APPLIED CATALYSIS B-ENVIRONMENTAL
·
2024
·
IF 20.2
Tiantian Lu,Ying Liu,Youzeng Li,Jialei Chen,Shan Chen,Xuelong Liao,Wei Xia,Tong Zhou,Wei Wang,Zhuo Chen,Rong Huang,Huan Wang
Cobalt single atom catalysts (Co SACs) have been extensively explored in catalyzing the two-electron oxygen reduction reaction (2e – ORR) into hydrogen peroxide (H 2 O 2 ). However, the contribution of active sites for 2e – ORR in different media remains elusive. Here, we synthesized epoxy-modified Co SACs through a chemical vapor deposition approach followed by an oxidation treatment, which provides a model catalyst to identify the distinct role of Co sites and oxygen species for 2e – ORR. It is found that Co sites dominantly function in acidic media and barely works in neutral solution. Introduction of epoxy groups in the vicinity of Co sites can enlarge interlayer spacing and reinforce Co–N strength, which facilitates O 2 transport and weakens Co–*OOH interaction, collectively contributing to a superior selectivity for acidic H 2 O 2 generation. While for neutral 2e – ORR, the O-containing groups distant from Co sites serve as the dominant active sites. Encouragingly, the onsite production of H 2 O 2 in the acidic solution can be efficiently utilized for pollutant removal.
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Ting Feng,Jiani Shi,Jiaojiao Xia,Xuemei Ren,Onyinye Idowu Adesanya,Hongbo Suo,Bin Zou
In this study, the critical importance of designing an appropriate immobilized carrier and method for free lipase to ensure exceptional biological catalytic activity and stability was emphasized. Covalent organic frameworks (COF-1) were synthesized as a novel porous carrier with an azine structure (-C N-N C-) through the condensation of hydrazine hydrate and benzene-1,3,5-tricarbaldehyde at room temperature. Simultaneously, Rhizomucor miehei lipase (RML) was immobilized within the COF-1 carrier using an in-situ aqueous phase method. Characterization of the carrier and RML@COF-1 and evaluation of the lipase properties of RML and RML@COF-1 through p -Nitrophenyl palmitate hydrolysis were conducted. Additionally, application in the synthesis of 1, 3-dioleoyl-2-palmitoylglycerol (OPO) was explored. The results showed that RML@COF-1 exhibited a high enzymatic loading of 285.4 mg/g. Under 60℃ conditions, the activity of RML@COF-1 was 2.31 times higher than that of free RML, and RML@COF-1 retained 77.25% of its original activity after 10 cycles of repeated use, indicating its excellent thermal stability and repeatability. Under the optimal conditions (10%, 1:8 PPP/OA, 45℃, 5 h), the yield of OPO reached 47.35%, showcasing the promising application prospects of the novel immobilized enzyme synthesized via in-situ aqueous phase synthesis for OPO preparation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Linghui Kong,Sobia Kousar,Kai Sun,Runxing Sun,Yuewen Shao,Chao Li,Yuchen Jiang,Lijun Zhang,Shu Zhang,Xun Hu
Aliphatic organics are reactive components of biomass in activation, playing important roles in pore development. They could be consumed with progress of activation while introducing external O 2 might create additional aliphatic structures via oxidation, probably facilitating generation of extra pores in resulting activated carbon (AC). This was investigated herein by activation of spring jasmine wood with ZnCl 2 or K 2 C 2 O 4 in presence of 11 % or 5 % O 2 at 550 or 700 °C. The results suggested that the oxygen added diminished yield of AC O 2 ZnCl 2 by 21.7 % and yield of AC O 2 K 2 C 2 O 4 by 29.0 %, resulting from partial oxidation of carbonaceous organics on nascent AC. Nonetheless, O 2 presence promoted pore development, increasing S BET from 1833.8 m 2 g -1 for AC N 2 -ZnCl 2 to 2018.2 m 2 g -1 for AC O 2 ZnCl 2 . The S BET of AC O 2 K 2 C 2 O 4 also increased by 35.6 % with presence of O 2 . Oxidization of nascent AC generated more oxygen-containing species that actively involved in polymerization with ZnCl 2 or cracking with K 2 C 2 O 4 to form more pores. Additionally, O 2 presence generated smaller pores in AC O 2 ZnCl 2 while larger ones in AC O 2 -K 2 C 2 O 4 , enhancing the latter AC for adsorption. Oxidation reactions could also form the AC with oxygen-rice surface and hydrophilic nature, which negatively affect aromatization and reduced interplanar spacing of carbon crystals in AC.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Zhao Huan,Hu Xueli,Guan Shenghong,Cai Jinhong,Li Xiaohan,Fang Jiaxi,Lin Bo,Zhu Wei,Tian Jingkui,Jin Juan,He Qiang,Zhang Xiaoyong
Introduction: Some herbal ingredients can reshape the composition of the gut microbiome as well as its metabolites. At the same time, the gut microbiota can also affect drug metabolism. A large number of studies have reported that saponins are biotransformed under the action of intestinal microorganisms to improve drug efficacy and bioavailability. Capilliposide A is a triterpenoid saponin, which is derived from Lysimachia capillipes Hemsl. CPS-A has anti-inflammatory pharmacological activity, but the substance basis in vivo is unknown at present, so studies on the interaction between intestinal microorganisms and CPS-A may clarify the pharmacodynamic substance basis of CPS-A.Methods: This study established a colitis mouse model, collected sterile feces from normal mice and colitis mice, and incubated CPS-A with two different intestinal flora in vitro. Based on LC-MS, the metabolic process of CPS-A mediated by intestinal microbes and the intervention effect of CPS-A on intestinal microbiome derived metabolites were studied.Results: The results of experiments indicate that intestinal microorganisms can mediate the biotransformation of CPS-A and metabolize it into corresponding deglycosylation products, thereby promoting its drug effect. Not only that, CPS-A can also promote metabolites such as Deoxycholic acid, Histamine, 3-Hydroxytridecanoic acid, and Indole-3-acetic acid in the intestinal microbiota of mice with colitis. This may result in anti-colitis effects. CPS-A mainly involved in metabolic pathways such as azathioprine and mercaptopurine, which may also have beneficial or adverse effects.Discussion: This study on the interaction between CPS-A and microbiota provides a new idea for the study of traditional Chinese medicine with poor oral bioavailability. The regulatory effect of CPS-A on the metabolites of intestinal flora in colitis mice was also found. It laid a foundation for exploring the mechanism of action of saponins on colitis mice.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIVES OF TOXICOLOGY
·
2018
·Vol. 92
·期 4
·1421-1434
·
IF 5.728
Jiang Shengwei,Lin Yuchun,Yao Huan,Yang Chuanli,Zhang Liyin,Luo Bing,Lei Zhao,Cao Liwei,Lin Naibo,Liu Xiangyang,Lin Zhongning,He Chengyong
Unfolded protein response (UPR) and endoplasmic reticulum (ER)-phagy are essential for cell homeostasis. Quantum dots (QDs), which have been widely used for biomedical applications, can accumulate in the kidney tissues and may cause renal dysfunction. However, the molecular mechanism of QDs-induced nephrotoxicity is still obscure. The present study was aimed to elucidate the role and mechanism of UPR and ER-phagy in QDs-induced nephrotoxicity. Herein, human embyronic kidney (HEK) cells were exposed to 15, 30, 45, and 60 nM cadmium telluride (CdTe)-QDs for 12 and 24 h. And CdTe-QDs (30–60 nM) inhibited the HEK cell viability. The clathrin-dependent endocytosis was determined as the main pathway of CdTe-QDs cellular uptake. Within cells, CdTe-QDs disrupted ER ultrastructure and induced UPR and FAM134B-dependent ER-phagy. Blocking UPR with inhibitors or siRNA rescued the FAM134B-dependent ER-phagy, which was triggered by CdTe-QDs. Moreover, suppression of UPR or FAM134B-dependent ER-phagy restored the cell vability. In vivo, mice were intravenously injected with 8 and 16 nmol/kg body weight CdTe-QDs for 24 h. Kidney was shown as one of highest distributed organs of CdTe-QDs, resulting in renal dysfunction, as well as UPR and FAM134B-dependent ER-phagy in it. Thus, for the first time, we demonstrated that ER-phagy can be triggered by nanomaterials both in vitro and in vivo. In addition, blocking of UPR and ER-phagy showed protective effects against CdTe-QDs-induced toxicity in kideny cells. Notably, a secreted alkaline phosphatase reporter gene system has been developed as a sensitive and rapid method for evaluating the ER quality under the exposure of nanomaterials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 22
·3709
·
IF 4.7
Mengjun Hu,Zhuoan Zhou,Chenqi Liu,Zeqiang Zhan,Yan Cui,Shoukui He,Xianming Shi
Two-component systems (TCS) of Salmonella enterica serovar Enteritidis are composed of a histidine kinase and a response regulator (RR) and represent a critical mechanism by which bacteria develop resistance to environmental stress. Here, we characterized the functions of RRs in TCS in the formation of stress tolerance, motility and biofilm using twenty-six S . Enteritidis RR-encoding gene deletion mutants. The viability results unraveled their essential roles in resistance to elevated temperature (GlrR), pH alterations (GlrR, TctD, YedW, ArcA and YehT), high salt (PhoB, BaeR, CpxR, PhoP, UvrY and TctD), oxidative stress (PhoB, YedW, BaeR, ArcA, PhoP, UvrY, PgtA and QseB) and motility (ArcA, GlnG, PgtA, PhoB, UhpA, OmpR, UvrY and QseB) of S . Enteritidis. The results of the crystal violet staining, microscopy observation and Congo red binding assays demonstrated that the absence of ArcA, GlnG, PhoP, OmpR, ZraR or SsrB in S . Enteritidis led to a reduction in biofilms and an impairment in red/dry/rough macrocolony formation, whereas the absence of UvrY exhibited an increase in biofilms and formed a brown/smooth/sticky macrocolony. The results indicated the regulatory effects of these RRs on the production of biofilm matrix, curli fimbriae and cellulose. Our findings yielded insights into the role of TCSs, making them a promising target for combating S . Enteritidis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2021
·
IF 5.811
Yang Wenning,Jiang Xiaoquan,Liu Jingtong,Qi Dongying,Luo Zhiqiang,Yu Guohua,Li Xueyan,Sen Muli,Chen Hongjiao,Liu Wei,Liu Yang,Wang Guopeng
The study of screening active constituents from traditional Chinese medicine (TCM) is important for explicating the mechanism of action of TCM and further evaluating the safety and efficacy effectively. However, detecting and identifying the active constituents from complicated biological samples still remain a challenge. Here, a practical, quick, and novel integrated strategy from in vitro, in situ, in vivo to in silico for rapidly screening the active constituents was developed. Firstly, the chemical profile of TCM in vitro was identified using UPLC-Q Exactive-Orbitrap HRMS. Secondly, the in situ intestinal perfusion with venous sampling (IPVS) method was used to investigate the intestinal absorption components. Thirdly, after intragastric administration of the TCM extract, the in vivo absorbed prototype components were detected and identified. Finally, the target network pharmacology approach was applied to explore the potential targets and possible mechanisms of the absorbed components from TCM. The reliability and availability of this approach was demonstrated using Tongfengding capsule (TFDC) as an example of herbal medicine. A total of 141 compounds were detected and identified in TFDC, and among them, 64 components were absorbed into the plasma. Then, a total of 35 absorbed bioactive components and 50 related targets shared commonly by compounds and gout were integrated via target network pharmacology analysis. Ultimately, the effects of the absorbed components on metabolism pathways were verified by experiments. These results demonstrated that this original method may provide a practical tool for screening bioactive compounds from TCM treating particular diseases. Furthermore, it also can clarify the potential mechanism of action of TCM and rationalize the application of TFDC as an effective herbal therapy for gout.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Xuelin Wei,Juan Dai,Yuwei Du,Lei Liu,Ran Li,Zhiyun Wang,Lijun Wang,YuKun Huang,Pengfei Chen,Zheng Zhou,Xianggui Chen,Xiao Yang,Qin Wang
Many studies have shown that caffeic acid phenethyl ester (CAPE) has various functions, such as antioxidant, anti-inflammatory and anticancer activity, but its low bioavailability and stability limit its application. In this study, the colorectal targeted delivery system for CAPE based on a solid-in-oil-in-water (S/O/W) multilayer emulsion was prepared using CAPE-loaded nanoparticles as the solid phase, coconut oil as the oil phase, and a mixture of lecithin and sodium caseinate as the aqueous phase. The stability of the O/W interfacial layer was improved by using a sodium casein-lecithin mixture as the aqueous surface layer in the preparation. This S/O/W emulsion is a spherical droplet with an S/O/W trilayer structure with a particle size of 155.5 ± 0.72 nm and a polydispersity index (PDI) of 0.24 ± 0.01. The Fourier transform infrared (FTIR) results confirmed that CAPE was successfully loaded into the S/O/W emulsion. This S/O/W emulsion was able to maintain a stable liquid state at pH 6.00–7.4 or cholate concentration of 0–50 mg/mL but showed a gel state at pH 2.0–3.0. The storage experiments demonstrated that the S/O/W emulsion was stable for 15 days at 4 °C, but was prone to agglomeration and emulsion breakage at 25 °C. The in vivo digestion process indicated that the S/O/W emulsion was gradually digested in the digestive tract and released solid phase nanoparticles in the large intestine. Therefore, this newly developed targeted delivery system can effectively deliver CAPE to the colorectum and achieve a 12-hour delayed release, which improved the bioavailability and activity of CAPE.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL MONITORING AND ASSESSMENT
·
2025
·Vol. 197
·期 8
·1-13
·
IF 3
Luo Huili,Pan Xiaopeng,Yang Yixuan,Huang Yilu,Xiang Yuhang,Li Anxin,Zhao Jia
Bisphenol analogues (BPs), the endocrine-disrupting compounds, have been detected in water, sediment, and even agricultural products. Irrigation is the main way for material migration, and it has been suggested as one of the sources of BPs. But the attention to irrigation water and sediment, and further to the ecological risks, is rare. In a hilly irrigation area in southern China, the partition and accumulation, contribution sources, and potential ecological risk of four bisphenols (BPA, bisphenol S, F, and AF) in the irrigation canal were investigated in the spring of 2023 and 2024. All four BPs were detected in the irrigation water and sediment. BPF was the dominant of 19.6–60.9 ng/L in water, while BPA was still the prominent compound (46.2–54.4% of ∑BPs) in sediment. BPs in the irrigation canal were related to the direct discharge of aquaculture, domestic, and planting drainage. Although low risks for each compound, the comprehensive risk (∑RQ) of sediments went into medium risk. BPF and BPA were the main risk contributors. BPs in sediments increased steadily during the investigation. The special accumulative point was where slow flow maintained, especially caused by the hilly change. This comprehensive study of BPs in hilly irrigation canals should be valuable for reference in similar areas. And it will be beneficial for environmental protection and production safety.
研究领域:
环境科学
·
水污染研究
·
生态毒理学
·
农业环境管理
·
内分泌干扰物
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Yue Sun,Shuaiyi Ma,Yibo Liu,Zhibin Jia,Xiaodong Li,Lu Liu,Qian Ma,Kouadio Jean Eric-parfait Kouame,Chunmei Li,Youbin Leng,Shilong Jiang
The interfacial phospholipid and protein compositions of fat globule in human milk and three different infant formulas (F1: supplied with MFGM and OPO; F2: supplied with OPO and without MFGM; F3: supplied with MFGM and without OPO) were analyzed and compared, and the changes of interfacial composition and structure on their in-vitro infant gastrointestinal digestion were evaluated. The study from the interfacial properties of fat globule to illustrating the effect of interfacial composition and structure on lipolysis and proteolysis. The results showed that, both F1 and human milk had a similar interfacial phospholipid composition and higher sphingomyelin (SM) content. The interfacial protein composition of F1 was closer to human milk. F1 had the average particle size of 3.67 ± 0.12 μm and a phospholipid layer on the surface of triglyceride. Meanwhile, the relatively complete phospholipid ring structure in F1 could always be observed during gastric digestion, while was gradually destroyed during intestinal digestion. Moreover, F2 and F3 are significantly different from HM and F1 in composition and structure of fat globule. Although MFGM is added to commercial infant formula (F3), most MFGM still existed in the aqueous phase of emulsion in free form. Notable, F1 had similar in-vitro lipolysis and proteolysis properties to human milk. These results indicated that the simulation of human milk fat globule at the molecular level and structure feature was the key contributing to digestion behavior.
研究领域:
营养学
·
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 40
·期 2
·e70287
·
IF 1.7
Hao Cheng,Danrui Han,Lifang Xu,Longxiang Li,Donglai Ma,Pingping Chen,Xuhong Duan
Cnidii Fructus (CF) is a Chinese herbal medicine with bioactive immune properties and potential applications in treating ulcerative colitis (UC), but its well-defined bioactive constituents are understudied. The current study aimed to investigate CF's anti-UC bioactivity and elucidate its bioactive constituents via chromatographic analysis, in vitro/in vivo tests, and spectrum-effect analysis. Results showed 10 batches of CF inhibited the inflammatory response induced by lipopolysaccharide (LPS)–stimulated RAW 264.7 macrophages. High-performance liquid chromatography (HPLC) fingerprint profiles of the 10 batches were established, with 16 common peaks identified. Subsequently, 15 of these peaks (including eight reference-validated compounds) were characterized by ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS). Four anti-UC-related compounds were identified via spectrum-effect relationships, namely, diosmin, xanthotoxol, imperatorin, and osthole. Experimental verification confirmed these as key components potentially linked to alleviating inflammatory responses in UC. This study is the first to establish a spectrum-effect relationship for CF's anti-UC effects, identify its critical active constituents, and provide a scientific basis for CF quality control and UC therapeutic development.
研究领域:
生药学
·
天然产物化学
·
炎症性肠病
·
中药学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Jingyi Ye,Huiyi Wu,Chuanli Huang,Wanting Lin,Caifeng Zhang,Bei Huang,Banyi Lu,Hongyu Xu,Xiaoling Li,Xiaoying Long
Purpose The objective of this study was to compare the in vitro Fick’s first law, in vitro lipolysis, and in vivo rat assays for oral absorption of Biopharmaceutical Classification Systems Class II (BCS II) drugs in self-nanoemulsifying drug delivery system (SNEDDS), and studied drugs and oils properties effects on the absorption.Methods The transport abilities of griseofulvin (GRI), phenytoin (PHE), indomethacin (IND), and ketoprofen (KET) in saturated water solutions and SNEDDS were investigated using the in vitro Madin-Darby canine kidney cell model. GRI and cinnarizine (CIN) in medium-chain triglycerides (MCT)-SNEDDS and long-chain triglycerides (LCT)-SNEDDS were administered in the in vivo SD rat and in vitro lipolysis models to compare the oral absorption and the distribution behaviors in GIT and build an in vitro-in vivo correlation (IVIVC).Results In the cell model, the solubility of GRI, PHE, IND, and KET increased 6–8 fold by SNEDDS, but their permeability were only 18%, 4%, 8%, and 33% of those of their saturated water solutions, respectively. However, in vivo absorption of GRI-SNEDDS was twice that of the GRI suspension and those of CIN-SNEDDS were 15–21 fold those of the CIN suspension. In the lipolysis model, the GRI% in aqueous and pellet phases of MCT were similar to that in LCT. In contrast, the CIN% in the aqueous and pellet phases were decreased but that of the lipid phase increased. In addition, an IVIVC was found between the CIN% in the lipid phase and in vivo relative oral bioavailability (Fr).Conclusion The in vitro cell model was still a suitable tool to study drug properties effects on biofilm transport and SNEDDS absorption mechanisms. The in vitro lipolysis model provided superior oral absorption simulation of SNEDDS and helped to build correlation with in vivo rats. The oral drug absorption was affected by drug and oil properties in SNEDDS.
研究领域:
药理学
·
药物递送系统
·
生物药剂学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）