MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Yuanqian Xiong,Xiaoyu Gao,Yu Wang,Xiangrui Deng,Chunhong Xiong,Jinsheng Zhang,Qianhui Gu,Ganhui Huang
Norfloxacin (NOR) is now extensively used for the treatment of human and animal diseases. Unfortunately, large accumulations of NOR will pose a significant threat to the human body. Thus, it is urgent to develop a rapid and sensitive detection technique for the trace determination of NOR. In this study, an electrochemical molecular imprinting sensor with great properties was fabricated for the determination of NOR in milk and environmental water. The incorporation of bovine serum proteins produced a hybrid recognition interface consisting of molecularly imprinted polymers and peptides that provided good recognition properties for norfloxacin. The Fe 3 O 4 @ZIF-8 was distributed orderly on the electrode after the embedding of the magnets inside the electrode, then the BSA was coated on the electrode with the electrodeposited AuNPs through Au-S bonding. The sensor constructed in this study achieved ultra-sensitive detection of norfloxacin with an effective area of 0.08433 cm 2 , a limit of detection (LOD) of 0.108 pmol·L −1 , and a wide linear range (1 ∼ 100, 100 ∼ 1000 and 1000 ∼ 8000 pmol·L −1 ). The prepared sensors exhibited satisfactory performance in the detection of milk and environmental water, with acceptable recoveries ranging from 96.24 % to 104.88 %, and the relative standard deviation (RSD) was no more than 4.84 %.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Jiangnan Huang,Jiali Huang,Xugang Shu,Xingan Cheng,Hao Yu,Yonghai Cao,Zhuofeng Hu,Jimmy C. Yu
Ocean acidification from the uptake of CO 2 and antibiotic pollution from a consequence of inshore mariculture are two serious threats to marine ecosystems. In this work, a novelty oxygen-doping carbon nitride (O-CN) photocatalyst are designed for the release of CO 2 and degradation of a targeted antibiotic (ofloxacin, OFL) in acidified seawater. Notably, this photocatalytic system can take advantage of ocean acidification as more CO 3 •- radicals were produced in the seawater. The catalyst could degrade OFL rapidly ( k = 0.039 min −1 ) in the presence of CO 3 2– and HCO 3 – , which derive from the dissolved CO 2 in the seawater, much higher than that of pristine CN ( k = 0.013 min −1 ). Mechanism study revealed that the existence of CO 3 2– and HCO 3 – would facilitate the generation of new N-C-O (O1) groups on the O-CN surface. These O1 groups are the active sites for the formation of •OH radicals that can oxidize OFL and release CO 2 . This investigation offers a novel strategy for alleviating two environmental threats simultaneously.
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Biosensors-Basel
·
2025
·Vol. 15
·期 1
·30
·
IF 5.6
Zhaoyang Zhou,Xingyue Chen,Shuang Jiang,Zhuoer Chen,Sixian Wang,Yueyang Ren,Xiaodong Fan,Tao Le
Sulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human health. As a new recognition molecule, the aptamer can be recognized with SQX with high affinity and specificity, and the aptamer is no longer adsorbed to AuNPs after binding to SQX, which weakens the catalytic effect of AuNPs. Consequently, an aptasensor for the detection of SQX was successfully developed. This aptasensor exhibits a linear range of 40–640 ng/mL, with a detection limit of 36.95 ng/mL, demonstrating both sensitivity and selectivity. The recoveries of this aptasensor in water samples ranged from 90 to 109.9%, which was quite in line with high-performance liquid chromatography. These findings suggest that the aptasensor is a valuable tool for detecting SQX in aqueous environmental samples.
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DESALINATION
·
2024
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IF 8.3
Zhiyu Liu,Hui Wen,Shihai Jiang,Jiajie Xu,J. Paul Chen
Antibiotics are synthesized organic compounds for such applications as prevention of diseases. However, they have caused a series of ecotoxicological effects that can potentially be harmful to humans and living organisms. Nanofiltration (NF) can effectively remove antibiotics from many waste streams, such as pharmaceutical wastewater. NF membranes are always charged due to carboxyl and amino groups. This study aims to evaluate performance of negatively (nTFC), positively (pTFC), and dually (dTFC) charged NF membranes in treatment of several antibiotic compounds: ciprofloxacin, ofloxacin, tetracycline, amikacin, and erythromycin. The values of permeance were 26.1, 19.5, and 14.0 L/(m 2 ·h·bar) for nTFC, dTFC, and pTFC membrane, respectively. The dTFC membrane worked well in both permeability and treatment of the antibiotics. The solution pH greatly influenced the removal efficiency of the nTFC and pTFC membranes, while it had less effect on the dTFC membranes. The results demonstrated that dTFC membrane was more efficient than nTFC and pTFC membranes. It would have great potentials in treatment of such industrial effluent as antibiotics-containing wastewater. Our findings would provide a workable approach for fabrication of NF membranes for better treatment of antibiotics and other similar ones with higher efficiencies and greater selectivity.
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Analytical Methods
·
2024
·
IF 2.7
Wei-Chuang Kong,Chen-Chen Li,Ai-Hong Zhang,Xin-Long Li,Qian-Rui Gong,Bing-Tan Jin,Xiao-Juan Jia,Xu-Ying Liu,Yan-Fei Kang
Although enrofloxacin (ENR) is a widely used broad-spectrum antibiotic in veterinary medicine, its residues in animals can pose a risk to human health. Thus, we developed a new method for detecting ENR based on aptamers and AuNPs. In the absence of ENR, the aptamers attached to the surface of the AuNPs via electrostatic interactions to protect the AuNPs from NaCl, and the solution remained red. Conversely, the aptamer bonded with ENR, leading the aptamer to detach from the AuNP surface, and the color of the solution changed from red to blue. Based on this principle, ENR can be qualitatively detected by the naked eye and quantitatively detected by measuring the absorbance ratio at 650 nm and 530 nm. The experimental results showed a good linear relationship within the ENR concentration range of 0–400 nM, with a limit of detection (LOD) of 1.72 nM, which is satisfactory for detection in food safety. Additionally, this method has also been successfully applied to the detection of ENR in tap water, river water, milk, serum and urine, with good recovery rates and RSD values of less than 7%, indicating its great potential for ENR detection in environmental water samples. More importantly, the combination of this method with a smartphone platform provided great convenience for on-site and visual detection of ENR, offering promising applicability prospects.
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ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Bohan Wang,Lanhua Liu,Haopeng Zhang,Zhiqiang Wang,Kang Chen,Bo Wu,Limin Hu,Xiaohong Zhou,Lanlan Liu
Background Quinolones (QNs) widely exist in the environment due to their wide range of applications and poor metabolic properties, resulting in the generation and spread of resistance genes, posing a potential threat to human health. Traditional analytical methods cannot detect all broad ranges of QNs simultaneously. The development of facile, efficient and reliable method for quantification and assessment of the total QNs is a long-lasting challenge. Results We hereby provide a simple, sensitive and instantaneous group-targeting biosensor for the detection of total QNs in environmental water samples. The biosensor is based on a group-specific antibodies with high affinity against QNs. Fluorescent labeled antibodies bound to the coated antigen modified on the surface of the transducer, and excited by the evanescent waves. The detected fluorescent signal is inversely proportional to the QNs concentration. This biosensor exhibited excellent performance with detection limits lower than 0.15 μg L −1 for all five QNs variants, and even lower than 0.075 μg L −1 for ciprofloxacin (CIP) and ofloxacin (OFL). Environmental water samples can be detected after simple pretreatment, and all detection steps can be completed in 10 min. The transducer has a high regenerative capacity and shows no significant signal degradation after two hundred detection cycles. The recoveries of QNs in a variety of wastewater range from 105 to 119%, confirming its application potential in the measurement of total QNs in reality. Significance The biosensor can realize rapid and sensitive detection of total QNs in water samples by simple pretreatment, which overcomes the disadvantage of the traditional methods that require complex pretreatment and time-consuming, and pave the groundwork for expansive development centered around this technology.
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JOURNAL OF MOLECULAR LIQUIDS
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2017
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IF 3.648
Yuqin Wu,Hongmei Zhang,Yanqing Wang
In the present study, we have investigated the thermal stability of bovine serum albumin (BSA) in the presence of TiO 2 nanoparticles (TiO 2 NPs). The effects of TiO 2 NPs on the transportation function of BSA were also studied by fluorescence spectral method . The circular dichroism (CD) study revealed that addition of TiO 2 NPs moderately decreased the α-Helix content of the protein with the abruptly changing its tertiary structure by hydrogen bonding , van der Waals or electrostatic forces . It has been found that TiO 2 NPs acted as a structure destabilizer for BSA during the thermal denaturation process. The fluorescence quenching and molecular modeling data indicated the existence of strong interaction between BSA and the TiO 2 NPs surface that changed the binding affinities of some ligands for BSA, implying that TiO 2 NPs in blood will affect the transporting ability of serum albumin.
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JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Xuejing Ma,Han Yeong Kaw,Jing Yu,Qi Yang,Lizhong Zhu,Wei Wang
The extensive consumption of antibiotics has been reported to significantly promote the generation of antibiotic resistance (ABR), however, a quantitative causal relationship between antibiotic exposure and ABR response is absent. This study aimed to pinpoint the accurate regulatory concentration of fluoroquinolones (FQs) and to understand the biochemical mechanism of the mutual action between FQ exposure and FQ resistance response. Highly sensitive analytical methods were developed by using UPLC-MS/MS to determine the total residual, extracellular residual, total intracellular, intracellular residual and intracellular degraded concentration of three representative FQs, including ciprofloxacin (CIP), ofloxacin (OFL) and norfloxacin (NOR), with detection limits in the range of 0.002–0.057 μg/L, and recoveries in the range of 80–93%. The MICs of Escherichia coli ( E. coli ) were 7.0–31.4-fold of the respective MIC 0 after 40-day FQ exposure, and significant negative associations were discovered between the intracellular (residual, degraded or the sum) FQ concentrations and FQ resistance. Transcriptional expression and whole-genome sequencing results indicated that reduced membrane permeability and enhanced multi-drug efflux pumps contributed to the decreasing intracellular concentration. These results unveiled the pivotal role of intracellular concentration in triggering FQ resistance, providing important information to understand the dose-response relationship between FQ exposure and FQ resistance response, and ascertain the target dose metric of FQs for eliminating FQ resistance crisis.
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Journal of Water Process Engineering
·
2025
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IF 6.7
Jincheng Dai,Shuyue He,Xiyu Song,Chuliang Shen,Hongjuan Zhang,Ruyi Xie
The application of Fe-based metal-organic frameworks in photo-Fenton antibiotic degradation is limited by the low charge separation efficiency and slow Fe 3+ /Fe 2+ transition. In this study, a novel Z -scheme porous carbon nitride/mixed-valent MIL-53(Fe) (KCM-20) heterojunction photocatalyst was successfully constructed for degradation of high concentration tetracycline. On the one hand, the Z -scheme heterojunctions system is conducive to the rapid separation of photogenerated electron-hole pairs. On the other hand, the cyclic conversion of Fe 3+ /Fe 2+ in the MIL-53(Fe) photo-Fenton system can be accelerated. The KCM-20 showed the highest degradation efficiency for tetracycline. The KCM-20/light/H 2 O 2 system degraded more than 93 % of tetracycline (150 mg·L −1 ) within 75 min and exhibited the highest degradation kinetic constant, which is 8.0 and 2.4 times higher than those of carbon nitride and MIL-53(Fe), respectively. Its excellent catalytic performance can be attributed to the efficient synergy between photocatalytic reaction and Fenton reaction, producing more active species for tetracycline degradation. EPR and activity capture experiments indicate that ·O− 2, ·OH and h + are the main active species for tetracycline degradation. By analyzing the intermediate products, three degradation pathways for tetracycline were proposed. This work provides a new pathway for the preparation of efficient and cost-effective photocatalysts for the degradation of organic pollutants.
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Foods
·
2025
·Vol. 14
·期 3
·538
·
IF 5.1
Jie Wu,Yuanjie Qin,Xiaoxin Mei,Lin Cai,Wen Hao,Guozhen Fang
Ciprofloxacin (CIP), a widely used broad-spectrum antibiotic, poses a serious threat to human health and environmental safety due to its residues. The complementary monomers molecularly imprinted electrochemiluminescence sensor (MIECLS) based on a polyvinylpyrrolidone-functionalized copper nanowires (CuNWs@PVP) luminescent probe was constructed for the ultra-sensitive detection of CIP. CuNWs with low cost and high conductivity exhibited near-infrared electrochemiluminescence (NIR ECL) properties, yet their self-aggregation and oxidation led to a weakened emission phenomenon. PVP with solvent affinity and large skeleton was in situ attached to CuNWs surface to avoid CuNWs sedimentation and aggregation, and self-enhanced ECL signals were achieved. The bifunctional monomers molecularly imprinted polymer (MIP) possessed complementary active centers that increased their affinity with CIP, enhancing the accurate and sensitive detection of the target substances. The linear range of CIP using MIECLS was 5.00 × 10 −9 –5.00 × 10 −5 mol L −1 with a low limit of detection (LOD) of 2.59 × 10 −9 mol L −1 , while the recovery rates of CIP in the spiking recovery experiment were 84.39% to 92.48%. The combination of bifunctional monomer MIP and NIR copper-based nano-luminescent probe provides a new method for the detection of CIP in food.
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JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Ruixian Zhang,Wenjie Chen,Yanfei Gao,Heping Li,Yanyue Lu,Zeguang Zhou
In this study, a novel dynamic polyphenol polymerization method for the preparation of nanofiltration membrane intercalations was proposed. In this method, TA, PEI and Al 3+ could successively form the first polyphenol-amine crosslink network and the second metal-phenol cross-linked network on the surface of the support layer. The dense crosslinked layer of TA-PEI-Al 3+ formed under dynamic conditions maintains high hydrophilicity compared to the crosslinked network formed under a single pH environment. Aluminum ions possess strong electronegativity since they have relatively high valence states and small ionic radius among transition metal ions. Therefore, this is the first time to incorporate Al 3+ into phenol-amine crosslinked networks to form the metal-phenol tri-complexes. The tri-complexes of TA and Al 3+ are more robust, promoting the coordination with more water molecules and forming more stable hydrophilic intermediate layers. When an interfacial polymerization reaction is performed on the intermediate layer, this hydrophilic intermediate layer can store aqueous-phase PIP and slow down the IP reaction rate through electrostatic interactions. This process helps to form a dense PA separation layer on the support layer surface. The prepared TFC-NF membranes can be used to separate dyes, antibiotics, and compound inorganic salts. The outstanding water permeance of the TFC-NF membrane was determined as 18.7 L/m 2 ·h·bar and the removal rate of mixed pollutants can reach 99 %. The firm intermediate layer enables the optimized modified membrane to maintain stable flux and high rejection rate in 72 h separation test. This modification method provides a useful reference for the preparation of high-performance nanofiltration membranes with plant polyphenols.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Na Tian,Bin Wu,Wen-Hai Zhang,Cheng-Gang Jin,Ming-Jie Yin,Quan-Fu An
Zwitterionic nanofiltration membranes, with high rejection to organic molecules while low rejection to monovalent salts, are promising for antibiotic desalination. However, due to the heterogeneous issue of interfacial polymerization, the residual amine groups result in polymer chain aggregation via hydrogen bonds, blocking the mass transfer of water and monovalent salts. Herein, EtOH-assisted Michael-addition/Schiff-base reaction strategy was proposed to solve the issue. NaOH was initially utilized to provisionally interrupt the hydrogen bonds between polymer chains. Meanwhile, with the assistance of EtOH, gallic acid diffused into the membrane and reacted with the amine groups on the polymer chains to form covalent bonds, which thoroughly destroyed the stacking of polymer chains. As a consequence, water permeance and antibiotic desalination efficiency of the membrane are improved. For example, the pure water permeance reaches 8.9 L m −2 h −1 bar −1 , 1.7-fold enhancement compared with the pristine membrane. The membrane is applied for antibiotic desalination, offering long-term running stability, antifouling ability, as well as high efficiency in concentrated antibiotics.
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