Phytochemistry Letters
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2020
·
IF 1.459
Fang-Fang Chen,Jing Zhou,Yu-Wei Zhang,Ya-Ping Chen,Yin-Ru Wang,Yun-Fang Zhao,Wei Liu,Xue-Feng Huang
Two novel cholestane-type steroidal saponins , melongoside R-S ( 1 - 2 ) and three novel furostanol-type steroidal saponins , melongoside T-V ( 3 - 5 ), together with three known steroidal saponins ( 6 - 8 ) were isolated from the seeds of Solanum melongna L. The structures of these compounds were elucidated by extensive spectroscopic analysis such as HR-ESI-MS, ESI-MS and NMR.
研究领域:
植物化学
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天然产物研究
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药物化学
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DOI
高影响因子
Journal of Pharmaceutical Analysis
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2019
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IF 4.44
Ting Liu,Zhaoqin Li,Ruiyun Li,Yue Cui,Yunli Zhao,Zhiguo Yu
The present investigation reports the chemical composition of the Rhus typhina L. stem identified via mass spectrometry and NMR as gallic acid, 1- O -galloyl- β - D -glucose, tryptophan, scopolin, methyl gallate, fustin, quercetin, rutin, and 1,2,3,4,6-penta- O -galloyl- β - D -glucose. The antioxidant properties and the chemical composition contents of the R. typhina L. stem grown in different regions in China were determined. To determine the antioxidant activity, a total phenolic content analysis, 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity assay, ferric reducing antioxidant power assay, and β -carotene linoleic acid model system were conducted. The results showed that the Rhus typhina L. stem possessed high antioxidant capacities due to its high phenolic content. The contents of the nine isolated compounds were determined by UPLC-ESI-MS/MS. The calibration curves of the nine isolated compounds were linear within the concentration range and the average recoveries were high. The result showed that 1- O -galloyl- β - D -glucose, gallic acid, methyl gallate, and 1,2,3,4,6-penta- O -galloyl- β - D -glucose could be the compounds mainly responsible for the antioxidant capacity of the R. typhina L. stem. This reveals that the R. typhina L. stem is a good source of antioxidants.
研究领域:
药用植物学
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分析化学
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植物科学
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
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2025
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IF 4.8
Dandan Gao,Xuankang Yang,Danqi Lu,Chenchen Wang,Jiwen Wang,Huaxin Li,Xingchen Guo
Introduction Potentilla anserina L. , a traditional Chinese medicinal herb, is valued for its edible, medicinal, and ornamental properties. Methods In this study, flavonoids were extracted from Potentilla anserina L. using ultrasonic-assisted extraction. The extraction process was optimized through response surface methodology, followed by separation and purification using Sephadex G-100 gel chromatography. Finally, the antioxidant activity of the extracted flavonoids was evaluated. Results The results showed that the optimal extraction conditions were 60% ethanol concentration, an ultrasonic temperature of 50°C, ultrasonic power of 400 W, and ultrasonic time of 180 min. Under these conditions, the average extraction yield of flavonoids from Potentilla anserina L. was 3.74 ± 0.06 mg/g. The crude flavonoid extract was purified by Sephadex G-100 gel chromatography, yielding two fractions, LF-1 and LF-2, which accounted for 63.34% and 25.79% of the crude extract, respectively. Discussion The results of in vitro antioxidant activity experiments demonstrated that both fractions (LF-1 and LF-2) exhibited significant antioxidant activity, showing a dose-dependent capacity. These findings provide a theoretical basis for the further development and utilization of flavonoids from Potentilla anserina L..
研究领域:
植物化学
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天然产物研究
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抗氧化研究
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
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2025
·
IF 4.9
Zhenhang Sun,Zhi-Yuan Yao,Xiaomei Zhang,Jin-Song Gong,Guoqiang Xu,Xiaojuan Zhang,Hui Li,Jin-Song Shi,Zheng-Hong Xu
L-Cysteine, a crucial amino acid containing an active thiol group, finds extensive applications across various industries such as pharmaceuticals, food, feed, and cosmetics. Despite its significance, the efficient production of l-cysteine has long been a challenging task. This study analyzed the l-cysteine synthesis pathway in microorganisms and designed a novel synthetic route for l-cysteine utilizing glycine as a primary substrate. By employing a cell-free synthetic system catalyzed by multi-enzyme cascades, efficient synthesis of l-cysteine was achieved. The study also implemented a high-throughput screening approach for identifying rate-limiting enzymes using a l-cysteine biosensor, leading to a notable 20.12 % enhancement in the activity of the key rate-limiting enzyme, L‑serine acetyltransferase. Furthermore, through the optimization of catalytic reaction conditions, the l-cysteine titer increased by 2.06-fold, reaching a peak of 19.44 mg/L. This innovative approach utilizing a multi-enzyme cascade pathway unveils promising new avenues for l-cysteine production.
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DOI
高影响因子
ANALYTICAL CHEMISTRY
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2015
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IF 5.636
Chuantao Hou,Shuqin Fan,Qiaolin Lang,Aihua Liu
l-cysteine (l-Cys) detection is of great importance because of its crucial roles in physiological and clinical diagnoses. In this study, a glucose/O2 biofuel cell (BFC) was assembled by using flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH)-based bioanode and laccase-based biocathode. Interestingly, the open circuit potential (OCP) of the BFC could be inhibited by Cu2+ and subsequently activated by l-Cys, by which a BFC-based self-powered sensing platform for the detection of l-Cys was proposed. The FAD-GDH activity can be inhibited by Cu2+ and, in turn, subsequent reversible activation by l-Cys because of the binding preference of l-Cys toward Cu2+ by forming the Cu–S bond. The preferential interaction between l-Cys and Cu2+ facilitated Cu2+ to remove from the surface of the bioanode, and thus, the OCP of the system could be turned on. Under optimized conditions, the OCP of the BFC was systematically increased upon the addition of the l-Cys. The OCP increment (ΔOCP) was linear with the concentration of l-Cys within 20 nM to 3 μM. The proposed sensor exhibited lower detection limit of 10 nM l-Cys (S/N = 3), which is significantly lower than those values for other methods reported so far. Other amino acids and glutathione did not affect l-Cys detection. Therefore, this developed approach is sensitive, facile, cost-effective, and environmental-friendly, and could be very promising for the reliable clinically detecting of l-Cys. This work would trigger the interest of developing BFCs based self-powered sensors for practical applications.
研究领域:
生物电化学
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生物传感器
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分析化学
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能量转换
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环境监测
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Junge Wei,Rui Qu,Yanhe Wang,Lei Liu,Jie Yang,Huidi Xu,Xiaohong Hu,Xiaofeng Song
Establishing drug release from shape memory polymers (SMPs) for biomedical applications will broaden the horizon of SMP applications from commercial medical device to scientific drug delivery system . Therefore, a strategy combining degradable SMP with drug release is put forward. However, there are few reports about the relevance between them so far. In the work, incorporations of three grafting tannins (TA) as switching phase into poly ( l -lactide)(PLLA) construct different thermoresponsive SM composites. TA-PCL-COOH/PLLA exhibites good shape fixation (R f ) and recovery rate (R r ) at 55 °C, and its recovery time is 75 s. After loading lipophilic drug, SM capability of medicated TA-PCL-COOH/PLLA enhances, the R f and R r are 97.8% and 97.2%, in particular, its recovery time decreases to 32 s. The effect of SM on drug release is explored. After the first round of SM, the drug release accelerates obviously at body temperature; for example, the release amount of drug increases from 46.5% to 66.1% at initial 12 h due to change of microstructure and improvement of wettability . The drug release rate climbs only slightly as the SM round increases.
研究领域:
生物医学工程
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药物递送系统
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高分子科学
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智能材料
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生物材料
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DOI
高影响因子
Environmental Science-Processes & Impacts
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2022
·Vol. 24
·期 4
·586-597
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IF 5.334
Dongqing Gong,Xue Bai,Yuzhu Weng,Mengen Kang,Yue Huang,Fengjie Li,Yanling Chen
Nanoplastics and metal oxide nanoparticles are serious threats that inevitably enter the environment. Their similar particle properties likely lead to interaction and thus cause more unpredictable ecotoxicity to organisms. In this study, it was found that polystyrene nanoplastics (PS NPs) aggravate the toxic effect of iron oxide nanoparticles (Fe2O3 NPs) on Lactuca sativa L. by inducing severe oxidative stress and root deformation, and the expansion of damaged cells from the xylem to the epidermis was observed using confocal laser scanning. Exposure to PS NPs + Fe2O3 NPs correspondingly elevated iron accumulation in the roots and leaves by 1.39 and 1.17 times compared to the amount observed with Fe2O3 NPs individually. Examination of the physicochemical properties, iron ion release, and molecular interactions of the NPs indicated that PS NPs interact with Fe2O3 NPs to form heteroaggregates and facilitate leaching of iron ions, which resulted in aggravating the toxic effect. These were alleviated by the addition of humic acid (HA), which dispersed the heteroaggregates and reduced the release of iron ions. The findings in the present study provide new perspectives for the ecotoxicological risk of binary nano-pollution in the natural environment.
研究领域:
环境科学
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植物毒理学
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DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Chen Wu,Zhongyun Zhang,Lijie Bai,Shuhui Lei,Min Zou,Zilu Bao,Zhaoxiang Ren,Kaiqun Liu,Hui-Hong Gong,Wenjun Ma,Lvyi Chen
Ethnopharmacological relevance Gout, a painful joint disease with a prevalence ranging from 0.86% to 2.2% in China over the past decade. Traditional medicine has long utilized the medicinal and edible Piper longum L. (PL) fruit spikes for treating gout and other joint conditions like rheumatoid arthritis. However, the exact mechanisms behind its effectiveness remain unclear. Aim of the study This study aimed to investigate the potential of alcoholic extracts from PL fruit spikes as a safe and effective treatment for gout. We used a combined network pharmacology and experimental validation approach to evaluate the mechanisms behind the anti-gout properties of PL. Materials and methods UPLC-Q/TOF-MS analysis determined the major components of PL. Subsequently, network pharmacology analysis predicted potential molecular targets and related signaling pathways for the anti-gout activity of PL. Molecular docking simulations further explored the interactions between PL compounds and proteins and characterized the properties of potential bioactive secondary metabolites. Mouse models of air pouch inflammation and hyperuricemia were further established, and the anti-gout mechanism of PL was confirmed by examining the expression of proteins related to the MAPK and PI3K-AKT pathways in the tissue. Results Our analysis revealed 220 bioactive secondary metabolites within PL extracts. Network pharmacology and molecular docking results indicated that these metabolites primarily combat gout by modulating the PI3K-AKT and MAPK signaling pathways. In vivo experiments have also proven that PL at a dose of 100 mg/kg can optimally reduce acute inflammation of gout and kidney damage caused by high uric acid. The anti-gout mechanism involves the PI3K-AKT/MAPK signaling pathway and its downstream NF-κB pathway. Conclusion This study provides compelling evidence for PL's therapeutic potential in gout management by modulating key inflammatory pathways. The findings offer a strong foundation for future clinical exploration of PL as a gout treatment option.
研究领域:
药理学
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民族药理学
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分子生物学
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炎症研究
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传统医学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
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2024
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IF 4.1
Liu Fan,Huang Shao-Yong,Tang Jian,Chen Quan
In this study, we prepared unentangled and slightly entangled poly( L -lactic acid) telechelic ionomer samples ( M n =5 and 16 kg/mol) based on sodium sulfonate groups. The telechelic samples exhibit extremely slow crystallization kinetics below the melting temperature T m and above the glass transition temperature T g , which enables us to examine the linear viscoelasticity of the ionomer melt samples therein. The application of either the shear flow (at 85 °C) or elongational flow (between 70 and 90 °C) strongly accelerates the crystallization, leading to strong strain hardening and formation of highly oriented α crystals. Depending on the relative average rates of the strain-induced dissociation and strain-induced crystallization, the stress evolution can be classified into two cases, and the critical work for strain-induced crystallization is higher in case where the strain-induced dissociation occurs earlier than the strain-induced crystallization.
研究领域:
高分子化学
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材料科学
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DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·Vol. 79
·期 10
·3570-3580
·
IF 4.1
Weiting Jiao,Ping Zhang,Chuanjian Cui,Min Yan,Qing X. Li,Yongfeng Tang,Nan Zhang,Xinyi Wang,Ruyan Hou,Rimao Hua
Background Thiamethoxam (TMX) is insecticidal, but also can trigger physiological and metabolic reactions of plant cycles. The objective of this work was to evaluate the physiological and metabolic effect of TMX on tea plants and its potential benefits. Results In this study, dose of TMX (0.09, 0.135 and 0.18 kg a.i./ha) were tested. Except for peroxidase (POD) and glutathione S-transferase (GST), chlorophyll, carotenoid, catalase (CAT) and malondialdehyde (MDA) were significantly affected compared with the controls. The CAT activity was increased by 3.38, 1.71, 2.91 times, respectively, under three doses of TMX treatment. The metabolic response between TMX treatment and control groups on the third day was compared using a widely targeted metabolomics. A total of 97 different metabolites were identified, including benzenoids, flavonoids, lipids and lipid-like molecules, organic acids and derivatives, organic nitrogen compounds, organic oxygen compounds, organoheterocyclic compounds, phenylpropanoids and polyketides, and others. Those metabolites were mapped on the perturbed metabolic pathways. The results demonstrated that the most perturbation occurred in flavone and flavonol biosynthesis. The beneficial secondary metabolites luteolin and kaempferol were upregulated 1.46 and 1.31 times respectively, which protect plants from biotic and abiotic stresses. Molecular docking models suggest interactions between TMX and flavonoid 3-O-glucosyltransferase. Conclusion Thiamethoxam spray positively promoted the physiological and metabolic response of tea plants. And this work also provided the useful information of TMX metabolism in tea plants as well as rational application of insecticides. © 2023 Society of Chemical Industry.
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PubMed
高影响因子
Nanomaterials
·
2023
·Vol. 13
·期 1
·202
·
IF 5.3
Jingyu Li,Yang Zhang,Haichao Zhao,Guoxin Sui
The excessively leached metal ions from traditional metallic antimicrobial nanoparticles are harmful to biological and human tissues. Metal-organic frameworks (MOFs) coordinating bioactive metal ions to organic bridging ligands can potentially address this issue, avoiding the excessive leaching of metal ions and simultaneously exhibiting high effective antibacterial activities. Here, we report the preparation of a 2-dimensional leaves-like zeolitic imidazolate framework (ZIF-L) for potential antibacterial and anti-algae applications. The ZIF-L nanosheet exhibits complete inactivation ofEscherichia coli(phosphate buffer saline: 4 h) andBacillus subtilis(seawater: 0.5 h). The ZIF-L/epoxy composite has excellent antibacterial effect, poisoning effect and anti-adhesion effect on a variety of marine algae. It is worth noting that the removal rate (Escherichia coli) for ZIF/epoxy composite can be reached to 90.20% by only adding ZIF-L (0.25 wt%). This work will inspire researchers to develop more metal-organic frameworks materials for applications in the antibacterial and anti-algae fields.Keywords:ZIF-L;two dimensional;antibacterial;anti-algae
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yadong Wang,Guosheng Niu,Xiaofei Cao,Zhichao Yang,Yuan Dong,Guitao Feng,Yingliang Liu,Jizheng Wang,Shaokui Cao
Organic-inorganic hybrid perovskites have already been employed as promising light-sensitive materials for high-performance photodetectors (PDs) due to their intriguing optoelectronic features. Nonetheless, such perovskite-based PDs often suffer from low sensitivity, high dark current and inevitable degradation. In this work, a novel multifunctional molecule l -tryptophan ethyl ester hydrochloride ( l -TEEH) was used to treat the surface defects of triple-cation mixed halide perovskite film. It is found that the modulator with multiple functional groups can effectively passivate the uncoordinated ions (Pb 2+ and I - ) and halide (I and Cl) vacancies of the perovskite film. Owing to the reduced defects, the dark current of the pristine perovskite PD significantly decreases from ∼ 10 -9 A to ∼ 10 -11 A after the l -TEEH treatment. Moreover, the perovskite PD with the l -TEEH treatment exhibits a large photoresponsivity (1.27 A/W) and an ultrahigh on/off ratio (∼9 × 10 3 ). More impressively, the optimized device shows excellent stability in air with a relative humidity of 40–50 %. Furthermore, the reliable imaging capability of perovskite device with the l -TEEH treatment is also successfully demonstrated.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）