JOURNAL OF CHEMICAL THERMODYNAMICS
·
2016
·
IF 2.196
Yi Yu,Fang Zhang,Xiaoqiang Gao,Li Xu,Guoji Liu
In this research, solid-liquid equilibrium for the ternary system (2-naphthaldehyde + o -phthalic anhydride + 1,4-dioxane) was measured at 283.15 K, 293.15 K and 303.15 K, respectively. Three isothermal phase diagrams of the system were plotted using the values of the experimental solubility. Pure 2-naphthaldehyde and o -phthalic anhydride were formed at (283.15, 293.15 and 303.15) K, and were determined by Schreinemaker’s wet residue method. The crystallization region of o -phthalic anhydride was found to be much larger than that of 2-naphthaldehyde at a certain temperature. The experimental solubility at multiple temperatures was correlated by the NRTL and Wilson models. The NRTL model was more accurate than the Wilson model in this system. The diagram of density versus component was also constructed.
研究领域:
化学
·
物理化学
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DOI
高影响因子
European Review for Medical and Pharmacological Sciences
·
2017
·Vol. 21
·期 10
·2316-2328
·
IF 1.778
An G,Zhang WB,Ma DK,Lu B,Wei GJ,Guang Y,Ru CH,Wang YS
Objective To investigate the influence of VEGF/BMP-2 on the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells BMSCs) on PLGA/gelatin composite scaffold. Materials and methods Randomly-oriented nanofibers with different ratios of Poly Lactic-co-Glycolic Acid (PLGA)/gelatin were produced through electrospinning. The mixture of nanofibers and BMSCs was pipetted onto the surface of the scaffolds, and BMSCs/PLGA/gelatin composite was obtained. The surface morphology, chemical structure, hydrophilicity and mechanical property of PLGA/gelatin nanofibers were revealed by scanning electron microscope. In vitro release kinetics of bone morphogenetic protein (BMP-2) and vascular endothelial growth factor (VEGF) were studied using ELISA kits. The cell adhesion, growth and proliferation of BMSCs on scaffolds were observed by scanning electron microscopy. The CCK-8 assay was used to evaluate the effects of VEGF/BMP-2 slow release system on the proliferation of BMSCs on scaffolds. RT-PCR was used to examine the activities of alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX-2), and osteocalcin (OCN). Results In each group of cells in the in-vitro experiment, through electron microscope scanning, fiber scaffolds were interconnected three-dimensional reticular structure, BMSCs firmly attached to the fiber surface and internal stent, cells experienced a long spindle, polygon change, and branch-like protrusions on the cell surface were connected. Under the electron microscope, cell proliferation curve and osteogenesis markers (ALP, RUNX-2, OCN) expression in the dual factor group on cell adhesion, proliferation and differentiation were much better than those of blank control group and single factor groups. Conclusions In the successfully constructed gelatin/PLGA nanofiber scaffold, VEGF and BMP-2 can be sequentially released, during which VEGF and BMP-2 can promote the adhesion, proliferation, and differentiation of BMSCs.
研究领域:
生物医学工程
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组织工程
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细胞生物学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 46
·期 5
·2259-2265
·
IF 3.591
Yanting Xu,Ke-Jian Jiang,Pengcheng Wang,Wei-Min Gu,Guang-Hui Yu,Xueqin Zhou,Yanlin Song
3D tin-based halide perovskites are promising light absorbers for perovskite solar cells (PSCs) due to their non-toxicity, suitable optical band gaps, and excellent optoelectronic properties. However, the inherent rapid crystallization and easy oxidation of Sn2+ to Sn4+ in 3D perovskites result in a low power conversion efficiency (PCE) and poor stability of tin perovskite solar cells compared with the lead counterparts. In this report, quasi-2D hybrid tin-based perovskite films are fabricated by using 2-thiopheneethylammonium (TEA+) as the spacer cation in MASnI3 (MA: CH3NH3+), leading to the formation of quasi-2D perovskite films with high orientation and a low content of Sn4+. As a result, the perovskite solar cells show a considerable power-conversion efficiency of 6.8%, which is among the highest values reported for MASnI3-based solar cells. Moreover, the devices exhibited excellent stability compared with the device without TEAI.
研究领域:
材料科学
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能源技术
·
光伏器件
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DOI
高影响因子
CORROSION SCIENCE
·
2020
·
IF 6.479
Shan Wan,Xiao-Ze Ma,Chun-Hui Miao,Xin-Xin Zhang,Ze-Hua Dong
Initial atmospheric corrosion of clean copper and NaCl-deposited copper was investigated by quartz crystal microbalance and electrochemical impedance spectroscopy. 2-phenyl imidazoline (2-PI) exhibits increasing inhibition efficiency with temperature on chloride-induced atmospheric corrosion of Cu. However, on the NaCl-deposited Cu, adsorption amount of 2-PI is much higher than that on clean Cu possibly due to that rough corrosion products entrap lots of 2-PI molecules. The adsorption of 2-PI on corroded Cu is suggested to obey a capillary condensation and entrapment mechanism, but in dry argon atmosphere, it obeys a simple pseudo first-order model for lack of corrosion products.
研究领域:
材料科学
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腐蚀与防护
·
电化学
✓ 已验证
DOI
高影响因子
DIABETES OBESITY & METABOLISM
·
2024
·
IF 5.4
Ting Zheng,Ruhao Yang,Xin Li,Zhe Dai,Hongyu Xiang
Background Diabetic nephropathy (DN) is one of the main causes of end-stage renal disease (ESRD), but its mechanism has not been clearly studied. We utilized integrative transcriptome analysis to explore the pathogenesis of DN. Methods We conducted an analysis by combining bulk dataset and single-cell transcriptome dataset. Through this approach, we identified that Serpine2 may regulate the ‘collagen-containing extracellular matrix’ pathway involved in DN. Subsequently, we established DN animal and cell models using db/db mice and mesangial cells (MCs) to validate the role of Serpine2 in DN. In the animal model, we detected the expression level of Serpine2 in DN using western blotting (WB) and immunofluorescence (IF) assays. To further clarify the molecular mechanism of Serpine2 in DN, we knocked down Serpine2 and observed its effects on MCs proliferation and extracellular matrix (ECM) accumulation. Results Our single-cell analysis of DN models highlighted a pivotal role for MCs in the disease's initiation. Next, through Cytoscape analysis of differentially expressed genes (DEGs) in MCs, we identified the following 10 hub genes: Acta2, Angpt2, Ccn1, Col4a1, Col4a2, Col8a1, Kdr, Thbs1, Tpm4 and Serpine2. Subsequently, we identified that Serpine2 and Kdr were also significantly DEGs in the bulk analysis of glomeruli. Additionally, our integrated gene set enrichment analysis of bulk dataset and single-cell RNA dataset revealed that the ‘collagen-containing extracellular matrix’ was a key pathway in DN progression. Serpine2 was one of the crucial genes involved in regulating this pathway. Therefore, we speculated that the regulation of the ‘collagen-containing extracellular matrix’ pathway by Serpine2 was an important mechanism. Importantly, WB and IF staining confirmed that Serpine2 expression was upregulated in the MCs of diabetic mice. Knockdown of Serpine2 in cultured MCs alleviated high-glucose-induced excessive MCs proliferation and ECM accumulation. Finally, we found that ERK agonist Ro 67-7476 eliminated the effect of Serpine2 siRNA. Conclusions In summary, Serpine2 regulates MCs proliferation and ECM synthesis through activation of the ERK1/2 pathway, which is an important pathogenesis mechanism of DN. These findings offer fresh perspectives on the mechanisms of glomerulosclerosis in DN pathogenesis and may provide new targets for treating DN.
研究领域:
分子生物学
·
肾脏病学
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DOI
高影响因子
GREEN CHEMISTRY
·
2024
·Vol. 26
·期 20
·10383-10389
·
IF 9.3
Haibo Zhu,Ting Zhong,Liu Yang,Yajing Shen,Qiangwen Fan,Zhanggao Le,Zongbo Xie
The photocatalytic activation of oxygen molecules (O2) is an environmentally friendly and sustainable alternative to substituted benzofuran-3(2H)-one synthesis. Herein, lead-free Cs2AgBiBr6 nanocrystals were used to activate O2 efficiently and selectively for the photocatalytic transformation of 4-hydroxycoumarins to functional 2-hydroxy-benzofuran-3(2H)-ones under an air atmosphere. Polyfunctionalized benzofuran-3(2H)-ones with a quaternary carbon center can be selectively obtained in moderate to good yields at room temperature via one-pot synthesis. Perovskite crystal structures have significant influence on photocatalytic activity due to the different charge separation abilities. The reaction mechanism was further investigated to confirm the contribution of reactive oxygen species (ROS).
研究领域:
材料化学
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有机合成
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光催化
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绿色化学
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纳米材料
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环境科学
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可持续化学
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DOI
高影响因子
Science China-Materials
·
2024
·
IF 6.8
Wei Yi,Song Menglin,Li Lihua,Ma Yingjin,Lao Xinyue,Liu Yuan,Li Guogang,Hao Jianhua
Persistent luminescence nanomaterials can remain luminescence when the light source is turned off, which exhibits promise in biosensor and bioimaging fields since they have the ability to completely eradicate tissue autofluorescence. Although significant progress has been made in the persistent luminescence biosensing, there is still a dearth of long-afterglow detection platform with low limit of detection (LOD) and high sensitivity. Herein, Zn 2 GeO 4 :Mn, Cr persistently luminescent nanorods (PLNRs) with superior persistent luminescence and long afterglow time were developed. The addition of Cr 3+ manifestly improves persistent luminescence intensity and afterglow duration through creating a deep defect trap. Then the biosensors were constructed by combining the Zn 2 GeO 4 :Mn,Cr PLNRs-antibody and Fe 3 O 4 magnetic nanoparticles (MNPs)-antibody for nucleocapsid protein detection based on electrostatic attraction. The LOD value for nucleocapsid protein realizes as low as 39.82 ag/mL, which is much lower than the previously reported persistent luminescent-based biosensors. Accordingly, the low detection sensitivity is attributed to fluorescence resonance energy transfer. In addition, high specificity is also achieved. Therefore, the as-prepared Zn 2 GeO 4 :Mn,Cr persistently luminescent materials can act as the promising candidate in biosensors applications. This strategy provides effective guidance for the development of biosensing platforms with high sensitivity and specificity.
研究领域:
材料科学
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纳米技术
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生物传感
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生物医学工程
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DOI
高影响因子
MOLECULAR DIVERSITY
·
2019
·Vol. 24
·期 2
·423-435
·
IF 2.032
Zheng Ran,Feng Fan,Zhang Zhihui,Fu Jiaxu,Su Qing,Zhang Yumin,Gu Qiang
Abstract An efficient strategy for synthesizing of 3-substituted bis-isoxazole ether bearing 2-chloro-3-pyridyl under microwave radiation was reported. The reactive regioselectivity was improved by changing mainly the solvent and acid-binding agent. 3-(2-Chloropyridin-3-yl)-5-(((3-substituted phenyl isoxazol-5-yl)methoxy)methyl)isoxazoles were synthesized in 31–92% yields and were characterized by FT-IR, HRMS, 1 H and 13 C NMR spectroscopy. The single crystal of 3-(2-chloropyridin-3-yl)-5-(((3-(p-tolyl)isoxazol-5-yl)methoxy)methyl)isoxazole was obtained, and the structure of compound has also been determined by X-ray diffraction technique. Weak intra- and intermolecular C–H∙∙∙O interactions and a C–H∙∙∙π interaction link molecules into a three-dimensional network. The results showed that the synthesized compounds belonged to triclinic system, and their regioselectivity depended on the solvent and acid-binding agent. The merits of this method include the environmentally friendly, efficient, simple operation, and higher regional selectivity. Graphic abstract An efficient synthesis of 3-substituted bis-isoxazole ethers was developed via 1,3-dipolar cycloaddition reaction starting from 3-substituted phenyl-5-((prop-2-yn-1-yloxy))methyl)isoxazoles and ( Z )-2-chloro- N -hydroxynicotinimidoyl chloride using NaHCO3 as an acid-binding agent in THF solvent-dissolved trace water under catalyst-free microwave-assisted conditions.
研究领域:
有机化学
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药物化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 9
·期 5
·1336-1350
·
IF 5.344
Jiatao Yang,Qiuyi Li,Rui Zhou,Minglu Zhou,Xi Lin,Yucheng Xiang,Dandan Xie,Yuan Huang,Zhou Zhou
Distant organ metastasis is the main cause of death in breast cancer patients. Evidences have shown that mitochondria also play a crucial role in tumor metastasis, except for as apoptosis center. However, the treatment of tumor growth and metastasis was reported to be limited by mitochondria-associated protein Bcl-2, which are gatekeepers of apoptosis and are found to reside in mitochondria mainly. Herein, we designed a mitochondria-targeting doxorubicin delivery system as well as a mitochondrial distributed Bcl-2 function-converting peptide NuBCP-9 delivery system, which are both based on N-(2-hydroxypropyl)methacrylamide copolymers, to achieve a synergistic effect on tumor regression and metastasis inhibition by combination therapy. After mitochondria were damaged by mitochondria-targeting peptide-modified doxorubicin, apoptosis was effectively enhanced by mitochondrial specifically distributed NuBCP-9 peptides, which converted Bcl-2 function from anti-apoptotic to pro-apoptotic and paved the way for the development of mitochondrial impairment. The combination treatment exhibited significant damage to mitochondria, including excess reactive oxygen species (ROS), the permeabilization of mitochondrial outer membrane (MOMP), and apoptosis initiation on 4T1 breast cancer cells. Meanwhile, besides enhanced tumor growth suppression, the combination treatment also improved the inhibition of 4T1 breast cancer metastasis both in vitro and in vivo. By increasing the expression of cytochrome C and decreasing the expression of Bcl-2, metal matrix protease-9 (MMP-9) as well as vascular endothelial growth factor (VEGF), the combination treatment successfully decreased 84% lung metastasis. Overall, our work provided a promising strategy for metastatic cancer treatment through mitochondria-targeting anti-cancer drug delivery and combination with mitochondrial distributed Bcl-2 function-converting peptide.
研究领域:
肿瘤学
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药理学
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细胞生物学
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癌症治疗
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DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2016
·
IF 3.014
Yan Yuan,Junxiang Wang,Zongqian Hu,Haiping Lei,Donghua Tian,Shuqiang Jiao
A Prussian blue analogue, Na 2 Co 3 [Fe(CN) 6 ] 2 , has been fabricated by a facile co-precipitation method at room temperature as a potential cathode material for both lithium-ion and sodium-ion batteries. It found that the Na 2 Co 3 [Fe(CN) 6 ] 2 nanoparticles with a primary particle size of 30–40 nm have been synthesized, and exhibited an excellent electrochemical performance , including a long cycling behavior in lithium-ion batteries and a good rate performance in sodium-ion batteries. In lithium-ion batteries, the Na 2 Co 3 [Fe(CN) 6 ] 2 nanoparticles cathode can deliver an excellent cyclic performance with 73.0% retention of initial capacity after 50 cycles at 50 mA g −1 . Moreover, when Na 2 Co 3 [Fe(CN) 6 ] 2 is used as cathode material of sodium-ion batteries, it exhibits an outstanding rate performance exceeding 75.2% retention of initial capacity after 50 cycles at 200 mA g −1 . Therefore, the Na 2 Co 3 [Fe(CN) 6 ] 2 nanoparticles can be a potential cathode candidate for the application of lithium-ion and sodium-ion batteries due to its excellent electrochemical performance, easy preparation, low cost and environment benefits.
研究领域:
材料科学
·
电化学
·
能源存储
·
无机化学
·
纳米材料
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yifan Chen,Fan Yang,Xiaomin Hou
As representative compounds spanning three generations of diamide insecticides, flubendiamide, chlorantraniliprole, and tetraniliprole have been extensively employed in agricultural practices. However, their widespread application induces oxidative stress in plants and underlying toxic mechanisms remain poorly understood. Given the important roles in oxidative stress, glutathione reductase 2 from Arabidopsis thaliana ( At GR2) was applied as a biomarker to evaluate the toxicological effects of diamide insecticides. Firstly, the root elongation in Arabidopsis seedlings was significantly inhibited and the At GR2 gene expression level was up-regulated under exposure of the diamide insecticides. Then the glutathione reductase activity of At GR2 was decreased with the increase concentration of these three insecticides, especially for flubendiamide with similar detrimental effects at much lower concentrations. Moreover, the binding affinity between flubendiamide and At GR2 was also stronger than that of chlorantraniliprole and tetraniliprole, which was conformed with molecular docking results. Additionally, much more evident effects on the structure of At GR2 under exposure of flubendiamide was detected through multispectral techniques compared to the other two insecticides. Hence this study systematically explored the binding mechanism of these three-generations of diamide insecticides with At GR2 at the molecular level, and further provided critical assessments of their phytotoxic effects on plant development and agricultural systems.
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产品关联
DOI
PubMed
高影响因子
Metabolites
·
2022
·Vol. 12
·期 7
·610
·
IF 5.581
Lin-Lin Cao,Yi Han,Lin Pei,Zhi-Hong Yue,Bo-Yu Liu,Jing-Wen Cui,Mei Jia,Hui Wang
Type 2 diabetes mellitus (T2DM) has been identified as an independent risk factor for hepatocellular cancer (HCC). However, there are no ideal biomarkers for the surveillance and early detection of HCC in the T2DM population at present. In this study, we aimed to explore novel metabolite biomarkers for T2DM-positive [T2DM(+)] HCC by metabolomic analysis. At first, many serum metabolites were found dysregulated in T2DM(+) HCC patients in untargeted metabolomic analyses. Targeted metabolite analyses confirmed that serum benzoic acid and citrulline were increased, and creatine was decreased in T2DM(+) HCC compared to the T2DM group. A metabolite classifier including benzoic acid, creatine, and citrulline was identified as a novel biomarker for the diagnosis of T2DM(+) HCC, with an area under the ROC curve (AUC) of 0.93 for discriminating T2DM(+) HCC patients from T2DM patients. In addition, the metabolite classifier detected small-size (AUC = 0.94), early-stage (AUC = 0.94), and AFP-negative (AUC = 0.96) tumors with high sensitivity and specificity. The combination of this metabolite classifier and AFP might be useful in the surveillance and early detection of HCC in the T2DM population. In conclusion, this study establishes a novel diagnostic tool for T2DM(+) HCC.Keywords:hepatocellular cancer;type 2 diabetes mellitus;metabolomics;metabolite classifier;benzoic acid;creatine;citrulline
研究领域:
肿瘤学
·
糖尿病学
·
代谢组学
·
生物标志物研究
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）