Journal of Pharmaceutical Innovation
·
2026
·Vol. 21
·期 6
·605
·
IF 2.6
Feng Yuxuan,Zuo Guanglei,Gong Xingchu
To address the industrial pain points of low automation, excessive manual intervention and poor process controllability in the traditional batch concentration process for traditional Chinese medicine (TCM) extracts, an automated continuous concentration apparatus was developed and its performance was validated in design space in this study. Adopting a modular design, the apparatus integrates a solvent recovery module, a heating evaporation module, a concentrated liquid detection module, and a central control module, which enables continuous feeding and discharging under negative pressure as well as continuous solvent discharge. Using the water extract of Lonicerae japonica as the model drug, a definitive screening design (DSD) was used to investigate the effects of stirring speed, feeding pump rate, temperature, vacuum degree, and liquid level height on evaporation rate and concentration ratio. Critical process parameters (CPPs) were identified, quadratic polynomial mathematical models were established, and the process design space was calculated and validated. The results showed that feeding pump rate and temperature were the CPPs affecting concentration efficiency; the maximum evaporation rate and concentration ratio achieved in the experiments were 529 mL/h and 2.71, respectively. The coefficient of determination (R²) of the evaporation rate model was 0.84, and the R² of concentration ratio model was 0.9519, with the predicted values of the models being in good agreement with the experimental validation results. The apparatus has a material processing capacity of 0.06–3 kg/h and enables stable and controllable continuous operation. This apparatus provides a miniaturized and intelligent experimental platform for the continuous concentration of TCM extracts, and also offers hardware support for the development of continuous manufacturing technology for TCM.
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DOI
高影响因子
Advanced Materials Interfaces
·
2026
·
IF 4.5
Yan Xue,Kai Zheng,Yang Liao,Jing Cao,Xupeng Yuan,Fusong Yuan,Yuxin Leng
Cracks in the tooth enamel may lead to cracked tooth syndrome, posing a potential threat to overall health. Hydroxyapatite (HAP) is the principal component of tooth enamel. In this paper, the repair of initial crack on the surface of HAP are demonstrated using an ultraviolet picosecond laser. The influences of laser processing parameters on the widths of dendritic cracks after laser melting repair were investigated. Although the laser repair results in the formation of dendritic cracks, the width of dendritic crack is submicron-scale. The localized temperature rise of HAP during laser repair is 3.5 ± 0.2 °C, which could effectively avoid the dental pulp damage in the clinical application. Moreover, the average hardness of HAP specimens significantly increases after laser repair (from 188.3 ± 37.2 HV to 501.9 ± 86.1 HV). With an optimized set of laser parameters, the proposed method has potential for rapid repair of early cracked teeth.
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Laibo Song,Yunchao Hu,Hengjie Liu,Zhen Jin,Jinlong Chang,Wenlong Zhang,Xianglong Zhu,Lanqi Jia,Wu Ren,Yi Yu
An interface-driven antibacterial platform is built using spiky MnO 2 microparticles. • Topology-associated mechano-bactericidal effects are combined with nanozyme activity. • The platform inactivates bacteria, inhibits biofilm formation, and partially disrupts mature biofilms. Bacterial infections, particularly those associated with biofilms, remain difficult to treat because bacteria are protected by the extracellular polymeric matrix and the local microenvironment. In this work, we developed spiky manganese dioxide (SP-MnO 2 ) microparticles serving as an interface-driven mechanochemical antibacterial platform combining topology-associated interactions with nanozyme activity. The spiky morphology facilitated bacterial capture and promoted close contact between bacteria and material, providing a structural basis for mechano-bactericidal interactions. At the same time, MnO 2 consumed glutathione and promoted reactive oxygen species generation through its nanozyme activity, thereby weakening antioxidant buffering and inducing oxidative damage near bacterial surfaces. Through this combined mechanochemical action, SP-MnO 2 achieved >97% antibacterial efficiency against planktonic bacteria, inhibited biofilm formation, and partially disrupted mature biofilms in vitro . Mechanistic comparisons further showed that the spiky morphology made a substantial contribution to the antibacterial activity by promoting bacteria-material interactions and localized oxidative damage. SP-MnO 2 also exhibited acceptable short-term cytocompatibility and hemocompatibility. Overall, this work introduces a proof-of-concept antibacterial platform and provides a rational strategy for enhancing antibacterial activity through morphology-regulated bacteria-material interfaces. An interface-driven mechanochemical antibacterial platform based on spiky MnO 2 microparticles combines topology-associated mechano-bactericidal effects with MnO 2 nanozyme activity. Download: Download high-res image (190KB) Download: Download full-size image
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DOI
高影响因子
MATERIALS & DESIGN
·
2026
·
IF 8.2
Fengbo Yu,Ying Zhu,Xingjun Fan,Chonghuan Lin,Guifang Liu,Jingdan Cao,Qiang Wang
A pH-responsive core–shell nanogel gave fourfold enhanced skin retention of esculetin. • It matched steroid efficacy and restored barrier proteins, a dual-action therapy. • A safe, scalable platform for natural compounds as topical anti-inflammatory agents. To address the need for topical therapies with enhanced skin penetration and retention for infantile atopic dermatitis (AD), we screened esculetin (Esc), an active monomer from traditional Chinese medicine, in a cellular AD model. Esc demonstrated pronounced anti-inflammatory activity. Subsequently, a self-assembled nanogel delivery system, Esc@(HAP/PSI)-gel, was engineered using hydroxyapatite (HAP) and polysuccinimide (PSI) for sustained release. The optimized nanoparticles were spherical (Average diameter: 156 nm, zeta potential: −15.7 mV) with pH-responsive drug release. Compared with Esc-gel, Esc@(HAP/PSI)-gel significantly enhanced skin permeation and drug retention without inducing irritation. In AD mice, Esc@(HAP/PSI)-gel alleviated skin lesions, reduced ear swelling, lowered the spleen index, attenuated epidermal/dermal thickening, suppressed inflammatory cell infiltration, and modulated key cytokines (IL-6, TNF-α, IgE) more effectively than Esc-gel. Hence, the HAP/PSI-based nanogel delivery system enables the efficient and sustained delivery of Esc, enhances transdermal absorption, and demonstrates considerable therapeutic potential for AD. Download: Download high-res image (259KB) Download: Download full-size image
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产品关联
DOI
高影响因子
Biomolecules
·
2026
·Vol. 16
·期 4
·554
·
IF 5.6
Xinhang Duan,Leting Wang,Chuxuan Cheng,Yili Zhang,Bingyue Guo,Hantong Wang,Jinghui Shi,Wenliang Song
Ocular infections and inflammation represent a clear risk to eye health, but standard eye masks often lack the necessary therapeutic features. Moreover, most existing studies employ a blended electrospinning approach, which leads to an inhomogeneous spatial distribution of the therapeutic agents. However, using the coaxial technique can address these limitations. This study develops a coaxial electrospun nanofibrous eye mask with dual antibacterial and antioxidant functions, aiming to provide an innovative ocular treatment tool for eye care. Generally, a core-shell structured bilayer polycaprolactone-polylysine/polyvinyl alcohol-resveratrol (PCL-PLs/PVA-RSV) membrane is successfully prepared by coaxial electrospinning, where the core is resveratrol-loaded PVA and the shell is PLs-loaded PCL. Results show uniform fiber morphology, favorable hydrophilicity, and potential for sustained release due to core-shell design. The membrane significantly inhibits the growth ofStaphylococcus aureus(S. aureus) andEscherichia coli(E. coli); at the same time, it exhibits excellent free radical scavenging ability and good component biocompatibility, achieving slow release of the two drugs and long-term antioxidant effect. This multifunctional platform offers a synergistic approach to combating microbial infection and oxidative stress, showing great potential for eye care.
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DOI
PubMed
高影响因子
NATURE BIOTECHNOLOGY
·
2026
·
IF 44.5
Yao Lang,Wang Zixuan,Chen Jialu,Sun Wen,Wang Kefan,Xiao Yunqi,Zhang Hanhan,Li Wenzheng,Wang Yifan,Zhao Lulu,Dai Xinyi,Qian Lu,Zhang Panke,Huang Shuo
Simultaneous characterization of proteins, RNAs and glycans with a nanopore remains a substantial challenge. To advance toward this goal, we introduce a multifunctional adaptor, maleimido-C2-formylphenylboronic acid (maleimido-C2-FPBA), into the Mycobacterium smegmatis porin A (MspA) nanopore at the pore constriction site. Here we demonstrate that the engineered nanopore, MspA-FPBA, enables the simultaneous identification of a diverse set of analytes, including 21 proteinogenic amino acids, three post-translationally modified (PTM) amino acids, four canonical nucleoside monophosphates (NMPs), three epigenetically modified NMPs, four monosaccharides and five peptides. Combined with machine learning, this sensor achieves an overall accuracy of 98.7%. Furthermore, it generates predictable event signatures for specific analyte types, enabling the machine learning model to identify analytes without strictly relying on prearchived event features, as demonstrated by the nanopore analysis of yeast cell extract. Lastly, we apply MspA-FPBA for compositional analysis of G2 glycopeptides. Future integration of hydrolase with MspA-FPBA may further improve MspA-FPBA utility as a single system to identify diverse biomolecules.
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DOI
高影响因子
Cell Reports
·
2023
·Vol. 42
·期 12
·113480-113480
·
IF 8.8
Liang Y,Meng F,Zhao X,He X,Liu J
The endoplasmic reticulum (ER) is the largest intracellular endomembrane system; it shows dynamic changes upon environmental stress. To maintain ER morphology and homeostasis under stress, the excessive ER membrane and the associated unwanted proteins can be removed via ER-phagy. Although a few ER-phagy receptors have been reported in mammals and yeast, their functional counterparts in plants remain largely unexplored. Here, we report that the HVA22 family protein OsHLP1 is an uncharacterized ER-phagy receptor in rice (Oryza sativa L.). OsHLP1 interacts with OsATG8b and recruits ER subdomains and the cargo protein OsNTL6, a negative immune regulator, to autophagosomes upon infection with the fungus Magnaporthe oryzae, which substantially activates disease resistance in rice. AtHVA22J, an Arabidopsis thaliana OsHLP1 ortholog, induced similar ER-phagy in plants. Altogether, we unraveled a conservative protein family that may act as ER-phagy receptors in higher plants, and in particular, we highlighted their roles in rice immune responses.
研究领域:
植物生物学
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分子生物学
·
植物病理学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Li Sun,Buhong Gao,Weina Jiang,Li Xu,Wen Lu,Shilong Yang,Dong Jiang,Jichao Chen,Huayu Xue,Jiuzhou Shi
Isorhamnetin as a natural product has excellent biological activity, but there are few reports of it in electrochemical applications. In this paper, we developed a neoteric electrochemical sensor for sensitive detection of mercury ions (Hg 2+ ) for the first time using glassy carbon (GC) electrode which was modified with isorhamnetin (ISO) and graphene oxide (GO). The electrochemical behaviors of the fabricated GC electrode were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under optimal conditions, the linear range to detect Hg 2+ with DPV was from 1.0×10 -6 to 1.0×10 -4 M with limit detection of 2.3×10 -8 M (S/N=3). In addition, it also showed excellent selectivity of Hg 2+ with comparison to several inorganic salt ions and other metal ions. We also report the application for Hg 2+ detection in real water samples with a good recovery (92.1-104.7%). In conclusion, isorhamnetin can be applied in the electrochemical field successfully with extraordinary results.
研究领域:
分析化学
·
环境科学
·
生物医学工程
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Jia Liu,Run-Tian Ma,Yan-Ping Shi
With the increasing demand for lipase inhibitors and new drugs used in the clinical treatment of obesity, it is of great significance to screen lipase inhibitors from traditional Chinese medicines (TCMs) via capillary electrophoresis. In this work, Fe 3 O 4 @TiO 2 nanoparticles was fabricated by solvothermal method and employed as an improved magnetic support to immobilize lipase through electrostatic interaction. By the method of transmission electron microscopy, fourier transform infrared spectroscopy and X-ray diffraction, the magnetic nanoparticles were characterized. The immobilized enzyme possessed advantages of a wider range for pH and temperature endurance, better storage stability and reusability. The kinetics performances of the immobilized lipase were studied. When p-Nitrophenyl palmitate (pNPP) was used as enzyme substrate, the Michaelis-Menten constant was calculated to be 2.51 mM and its inhibition constant for Orlistat was ascertained to be 13.41 μM. Ultimately, the established method was applied to lipase inhibitors screening from 6 Tibetan medicines with lipase inhibitory activity and Oxytropis falcate Bunge was screened out for its supreme lipase inhibitory activity. 11 compounds in the Oxytropis falcate Bunge were further screened, five compounds exhibited similar inhibitory activity to Orlistat, and one compound (kaempferol) presented better inhibitory activity than Orlistat, which is the most commonly used drugs to treat obesity in clinic. This work not only developed a method for new anti-obesity drugs discovery, but also provided inspiration for exploring new medicinal value of the TCMs.
研究领域:
药学
·
生物技术
·
中药学
·
药物发现
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DOI
PubMed
高影响因子
Breast Cancer-Targets and Therapy
·
2023
·
IF 2.6
Mengqi Zhang,Shengxian Bao,Guanhua Qiu,Jingchen Liang,Qin Wang,Xiaoqi Zhu,Guchun Qin,Junjie Liu,Chang Zhao
Purpose In this experiment, we constructed a magnetic targeting nano-diagnosis and treatment platform of doxorubicin (DOX) combined with iron nanoparticles, and explored their application value and mechanism in the treatment of Triple Negative Breast Cancer (TNBC), as well as its new diagnosis and treatment mode in Magnetic Resonance Imaging (MRI).Patients and Methods Hollow mesoporous nanoparticles (HFON) were synthesized by solvothermal method, and loaded the drug DOX (DOX@HFON) to treat TNBC. The experiments in vivo and in vitro were carried out according to the characteristics of the materials. In vitro experiments, the killing effect of the drug on cells was verified by cell viability CCK8, ROS generation level, LPO evaluation and flow cytometry; the MRI effect and targeted anti-tumor therapy effect were studied by in vivo experiments; then the tumor tissue sections were detected by Ki-67, CD31, ROS, LPO and TUNEL immunofluorescence detection; H&E staining and blood biochemical tests were used to evaluate the biosafety of the materials.Results Through a series of characterization tests, it is confirmed that the nano-materials prepared in this experiment have positive drug loading properties. MDA-MB-231 cells had great phagocytic ability to DOX@HFON under Confocal Laser Scanning Microscope (CLSM). Experiments in vitro confirmed that DOX and Fe were released and concentrated in cells, and a large number of ROS production and induction of LPO were detected by DCFH-DA and C11-BODIPY probes in cells. Apoptosis experiments further confirmed that DOX@HFON induced apoptosis, autophagy and ferroptosis. In the vivo experiment, the anti-tumor therapy effect of MAGNET@DOX@HFON group was the most significant, and in MRI also proved that the drug had great tendency and imaging ability in tumor tissue.Conclusion The new magnetic targeting nano-diagnosis and treatment platform prepared in this experiment is expected to become a new treatment model for TNBC.
研究领域:
癌症研究
·
纳米技术
·
分子生物学
·
成像技术
·
药理学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 40
·5496-5504
·
IF 0
Hongshi Zhu,Qingrui Geng,Wenqin Chen,Yunqing Zhu,Jing Chen,Jianzhong Du
Presented in this paper is an “armed” high-genus block copolymer vesicle (g = 18) which has excellent blood compatibility and more internal barriers than simple polymer vesicles (g = 0) for controlled anti-cancer drug delivery. The high-genus vesicle also shows better antibacterial activity against both Gram-positive and Gram-negative bacteria without quaternary ammonium moieties or the loading of any external antibiotics compared to the non-self-assembled individual polymer chains, or a conventional simple vesicle. This high-genus polymer vesicle was prepared by the self-assembly of PMEO2MA20-b-PTA20 diblock copolymers in DMF–water, where PMEO2MA is thermo-responsive poly[2-(2-methoxyethoxy)ethyl methacrylate] and PTA is pH-responsive and antibacterial poly[2-(tert-butylaminoethyl) methacrylate]. Doxorubicin (DOX) loading/release experiments revealed a retarded release rate of DOX in high-genus block copolymer vesicles than conventional simple vesicles, which could be used as an efficient drug delivery carrier with more internal barriers for drug molecules than conventional simple vesicles. Moreover, this “armed” drug delivery vehicle makes antibacterial and anti-cancer therapeutic processes proceed spontaneously, representing a safer and more efficient drug delivery system in nanomedicine.
研究领域:
生物材料学
·
纳米医学
·
药剂学
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DOI
NANOTECHNOLOGY
·
2012
·Vol. 23
·期 50
·505705
·
IF 3.979
Tao Chen,JiaJun Fu
The hollow mesoporous silica nanoparticles (HMSNs), which have been used as the nanocontainers for the corrosion inhibitor, benzotriazole, were fabricated using the hard-template method. Alkaline-responsive HMSNs based on cucurbit[6]uril (CB[6])/bisammonium supramolecular complex and acid-responsive HMSNs based on α-cyclodextrin (α-CD)/aniline supramolecular complex, which operate in water, have been achieved and characterized by solid-state NMR, thermogravimetry analysis, scanning electron microscopy, transmission electron microscopy and N2 adsorption-desorption analysis. The two elaborately designed nanocontainers show the pH-controlled encapsulation/release behaviors for benzotriazole molecules. Equal amounts of the alkaline- and acid-responsive nanocontainers were uniformly distributed in the hybrid zirconia-silica sol–gel coating and thus formed the intelligent anticorrosion coating. The self-healing property of AA2024 alloy coated with the intelligent anticorrosion coating is evaluated by electrochemical impedance spectroscopy (EIS). The sol–gel coating doped with the pH-responsive nanocontainers clearly demonstrates long-term corrosion protection performances when compared to the undoped sol–gel coating, which is attributed to the release of corrosion inhibitor from the nanocontainers after feeling the changes of environmental pH values near the corroded areas.
研究领域:
材料科学
·
腐蚀与防护
·
纳米技术
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DOI
高影响因子
危险品化学品经营许可证（带存储）