BIOMATERIALS
·
2021
·
IF 12.479
Jiankun Guan,Yuxin Wu,Xin Liu,Huimin Wang,Ningbing Ye,Zheng Li,Chen Xiao,Zhijie Zhang,Zifu Li,Xiangliang Yang
Cancer stem cells (CSCs) present grand challenges for triple-negative breast cancer (TNBC). Conventional chemotherapy drugs, including Camptothecin (CPT), not only cannot eradicate CSCs but also foster a suppressive immune microenvironment for the initiation and proliferation of CSCs. Herein, we report a novel prodrug CPT–SS–NLG919 (CN) and its nanoformulation [email protected] ( [email protected] ), which potently suppress CSCs by regulating CSCs niche in murine TNBC 4T1 tumors. Via inducing immunogenic cell death (ICD) and simultaneous inhibiting indoleamine 2, 3-dioxygenase (IDO), CN and [email protected] promote DC maturation, decrease Treg cells, mitigate tryptophan consumption, and reduce the amount of IL-6, IL-13, and TGF-β, converting CSCs niche to a hostile condition for CSCs to live in and eliminating CSCs efficiently, thereby inducing efficient tumor inhibition in 4T1 tumor models. Our work represents a new paradigm of eliminating CSCs by regulating tumor immune microenvironment and suggests that CN and its nanoformulation [email protected] are promising candidates for the treatment of intractable TNBC.
研究领域:
肿瘤学
·
药理学
·
免疫学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 190
·期 3
·826-838
·
IF 2.14
Liu Hang,Zhou Min,Ju Xin,Shu Hang,Hu Cuiying,Li Liangzhi
β-Glucosidase (BG) hydrolyzes cellobiose into glucose, and is a vital step in converting ionic liquids (ILs)-pretreated biomass to sustainable biofuels. The inactivation mechanism of BG from Paenibacillus sp. LLZ1 induced by microcrystalline cellulose was explored in various concentrations of ILs, composed of [Emim] + cation and [DEP] − , [OAc] − , [Br] − , [Cl] − , and [BF 4 ] − anions. The FTIR analysis of inactivated BG indicated that the ILs altered its β-sheet content. Moreover, circular dichroism spectroscopy (CD) suggested that the α-helix content decreased, while the β-sheet content increased with the presence of ILs in general. Interestingly, the secondary structure of BG had almost no change after [Emim]DEP treatment, while ionic liquid [Emim]BF 4 treatment caused the irreversible denaturation of BG. Eventually, by adding 0.4 mM of Aerosol OT surfactant, the BG activity was increased by 20.1% in the presence of 25% [Emim]DEP, and the corresponding glucose yield from hydrolysis of cellobiose was increased by 23.9%.
研究领域:
生物化学
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生物技术
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酶学
·
可持续能源
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DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2024
·
IF 6.1
Yongquan Zhang,Hongchang Gao,Jingshun Wang,Qingguo Chi,Tiandong Zhang,Changhai Zhang,Yu Feng,Yue Zhang,Dianxue Cao,Kai Zhu
All-solid-state lithium metal batteries (ASSLMBs) are considered the most competitive next-generation energy storage batteries due to their high energy density and high safety. Polyethylene oxide (PEO) is an ideal solid-state electrolyte due to its high chain flexibility, high dielectric constant, and strong dissociation ability towards lithium salts. However, the application of PEO is severely hindered by drawbacks such as low ion conductivity at room temperature and low electrochemical window. In this work, we constructed a Li1.25Al0.25Zr1.75(PO4)3 (LAZP)/PEO composite solid-state electrolyte (CSSE) with improved ion conductivity, enlarged electrochemical window, and stable charge–discharge cycle performance. The CSSE leads to an obvious LiF layer and Li2CO3 formation at the interface of lithium metal. Meanwhile, the addition of LAZP reduces the side reaction between PEO and the lithium metal significantly and suppresses the deterioration of ion conductivity. In addition, we modified LiFePO4 (M-LFP), the cathode sheet of the ASSLMBs, by adding an appropriate proportion of multi-walled carbon nanotubes (MWCNTs), PEO and lithium bis(trifluoromethane sulfonimide) (LiTFSI) to improve the ionic and electronic conductivity inside the cathode of the ASSLMBs. The Li/CSSE/M-LFP full cell exhibits a specific discharge capacity of 152 mA h g−1 at 60 °C and 2 C rate and a capacity retention rate of 93.5% after 200 cycles at 1 C. This work demonstrates an effective strategy for increasing the specific capacity, rate ability and long-cycle performance of ASSLMBs.
研究领域:
材料科学
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电化学
·
能源存储
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Wen Chen,Xiaodong Zheng,Fujie Yan,Lizhou Xu,Xiang Ye
Microplastics derived from plastic waste have emerged as a pervasive environmental pollutant with potential transfer and accumulation through the food chain, thus posing risks to both ecosystems and human health. The gut microbiota, tightly intertwined with metabolic processes, exert substantial influences on host physiology by utilizing dietary compounds and generating bacterial metabolites such as tryptophan and bile acid. Our previous studies have demonstrated that exposure to microplastic polystyrene (PS) disrupts the gut microbiota and induces colonic inflammation. Meanwhile, intervention with cyanidin-3-O-glucoside (C3G), a natural anthocyanin derived from red bayberry, could mitigate colonic inflammation by reshaping the gut bacterial composition. Despite these findings, the specific influence of gut bacteria and their metabolites on alleviating colonic inflammation through C3G intervention remains incompletely elucidated. Therefore, employing a C57BL/6 mouse model, this study aims to investigate the mechanisms underlying how C3G modulates gut bacteria and their metabolites to alleviate colonic inflammation. Notably, our findings demonstrated the efficacy of C3G in reversing the elevated levels of pro-inflammatory cytokines (IL-6, IL-1β, and TNF-α) and the upregulation of mRNA expression (Il-6, Il-1β, and Tnf-α) induced by PS exposure. Meanwhile, C3G effectively inhibited the reduction in levels (IL-22, IL-10, and IL-4) and the downregulation of mRNA expression (Il-22, Il-10, and Il-4) of anti-inflammatory cytokines induced by PS exposure. Moreover, PS-induced phosphorylation of the transcription factor NF-κB in the nucleus, as well as the increased level of protein expression of iNOS and COX-2 in the colon, were inhibited by C3G. Metabolisms of gut bacterial tryptophan and bile acids have been extensively implicated in the regulation of inflammatory processes. The 16S rRNA high-throughput sequencing disclosed that PS treatment significantly increased the abundance of pro-inflammatory bacteria (Desulfovibrio, norank_f_Oscillospiraceae, Helicobacter, and Lachnoclostridium) while decreasing the abundance of anti-inflammatory bacteria (Dubosiella, Akkermansia, and Alistipes). Intriguingly, C3G intervention reversed these pro-inflammatory changes in bacterial abundances and augmented the enrichment of bacterial genes involved in tryptophan and bile acid metabolism pathways. Furthermore, untargeted metabolomic analysis revealed the notable upregulation of metabolites associated with tryptophan metabolism (shikimate, l-tryptophan, indole-3-lactic acid, and N-acetylserotonin) and bile acid metabolism (3b-hydroxy-5-cholenoic acid, chenodeoxycholate, taurine, and lithocholic acid) following C3G administration. Collectively, these findings shed new light on the protective effects of dietary C3G against PS exposure and underscore the involvement of specific gut bacterial metabolites in the amelioration of colonic inflammation.
研究领域:
环境科学
·
微生物学
·
免疫学
·
营养科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Hong-Ru Zhao,Bing-Mei Su,Yi-Bing Shi,Juan Lin
Ethyl 3-hydroxy-3-phenylpropionate (EHPP), ( R )-EHPP or ( S )-EHPP, is an important chiral intermediate for pharmaceuticals. Its synthesis from ethyl benzoyl acetate (EBA) by alcohol dehydrogenase is regarded as a green method. However, scarcely any alcohol dehydrogenase has been reported competent in asymmetric synthesis of chiral EHPP at high EBA loading. Present study developed two robust and efficient bio-catalysts Mu-S2 and Mu-R4 for preparation of ( S )-EHPP and ( R )-EHPP respectively by rational design of alcohol dehydrogenase PcSDR from Pedobacter chitinilyticus based on molecular dynamics (MD) simulation analysis. BtGDH, a glucose dehydrogenase from Bacillus toyonensis catalyzing the oxidation of glucose for cofactor regeneration, was co-expressed with the screened mutants to form enzyme systems Mu-S2-BtGDH and Mu-R4-BtGDH. After reaction condition optimization, Mu-S2-BtGDH and Mu-R4-BtGDH were efficient in the synthesis of ( S )-EHPP (94% conv. and 99% e.e.) and ( R )-EHPP (99% conv. and 98% e.e. ) respectively in 100 mL scale under 500 mM of EBA loading in 10 h following a substrate continuous feeding mode. After purifying, the isolated yield for each EHPP enantiomer is > 93%. This work not only provides potential biocatalysts for the industrial production of ( R )-EHPP and ( S )-EHPP, but also enriches the constructure-function relationship of alcohol dehydrogenases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CRYSTAL GROWTH & DESIGN
·
2021
·
IF 4.076
Yunqiao Guo,Die Zhang,Ting Zheng,Ling Huang,Daojiang Gao,Jian Bi,Guohong Zou
Two new alkali metal formic-borates named Rb3(COOH)3(H3BO3)2 and Cs3(COOH)3(H3BO3)2 have been successfully synthesized under mild conditions. Influenced by the different cation sizes, these two stoichiometrically equivalent compounds reveal discrepant symmetries: Rb3(COOH)3(H3BO3)2 is noncentrosymmetric (NCS) while Cs3(COOH)3(H3BO3)2 is centrosymmetric (CS). Especially, these two title compounds exhibit unique planar [(COOH)3(H3BO3)2]3– layers constructed from two kinds of planar π-conjugated asymmetric BO3 and COOH units, which induce the large birefringence, 0.09@1064 nm for Rb3(COOH)3(H3BO3)2 and 0.10@1064 nm for Cs3(COOH)3(H3BO3)2, respectively. Optical property measurements indicate that these two compounds display short UV absorption edges, suggesting that they are promising short-wave UV birefringent materials. A detailed theoretical analysis confirmed that the excellent linear optical property was derived from the synergistic effect of the two kinds of π-conjugated planar groups.
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产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 2
·361
·
IF 3.06
Haili Wang,Wenfeng Han,Xiliang Li,Bing Liu,Haodong Tang,Ying Li
Cr2O3nanoparticles were prepared by solution combustion synthesis (SCS) with chromium nitrate as the precursor and glycine as the fuel. Commercial Cr2O3and Cr2O3prepared by a precipitation method were also included for comparison. The morphology, structure, acidity and particle size of fresh and spent Cr2O3catalysts were investigated by techniques such as XRD, SEM, TEM, BET and NH3-TPD. In addition, catalytic performance was evaluated for the dehydrofluorination of 1,1,1,3,3-pentafluoropropane (CF3CH2CHF2, HFC-245fa) to 1,3,3,3-tetra-fluoropropene (CF3CH=CHF, HFO-1234ze). The catalytic reaction rate of Cr2O3prepared by SCS method is as high as 6 mmol/h/g, which is about 1.5 times and 2 times higher than that of precipitated Cr2O3and commercial Cr2O3, respectively. The selectivity to HFO-1234ze for all the catalysts maintains at about 80%. Compared with commercial and precipitated Cr2O3, Cr2O3-SCS prepared by SCS possesses higher specific surface area and acid amount. Furthermore, significant change in the crystal size of Cr2O3prepared by SCS after reaction was not detected, indicating high resistance to sintering.
研究领域:
材料化学
·
催化化学
·
纳米材料科学
·
环境友好化工
·
氟化学
✓ 已验证
DOI
高影响因子
Materials Research-Ibero-american Journal of Materials
·
2019
·
IF 1.104
Dan Ho Kim,Tap Tran Duy,Nguyen-Truong Hieu,Ty Nguyen Minh,Zhou Dacheng,Qiu Jianbei
The SiO2-Al2O3-BaF2-TiO2-CaF2 transparent silicate glass-ceramics containing BaF2 nanocrystals were successfully prepared by heat treatment process through conventional melting method. Effects of heat treatment processes and Yb3+ concentration on the downconversion (DC) emission of the co-doped Er3+/Yb3+ transparent silicate glass-ceramics were investigated. With the increase of temperatures and times of heat treatment process, the DC emission intensity of the co-doped Er3+/Yb3+ glass-ceramics was significantly enhanced. At the same time, with the increase of Yb3+ concentration, the value of DC intensity of Er3+/Yb3+ co-doped bands centered at 849, 883 and 1533 nm is maximized when the concentration of Yb3+ reaches 2.5 mol.%. When the concentration exceed 2.5 mol. %, the DC emission intensity of Er3+/Yb3+ co-doped bands centered at 849, 883 and 1533 nm was decreased, owing to the self-quenching effect. It’s interesting that the DC emission intensity of Er3+/Yb3+ co-doped band centered at 978 nm didn’t quench when the Yb3+ concentration exceed 2.5 mol. %. At the same time, the DC mechanism and ET processes between Yb3+ and Er3+ ions were discussed.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Dai-Xu Wei,Duanfang Cai,Youguo Tan,Kezhi Liu,Jin-Wei Dao,Xiang Li,Aikeremujiang Muheremu
Huperzine A (HupA) is used in Alzheimer's disease (AD) therapy for its effective inhibition of acetylcholinesterase (AChE) and enhancement of cholinergic neuronal function. However, direct oral administration and injection of HupA cause side effects like nausea, anorexia, and rapid metabolism. Using a tripolymer, poly(3-hydroxybutyrate- co -3-hydroxyvalerate-co-3-hydroxyhexanoate (PBVHx), from the polyhydroxyalkanoate (PHA) family synthesized via synthetic biology, we present a novel AD therapy strategy with peritoneally administered PBVHx microspheres loaded with HupA (HupA-PBVHxMs). This approach extends HupA's metabolic duration in the blood and brain, enhancing AChE inhibition efficacy. Uniformly sized HupA-PBVHxMs, created using microfluidics and rotary evaporation, show up to 70.4 % drug encapsulation efficiency, sustained HupA release for 40 days, reduced neurotoxicity from Aβ25–35, and maintained in vivo HupA supply and AChE inhibition for over 20 days. In cognitive tests, HupA-PBVHxMs improved function in AD mice. Thus, PBVHx microspheres with slower HupA release and lower biotoxicity offer a superior platform for sustained AChE inhibitor release, outperforming commercial PLGA microspheres.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
KEY ENGINEERING MATERIALS
·
2013
·
IF 0
Yun Xia Jiang,Hu Jin Zuo,Yong Jia Shen,Feng Ping Yi
The Friedel-Crafts acylation reaction between 5-cyanoindole and 4-chlorobutyryl chloride was conducted in ionic liquid 1-buty-3-methylimidazolium chloroaluminate ([bmiCl/AlCl3), the major product was 5-cyano-3-(4-chlorobuty) indole. The structure of the product was characterized by Mass and 1H NMR spectra. When the molar ratio of AlCl3 to [bmiCl in [bmiCl/AlCl3 is at 1:1 or less, no 5-cyano-3-(4-chlorobuty) indole could be obtained. The suitable molar ratios of AlCl3 to [bmiCl in [bmiCl/AlCl3 were from 2:1 to 3:1, the yields of the major product were from 68% to 70%, respectively. When the molar ratio of [bmiCl/AlCl3 to 5-cyanoindole was less than 2:1, the acylation reaction was difficult to carry out, because 5-cyanoindole would be not totally dissolve in [bmiCl/AlCl3 under the situation.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
MATERIALS CHEMISTRY AND PHYSICS
·
2018
·
IF 2.21
Jinyuan Bai,Yang Liu,Yanjun Hou,Shuhong Wang
A new luminescent europium(III) bis-β-diketonate complex, Eu 2 (PBT) 3 (NO 3 ) 3 (PBT = 1,1-(pyridine-2,6-diyl)bis(4,4,4-trifluoro-1,3-dione), was incorporated into the poly(methylmethacrylate) (PMMA) matrix and formed nanofibers via electrospinning technology. The different characterization techniques were adopted to reveal the effect of Eu 2 (PBT) 3 (NO 3 ) 3 on the spatial structure and optical properties of the nano-composite fibers obtained. The doping enhances the thermal stability and filament-forming properties for a series of PMMA-supported composite nanofibers Eu/PMMA, and in particular, the doping concentrations of 11 wt% also showed more efficient luminescence properties. Furthermore, the Judd-Ofelt theory was applied in this study to explain the effect of the distribution of Eu 2 (PBT) 3 (NO 3 ) 3 and the mutual effect of the Eu 2 (PBT) 3 (NO 3 ) 3 coordination compound and surrounding fractions of PMMA.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
SYNTHESIS-STUTTGART
·
2025
·Vol. 57
·期 14
·2241-2251
·
IF 2.3
Yilun Song,Deyang Liu,Shunxi Dong,Xiaoming Feng
A highly enantioselective alkoxylation reaction of 3-bromo-3-substituted 2-oxindoles with alcohols was achieved by a chiral Ni(II)/N,N′-dioxide complex. This strategy provided an efficient route to various enantioenriched 3-alkyl 3-alkyloxy-2-oxindoles with good yield and enantiomeric ratio (40 examples, up to 99% yield, 95:5 er). Moreover, phenol and benzenethiol were also compatible with the reaction conditions. The gram-scale synthesis of an optically active product and its further transformation into CPC-1 analogue demonstrated the potential utility of the method. In addition, a catalytic cycle as well as a possible working mode was provided to understand the enantioselectivity of the reaction.
研究领域:
有机化学
·
不对称催化
·
药物化学
·
合成方法学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）