JOURNAL OF APPLIED POLYMER SCIENCE
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2025
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IF 2.8
Ying Lei,Jianyang Zhang,Haoquan Zhuang,Tao Yin,Pengcheng Lin
High-power consumption causes excessive heat of 5G devices, compromising performance and lifespan. Phase change materials (PCMs) enable passive, constant-temperature thermal management but suffer from leakage and low thermal conductivity, limiting their application. In this work, a strategy of heat transfer enhancement in thermal energy storage gels by size engineering of boron nitride (BN) towards 5G thermal management is proposed. Specifically, tetradecanol (TD), whose thermal storage temperature is close to the thermal management temperature of electronic devices, is selected as the working medium. Based on the van der Waals force and hydrogen bond between TD and styrene-ethylene-butylene-styrene (SEBS), TD@SEBS@BN gels (TSBGs) with suppressed leakage are formed. The enthalpy of the TSBG is as high as 128.12 J g −1 , stably storing heat at around 35.84°C. The synergistic enhancement of heat transfer of TSBGs by large/small-sized BN is studied. An efficient heat transfer network has been established by coupling large-sized BN (average 30 μm) and small-sized BN (average 1 μm). The thermal conductivity of TSBG is increased from 0.304 W m −1 K −1 to a high value of 0.966 W m −1 K −1 (an increase of 317.76%). The efficient thermal management of 5G electronic components is finally realized using TSBGs.
研究领域:
热管理
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材料科学
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电子器件
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纳米技术
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传热
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MEASUREMENT
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2025
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IF 5.6
Xu-guang Hu,Zhan-kun Li,Ju-wei Zhang,Xi-xin Wang,Xiao-ming Chen,Yong Zhao
A label-free fiber-optic optofluidic biosensor integrated into microfluidic chip based on vernier effect is proposed for the specific and high-sensitivity detection of DNA molecules, which is made up of a Michelson interferometer (MI) and a Fabry Perot interferometer (FPI) in parallel. The MI is prepared by a single-hole dual-core micro-structured fiber (MOF) with a suspended core attached to the hole serving as the sensing arm, and used as the sensing element. A micropore is etched directly above the microchannel of MI as the fluid inlet by femtosecond laser processing, and integrated into the microfluidic chip for sample replacement. The detection volume is only nanoliters and the sample can be quickly replaced within seconds. The experimental findings indicate that the proposed optofluidic sensor shows a high refractive index (RI) sensitivity of −8791 nm/RIU and has an ultra-low detection limit of 7.45 × 10 −6 RIU within the range of 1.3328–1.3370. By immobilizing probe DNA (pDNA) molecules on the suspended core through surface functionalization, the optofluidic sensor enables label-free detection of 1 μM complementary DNA (cDNA) solution and shows excellent specificity. The proposed fiber optofluidic biosensor has great development prospects in ultra-low volume and high-sensitivity detection of biomolecules.
研究领域:
生物医学工程
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光学工程
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分子生物学
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Biosensors-Basel
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2021
·Vol. 11
·期 5
·162
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IF 5.519
Mathias Busek,Steffen Nøvik,Aleksandra Aizenshtadt,Mikel Amirola-Martinez,Thomas Combriat,Stefan Grünzner,Stefan Krauss
Polydimethylsiloxane (PDMS) has been used in microfluidic systems for years, as it can be easily structured and its flexibility makes it easy to integrate actuators including pneumatic pumps. In addition, the good optical properties of the material are well suited for analytical systems. In addition to its positive aspects, PDMS is well known to adsorb small molecules, which limits its usability when it comes to drug testing, e.g., in organ-on-a-chip (OoC) systems. Therefore, alternatives to PDMS are in high demand. In this study, we use thermoplastic elastomer (TPE) films thermally bonded to laser-cut poly(methyl methacrylate) (PMMA) sheets to build up multilayered microfluidic devices with integrated pneumatic micro-pumps. We present a low-cost manufacturing technology based on a conventional CO2laser cutter for structuring, a spin-coating process for TPE film fabrication, and a thermal bonding process using a pneumatic hot-press. UV treatment with an Excimer lamp prior to bonding drastically improves the bonding process. Optimized bonding parameters were characterized by measuring the burst load upon applying pressure and via profilometer-based measurement of channel deformation. Next, flow and long-term stability of the chip layout were measured using microparticle Image Velocimetry (uPIV). Finally, human endothelial cells were seeded in the microchannels to check biocompatibility and flow-directed cell alignment. The presented device is compatible with a real-time live-cell analysis system.Keywords:organ-on-a-chip;micro-pneumatics;PDMS-free;thermoplastic elastomers;layer-by-layer manufacturing
研究领域:
生物医学工程
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细胞生物学
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高影响因子
STAR Protocols
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2023
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IF 0
Zitong Yu,Rui Hao,Xi Chen,Lu Ma,Yi Zhang,Hui Yang
Summary Organs-on-chips are microfluidic devices for cell culturing to simulate tissue- or organ-level physiology, providing new solutions other than traditional animal tests. Here, we describe a microfluidic platform consisting of human corneal cells and compartmentalizing channels to achieve fully integrated human cornea’s barrier effects on the chip. We detail steps to verify the barrier effects and physiological phenotypes of microengineered human cornea. Then, we use the platform to evaluate the corneal epithelial wound repair process. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022). 1
研究领域:
生物医学工程
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组织工程
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细胞生物学
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PubMed
TALANTA
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2022
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IF 6.556
Huijun Lu,Jinhui Zhu,Tong Zhang,Xinlian Zhang,Xi Chen,Wei Zhao,YuHan Yao,Wang Zhao,Guodong Sui
Occupational health problems, such as asthma, in specific work environments arise from the presence of airborne fungi. Rapid detection of pathogenic airborne fungi is therefore important to reduce or avoid any adverse effects on staff health. Herein, we established a new integrated rapid L yticase- M otor- C hemical reagent nucleic acid releasing (LMC) method for the release of fungal DNA . Aspergillus fumigatus , Aspergillus flavus , and Cryptococcus neoformans were chosen to evaluate the LMC method. The results of Loop-Mediated Isothermal Amplification (LAMP) analyses showed that this method could release the nucleic acid of 4 × 10 4 fungal spores, equaling to 400 copies per microliter. This rapid multiplex nucleic acid detection system of airborne fungi included an integrated DNA release device and a portable microfluidic chip. The integrated DNA release device combined mechanical lysing and biochemical reagent treatment to automate DNA release. The microfluidic chip was capable of multiplex nucleic acid detection. The detection limit of this system was 4 × 10 4 spores per test, meeting the requirement of early warnings. The whole analysis from the sample input to readout could be completed within 90 min, including 30 min for fungal DNA release and 45 min for LAMP analysis. The integrated DNA release device and microfluidic chip were portable, showing tremendous potential in point-of-care tests of airborne fungi.
研究领域:
职业健康
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分子生物学
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微流控技术
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生物技术
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高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 4
·223
·
IF 5.4
Wang Tingyu,Xu Zefan,Wu Lei,Zhang Yu,Liu Hang,Wang Zuyao,Zong Shenfei,Yang Kuo,Wang Hong,Wang Zhuyuan
Exosomes hold considerable promise for early cancer diagnosis. However, their low abundance during early tumor progression and inefficient receptor binding remain obstacles to their broad application. Here, we present the surface acoustic wave (SAW)-SERS Exosome Capture Analysis Chip (SEC-chip), which enriches exosomes within 5 min and quantifies HER2 using only 5 µL of serum. The device features a reusable acoustic transducer coupled with a disposable, CD63 aptamer functionalized capture base. Acoustic microstreaming concentrates vesicles into the detection zone, enabling a detection limit of 5 particles/5 µL, representing a 100-fold improvement over static assays. This hybrid design eliminates cross-contamination and reduces costs. Validated on 12 clinical samples, the SEC-chip accurately stratified HER2-positive breast cancer patients. Integrating rapid processing with ultrasensitivity, the platform offers a robust liquid biopsy tool for tumor diagnosis and future point-of-care screening.
Section
Graphical
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Jove-Journal of Visualized Experiments
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2023
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IF 1.2
Wenwen Dong,Chunxian Tao,Bo Yang,Erika Miyake,Zhenqing Li,Dawei Zhang,Yoshinori Yamaguchi
Polymerase chain reaction (PCR) is a traditional method employed for the amplification of a target gene that has played an important role in biomolecular diagnostics. However, traditional PCR is very time-consuming because of the low-temperature variation efficiency. This work proposes a continuous-flow-PCR (CF-PCR) system based on a microfluidic chip. The amplification time can be greatly reduced by running the PCR solution into a microchannel placed on heaters set at different temperatures. Moreover, as capillary electrophoresis (CE) is an ideal way to differentiate positive and false-positive PCR products, a CE system was built to achieve efficient separation of the DNA fragments. This paper describes the process of amplification of Escherichia coli (E. coli) by the CF-PCR system built in-house and the detection of the PCR products by CE. The results demonstrate that the target gene of E. coli was successfully amplified within 10 min, indicating that these two systems can be used for the rapid amplification and detection of nucleic acids.
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SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Xueliang Lin,Youliang Weng,Yi Liu,Duo Lin,Huishan Yang,Zhiwei Chen,Shangyuan Feng
The rapid and high precision analysis of SARS-CoV-2 RNA in saliva is crucial for the efficient prevention and control of COVID-19. Herein, we developed a novel ratiometric surface-enhanced Raman spectroscopy (SERS) sensing chip based on a two-dimensional core-molecule-shell-molecule nanostructure for quantitative detection of SARS-CoV-2 RNA. Owing to the use of base-complementary pairing mechanism, this SERS sensing chip has better selectivity in discriminating SARS-CoV-2 RNA against other RNA. Besides, an internal standard molecule (4MBN) in the SERS sensing chip can used to calibrate the real-time changing Raman signals of the probe molecule during quantitative assay. Using this cleverly designed SERS sensing chip, the dynamic ranges from 10 –6 M to 10 –12 M with a low of detection limit (LOD = 7.61 ×10 –14 M) can be acquired for detection of SARS-CoV-2 RNA. Meanwhile, this SERS sensing chip was well adapted for point-of-care testing (POCT) in combination with portable Raman spectrometer, which is expected to achieve effective detection of early SARS-CoV-2 infection.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Ge Gao,Ji Qi,Longwen Fu,Rongfang Zhao,Zhiyang Zhang,Lingxin Chen
To ensure real-time validity of the detection of unstable toxic environmental pollutants, such as dissolved sulfides, we developed a portable on-site rapid analysis instrument. Through novel design of the color sensor chip-based core sensing components and the conversion between color signal and absorbance by Lambert's law , the instrument showed great performance for rapid (within 3 min) and sensitive on-site detection of sulfides in the environment. It is easy to achieve user-friendly, sample in-answer out, one-stop operation due to the touch-screen-integrated user interface of the instrument's data terminal. The detection limit of this method is 2.24 μg/L, the linear operation range is 0–1000 μg/L, and the coefficient of determination is 0.999. This instrument has been successfully applied to the on-site determination of sulfides in the Yellow River Delta and the Yantai Guangdang River in China. The portable instrument showed excellent anti-interference, good stability, and simple operation, which showed great prospects for the on-site rapid analysis of unstable targets in the environment.
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BIOSENSORS & BIOELECTRONICS
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2023
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IF 12.6
Wei Nie,Ruoxian Zhang,Chao Hu,Tengchuan Jin,Xi Wei,Hua Cui
SARS-CoV-2 antigen detection plays a key role in the rapid diagnosis of COVID-19. However, current clinically used immunoassays are often limited by assay throughput, sensitivity, accuracy, and field operating conditions. To address these challenges, we constructed a self-enhanced electrochemiluminescence (ECL) array chip (SE 2 AC) for highly sensitive and label-free detection of SARS-CoV-2 nucleocapsid protein (N protein) with a facile and portable assay setup. Firstly, the self-enhanced ECL nanomaterials with inherent film-forming properties were synthesized by co-doping Ru(bpy) 3 2+ and polyethyleneimine (PEI) in silica nanoparticles (Ru/PEI@SiO 2 ). Secondly, a resistance-induced potential difference-based single-electrode electrochemical system (SEES) was adapted to serve as the electrode array to facilitate one-step assembly without the need for chip alignment. Thirdly, the chip electrode array was functionalized with the synthesized self-enhanced ECL emitters and captured antibodies. In addition, a portable detection box equipped with a smartphone was 3D-printed to serve as the chip holder and “dark room” for imaging acquisition. The SE 2 AC performance was validated with N protein with a limit of detection (LOD) of 0.47 pg/mL in the range of 1–10,000 pg/mL. Furthermore, the chip successfully detected the viral antigen residue as low as 1.92 pg/mL from diluted rehabilitation patients’ serum samples. This is the first study reporting label-free detection of SARS-Cov-2 N protein based on a self-enhanced ECL immunosensor, which provides a novel facile method for highly sensitive diagnosis of COVID-19 with high throughput, portability, and low cost.
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Advanced Healthcare Materials
·
2023
·Vol. 12
·期 17
·2202904
·
IF 10
Yizuo Song,Xin Shou,Bo Sheng,Jie Mei,Keqing Shi,Luoran Shang,Xueqiong Zhu
Nanoparticles (NPs)-based drug carriers are effective in reducing systemic toxicity and drug resistance for chemotherapy, and an emerging trend focuses on integrating cell membranes with nanomedicines for tumor targeting. Mesenchymal stem cells (MSCs) are promising candidates due to their unique tropism toward cancer cells, yet the tumor-tropic abilities can differ for MSCs sourced from different tissues. Here, a multichannel microfluidic chip to screen different sourced MSCs with the greatest tropism toward cervical cancer cells is developed. Based on this, the cell membranes from the chorionic plate-derived MSC are isolated and membrane-camouflaged platinum prodrug composite NPs for cervical cancer treatment are prepared. Results demonstrate that the composite NPs can effectively target tumor sites and have a therapeutic effect both in vitro and in vivo. It is believed that the present microfluidic platform is a powerful tool for cell screening and tumor-on-a-chip studies, and the derived nanodelivery system represents the great value of cell membrane-camouflaged nanomedicine for targeted cancer therapy.
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LUMINESCENCE
·
2022
·Vol. 37
·期 6
·980-986
·
IF 2.613
Weiwei Luo,Zhuoxin Ye,Daqian Song,Pinyi Ma
In this work, an electrochemiluminescence (ECL) sensor chip for sensitive detection of thrombin (TB) was prepared using a screen-printed electrode (SPE) as a working electrode and an aptamer as a specific recognition moiety. To produce an ECL sensor chip, a layer of p L -Cys was immobilized on the surface of the SPE using the cyclic voltammetry scanning method. A layer of gold nanoparticles (AuNPs) was assembled through an Au–S bond and hairpin DNA was further immobilized on the electrode surface. Ru(bpy) 2 (mcpbpy) 2+ , as a luminescent reagent, was covalently bound to single-stranded DNA (ssDNA) to prepare a luminescence probe ssDNA-Ru. The probe was hybridized with TB aptamer to form a capture probe. In the presence of TB, the TB aptamer in the capture probe bound to TB, causing the release of ssDNA-Ru that could bind to hairpin DNA on the electrode surface. The Ru(II) complex as a luminescent reagent was assembled onto the electrode, and p L -Cys was used as a co-reactant to enhance the ECL efficiency. The ECL signal of the sensor chip generated based on the above principles had a linear relationship with log TB concentration at the range 10 fM to1 nM, and the detection limit was 0.2 fM. Finally, TB detection using this method was verified using real blood samples. This work provides a new method using an aptamer as a foundation and SPE as a material for the detection of biological substances.
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