ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Chang Liu,Yanan Wu,Mengying Li,Fang Liu,Pengfei Kong,Haifeng Yang,Xinling Liu
Salivary d-alanine (d-Ala) and d-proline (d-Pro) are of concern for their potential in the noninvasive diagnosis of gastric cancer (GC). Most reports have succeeded in determining the total concentration of d-Ala and d-Pro. However, for personalized diagnosis and better elucidation of the underlying specific correlation of d-Ala (or d-Pro) with GC, it is desirable to determine the specific concentration of d-Ala or d-Pro. Herein, we propose an enantiomer-specific tandem assay of d-Ala based on the colorimetric reaction between 2,4-dinitrophenylhydrazine and pyruvic acid generated from the deamination of d-Ala catalyzed by d-amino acid oxidase, which is easily distinguished from l-form amino acids, d-Pro, and many other species. A linear concentration range is established from 20 to 400 μmol/L with a limit of detection of 1.01 μmol/L. Real saliva sample tests reveal that the levels of d-Ala in GC cases are remarkably higher than those in healthy individuals, which offers a simple and low-cost strategy for GC diagnosis. Simultaneously, the total concentrations of d-Ala and d-Pro in saliva are determined. Hence, the concentration of d-Pro and the proportion of d-Ala could be calculated, which further provides more molecule- and individual-specific information. This research may offer a convenient method for noninvasive diagnosis of GC and pave a new route to explore the potentials of rare d-form amino acids in disease diagnosis and treatment.
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DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 3
·441
·
IF 4.6
Shuaishuai Cui,Zhaoyang Jin,Tonglin Yu,Cunxin Guo,Yujian He,Yuhe Kan,Liang Yan,Li Wu
The accumulation of D-amino acid-containing peptides is associated with age-related diseases such as Alzheimer’s disease and cataracts, while glycosylation is an important modification of proteins and plays a key role in improving the physicochemical properties of peptides and facilitating their regulation in biological systems. This study investigates the effects of glycosylation position, glycan number, and monosaccharide structure on the conformation and enzymatic degradation of D-amino acid-containing peptides, using KYNEtWRSED (5-t) as a model peptide and six monosaccharides as model glycans. The results demonstrated that glycosylation inhibited the enzymatic degradation of 5-t in the presence of most serine-like proteases. However, in the presence of chymotrypsin, glycosylation with modified monosaccharides (except for β-D-GalNAc) promoted the degradation of 5-t. Furthermore, glycosylation had no effect on the cleavage site of 5-t. Molecular docking analysis revealed that the hydrogen bonding and electrostatic interactions between the glycopeptide and chymotrypsin were markedly strengthened, likely serving as a key determinant of the enzymatic effects. Collectively, these findings highlight the potential of glycosylation to enhance the therapeutic and biomedical applications of D-amino acid-containing peptides in disease treatment and drug design.
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DOI
PubMed
高影响因子
Applied Sciences-Basel
·
2019
·Vol. 9
·期 18
·3847
·
IF 2.217
Mingtang Tan,Peiyun Li,Wenhui Yu,Jinfeng Wang,Jing Xie
This study aimed to investigate the effects of glazing with sodium polyacrylate (SP) and D-sodium erythorbate (DSE) on the quality changes of squid during frozen storage. Frozen squid samples were randomly divided into seven groups: (1) CK (unglazed); (2) WG (distilled water-glazed); (3) SG (0.1% SP -glazed); (4) SG-1DSE (0.1% SP with 0.1% DSE -glazed); (5) SG-3DSE (0.1% SP with 0.3% DSE-glazed); (6) SG-5DSE (0.1% SP with 0.5% DSE-glazed); (7) WG-1DSE (0.1% DSE-glazed). The efficacy of the different coatings was evaluated using various indicators, such as water holding capacity (WHC), pH value, low field nuclear magnetic resonance (LF-NMR), color, malondialdehyde (MDA) content value, free amino acids (FAAs) content, intrinsic fluorescence intensity (IFI) and the total sulfhydryl content (SH) content. Intrinsic fluorescence intensity (IFI) and low field nuclear magnetic resonance (LF-NMR) were used as fast monitoring techniques to monitor changes in quality of squid samples. The results showed that compared with the CK and WG groups, coating with either SG or DSE alone resulted in reduced rate of moisture loss (p< 0.05), lipid oxidation (p< 0.05) protein degradation (p< 0.05) and prolonged its shelf-life. The combination of glazing treatment with SG and DSE (groups SG-1DSE, SG-3DSE and SG-5DSE) further improved the protective effects of coating, particularly in the SG-3DSE group. Therefore, the glazing of SG-3DSE is recommended to be used to control the quality of frozen squid and to prolong its shelf-life during frozen storage.Keywords:squid;glazing;frozen storage;sodium polyacrylate;D-sodium erythorbate;quality
研究领域:
食品科学
·
海洋生物学
·
保鲜技术
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DOI
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2020
·Vol. 31
·期 6
·1216-1224
·
IF 1.399
Han Qing,Wei Lanlan,Deng Guozhe,Sun Ying,Deng Jianjun,Tang Zigang,Fan Jinhui,Wang Xia,Kang Xuejun
The detection of vitamin D in complex samples is challenging because of the instability of vitamin D in sample preparation. To resolve this problem, packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D2 (D2) and vitamin D3 (D3) in dairy products. Based on PFSPE, vitamin D could avoid oxidation because of the shorter extraction time. The adsorbent materials, polystyrene/polypyrrole (PS/PPy) nanofibers, were prepared by electrospinning of PS and in situ polymerization of PPy on the surface of PS nanofibers. The linearity was within the range of 1-2000 ng mL−1 in dairy solution, and the recoveries were 91.9-109.1% with a relative standard deviation (RSD) < 13.6%. Therefore, the prepared method could be used for the detection of D2 and D3 in dairy products.
研究领域:
分析化学
·
食品科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF THERMOPHYSICS
·
2020
·Vol. 41
·期 5
·1-12
·
IF 0.794
Mo Songping,He Lijuan,Jia Lisi,Chen Ying,Cheng Zhengdong
Thermophysical properties are important parameters that influence the performance of phase change materials (PCMs) and heat transfer fluids. Micro/nanoencapsulation is an effective technique for preventing leakage and enhancing thermophysical properties of PCMs. In the present study, novel d -mannitol nanocapsules with inorganic shells were synthesized as a medium-temperature PCM. The nanocapsulation was confirmed by scanning electronic microscopy. The specific heat and thermal diffusivity of the nanocapsules and their suspension with heat transfer oil as base fluid were measured by a differential scanning calorimeter and light flash apparatus. The thermal conductivity of the nanocapsule suspension was measured using a hot-disk thermal constants analyzer. The viscosity of the nanocapsule suspension was measured by a rotational rheometer. The results show that the thermal conductivity and thermal diffusivity of the nanocapsules were enhanced compared to the bulk of the PCM due to the inorganic shell. The specific heat, thermal conductivity, and viscosity of the nanocapsule suspension were increased compared to the base fluid. The potential of the nanocapsule suspension as a heat storage and transfer fluid was evaluated.
研究领域:
材料科学
·
热力学
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DOI
高影响因子
Applied Chemistry for Engineering
·
2025
·Vol. 36
·期 1
·36-42
·
IF 0.6
Gyu Won Woo,Eun Kwang Lee
본 연구에서는 스크린 인쇄 기술을 활용하여 유연 기판인 polyimide (PI) 필름에 내부 이온 게이트 유기 전기 화학 트랜지스터(internal gated transistor, IGT)를 제작하였다. 기존의 복잡한 공정 없이 저비용으로 제작하였으며, 유기 전기 화학 트랜지스터(OECT)의 채널로 주로 사용되는 전도성 고분자인 poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate) (PEDOT:PSS)와 D-소르비톨을 혼합하여 외부 전해질 없이 채널 내에서 자체적으로 이온 (de)doping 메커니즘을 구현하였다. 그 결과, 스크린 인쇄 IGT는 기존 OECT 대비 향상된 전도성과 성능 지표를 나타냈다. D-소르비톨 첨가 후 PEDOT:PSS의 시트 저항 값이 기존 대비 약 1/2로 감소하여 전도성이 향상되었고, 표면 결정성 또한 개선되었다. 전기적 성능 평가에서 IGT는 OECT 대비 출력 곡선 특성(VGS = 1.6 V)에서 약 4배 증가한 것으로 나타났으며, ION/IOFF 비율은 약 4.3배 높았다. 이동도 계산 결과 OECT는 0.6987 F V-1 s-1 cm-1, IGT는 0.9578 F V-1 s-1 cm-1로 IGT가 높은 값을 보였고, 시간 응답 속도 상수 τ는 τOECT = 0.50 s, τIGT = 0.34 s로 더 빠른 응답 속도를 나타내었다.
The device fabricated in this study was an internal ion-gated organic electrochemical transistors (IGTs) using screen printing technology on a flexible polyimide (PI) film substrate. This fabrication method is a cost-effective and simple method without existing deposition techniques or complicated processes. The conductive polymer, poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate) (PEDOT:PSS), commonly used as a channel material in organic electrochemical transistors (OECTs), was blended with D-sorbitol to enable a self-ion (de)doping mechanism within the channel, eliminating the requirement for an external electrolyte. The screen-printed IGT exhibited enhanced conductivity and performance compared to conventional OECTs. Analysis of the physical and chemical properties of PEDOT:PSS with D-sorbitol revealed a significant reduction in sheet resistance. The surface analysis further confirmed an enhancement in crystallinity. Electrical performance evaluation involved a comparative study with conventional OECTs. The IGT device demonstrated a 4 times increase in output curve characteristics at VGS = 1.6 V compared to OECTs. Additionally, the ION/IOFF ratio was 4.3 times higher for the IGT. Calculations of µC* to assess device mobility indicated a notable improvement, with values of 0.9578 F V-1 s-1 cm-1 for IGT compared to 0.6987 F V-1 s-1 cm-1 for OECTs. In time constant analysis, τOECT = 0.50 s and τIGT = 0.34 s indicate that IGTs have a faster response time.
研究领域:
材料科学
·
电子工程
·
化学工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Pengfei Shan,Yingwen Lu,Weilin Lu,Xiangping Yin,Haiwei Liu,Daai Li,Xiaoyue Lian,Weiping Wang,Zhongyu Li,Zhihui Li
The low utilization efficiency of pesticides exerts an adverse impact on the environment and human health. Polymer-related controlled-release nanosized pesticide systems provide a promising and efficient way to overcome the problem. In this work, a biodegradable and light-responsive amphiphilic polymer was synthesized via 1,1,3,3-tetramethylguanidine-promoted polyesterification under mild conditions (low temperature, no vacuum, and no inert gas protection). We used this polymer to fabricate a light-triggered controlled-release nanosized pesticide system. The herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D), was selected as a model drug to show its potential as a controlled-release pesticide system. It was found that the 2,4-D-loaded polymeric nanoparticles were stable without the treatment of UV, while the release rate of 2,4-D from the nanoparticles gradually increased after treatment with UV light. Pot trial showed that the 2,4-D-loaded polymer nanoparticles showed a good herbicidal effect. Finally, toxicity studies suggested that the polymer can reduce toxicity to nontarget organisms.
研究领域:
环境科学
·
材料科学
·
农业科学
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DOI
高影响因子
Solar RRL
·
2025
·
IF 4.7
Xinyu Zhao,Songlin Lu,Keyu Cui,Ye Zhang,Jing Luo,Chunbo Liu,Huaqiao Tan
The exciton binding energy ( E b ) is recognized as a pivotal parameter governing charge separation in polymeric photocatalysts. Reducing E b in polymers enhances carrier generation, thereby boosting photocatalytic activity. Rationally constructing donor–acceptor (D–A) architectures in heptazine-based polymers offers an effective strategy to overcome intrinsic excitonic limitations. In this work, CN x ( x = 0.015, 0.025, 0.05) materials with intramolecular D–A configurations were developed by integrating electron-donating units with heptazine acceptor motifs. The optimized material CN 0.025 exhibits a 29% reduction in E b (97 → 69 meV) and extended visible-light absorption, attributable to enhanced π-conjugation and modulated electronic structures. Remarkably, under visible-light irradiation, CN 0.025 achieves 97.7% degradation of 2,4-dichlorophenol (10 ppm) within 50 min. Moreover, CN x exhibits a 29.8-fold higher CO yield than pure g-C 3 N 4 in photocatalytic CO 2 reduction. Electrochemical photocurrent measurements reveal a remarkable enhancement in the photocurrent response of CN 0.025 relative to g-C 3 N 4 , which suggests more efficient exciton dissociation and increased carrier availability at the catalytic surface for subsequent redox reactions. This work establishes a paradigm for exciton dissociation in polymeric photocatalysts through engineered D–A nanoarchitectures.
研究领域:
材料科学
·
光催化
·
聚合物化学
·
环境修复
·
能量转换
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产品关联
DOI
高影响因子
BIOLOGICAL & PHARMACEUTICAL BULLETIN
·
2025
·Vol. 48
·期 12
·1897-1905
·
IF 1.7
Xiaojun Zhang,Fang Wang,Yuna Dai,Zhaoyu Gao,Jianchao He
Platycodon grandiflorum (P. grandiflorum), a traditional Chinese herbal medicine, possesses various biological activities. Among its constituents, polygalacin D (PGD) stands out as one of the principal compounds. This study determined the regulatory effect of PGD on breast cancer progression. Breast cancer cells MCF-7 and BT474 were treated with different concentrations of PGD. Colony formation assays, the detection of Ki67 expression, and cell cycle analysis were performed to verify the effect of PGD on the proliferation of breast cancer cells. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining, flow cytometry, and examination of caspase-3 activity were conducted to assess the changes in apoptosis following PGD treatment. In addition, the co-treatment of PGD and cisplatin (DDP) on cells was performed to explore the effect of PGD on the chemosensitivity of DDP. Furthermore, bioinformation analysis suggested that histone deacetylase 1 (HDAC1) and HDAC2 were the downstream factors of PGD, and the effect of PGD on their expression was assessed. PGD inhibited breast cancer cell proliferation, as evidenced by reduced colony formation, downregulation of Ki67 expression, and induction of cell cycle arrest. Additionally, PGD treatment significantly increased caspase-3 activity and elevated the rate of cell apoptosis and TUNEL-positive cells. Furthermore, PGD was found to enhance the chemosensitivity to DDP. Mechanistically, PGD reduced the expression of HDAC1 and HDAC2 by promoting their protein degradation. Cell proliferation induced by HDAC1 or HDAC2 was also overcome by PGD. In conclusion, PGD was confirmed as an effective tumor inhibitor that suppressed the development of breast cancer by regulating HDAC1 and HDAC2.
研究领域:
肿瘤学
·
分子生物学
·
药理学
·
天然产物化学
·
表观遗传学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2011
·Vol. 21
·期 44
·17972-17977
·
IF 5.101
Pengtao Shao,Hongzhi Wang,Qinghong Zhang,Yaogang Li
Mn-doped ZnSe nanocrystals (Mn:ZnSe d-dots) that emit white light were synthesized in aqueous media in microfluidic reactors. White light emission from Mn:ZnSe d-dots was a result of combined emission of blue light (emission from non-coordinated surface selenium sites) and yellow light (4T1 → 6A1 emission from Mn2+ ions). Emission from non-coordinated surface selenium sites was realized by adjusting the ratio of the flow volumes of [Zn]/[Mn] and the mass of stabilizer added. Photoluminescence from the Mn:ZnSe d-dots was easily tuned from 580 to 602 nm by regulating flow volume. Under the optimal experimental conditions, Mn:ZnSe d-dots that emit white light with a quantum yield of about 10.2% were fabricated.
研究领域:
材料科学
·
纳米技术
·
光学
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DOI
高影响因子
Journal of Macromolecular Science Part B-Physics
·
2017
·
IF 0.828
Shan Bai,Zhenzhong Ouyang
Direct graft copolymerization of maleic anhydride (MAH) onto poly(D,L-lactide-co-glycolide) (PLGA) was performed by use of benzoyl peroxide (BPO) as initiator. Fourier transform infrared spectra, nuclear magnetic resonance, titration analysis of maleation extent and water contact angle were employed to characterize the obtained MPLGA. The effects of monomer concentration, initiator concentration and reaction temperature on the degree of maleation were investigated. The MPLGA scaffold was obtained by using NaCl as the porogen. The results showed that maleic anhydride was successfully grafted onto the PLGA chains. The monomer and initiator concentrations played an important role in the graft copolymerization; the extent of maleation first increased with increasing content of MAH and BPO and then declined. The extent of maleation continuously increased with increasing temperature to 100°C, and then the rate of increase decreased. The extent of maleation could be controlled in the range from 0.15 to 0.71% by adjusting the graft copolymerization conditions. The introduction of anhydride enhanced the water contact angle of PLGA. The MPLGA scaffold showed a network of interconnected pores ranging from 50 to 300 μm.
研究领域:
高分子化学
·
生物材料科学
·
高分子改性
·
组织工程
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DOI
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2012
·
IF 1.547
Li Zhang,Xiu-Ling Wu,An-Qi Wang,Fei Qin,Da-Wei Meng,Xiao-Yang Liu
A manganese(II) coordination polymer [Mn(TMB)2 · H2O]
n
(1) (HTMB = 3,4,5-trimethoxybenzoic acid) has been synthesized under hydrothermal conditions and characterized by single-crystal X-ray diffraction, elemental analysis, powder X-ray diffraction analysis, spectroscopic (IR, solid state UV-Vis), and thermal methods. The crystal belongs to orthorhombic system, space group P212121, with cell parameters a = 7.3001(8), b = 11.4146(13), c = 27.053(3) Å, α = β = γ = 90°, V = 2254.3(4) Å3, Z = 4. In 1, TMB in two different coordination modes bridges six-coordinate manganese(II) centers forming a 1-D infinite chain coordination framework. The spectral and thermal properties of the complexes have also been studied.
研究领域:
无机化学
·
配位化学
·
材料科学
·
晶体工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）