Journal of Water Process Engineering
·
2023
·
IF 7
Zijun Xie,Bo Sun,Hongying Lin,Meng Fu,Yi Zhang,Zequn Ma,Xiangming Li
Dephosphorylation that separates phosphate groups from substrates is a significant reaction for both living organisms and environmental protection. Meanwhile, 4-nitrophenol (4-NP) is known to be a popular organic compound used for pesticides production, which is genotoxic and carcinogenic to humans and animals. Herein, we have developed a bifunctional catalyst that can be used for simultaneous dephosphorylation and 4-nitrophenol (4-NP) reduction when para-nitrophenyl phosphate (p-NPP) serves as the model compound. This bifunctional catalyst was obtained by in-situ growth of NiFe layered double hydroxide on the reduced graphene oxide/polyelectrolytes assembly nanolayers (denoted as rGP 3.5 /NiFe LDH). The results demonstrate that the rGP 3.5 /NiFe LDH can activate dephosphorylation of p-NPP at 20 °C and the catalytic efficiency increases significantly with increasing temperature in the range of 20 °C to 90 °C. Moreover, by-product 4-NP formed through p-NPP dephosphorylation is further fully converted to 4-aminophenol by the rGP 3.5 /NiFe LDH within 15 min. As far as we know, this is the first example for both dephosphorylation and 4-NP reduction through NiFe LDH as bifunctional catalysts, which paves the way for the development of multiple potential applications for NiFe LDH.
研究领域:
催化
·
环境化学
·
材料科学
·
纳米技术
✓ 已验证
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2021
·
IF 5.275
Lian Xu,Li-Chao Wang,Bing-Mei Su,Xin-Qi Xu,Juan Lin
(2 S , 3 R )-4-methylsulfonylphenylserine [(2 S , 3 R )-MPS], a key chiral precursor for antibiotics florfenicol and thiamphenicol, could be asymmetrically synthesized by l -threonine transaldolase (LTTA) coupled with an acetaldehyde elimination system. The low efficiency of acetaldehyde elimination system blocked further accumulation of (2 S , 3 R )-MPS. To address this issue, strengthening acetaldehyde elimination system and enzyme self-assembly strategy were combined to accelerate biosynthesis of (2 S , 3 R )-MPS. The new multi-enzyme cascade with intensified acetaldehyde elimination system BL21 (PsLTTAD2/ScADH/BtGDH) could produce (2 S , 3 R )-MPS with a titer of 157.6 mM, 1.7-folds than that produced by the original system BL21 (PsLTTAD2/ApADH/CbFDH). Moreover, self-assembly of PsLTTAD2 and ScADH by respective fusion of SpyTag and SpyCatcher were carried out to develop a self-assembled multi-enzyme cascade BL21 (ST-PsLTTAD2/SC-ScADH/BtGDH). As a result, the yield of (2 S , 3 R )-MPS was up to 248.1 mM with 95% de . As far as we knew, that represented the highest yield of (2 S , 3 R )-MPS by enzymatic synthesis, and therefore was a promising and green route for industrial production of this valuable compound.
研究领域:
生物催化
·
酶工程
·
生物技术
·
化学生物学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Wenting Lan,Changgan Lai,Yasheng Dai,Shijiao Fu,Chenjie Gu,Tao Jiang,Yuning Pan
The early and reliable detection of prostate cancer remains a significant challenge in clinical diagnostics. This study presents a quantitative immunoassay for prostate-specific antigen (PSA) using a surface-enhanced Raman scattering (SERS) substrate comprised of polymer-silver nanocubes (Ag NCs) modified with 4-mercaptobenzoic acid (4MBA). We investigated the impact of thiol-containing internal standards (IS) on the quantitative accuracy of SERS, highlighting the superior stability of 4MBA compared to non-thiol molecules like crystal violet (CV). In contrast to CV, 4MBA exhibited significantly greater stability, with only a 9.3% decrease in characteristic peak intensity after prolonged storage. Furthermore, the quantitative detection of PSA was realized using an IS-mediated sandwich immunostructure. Particularly, the coefficient of determination (R 2 ) for the PSA standard calibration curve demonstrated remarkable enhancement from 0.987 to 0.994, highlighting the advantage of thiol-anchored IS correction strategy. Moreover, the limit of detection (LOD) for PSA was refined to 5.6 × 10 -10 mg/mL after a perfect linear fitting was facilitated using 4MBA-based IS correction, which was superior to standard enzyme-linked immunosorbent assay (ELISA) and comparable SERS techniques. In addition, the specificity of the assay was confirmed by the lack of signal for non-target antigens. These findings demonstrate that the strategic selection of robust IS molecules can significantly enhance the quantitative capabilities of SERS technology, paving the way for its application in clinical diagnostics.
研究领域:
医学诊断
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纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2025
·
IF 5.4
Yi-Long Ma,Zhi Li,Qian-Lan Wu,Jing Li,Xin Guo,Tian-Le Mao,Kiran Thakur,Zhao-Jun Wei
3,5-O-Dicaffeoylquinic acid (3,5-DCQA) from edible Gongju chrysanthemum has shown protective effects against alcoholic hepatocyte injury in our previous study. However, the specific effects and mechanisms of 3,5-DCQA in a mouse model of liver injury remain underexplored. This study aimed to examine the efficacy and primary mechanism of 3,5-DCQA from Gongju chrysanthemum in ameliorating acute alcoholic liver injury in mice. The results indicated that 3,5-DCQA effectively attenuated inflammation and apoptosis by regulating the content of key factors (e.g. MyD88, NF-κB) in the TLR-4/NF-κB pathway, contributing to its protective role against alcohol-induced liver injury. 3,5-DCQA also inhibited inflammation and apoptosis by recovering the key metabolites (e.g. phosphatidylserine) and related genes (e.g. Lpgat1) of glycerophospholipid metabolism. These results were further verified by transcriptomic and metabolomic analyses, showing that 3,5-DCQA exerts protective effects against alcohol-induced liver injury by modulating glycerophospholipid metabolism and suppressing oxidative stress, inflammation, and apoptosis through the TLR-4/NF-κB pathway. The current findings highlight the hepatoprotectivity of 3,5-DCQA and may facilitate the application of Gongju chrysanthemum in liver-protective functional foods.
研究领域:
肝脏病学
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分子生物学
·
药理学
·
营养科学
·
代谢组学
·
转录组学
·
天然产物化学
·
氧化应激
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiao Jiang,Yutao Jian,Yuan Zhang,Juan Zhong,Qiulan Li,Xiaodong Wang,Xiaoshi Jia,Xiangnan Wu,Ke Zhao,Yitong Yao
The successful filling of bone defects remains challenging due to the incongruity between bone graft materials and the dynamic process of bone healing. Developing multifunctional materials matching the dynamic process of bone healing offers a viable solution to the current dilemma. Lines of evidence have shown that engineering osteoimmunomodulatory biomaterials can modulate the function of immune cells and thus promote bone regeneration. Herein, we utilized silk fibroin (SF) and polyglycolic acid (PGA) to create a PGA-SF core–shell fibrous scaffold, incorporating interleukin-4 (IL-4) and tricalcium phosphate (TCP) as a codelivery system (PGA/TCP-SF/IL-4), aiming to achieve an initial rapid release of IL-4 and sustained release of TCP. The PGA/TCP-SF/IL-4 scaffold mimicked the native bone structure and showed superior tenacity in the wetting regime. In vitro studies demonstrated that the PGA/TCP-SF/IL-4 scaffold significantly reduced the inflammatory response by upregulating the M2 macrophages, created a favorable microenvironment for osteogenesis, and facilitated osteogenic differentiation and mineralization. Implantation of the PGA/TCP-SF/IL-4 scaffold into the rat skull defect model notably increased the formation of new bones. IL-4 and TCP acted synergistically in attenuating inflammation and enhancing osteogenic differentiation. Overall, this multifunctional scaffold comprehensively considers the various demands in the bone defect region, which might have a significant potential for application in bone reconstruction.
研究领域:
生物材料
·
组织工程
·
骨愈合
·
免疫学
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
Optical Materials Express
·
2021
·Vol. 11
·期 9
·3015-3022
·
IF 3.442
Xiangfa Kong,Chaohui Cheng,Qifan Wang,Mianwu Meng,Mianwu Meng,Chaoshu Chen,Xiujian Wang,Siyu Huang,Siyu Huang,Siyu Huang,Weixin Gan,Weixin Gan
A Au-Ga alloy layer is synthesized on a Au sheet substrate at low temperature and a new nanoporous Au material is then prepared based on Ga removal from the Au-Ga alloy by a electrochemical method. X-ray diffraction analysis confirms that the grains of the newly formed Au layer are refined and scanning electron microscopy suggests that the newly formed Au layer has a nanoporous structure with a pore size of∼16 nm. The cyclic voltammetry curve of the prepared nanoporous Au in a 1 M KCl solution presents a square structure of a supercapacitor with a specific capacitance of mF·cm-2, which is 86 times that of the smooth Au electrode. The 4-mercaptobenzoic acid (4-MBA) modified nanoporous Au material is used as a Raman substrate to detect Hg2+ with surface-enhanced Raman scattering. from 1.0×10−10 mol·L-1 to 1.0×10−5 mol·L-1), and the limit of detection is 1.0×10−10 mol·L-1.
研究领域:
材料科学
·
分析化学
·
纳米技术
✓ 已验证
DOI
高影响因子
Nano Research
·
2024
·
IF 9.5
Zhu Shengjie,Huang Danqing,Luan Qichen,Li Yang,Gan Jingjing,Zhao Yuanjin,Sun Lingyun
Macrophages play a pivotal role in systemic lupus erythematosus (SLE) therapy. Efforts have been made to develop multifunctional drug delivery systems capable of directing macrophage polarization. Here, we present a novel hyaluronic acid methacrylate (HAMA) hydrogel microparticle encapsulating multiple cytokines for SLE remission though enhancing macrophage functions. The HAMA microparticles loaded with monocyte chemotactic protein-1 (MCP-1) and interleukin-4 (IL-4) were fabricated by using a microfluidic technology. The released MCP-1 facilitates the aggregation of inflammatory macrophages, after which IL-4 induces the macrophage phenotype shift from inflammatory M1 to immune-protective M2, thus restoring immune balance. We have demonstrated in MRL/lpr mice that the hydrogel microparticles could improve their efficacy of intraperitoneal drug delivery, modulate immune function, and attenuate the disease symptoms. These results suggest that our proposed microparticles delivery platform has potential clinical value for treating autoimmune diseases.
研究领域:
药理学
·
免疫学
·
生物医学工程
·
药物递送系统
·
自身免疫疾病
✓ 已验证
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Chaofan Zheng,Huaili Zheng,Yongjun Sun,Bincheng Xu,Yili Wang,Xinyu Zheng,Yongjuan Wang
Poly(4-vinyl pyridine) decorated magnetic chitosan biopolymer (VMCP), as an absorbent and reductant, was prepared and used to remove hexavalent chromium (Cr(VI)) from aqueous solution. Compared with undecorated magnetic biopolymer, VMCP exhibited significantly improved removal performance under identical experimental conditions. The kinetics, isotherms, and thermodynamics of Cr(VI) adsorption onto VMCP were investigated. Results demonstrated that the maximum monolayer adsorption capacity of VMCP was 344.83 mg/g, which was considerably higher than most reported adsorbents. The mechanism for Cr(VI) removal was explored based on XPS and FTIR analyses. The main mechanisms were concluded to be Cr(VI) adsorption onto the positively charged VMCP surface and the reduction of Cr(VI) to Cr(III), followed by coordination between Cr(III) and N atoms. The easy regeneration, satisfactory reusability, and remarkable performance in column tests revealed the high potential of VMCP in treating Cr(VI)-contaminated water.
研究领域:
环境科学
·
材料科学
·
化学工程
·
水处理
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-11
·
IF 5.833
He Yanlei,Li Ning,Liu Xiangwei,Chen Wei,Zhu Xixi,Liu Qingyun
Urchin-like peroxidase mimics 5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin–functionalized CuCo 2 O 4 nanospheres (Por-CuCo 2 O 4 ) has been fabricated as an excellent visual biosensor. X-ray diffractometry (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) have been employed to characterize the composition, morphologies, and elemental analysis of the as-synthesized Por-CuCo 2 O 4 . The catalytic activity of Por-CuCo 2 O 4 was evaluated by the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) with the aid of H 2 O 2 , which exhibited a visual blue change with an absorption maximum at 652 nm for only 10 s. The peroxidase-like behaviors of Por-CuCo 2 O 4 conformed to the Michaelis-Menten equation. Electrochemistry, radical scavenger, and fluorescence probe experiments verified that electron transfer, •O 2 − radicals, and holes (h + ) are the important factors during the catalytic oxidation of TMB. Based on the inhibition of dopamine (DA) on TMB oxidation, the Por-CuCo 2 O 4 -based colorimetric biosensor has been successfully constructed for sensitive determination of DA witha detection limit (LOD) of 0.94 μΜ. In addition, colorimetry was validated to detect DA in serum samples with high sensitivity and good selectivity. Graphical abstract
5,10,15,20-tetrakis (4-carboxyl phenyl) porphyrin–functionalized urchin-like CuCo 2 O 4 (Por-CuCo 2 O 4 ) with excellent peroxidase activity, ascribed to the synergistic effect between •O 2 − radicals and holes (h + ). A fast colorimetric sensor on the basis of Por-CuCo 2 O 4 has been constructed to quantitatively determine dopamine concentration in human serums.
研究领域:
纳米技术
·
生物传感器
·
分析化学
·
材料科学
·
生物化学
✓ 已验证
DOI
高影响因子
INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Jin Yang,Jianhao Zha,Qinan Mao,Heyi Yang,Lang Pei,Jiasong Zhong
Indoor plant cultivation has been receiving increased attention due to concerns about sustainable agricultural production. Phosphor-converted light-emitting diodes (pc-LEDs) offer substantial promise as lighting solutions for regulating plant growth. However, the quest for high-performance phosphors with precise responsiveness to far-red phytochromes poses a challenge. Our study proposes a chemical substitution strategy to develop near-infrared (NIR) phosphors, YAl3–xGax(BO3)4:Cr3+. Benefiting from Ga3+ replacement of Al3+, a red shift in the NIR emission of YAl3–xGax(BO3)4:Cr3+ is realized to attain a good alignment with far-red phytochrome absorption centering at 730 nm. The optimized YAl2.5Ga0.5(BO3)4:Cr3+ exhibits a favorable quantum yield of 71.8% and robust thermal stability of 101.0%@423 K, owing to local structural distortion and thermal repopulation caused by Ga3+ incorporation. The fabricated pc-LED combined with the phosphor yields an NIR output power of 40.9 mW and a photoelectric conversion efficiency of 15.4% at 100 mA. Plant growth experiments demonstrate that under NIR illumination from the devices, the average lengths of pea roots and stems gain 127 and 225% promotion, respectively, further highlighting the potential of YAl2.5Ga0.5(BO3)4:Cr3+ phosphors for applications in plant cultivation.
研究领域:
材料科学
·
光电子学
·
园艺照明
·
固态照明
·
光生物学
✓ 已验证
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Shiyang Zhou,Gangliang Huang
In this study, a series of novel 7 H -benzo [ c ] [1,3] dioxolo [4, 5- f ] chromen-7-one derivatives were obtained by structural modification of the lead compounds with Fissitungfine B. A total 15 compounds were designed, synthesized and evaluated as inhibitors of tumor. These target compounds have the novel chemical structures that named three six-membered rings including one lactone six-membered ring. In vitro assay, the results showed that the target compounds have a broad spectrum and strong of anti-tumor activity. Such as the target compound 4n to MCF-7 was IC 50 = 0.35 ± 0.01 μM, to A-549 was IC 50 = 0.37 ± 0.01 μM, to Hela was IC 50 = 0.56 ± 0.02 μM, to MDC-803 was IC 50 = 0.53 ± 0.02 μM and COLO-205 was IC 50 = 0.50 ± 0.02 μM in vitro . At the same time, in vivo anti-tumor activity assay results showed that the target compounds had a good inhibitory effect on tumor growth. Among them, the target compound 4n had the best anti-tumor activity, it could inhibit tumor growth well at a low dose. The target compound 4n could be used as a candidate drug for further research and development, in order to be used as early as application in the clinical treatment of tumors.
研究领域:
药物化学
·
肿瘤学
·
有机合成
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2012
·
IF 4.531
Lin-feng Zhou,Xu-gan He,Jun-qin Qiao,Hong-zhen Lian,Xin Ge,Hong-yuan Chen
A simple and facile needle-adapter was designed for constructing manual on-line polymer monolith microextraction-high performance liquid chromatography (PMME-HPLC). A capillary poly(4-vinylpyridine- co -ethylene glycol dimethacrylate) [poly(VP- co -EGDMA)] monolith was prepared by in situ polymerization, using 4-vinylpyridine (VP) and ethylene glycol dimethacrylate (EGDMA) as functional monomer and cross-linker, respectively. The synthesized monolith was used as the extraction medium for concentrating four EPA priority pollutants, 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol, from water samples. The effect of the dosage of porogen polyethylene glycol 6000 (PEG) on back pressure and extraction performance of the capillary monolith was also investigated. Moreover, the influences of several parameters (such as extraction time, desorption time, content of MeOH in sample solution and sample pH) were examined to obtain the optimal PMME conditions. As a result, the established on-line PMME-HPLC protocol, with good extraction efficiency (80.6–91.7%), satisfactory recovery (94.7–106% and 76.8–86.3% for water and serum samples, respectively) and low detection limit (0.3–1.4 μg/L), exhibited potential applicability for the analysis of chlorophenols in environmental and biological samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）