CRYSTAL GROWTH & DESIGN
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2024
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IF 3.2
Shuhong Song,Lei Wang,Yaqian Qu,Huimin Li,Hongshuai Wang,Peizhuo Han,Zeliang Gao,Xutang Tao
The crystal growth mechanism of form I (AZ-I) of the antihypertensive drug azilsartan is revealed by in situ atomic force microscopy (AFM). On the dominant (100) face, AZ-I grows by a classical spiral growth mechanism. The growth spiral is asymmetric due to the different step kinetics in different crystallographic directions. The normal growth rate of the (100) face is inferred to be much lower than the step velocity within this face according to the step geometry, resulting in the plate-like crystal morphology. The dependence of step velocity on terrace width indicates that molecules incorporate into steps primarily by a surface diffusion mechanism involving molecular adsorption on terraces at first and then diffusion into steps. Within the (100) face, steps in different directions exhibit different step edge free energies and total minimal energies for step advancement. The step kinetics depends on the step length within a certain range. These findings provide a fundamental understanding of drug crystal growth at the microscale.
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Cell Discovery
·
2025
·Vol. 11
·期 1
·1-14
·
IF 12.5
Wang Jinrui,Niu Shenghui,Hu Xiao,Li Tianxing,Liu Shengduo,Tu Yingfeng,Shang Zehua,Zhao Lin,Xu Pinglong,Lin Jingwen,Chen Lu,Billadeau Daniel D.,Jia Da
The cGAS-STING pathway mediates the innate immune response to cytosolic DNA, contributing to surveillance against microbial invasion or cellular damage. Once activated, STING recruits TBK1 at the trans-Golgi network (TGN), which in turn phosphorylates IRF3 to induce type I interferon (IFN-I) expression. In contrast to STING, little is known about how TBK1 is transported to the TGN for activation. Here, we show that multiple TGN tethering factors, a group of proteins involved in vesicle capturing, are indispensable for STING-IFN-I signaling. Deletion of TBC1D23, a recently reported tethering factor, in mice impairs the STING-IFN-I signaling, but with insignificant effect on STING-NF-κB signaling. Mechanistically, TBC1D23 interacts with TBK1 via the WASH complex subunit FAM21 and promotes its endosome-to-TGN translocation. Furthermore, multiple TGN tethering factors were reduced in aged mice and senescent fibroblasts. In summary, our study uncovers that TGN tethering factors are key regulators of the STING-IFN-I signaling and suggests that their reduction in senescence may produce aberrant STING signaling.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Yuhua Zhen,Xinyu Cai,Wenjie Yang,Yuanyue Qie,Jiahao Li,Gaohui Ren,Wei Xing,Qingzhong Xue,Hongxia Geng
Sulfide solid electrolytes (SSEs) are promising materials for developing safe and high-performance all-solid-state lithium batteries (ASSLBs). However, enhancing the ionic conductivity, air stability, and electrolyte/electrode interface compatibility of vulcanized solid electrolytes simultaneously remains a significant challenge. Here, we propose a dual doping strategy (In 2 S 3 and LiI) based on liquid-phase method for the preparation of SSEs. In 2 S 3 and LiI can widen the Li + diffusion pathways and enhance the ionic conductivity. Meanwhile, In 2 S 3 can enhance the (electro)chemical stability and suppress the formation of lithium dendrites and H 2 S. Consequently, the prepared Li 7 P 2.94 In 0.1 S 10.9375 I 0.125 (LPInSI) solid electrolyte exhibited high ionic conductivity (2 mS·cm −1 ), excellent electrochemical stability, and air stability at 25 ℃. Based on this, ASSLBs fabricated with Li-In alloy anodes enhance the compatibility of the electrolyte/electrode interface, deliver a discharge capacity of 165.2 mAh·g −1 at room temperature, and retain 87.6 % of the specific discharge capacity after 300 cycles at 0.2 C. This study offers a viable approach for the synthesis of practical SSEs and high-performance ASSLBs.
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Frontiers in Pharmacology
·
2020
·
IF 4.225
Zhang Jiaqi,Liang Xiaoqiang,Li Jiacheng,Yin Hao,Liu Fangchen,Hu Cheng,Li Ling
Overuse of acetaminophen (APAP) is a major cause of drug-induced liver failure at the clinics. Apigenin (API) is a natural flavonoid derived from Matricaria chamomilla. The aim of the present study was to investigate the amelioration function of API in APAP-induced hepatotoxicity both in vitro and in vivo and investigate its potential mechanisms. Analysis results of the activities of serum alanine and aspartate aminotransferases (ALT and AST), malondialdehyde, myeloperoxidase (MPO), and reactive oxygen species (ROS) demonstrated therapeutic effects of API. MTT assay results revealed that API attenuated APAP and its metabolic product, N-acetyl-p-benzoquinone imine (NAPQI) induced cytotoxicity in a dose-dependent manner in human liver cells, L-02 cells. Subsequently, metabolomic results of cells and serum analyses demonstrated an aberrant level of carnitine palmitoyltransferase I (CPT1A). We established that API stimulated CPT1A activity in mice liver tissues and L-02 cells. Molecular docking analyses revealed potential interaction of API with CPT1A. Further investigation of the role of CPT1A in L0-2 cells revealed that API reversed cytotoxicity via the AMP-activated protein kinase (AMPK)/GSK-3β signaling pathway and compound C, which is a selective AMPK inhibitor, inhibited activation of CPT1A induced by API. API was bound to the catalytic region of AMPK as indicated by molecular docking results. In addition, compound C suppressed nuclear translocation of nuclear factor erythroid 2–related factor 2 (NRF2) that is enhanced by API and inhibited the antioxidative function of API. In summary, the study demonstrates that API attenuates APAP-induced hepatotoxicity by activating the AMPK/GSK-3β signaling pathway, which subsequently promotes CPT1A activity and activates the NRF2 antioxidant pathway.
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Advances in Experimental Medicine and Biology
·
2016
·
IF 1.953
Yan Heping,Chen Yashun,Zhang Jucheng,Liu Wei,Chen Rui
The first derivatives of gelatin and type I collagen fluorescence spectra were characterized in order to describe the effect of free radicals on pyridinoline (PYD) cross-links. The different gas saturation conditions were used to investigate the effect of different free radicals. An analysis of first derivative fluorescence spectra suggests that PYD cross-link fluorescence emission is composed of three peaks in gelatin, but only two in type I collagen. The PYD cross-link was photo-degraded more than other gases in the presence of O 2 . This suggests that the singlet oxygen ( 1 O 2 ) plays a key role when using photodynamic therapy to treat skin fibrosis disease with Hypocrellin B (HB).
研究领域:
光生物学
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皮肤科学
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分子生物学
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结缔组织病
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自由基化学
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光动力学
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纤维化疾病
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JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2025
·
IF 2.7
Yun Li,Ruijin Pu,Meng Su,Ruiqin Mao,Yajun Zheng,Zhiping Zhang
Methimazole (MMI), which is primarily known as an antithyroid drug, has received considerable attention for its potent antioxidant properties in a copper(I)-mediated Fenton reaction. However, the antioxidant mechanistic details of MMI, particularly its structural analogy to the natural antioxidant ergothioneine (both of which feature an imidazole-2-thione moiety), remain largely unexplored due to the difficulty of in situ characterizing unstable reaction intermediates. In this study, we combined an in situ reactor-integrated paper-in-tip spray ionization source with mass spectrometry (PTSI-MS) to probe the protection mechanisms of MMI against the CuI-catalyzed Fenton reaction online. We present direct MS evidence of the antioxidant mechanisms of MMI (M) in its reduced form (MSH) and oxidized disulfide form (MSSM). The former can directly sequester H2O2 (i.e., a H2O2 scavenging mechanism). The latter tightly coordinates with CuI to form a CuI-MSSM complex that stabilizes CuI and prevents its oxidation by H2O2 and thus the formation of •OH (i.e., a metal ion coordination mechanism). The proposed reaction pathway of the CuI complexes from MMI disulfide (CuI-MSSM, m/z = 289 and 291) to MMI thiosulfonate (CuI-MSO2SM, m/z = 321 and 323) to MMI monosulfide (CuI-MSM, m/z 257 and 259) was verified by S–S oxidation, SO2–S bond cleavage, and extruded sulfur elimination, which confirmed the stable N,N′-bidentate binding of CuI to these intermediates, even when attacked by H2O2. These findings challenge the traditional radical scavenging mechanism and contribute to our understanding of the relationship between the structure and antioxidant activity of imidazole-2-thiones. Furthermore, the established in situ reactor-integrated PTSI-MS approach provides unique insights into reaction dynamics, offering the advantages of simplicity, speed, and low reaction volume (down to 20 μL).
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Ruiyang Qin,Fanyue Meng,Lijian Leng,Zhengyong Xu,Hailong Li,Shaowei Zhang
Thallium (Tl) and its compounds pose significant environmental risks due to their high toxicity and bioaccumulative potential, with industrial discharge standards mandating Tl concentrations below 5 μg/L. This study addresses the underexplored challenge of removing trace Tl(I) (≤1 mg/L) by investigating the efficacy of zinc sulfide (ZnS) as an adsorbent. Batch experiments, complemented by kinetic analyses (pseudo-second-order model), isotherm studies (Langmuir model, Qmax = 34.7 mg/g), and multi-technique characterization, were conducted to evaluate adsorption performance. Results show that ZnS achieves >99.9 % removal of trace Tl(I) at pH 10, reducing effluent concentrations to <0.4 μg/L—well below regulatory limits. Mechanistic investigations reveal a synergistic pathway: electrostatic attraction to negatively charged hydroxylated ZnS surfaces (zeta potential = −15.4 mV at pH 10), surface complexation via ≡ Zn-O-Tl bonds, and gradual formation of crystalline Tl₂S precipitates over prolonged contact. A two-stage treatment strategy, integrating sodium sulfide precipitation with ZnS adsorption, effectively purifies real-world matrices, including Tl-contaminated rainwater (Tl(I) from 352 μg/L to 0.440 μg/L) and high-salt industrial effluent (Tl(I) from 55.0 mg/L to 0.330 μg/L). This work provides experimental evidence for ZnS as a viable adsorbent for trace Tl(I) remediation, supporting the development of cost-effective heavy metal removal technologies.
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ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.6
Wanqing Zhang,Xiaodong Su,Yaxin Lv,Xuelin Zhao,Zhou Zhang,Yuntong Du,Mengyu Fan,Heshan Zhao,Matthias Rögner,Felipe Conzuelo,Weining Zhao,Mei Li,Fangyuan Zhao
Developing efficient semiartificial photosynthetic systems is crucial for sustainable solar energy conversion. Photosystem I (PSI) is an ideal photoactive component for such systems due to its ability to perform light-induced charge separation with near-perfect quantum efficiency. We report a high-performance PSI-based biophotocathode by immobilizing PSI in a poly(vinyl)imidazole-Os(bispyridine)2Cl (POs) redox polymer hydrogel within a translucent, three-dimensional (3D) porous indium tin oxide (ITO) scaffold. This hierarchical architecture synergistically enhances the PSI/redox hydrogel loading, promotes light harvesting, and facilitates rapid mediated electron transfer (MET), leading to record-breaking steady-state photocurrent densities of 4068 μA cm–2. An outstanding incident photon-to-current conversion efficiency (IPCE) of up to 61% is observed at a light intensity of 0.14 mW cm–2. To create a self-sustaining system, this advanced photocathode was integrated with a bioanode featuring galactose oxidase that efficiently oxidizes glycerol, a low-cost byproduct of the biodiesel industry. The resulting two-compartment photoelectrochemical (PEC) cell operates as a biophotovoltaic cell, exhibiting an open-circuit voltage of 0.42 V and a maximum power density of 18.5 μW cm–2. This work not only presents a significant advancement in the performance of PSI-based biophotocathodes but also demonstrates a viable pathway for semiartificial photosynthetic systems that simultaneously convert solar energy and valorize a waste stream chemical.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2026
·
IF 5.6
Yaqi Pu,Tianxin Li,Zihe Zhao,Qian Xu,Yi Jin,Huan Dou,Zhao Liu
Background Abdominal aortic aneurysm (AAA) is a prevalent and potentially fatal cardiovascular disease, yet the precise therapeutic mechanism of the bioactive compound Senkyunolide I (SEI) against AAA is not fully understood. Methods Utilizing an elastase/BAPN-induced AAA mouse model, this study employed integrated network pharmacology, proteomics (LiP-MS), and biophysical validation (molecular docking, MST) to identify and confirm PARP-1 as a direct target of SEI. The therapeutic effect of SEI was evaluated in vivo by monitoring aortic dilation and histopathology, and its inhibitory effect on TNF-α-induced macrophage migration was assessed in vitro . The functional role of the SEI-PARP-1 axis was further verified using PARP-1 inhibitors and macrophage-targeted PARP-1 overexpression. Results In the elastase/BAPN-induced AAA model, which mimics human AAA progression and rupture risk, SEI treatment attenuated disease progression by reducing aortic dilation, collagen deposition, and elastin degradation. SEI directly bound to and downregulated PARP-1 expression in both AAA tissues and large peritoneal macrophages (LPMs). It dose-dependently suppressed TNF-α-induced LPMs migration in vitro and restored the AAA-induced reduction in peritoneal LPMs proportion in vivo , an effect also seen with pharmacological PARP-1 inhibition. In contrast, macrophage-specific PARP-1 overexpression abolished SEI's protection and promoted LPMs migration into aortic lesions. Conclusion This study demonstrates that SEI attenuates AAA progression by targeting PARP-1 and inhibiting the migration of LPMs from the peritoneal cavity to the aortic lesion. These findings elucidate a novel pharmacological mechanism of SEI and underscore PARP-1 as a promising therapeutic target for AAA.
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JOURNAL OF STRUCTURAL CHEMISTRY
·
2026
·Vol. 67
·期 1
·65-74
·
IF 1.5
Chen Y. -X.,Qiu B.,Xu Y. -J.,Yan W. -B.,Liu S. -J.,Qiu X. -Y.
New cobalt(II) and copper(I) complexes, [CoCl 2 (L 1 ) 2 ] ( 1 ) and [CuClL 2 (Ph 3 P) 2 ]·CH 3 CN ( 2 ), where L 1 = 1-(4-bromobenzylidene)thiosemicarbazide, L 2 = 1-(2,4-dichlorobenzylidene)thiosemicarbazide, PPh 3 =
= triphenylphosphine, were synthesized and characterized by elemental analysis and physico-chemical method, as well as single crystal X-ray diffraction. The complexes were also tested for their urease inhibitory properties. The cobalt complex has strong activity ( IC 50 = 5.7 μM). Enzymatic kinetics research suggested that the cobalt complex is a non-competitive urease inhibitor.
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AGING CELL
·
2026
·Vol. 25
·期 2
·e70378
·
IF 7.1
Meiling Wang,Yumin Chang,Aojie He,Jing Yang,Ang Li,Hongqin Wang,Kah-Leong Lim,Xing Guo,Chengwu Zhang,Li Lu
Age-related memory decline is a hallmark of brain aging and a primary risk factor for neurodegenerative disorders. Microglia play a crucial role in preserving memory function by maintaining brain homeostasis through phagocytosis, yet the specific mechanisms governing this protective function remain elusive. In the present study, we identified a population of Secreted Phosphoprotein 1 (Spp1)-positive microglia in both aged mouse and human brains. To investigate the role of microglial Spp1 in aging, we generated microglia-specific Spp1 knockout (Spp1-cKO) mice. We demonstrate that Spp1 deficiency selectively precipitates memory deficits in aged mice, without affecting memory function in young mice, indicating an age-dependent reliance on Spp1 signaling. Microglial phagocytic capacity positively correlates with Spp1 levels and is diminished by Spp1 deficiency. Mechanistically, Spp1 deficiency leads to the downregulation of the AKT/mitochondrial complex I pathway, thereby compromising microglial oxidative phosphorylation and function. Notably, microglia-specific overexpression of Spp1 partially ameliorates the age-related phenotypes induced by Spp1 deficiency. In conclusion, this study is the first to reveal the crucial role of microglial Spp1 in brain aging and to uncover its underlying mechanism, providing novel insights into age-related memory decline.
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FOOD HYDROCOLLOIDS
·
2026
·
IF 12.4
Jiefen Cui,Xiaoxiao Zhang,Dan Liu,Jirong Wang,Yongkai Yuan,Jinkai Zheng
The relationship between pectin side chains and fermentability is crucial for understanding precision nutrition. In this study, α-L-arabinofuranosidase and β-galactosidase were used to remove arabinose and galactose side chains of citrus pectin. Effects of RG-I side chains on structural characteristics and in vitro fermentation property of pectin were investigated. The removal of arabinose and galactose side chains led to a decrease in molecular weight and branching degree of pectin, which in turn resulted in a loose spatial conformation. Based on correlation analysis and carbohydrate-active enzymes annotation, we concluded that the presence of arabinose in citrus pectin promoted the production of propionate and had no promoting effect on the proliferation of pathogenic bacteria of Escherichia and Klebsiella . The removal of the galactose side chain promoted proliferation of potentially beneficial bacteria ( Bifidobacterium , Faecalibacterium , Veillonella , and Parabacteroides ). We further found that arabinose produced propionate mainly via the succinate pathway and acrylate pathway, while galactose generated butyrate by activating phosphate butyryltransferase and butyrate kinase. Our results provide new insights into structure-function relationship between arabinose and galactose side chains and fermentability of pectin, while also offering a scientific reference for targeted design of pectin prebiotics with precise functionalities.
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