MEDICAL SCIENCE MONITOR
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2020
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IF 1.918
Xiaolin Pu,Hua Jiang,Wei Li,Lin Xu,Lin Wang,Yongqian Shu
Background The coatomer protein complex subunit beta 2 (COPB2) gene is upregulated and promotes cell proliferation in some cancer cells. This study aimed to investigate the role of microRNA (miRNA) targeting by COPB2 gene expression in human lung adenocarcinoma cell lines, including NCI-H1975 cells. Material/Methods COPB2 expression in normal human bronchial epithelial cells and lung adenocarcinoma cells was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot. NCI-H1975 human lung adenocarcinoma cells were transfected with short-interfering COPB2 (siCOPB2). Cell apoptosis and cell proliferation were evaluated by flow cytometry and Cell Counting Kit-8 (CCK-8) assays, respectively. The transwell assay evaluated cell migration. Targeting of miR-335-3p by COPB2 was predicted using TargetScan 7.2 and verified using a dual-luciferase reporter assay in NCI-H1975 cells. MiR-335-3p mimics were transfected into NCI-H1975 cells. The further functional analysis included detection of protein expression for cyclin D1, tissue inhibitor matrix metalloproteinase-1 (TIMP-1), matrix metallopeptidase 9 (MMP9), Bcl-2, and Bax, to verify the role of miR-335-3p targeting by COPB2 in lung adenocarcinoma cells. Results COPB2 was upregulated in lung adenocarcinoma cells and was a direct target of miR-335-3p mimics. COPB2 knockdown promoted cell apoptosis, inhibited cell migration and proliferation in NCI-H1975 cells. The effects of COPB2 knockdown on NCI-H1975 cells were increased by miR-335-3p mimics, which also further reduced the expression levels of cyclin D1, MMP9, and Bcl-2 and further increased TIMP-1 and Bax by siCOPB2. Conclusions This study showed that COPB2 was the functional target of miR-335-3p in NCI-H1975 human adenocarcinoma cells.
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DOI
PubMed
高影响因子
MOLECULES
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2018
·Vol. 23
·期 10
·2663
·
IF 3.098
Tian Chai,Xiao-Bo Zhao,Wei-Feng Wang,Yin Qiang,Xiao-Yun Zhang,Jun-Li Yang
Gx-50 is a bioactive compound for the treatment of Alzheimer’s disease (AD) found in Sichuan pepper (Zanthoxylum bungeanum). In order to find a stronger anti-AD lead compound, 20 gx-50 (1–20) analogs have been designed and synthesized, and their molecular structures were determined based on nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis, as well as comparison with literature data. Compounds1–20were evaluated for their anti-AD potential by using DPPH radical scavenging assay for considering their anti-oxidant activity, thioflavin T (ThT) fluorescence assay for considering the inhibitory or disaggregate potency of Aβ, and transgenicDrosophilamodel assay for evaluating their rescue effect on memory loss. Finally, compound13was determined as a promising anti-AD candidate.Keywords:Alzheimer’s disease;gx-50;DPPH assay;ThT assay;transgenicDrosophilamodel assay
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DOI
PubMed
高影响因子
Molecular Catalysis
·
2019
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IF 2.938
Qiaoyun Liu,Fei Liu,Yaru Zhang,Yang Su,Aiqin Wang,Tao Zhang
A highly active and robust catalyst composed of Ru nanoparticles highly dispersed on zeolite H-beta was developed for transforming furfural into 3-acetyl-1-propanol (3-AP) in good yield (81%)under mild conditions (80 °C, 1 MPa H 2 ). Both the support nature and the preparation methods had significant impacts on the dispersion of Ru, eventually on the yield of 3-AP. Ion-exchange method proved best for obtaining the highly active and selective catalyst for this reaction. Moreover, the catalyst can be regenerated easily by calcination and reduction. This catalyst was further explored for the one-pot conversion of xylose and xylan to 3-AP with a yield of 46% and 31%, respectively, thus opening a new feasible pathway from biomass to valuable fine chemicals.
研究领域:
催化化学
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生物质转化
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绿色化学
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高影响因子
Foods
·
2021
·Vol. 10
·期 12
·2951
·
IF 4.35
Qian Li,Ying Zhao,Yanli Xie
Paeonol can effectively inhibitAspergillus flavus(A. flavus) via damaging cell walls. In this work, paeonol treatment remarkably destroyed both the outer amorphous layer and the inner fibrous layer of cell walls. Furthermore, FT-IR and XPS characterization showed that OH functional groups were altered and proteins in the outer layer were released. According to proteomic analysis, 605 proteins have been identified and annotated. The activities of β-1,3-glucan synthase and chitinase were prohibited and promoted, respectively, by paeonol treatment, however, the activities of β-1,3-glucanase and chitin synthase were not influenced. QRT-PCR results suggested thatFKSP,CHIIII, andCHIVgenes might be the antifungal targets of paeonol. In addition, paeonol can effectively restrain the pathogenicity ofA. flavuson peanut butter. This study provided a new elucidation on the mode of action of paeonol against cell walls ofA. flavus, facilitating the application of paeonol in the preservation of agricultural products.Keywords:paeonol;Aspergillus flavus;cell walls
研究领域:
微生物学
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真菌学
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食品科学
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药理学
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PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Yi Shi,Jingyi Zhang,Jia He,Donghao Liu,Xiaoyan Meng,Tao Huang,Hua He
Analyzing of tumor markers has become an important means for cancer diagnosis and prevention. In this study, a novel solid phase extraction based on porous magnetic cyclodextrin polymer (MA-CD) was developed and used for detection of trace small molecule gastric tumor markers in urine samples. The adsorption properties of the magnetic cyclodextrin polymer were tested. Through experiments of the solid phase extraction (SPE) at the different condition, the optimal condition was selected to test the two tumor markers by High-performance-liquid chromatography -Diode array detector (HPLC-DAD). The analytical performance of the method showed good accuracy (88.82%–104.34%) and precision (< 3.55%), appropriated detection limits (1.016 and 5.714 μg L −1 ) and linear ranges (0.6–24.0 μg L −1 ) with convenient determination coefficients (> 0.9994). The results demonstrated that the developed approach is efficient, low-cost for gastric tumor markers detection.
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高影响因子
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