpose In the present study, we prepared collagen liposomes with the addition of polyol, which is expected to not only increase the solubility of collagen but also improve skin penetration.Methods Collagen liposomes were prepared by the film dispersion method, and their characteristics, integrity and biosafety were evaluated by Fourier transform infrared spectroscopy (FTIR), UV-VIS spectroscopy, polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS) and transmission electron microscope (TEM). The transdermal absorption of collagen and collagen liposomes were tested by an ex vivo horizontal Valia-Chien diffusion cell system.Results We first demonstrated that collagen extracted from bovine Achilles tendon was type I collagen. The results of DLS measurement and TEM observation showed that the collagen liposomes were spherical in shape with average diameter (75.34±0.93 nm) and maintained high stability at low temperature (4°C) for at least 42 days without toxicity. The encapsulation rate of collagen liposomes was 57.80 ± 0.51%, and SDS-PAGE analysis showed that collagen was intact in liposomes. Finally, permeability studies indicated that the collagen-loaded liposomes more easily penetrated the skin compared to collagen itself.Conclusion This study proposed a new method to improve the bioavailability and permeability of bovine type I collagen, which improves the applicability of collagen in biomedicine, cosmeceuticals and pharmaceutical industries.
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Inorganic and Nano-Metal Chemistry
·
2019
·
IF 0.685
Xue-Wen Liu,Jie Huang,Yu-Xuan Tang,Song-Bai Zhang,Ji-Lin Lu
Two new ruthenium complexes containing a new polyaza ligand were found to inhibit the DNA supercoiled relaxation mediated by topoisomerase I (topo I), cleave DNA under irradiation and interact with CT-DNA by intercalation. Both complexes exhibited fast singlet oxygen generation rate, and DNA photocleavage reaction should be initiated by 1O2. Furthermore, complex 2 showed higher photocleavage activity, topo I inhibition activity, and DNA affinity than complex 1. This work shows that two complexes can act as DNA binders with high affinity and good DNA photocleavers. Furthermore, complex 2 can act as a good topo I inhibitor.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Daqin Chen,Junni Li,Xiao Chen,Jiangkun Chen,Jiasong Zhong
Currently, metal halide perovskite nanocrystals have been extensively explored due to their unique optoelectronic properties and wide application prospects. In the present work, a facile grinding method is developed to prepare whole-family APbX3 (A = MA, FA, and Cs; X = Cl, Br, and I) perovskite nanocrystals. This strategy alleviates the harsh synthesis conditions of precursor dissolution, atmosphere protection, and high temperature. Impressively, the as-prepared perovskite nanocrystals are evidenced to have halogen-rich surfaces and yield visible full-spectral emissions with maximal photoluminescence quantum yield up to 92% and excellent stability. Additionally, the grinding method can be extended to synthesize widely concerned Mn2+-doped CsPbCl3 nanocrystals with dual-modal emissions of both excitons and dopants. As a proof-of-concept experiment, the present perovskite nanocrystals are demonstrated to be applicable as blue/green/red color converters in UV-excitable white-light-emitting diodes.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Jintao Xue,Yongli Shi,Chunyan Li,Xiaojie Xu,Shuxin Xu,Mingxiang Cao
The purpose of this study was to fabricate a novel binary hydrogel, and the insulin-loaded hydrogel was used as rectal suppository to prevent type I diabetes. The binary hydrogel was synthesized via solution polymerization. Its structure was studied by Fourier transform infrared spectroscopy (FTIR) and Raman spectra. The swelling behaviors of binary hydrogels were revealed in pH 1.2, 6.8 and 7.4 buffers, respectively. Their inner morphologies were observed with a scanning electron microscope (SEM). Insulin (INS) was selected as a model drug and encapsulated into the binary hydrogels. INS release study was carried out in pH 7.4 buffer. The hypoglycemic effects of INS-loaded hydrogels were studied by rectal administration. FTIR and Raman spectra confirmed the obtaining of binary hydrogels. The hydrogel showed a high swelling ratio in pH 7.4 (rectum environment). SEM photographs illustrated that many micro-pores in the inner of binary hydrogels, which could accommodate abundant guest molecule ( e.g. INS). INS release profile suggested that INS-loaded hydrogels could diffuse INS at a sustained manner. Animal studies proved that INS-loaded binary hydrogel had an obvious hypoglycemic effect. Therefore, it could be speculated that the binary hydrogel had a potential application on treating type I diabetes by rectal administration.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Jing Lin,Pengjuan Ni,Yujing Sun,Yilin Wang,Li Wang,Zhuang Li
In this work, a simple, highly selective and sensitive method for detecting biothiols based on I − -H 2 O 2 -3,3’,5,5’-tetramethylbenzidine (TMB) system is developed. The main determination mechanism of the colorimetric assay is that iodide could catalyze the oxidation of TMB in the presence of H 2 O 2 to form oxidized TMB (ox-TMB). With the introduction of biothiols, the biothiols cause the reduction of ox-TMB, accompanied with the color of the solution changing from the original blue to colorlessness. UV–vis absorption spectrum shows that the intensity of peak at 652 nm reduces largely. Other 20 kinds of amino acids have also been studied for proving the high sensitivity and selectivity of current approach. This colorimetric analysis method can be successfully applied to the practical sample determination with satisfactory results. The results indicated that this work is a facile, time-saving and cost-effective method for detecting biothiols.
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JOURNAL OF FOOD AND DRUG ANALYSIS
·
2022
·
IF 6.157
Jia Wang,Chengyi Hong,Zhengzhong Lin,Zhi-Yong Huang
A simple fluorescence method to quantitatively monitor malachite green (MG) was introduced based on the conformation switch effect of MG-aptamer (Apt) and the fluorochrome of SYBR Green I (SGI). SGI can embed in the stem-loop structure of Apt to produce fluorescence. However, the fluorescence will be quenched when MG is introduced because of the specific binding of Apt to MG. Under the optimal conditions, the fluorescence method displayed excellent linear relationship between the fluorescence quenching efficiencies and the MG concentrations ranging from 0.02 μmol L −1 to 1.0 μmol L −1 . The detection limit of the method was 0.67 μg kg −1 (3 α / κ , n = 9) which is lower than the limit (1 μg kg −1 ) specified in the national standard of China (GB/T 19857–2005) and the limit (2 μg kg −1 ) specified by the European Food Safety Authority (EFSA, 2016). Excellent recoveries between 87.1% and 106.9% tested by spiking standards were achieved with RSD less than 6.4%. The data were confirmed by the method of HPLC, and the results proved that the established fluorescence method could be used for the rapid detection of MG in fish with high accuracy and sensitivity.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 11
·5330-5337
·
IF 3.3
Yu Wang,Rao Chen,Dongmei Liu,Chaofei Peng,Junwei Wang,Xiaoqing Dong
The development of effective Vis-sensitive photoinitiators for light-emitting diode (LED) polymerization is still an emerging challenge as LEDs are being developed into the predominant light sources. In this work, a series of new functionalized photoinitiators, containing thioxanthones and oxime-esters as functional groups, was designed and straightforwardly synthesized in two steps. The structures of the photoinitiators were studied using 1H NMR, 13C NMR, and high-resolution mass spectrometry. The conclusion of the photophysical properties indicated that the long wavelength absorptions from 360 nm to 405 nm made the investigated thioxanthone derivatives quite appealing as a valuable photoinitiator under UV-Vis LEDs. The structure–reactivity relationship was also studied in detail through real-time FTIR spectroscopy. A possible photolysis mechanism was expected based on further photolysis and online 1H NMR activities. With a proper molecular design, the new functionalized photoinitiators were synthesised straightforwardly and they displayed more advanced initiation abilities than their parent thioxanthone.
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