ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Xiangyu Ma,Kun Qian,Onome Ejeromedoghene,Martha Kandawa-Schulz,Yihong Wang
MicroRNAs (miRNAs) are becoming biomarkers for the clinical diagnosis of many diseases. However, their intrinsic properties such as low enrichment and short sequence restrict their applications. Here, we designed an ultrasensitive dual signal amplification electrochemical biosensor based on sodium alginate-polypyrrole/Au nanoparticles (SA-PPy/AuNPs) nanocomposite for the detection of miRNAs. Firstly, a planar intercalated copper(II) complex was synthesized to be the signal molecule. Then, catalytic hairpin assembly (CHA) reaction and catalytic reaction between ferric ion and copper ion were combined as a dual signal amplification to improve the sensitivity of the biosensor. In the presence of target miRNAs, multiple double helix structure formed for the CHA reaction was triggered, which immobilized more copper(II) complex on the surface of the working electrode and a strong signal was obtained in the square wave voltammetry (SWV) test. Also, the signal can be re-amplified again in the presence of ferric ion in the electrolyte. Under the optimal conditions, based on these strategies, a sensitive electrochemical biosensor was constructed for the detection of miRNAs in the range of 1 fM to 1 nM and the limit of detection was as low as 0.34 fM. Furthermore, the developed biosensor with excellent selectivity and reproducibility tested on human serum samples shows broad application prospects in the early stage of tumor diagnosis.
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高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Xiang-Xiu Chen,Zheng-Zhong Lin,Cheng-Yi Hong,Qiu-Hong Yao,Zhi-Yong Huang
A dichromatic label-free aptasensor was described for sulfadimethoxine (SDM) detection. Compared with the binding of SDM-aptamer to SDM, the higher affinity of aptamer to cDNA may result in the hybridization of dsDNA. In the presence of SDM, the aptamer specifically binds to SDM, leading to a blue color of AuNPs in deposit and fluorescence at 530 nm in supernatant after adding cDNA and SGI. With no target of SDM, AuNPs protected with the aptamer re-disperse in PBS with a red color, and no fluorescence occurs in supernatant. Based on the principle, SDM can be quantitatively detected through both fluorescent emission and AuNPs color changes with recoveries ranging from 99.2% to 102.0% for fish and from 99.5% to 100.5% for water samples. An analytical linear range of 2–300 ng mL −1 was achieved with the detection limits of 3.41 ng mL −1 for water and 4.41 ng g −1 for fish samples (3 σ , n = 9).
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Yunuo Dai,Jie He,Yun Zhou,Yanyan Yu,Hui Hui,Lin Guo,Haitao Yin
Transformation of epithelial to mesenchymal (EMT) is an important event in the process of tumor initiation, invasion and metastasis. Circulating tumor cells (CTCs) are one kind of important markers in the field of liquid tumor biopsy, whose number and phenotype represent the occurrence and progression of tumors. Therefore, it is our interest to investigate the epithelial mesenchymal transition process occurring on the surface of CTCs. Herein in this work, two proteins of E-cadherin (E-cad) and N-cadherin (N-cad) were selected as representative proteins of EMT process. To achieve simultaneous analysis of E-cad and N-cad on the surface of rare CTCs, we designed a duplex and portable immunosensor using AuNPs and AgNPs as nanolabels to amplify the immunoreaction signals. The dual channel immunosensor not only exhibited good electrochemical responses for recombinant E-cad and N-cad as low as 0.1 ng/mL and 0.05 ng/mL, respectively, but also showed good linear correlations with different numbers of phenotypic CTCs (10–500 cells/10 μL). The above strategy was further employed to inspect the occurrence of EMT on CTCs surface, which displayed a high consistence with other molecular biological characterizations. Finally, this immunoassay was successfully applied to inspect the correlations of numbers, phenotype of CTCs, as well as E-cad and N-cad expressions on these CTCs in bloods of NSCLC patients with disease stage.
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PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 91
·74548-74556
·
IF 3.84
Jianfeng Fei,Wenchao Dou,Guangying Zhao
A rapid and highly sensitive sandwich electrochemical immunoassay method was constructed for Salmonella pullorum and Salmonella gallinarum (S. pullorum and S. gallinarum) determination based on immune magnetic beads (MBs) and an enzyme labeled antibody. An abundance of gold nanoparticles (AuNPs) were attached to SiO2 coated Fe3O4 nanoparticles (Fe3O4/SiO2) via covalent binding between the –SH groups of Fe3O4/SiO2 and the AuNPs. Antibodies against S. pullorum and S. gallinarum were immobilized on Fe3O4/SiO2/AuNPs nanocomposites (AuMNPs) by automatic adsorption between thiol and the AuNPs. S. pullorum and S. gallinarum in the sample were captured by the AuMNPs and separated from the samples by applying an external magnetic field. The AuMNPs–Salmonella complexes (Ag/Ab1/AuMNPs) were re-dispersed in a buffer solution then exposed to horseradish peroxidase labeled anti-S. pullorum and S. gallinarum (HRP-Ab2) solution, forming a sandwich-type immune complex (HRP-Ab2/Ag/Ab1/AuMNPs). A 4 channel screen printed carbon electrode (4-SPCE) was modified by gold nanoparticles (AuNPs) through the electrodeposition method to prepare AuNPs/4-SPCE. After magnetically separating the sandwich immune complexes from solution, HRP-Ab2/Ag/Ab1/AuMNPs was anchored on AuNPs/4-SPCE by a magnet. A linear response to S. pullorum and S. gallinarum was obtained in the concentration range from 102 to 106 CFU mL−1, with a limit of detection of 3.2 × 101 CFU mL−1 (at an SNR of 3). This nanoparticle-based immunoassay method offers sensitive, highly specific, and reproducible detection of S. pullorum and S. gallinarum. Given its low detection limit, it represents promising potential in the detection of other food-borne pathogens by exchanging the antibody.
研究领域:
生物传感器
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食品安全
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电分析化学
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纳米生物技术
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微生物检测
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiaoyu Yang,Shanshan Li,Yang Yang,Xurong Huang,Linhui Li,Wenjin Yang,Zhiqing Zhang,Guanghui Shen,Xiaoyan Hou,Qiang Cui,Anjun Chen
In this study, we developed two novel electrochemical sensors for the detection of hydroxy-α-sanshool (HαSS) and hydroxy-(α+β)-sanshool (H(α+β)SS), based on the molecularly imprinted AuNPs/Fe-MOF polymer composites films. The imprinted films were facilely constructed via in situ electrodeposition of gold nanoparticles (AuNPs) on the Fe-MOF coatings, followed by a simple electrochemical polymerization process. The AuNPs/Fe-MOF composites not only enhanced the electroactive surface areas but also improved the conductivity and the stability of the polymerized films. Under optimal conditions, the HαSS sensor demonstrated excellent performance, with a detection range of 1–120 μM and a detection limit of 0.206 μM. The sensor for H(α+β)SS, reported for the first time, exhibited a broad linear range of 0.11–132 μM and accurately detected total HαSS and HβSS, achieving a detection limit of 0.072 μM. Both sensors performed effectively in the detection of HαSS or H(α+β)SS in Zanthoxylum bungeanum Maxim. and its products. The two developed sensors significantly outperform other previously reported sanshool sensors and show previously potential for applications in the Zanthoxylum industry.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Tai-Cheng Lu,Yi-Ting Lin,Wen-Biao Xiao,Qing-Zhen Qiu,Hui-Yun Tian,Yun Lei,Ai-Lin Liu
Developing the portable CRP detection technologies that are suitable for point-of-care (POC) and primary care management is of utmost importance, and advancing the electrochemical immunosensors hold promise for POC implementation. Nevertheless, non-specific adsorption of numerous interfering proteins in complex biological media contaminates immunosensors, thereby restricting the reliability in detection efficacy. In this study, a three-dimensional flower-leaf shape amyloid bovine serum albumin/gold nanoparticles/polyaniline (AL-BSA/AuNPs/PANI) coating on the surface of the electrode was developed, which demonstrated strong anti-adsorption properties against bovine serum albumin, plasma, and cells. The immunosensor exhibited a good linear relationship to CRP response, featuring a detection limit of 0.09 μg/mL, consistent with clinical reference range. In addition, the CRP immunosensor demonstrated excellent specificity in other inflammation-related proteins and commendable anti-interference performance for CRP detection in plasma and whole blood tests. Importantly, by combining the development of a USB flash disk-type portable electrochemical workstation with a reagent-free mode, the developed CRP electrochemical immunosensor delivered ideal results in clinical samples. The anti-fouling performance, sensitivity and specificity of the immunosensor, as well as its flexible test modes in clinical samples, provide important scientific basis for developing POC detection technologies of vital biomarkers in complex biological media.
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Zhiren Zhou,Lina Zou,Ping Zhang,Chunmei Li,Ye Zhu,Huanyu Ren,Zheng Li,Huiru Niu,Hao Liao,Xiaojing Zhang,Shanshan An,Fei Ren,Xiuhong Ge,Lang Cheng,Feiyan Yang,Hongzhi Pan,Shengzhong Rong,Dong Chang,Hongkun Ma
Survivin serves as a crucial biomarker for bladder cancer, so exploring the detection method is essential. Therefore, a sensitive ratiometric electrochemical immunosensor was developed. In brief, a double-layered amino graphene oxide (NH 2 -GO) sandwich enhanced by Fe-Ni@Ti 3 C 2 T x (NH 2 -GO/Fe-Ni@Ti 3 C 2 T x /NH 2 -GO) significantly amplified the detection signal. ferrocene@Au nanoparticles (Fc@AuNPs) provided a reference signal, and Ab 1 was anchored via an Au-N bond. Moreover, AuPt-methylene blue (AuPt-MB) served not only as a secondary signal but also anchored Ab 2 through Au-NH 2 and Pt-NH 2 bonds. Black phosphorus nanoplates (BPNPs) improved conductivity and provided additional binding sites for AuPt-MB. The immunosensor exhibited a broad detection range (0.001~1000 ng/mL) and a low detection limit (0.00065 ng/mL), facilitating successful survivin detection in human urine samples with promising clinical analysis applications.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 57
·期 8
·5376-5387
·
IF 3.612
Hai Chi,Yujie Li,Guoqin Liu
Summary In this study, a new electrochemical strategy based on the fabrication of a molecularly imprinted sensor onto a MoS 2 -loaded peanut shell carbon complex with gold nanoparticles (AuNPs) and nitrogen-doped carbon dots (N-CDs) was proposed for the detection of benzo(a)pyrene (BaP). Molecularly imprinted polymer (MIP) films were prepared by cyclic voltammetry (CV) using 2-mercaptobenzimidazole (2-MBI) as a functional monomer in the presence of BaP. The surface morphologies, structural characteristics and electrochemical properties of the obtained MIP/AuNPs/N-CDs/PSBC/MoS 2 /GCE were investigated via scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectrometry (EDS), Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD), UV–Vis spectrometry, fluorescence spectrometry, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimised conditions, the detection range of the electrode towards BaP varied from 5 nM to 20 μM with a detection limit of 1.5 nM. The prepared electrochemical sensor also exhibited good stability, relevant reproducibility and high selectivity. The application of the sensor in the actual analysis of edible oil samples showed promising results, thereby being relevant as a biomimetic sensing platform for the detection of chemical hazards in food and environment.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Mingyue Wang,Hao Guo,Ning Wu,Junye Zhang,Tinging Zhang,Bingqing Liu,Zhilan Pan,Liping Peng,Wu Yang
In this work, a novel triazine-based covalent organic framework (TS-COF) was constructed by the condensation of 1, 3, 5-tris-(4-aminophenyl)triazine (TAPT) and squaric acid (SA) under solvothermal condition. By in situ growth of Au nanoparticles (AuNPs) on the surface of TS-COF, the AuNPs@TS-COF composite with enhanced electroactivity was prepared successfully. After introduction of reduced graphene oxide (RGO), the constructed AuNPs@TS-COF/RGO nanocomposite modified electrode exhibited excellent conductivity and outstanding catalytic activity, and could be employed to determine uric acid (UA), dopamine (DA) and ascorbic acid (AA) in urine sample simultaneously, accurately and rapidly with high sensitivity, good selectivity and high stability. The detection limits of UA, DA and AA were 0.07 μM, 0.03 μM and 4.30 μM (S/N = 3), respectively.
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高影响因子
ANALYTICAL LETTERS
·
2019
·
IF 1.248
Chunyan Wang,Hui Zhang,Xiaoqing Jiang,Bo Zhou
A sensitive electrochemical biosensor was designed for determination of aflatoxin B1 (AFB1) using a copper-based metal-organic framework (Cu-MOF), which has strong electrochemical activity and exonuclease III (Exo III)-assisted recycling for dual signal amplification. Hairpin DNA (S1) was immobilized on the electrode. The AFB1 was recognized by aptamer DNA (S2) and complementary DNA (S3) was released. The S3 hybridized with the hairpin S1 to form the Exo III hydrolyzed double-stranded DNA, leaving a partial sequence of hairpin DNA (S1′) on the electrode and releasing S3 for the next cycle of the opening and digestion of hairpin S1. The amplified S1′ then was able to combine with more signal probes. Cu-MOF bond gold nanoparticles (AuNPs) by -NH2 were immobilized to capture DNA (S4) to obtain Cu-MOF/AuNPs/S4. This signal probe Cu-MOF/AuNPs/S4 was able to hybridize with the electrode and generate an amplified electrochemical signal. Under the optimized conditions, this electrochemical biosensor for AFB1 exhibited a low detection limit of 6.7 × 10−7 ng/mL at a signal-to-noise equal to 3 and a wide linear range from 10−6 to 1 ng/mL. The biosensor was also used to analyze AFB1-spiked beer sample with recovery values between 96% and 103%. This method has the potential to become a valuable technology for detecting various toxins by the selection of the appropriate aptamer DNA.
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