SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Wenfeng Zhao,Mimi Sun,Tian Lei,Xiaojun Liu,Qingquan Zhang,Chenghua Zong
In this paper, we present a novel, rapid and sensitive sensor for biothiols including detection of glutathione (GSH), cysteine (Cys) and homocysteine (Hcy) based on an indicator displacement assay (IDA) for turn on the fluorescence of Murexide-Hg 2+ system. In this IDA approach, a commercially available dye, murexide (Mu), using as the indicator and selective detection of biothiols was achieved based on the competition between the indicator and biothiols for the binding with Hg 2+ . The fluorescence of Mu could be quenched in the presence of Hg 2+ due to the coordination between the indicator and Hg 2+ . Subsequently, the fluorescence of the Mu-Hg 2+ system was recovered gradually with the addition of biothiols such as GSH, Cys or Hcy due to their stronger affinity with Hg 2+ . A good relationship was obtained from 0.1–40 μM for GSH, from 0.5–30 μM for Cys and from 0.5–50 μM for Hcy, respectively. This method has been successfully applied to the trace detection of GSH, Cys and Hcy in human urine and serum samples with satisfactory results. Remarkably, the sensing process is able to be observed by the naked eye under UV irradiation which provides an instrument-free platform for point-of-care monitoring.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Tao Zheng,Zhenxiang Xu,Yong Zhao,Hongqi Li,Rong Jian,Congcong Lu
A spiropyran-appended polysiloxane ( SP-Si ) was synthesized and characterized. The photochromic SP-Si has good thermal stability and exhibits high fatigue resistance to light. SP-Si acts as a dual colorimetric and spectroscopic probe for determining multi-scale pH over a wide range of 1∼14, and for detection of Ag + and Fe 3+ ions. Based on the transformation from colorless SP-Si solution without absorption at 440 nm to brown SP-Si /Ag + showing extremely strong absorption at 440 nm by addition of Ag + ions and subsequent transformation from brown to colorless SP-Si /Ag + /Hg 2+ with very weak absorption at 440 nm after addition of 1 equivalent of Hg 2+ ions, SP-Si /Ag + system serves as a dual colorimetric and spectroscopic probe for highly selective and sensitive detection of Hg 2+ ions. The detection is not interfered by common competitive metal ions such as Na + , K + , Li + , Ni 2+ , Fe 2+ , Zn 2+ , Co 2+ , Sr 2+ , Cu 2+ , Al 3+ , Ce 3+ and Cr 3+ . Applicability of SP-Si for real water sample analysis has been proved.
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高影响因子
LUMINESCENCE
·
2015
·Vol. 30
·期 8
·1263-1268
·
IF 1.518
Tong-Mou Geng,Da-Yu Wu
We report the fabrication of a novel easily available turn-on fluorescent water-soluble polymeric chemosensor for Hg 2+ ions that was simply prepared by micellar free radical polymerization of a water-insoluble organic rhodamine-based Hg 2+ -recognizing monomer (GR6GH), with hydrophilic monomers acrylamide (AM) and acrylic acid (AA). The chemical structure of the polymeric sensor was characterized by FT-IR and 1 H NMR spectroscopy. The apparent viscosity average molecular weight M η of poly(acrylamide–acrylic acid) [poly(AM–NaAA)] and the water-soluble polymeric chemosensor poly(AM–NaAA–GR6GH) were 1.76 × 10 6 and 6.84 × 10 4 g/mol, respectively. Because of its amphiphilic property, the water-soluble polymeric chemosensor can be used as a chemosensor in aqueous media. Upon addition of Hg 2+ ions to an aqueous solution of poly(AM–NaAA–GR6GH), fluorescence enhancements were observed instantly. Moreover, other metal ions did not induce obvious changes to the fluorescence spectra. This approach may provide an easily measurable and inherently sensitive method for Hg 2+ ion detection in environmental and biological applications. Copyright © 2015 John Wiley & Sons, Ltd.
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高影响因子
Journal of Materials Chemistry A
·
2015
·Vol. 3
·期 21
·11587-11595
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IF 7.443
Lijin Huang,Man He,Beibei Chen,Bin Hu
A magnetic MOF composite was successfully prepared by a novel and green strategy through reasonable design. The nano Fe3O4@SiO2 core was first coated by a shell of Cu(OH)2 as the self-template, followed by a conversion of Cu(OH)2 into HKUST-1 at room temperature in a water–ethanol mixture solvent, and Fe3O4@SiO2@HKUST-1 core–shell nanostructures were obtained successfully. Based on this, a Bi-I-functionalized-magnetic HKUST-1 composite was prepared through a flexible coordination-based post-synthetic strategy. Powder X-ray diffraction spectrometry, N2 sorption–desorption isotherms and Fourier transform infrared spectroscopy were used for the characterization of the magnetic MOF composites. The core–shell morphology was confirmed by transmission electron microscope images and the result of thermogravimetric analysis showed that the magnetic composites had excellent thermal stability. Besides, the adsorption of Hg2+ on Fe3O4@SiO2, Fe3O4@SiO2@HKUST-1 and Bi-I-functionalized magnetic MOF composites was investigated, and a good adsorption selectivity of Bi-I-functionalized magnetic MOF composites towards Hg2+ was demonstrated, with high adsorption capacity (264 mg g−1) and fast adsorption dynamics. The Bi-I-functionalized magnetic HKUST-1 composite was then successfully employed for the selective removal of Hg2+ from water, demonstrating great potential application of the prepared magnetic MOF composite as a fascinating adsorbent in environmental monitoring.
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高影响因子
JOURNAL OF POLYMER RESEARCH
·
2015
·Vol. 22
·期 3
·1-8
·
IF 1.92
Geng Tong-Mou,Wu Da-Yu,Huang Wei
An acrylic monomer bearing rhodamine 6G group, salicylaldehyde rhodamine 6G hydrazone acrylate (AR6GHS), was synthesized from salicylaldehyde rhodamine 6G hydrazone (R6GHS) and acryloyl chloride in the presence of triethylamine in dry dichloromethane (CH 2 Cl 2 ) at room temperature. The poly(acrylamide- salicylaldehyde rhodamine 6G hydrazone acrylate) (poly(AM-AR6GHS)) was synthesized by a combiation of AR6GHS and a hydrophilic comonomer acrylamide (AM) with 2,2′-azobis(2-methylpropionitrile) (AIBN) as a thermal initiator in tetrahydrofuran (THF). The resultant AR6GHS and poly(AM-AR6GHS) were identified by FTIR and 1 H NMR spectra. The fluorescent characteristic of the poly(AM-AR6GHS) in the aqueous solutions were investigated both in varied pH and in the presence of metal cations. It was found that the copolymeric sensor exhibited “off-on” recognition toward Cu 2+ and Hg 2+ over a wide range of tested metal ions with remarkably enhanced fluorescent intensities. The poly(AM-AR6GHS) had a good linear response between the relative fluorescence intensity and Cu 2+ or Hg 2+ concentration. In addition, upon the addition of Cu 2+ or Hg 2+ into the aqueous solution of poly(AM-AR6GHS), the color changes occurred from colorless to pale pink or pink under visible light, and the emission color of the sensor solution turned from dark to bright greenish yellow or orange yellow under the UV light at 365 nm.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yang Li,Jing Li,Ting Yu,Hongyi Cai,Youyu Zhang,Haitao Li,Huijun Zhou,Peng Yin
The Mercury (II) ion (Hg²⁺) is a toxic heavy metal that threatens biological systems by inducing oxidative stress and disrupting the redox balance. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are critical in maintaining redox homeostasis and are implicated in numerous physiological and pathological processes. Understanding the complex interactions between Hg²⁺ and biothiols requires molecular tools capable of simultaneous detection. Herein, we report a novel multi-signal fluorescent probe, DPC , designed with four specific recognition sites and rhodamine and coumarin fluorophores. The DPC probe enables the selective and sensitive differentiation of Mercury (II) ion (Hg²⁺), cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), with distinct fluorescence signals. DPC can detect mercury (II) ions (Hg²⁺) in water samples with a high recovery rate ranging from 90.44 % to 112.27 %. DPC was also successfully applied for real-time imaging of these species in living cells and zebrafish, revealing that Hg²⁺ induces oxidative stress, reducing cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) levels. Furthermore, detoxifying agents such as sodium selenite (Na₂SeO₃), 2,3-dimercaptopropan-1-ol (DMPC), and L-selenocysteine (Sec) restored biothiol levels, counteracting Mercury (II) ion (Hg²⁺) toxicity. These results highlight the exceptional performance of DPC for the simultaneous imaging of Mercury (II) ions (Hg²⁺) and biothiols, providing insights into their interactions and proposing a chemobiological framework for studying heavy metal toxicity and developing detoxification strategies.
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Huifang Wu,Qiqi Wang,Miaochen Dong,Xinyue Liu,Yanfeng Tang
Accurate and rapid monitoring of organophosphorus pesticides (OPs) residues is crucial for regulating food safety. Herein, dual-emission carbon dots (de-CDs) were fabricated for the ratiometric detection of OPs and Hg 2+ . The de-CDs exhibited two emission peaks at 678 and 485 nm when excited with visible light. Interestingly, the fluorescence at 678 nm was significantly quenched by Hg 2+ mainly because of the static quenching effect, whereas that at 485 nm exhibited a slight change. More significantly, the quenched fluorescence of the de-CDs recovered remarkably after introducing omethoate , diazinon and malathion . Accordingly, the ratiometric detection of the three OPs and Hg 2+ was achieved with high selectivity and robust performance. In addition, the OPs residues assay in Brassica chinensis was successfully performed with satisfactory results. This study not only provides an attractive tool for the simple and rapid assay of OPs but also offers new insights into the fabrication of multi-functional carbon dots .
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高影响因子
RSC Advances
·
2012
·Vol. 3
·期 7
·2227-2233
·
IF 0
Zhengping Dong,Xin Tian,Yuanzhe Chen,Jingran Hou,Jiantai Ma
Rhodamine group modified SBA-15 nanocomposite (R3-SBA-15) is investigated as a highly selective Hg2+ sensor. The colorless sensor converts to red color in the presence of Hg2+ with the fluorescent detection limit for Hg2+ in MeCN–H2O solution (9 : 1 v/v) being 15 ppb levels and exhibits excellent Hg2+-specific luminescence enhancement over various competitive cations, including alkali and earth, the first-row transition metals and heavy metals. According to the definition of Boolean Logic, using Hg2+ and S2− as inputs, the fluorescence intensity signal changes of R3-SBA-15 as outputs, R3-SBA-15 can act as an INHIBIT logic gate. All the results suggest that R3-SBA-15 was possible for real-time quantitative detection of Hg2+ and the convenience for potential application as a logic gate operated at the nanoscale level.
研究领域:
分析化学
·
纳米科学与技术
·
环境监测
·
生物传感
·
分子探针
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RSC Advances
·
2026
·Vol. 16
·期 3
·2652
·
IF 4.6
Yongjian Pan,Shancai Guo,Teng Wang,Jianyuan Lin,Zhijian Jia,Fuhang Xu,Renyue Huang,Haili Wang,Chunjie Bai,Jianghua Chen
Nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs) were synthesized via a green, one-step hydrothermal method using waste lemon peel as the carbon source and glutathione (GSH) as the N,S dopant in a dimethylformamide (DMF) medium. The reaction was conducted at 180 °C for 10 hours to achieve efficient synthesis. The resulting N,S-CQDs showed excellent water dispersibility, strong photostability, and bright fluorescence, with optimal excitation and emission wavelengths of 340 nm and 420 nm, respectively. High-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) confirmed their uniform morphology and successful incorporation of nitrogen and sulfur heteroatoms. Notably, the fluorescence of the N,S-CQDs could be rapidly and selectively quenched by Hg 2+ within 10 minutes at room temperature, without requiring any additional surface modification of the CQDs or auxiliary reagents in the sensing procedure. Under optimized conditions, the sensor exhibited a linear fluorescence quenching response to Hg 2+ over the concentration range of 17.6 nM to 20 µM ( R 2 = 0.9899), with a low detection limit of 17.6 nM. Mechanistic studies suggested that the quenching primarily resulted from the formation of nonfluorescent complexes between Hg 2+ and functional groups on the CQD surface. The developed sensor was successfully applied to detect trace levels of Hg 2+ in real lake water samples collected near textile manufacturing sites, demonstrating its potential as a sustainable, rapid, and cost-effective tool for practical environmental mercury monitoring. Nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs) were synthesized via a green, one-step hydrothermal method using waste lemon peel as the carbon source and glutathione (GSH) as the N,S dopant in a dimethylformamide (DMF) medium.
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高影响因子
Food Analytical Methods
·
2026
·Vol. 19
·期 10
·277
·
IF 3
Kang Ling,Cui Qi-Qi,Chen Jing-Jing,Shi Xiao-Bei,Yan Hui,Ge Li-Lin,Wang Tian-Shu,Zhou Gui-Sheng
Severe threats to food safety and human health are posed by Hg 2 ⁺, thereby necessitating the development of rapid, sensitive, and portable on-site detection methods. In the present study, the aspartic acid-functionalized gold nanoparticles (Asp-AuNPs) were prepared via the one-step green synthesis strategy. The structural characteristics of Asp-AuNPs and their Hg 2 ⁺-sensing properties were systematically characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared (FT-IR) spectroscopy, and zeta potential measurements. The Asp-AuNPs-based colorimetric sensing platform was developed for the detection of Hg 2 ⁺, achieving a limit of detection (LOD) of 2.6 nM and a linear detection range from 0.005 to 1.0 µM. Concurrently, a smartphone-assisted color feature analysis platform was constructed, which exhibited two distinct linear segments with high correlation coefficients for the quantitative assay of Hg 2 ⁺. When applied to real samples, including pears, grapes, potatoes, and Chinese medicinal herbs ( Selaginella and Corydalis yanhusuo ), this smartphone-integrated system afforded satisfactory recoveries ranging from 90.39 to 109.16%, with relative standard deviations of less than 6.15%. Notably, the analytical results obtained via the smartphone-based platform were found to be in excellent agreement with those derived from the Asp-AuNPs colorimetric method. The principal component analysis (PCA) and t-distributed Stochastic Neighbor Embedding (t-SNE) were applied for machine learning analysis of colorimetric images. After reducing the high‑dimensional color features with t‑SNE, a k‑nearest neighbors (k‑NN) classifier was used to recognize different Hg 2 ⁺ concentration levels, achieving an accuracy of 91.41% with favorable repeatability. The fabricated Asp-AuNPs-based sensing system exhibited excellent sensitivity, selectivity and portability, thereby providing a reliable technical strategy for the on-site detection of Hg 2 ⁺ in food and herbal matrices. Graphical
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高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 3
·122528
·
IF 7.5
Yu Gao,Linan Zhou,Qingqing Peng,Tingting Cui,Ziyi Guan,Fang Zhang,Li Wang,Huiyi Yang,Fang Chai
Multiple trace heavy metal pollutants present a serious threat to both the environment and ecosystems, and their coexistence also affected the detection efficiency. Therefore, multi-mode responsive optical probes with versatile application prospects for optical sensing are highly desired. Here, yellow-emissive carbon dots (Y-CDs) with aggregation-induced emission (AIE) characteristics were fabricated via a hydrothermal approach, boasting a absolute quantum yield of 13.88% and a fluorescence lifetime of 8.55 μs. Leveraging the AIE behavior and quenching effect, Y-CDs were successfully achieved the multivariate detection of Ag + , Hg 2+ separately, and further realized cascade sensing BSA, with detection limits of 5.59 μM, 7.7 nM, and 0.9 μM respectively. Subsequently, a portable smartphone-based sensing platform was developed to facilitate rapid on-site analysis, and a logic gate was designed to expedite integration into intelligent devices. Furthermore, Y-CDs demonstrated remarkable antibacterial action via in vitro antibacterial assays, displaying significant inhibitory effects against both E. coli and S. aureus , providing the possibilities for testing in the food and medical fields with high standards of hygiene permits. With their exceptional multifunctional sensing capabilities, Y-CDs were expected holding extensive promising application prospects in the realm of real-time monitoring of environmental and food safety.
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高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Meiling Long,Xinyuan Wang,Shaogui Wu
In this study, sulfur and nitrogen co-doped fluorescent carbon dots (S,N-CDs) were synthesized via a hydrothermal method using Paliurus ramosissimus as a natural and sustainable carbon source. The as-prepared S,N-CDs exhibited a high quantum yield of 73.3% in ethanol, along with pronounced solvent-dependent and excitation-dependent fluorescence properties. Fluorescence sensing investigations demonstrated that the S,N-CDs possessed excellent selectivity toward erythromycin (EM) and Hg 2+ , with limits of detection of 0.27 μM and 38 nM, respectively. The quenching process was attributed to a static quenching mechanism, and satisfactory anti-interference performance was observed. Furthermore, a smartphone-assisted visual detection strategy was established based on the distinct fluorescence color variation of S,N-CDs in response to different EM concentrations, achieving a detection limit as low as 0.41 μM. Spike-and-recovery experiments revealed recoveries of 87.97%–95.52% in rainwater samples and 100.88%–107.11% in milk samples, with relative standard deviations (RSDs) below 3%, indicating favorable practical applicability. Overall, the developed S,N-CDs fluorescent probe combines high sensitivity, strong selectivity, and environmental friendliness, providing a promising platform for the rapid detection of multiple contaminants in environmental and food matrices and broadening the application scope of plant-derived carbon dots.
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高影响因子
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