LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Zhe Dong,Yu Zhao,Jinliang Chen,Ming Chang,Xiaosan Wang,Qingzhe Jin,Xingguo Wang
Borneol esters have attracted increasing interest due to their potential application in the food industry. In this study, the extraction of d -borneol from Cinnamomum camphora chvar. borneol seed was carried out via steam distillation to gain highly purified d -borneol (99.66%). The lipase-catalyzed lipophilization of extracted d -borneol with oleic acid was performed in a solvent-free system. The influences of lipase type, substrate molar ratio, lipase loading, reaction temperature and reaction time on d -bornyl oleate yield were evaluated. Among four kinds of commercial lipases screened for their esterification efficiency, CRL (lipase from Candida rugose ) displayed the highest catalytic activity in the synthesis of d -bornyl oleate. The yield of more than 95% was obtained under the optimal conditions: substrate molar ratio 1:2 ( d -borneol/oleic acid), lipase loading 8%, reaction temperature 45 °C, reaction time 48 h. The structural identification of d -borneol and product d -bornyl oleate was performed by gas chromatography mass spectrometry (GC-MS) and proton nuclear magnetic resonance spectroscopy ( 1 H NMR). The results showed that the product was confirmed to be d -bornyl oleate and the purity of which was 97.51%. This study will pave the way for d -borneol and its esters application in food industry.
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Scientific Reports
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2021
·Vol. 11
·期 1
·1-9
·
IF 4.38
Dong Wenjing,Song Erqun,Song Yang
The acute liver injury (ALI) and hepatic fibrosis caused by the co-treatment of lipopolysaccharide (LPS)/ d -galactosamine (D-GalN) have been extensively studied. However, whether LPS/D-GalN are genotoxic has been left unknown. In this study, male mice were divided into eight groups with eight animals in each group. For acute challenge of LPS/D-GalN, the mice in each group received a combination of LPS/D-GalN via intraperitoneal injection at the dose of 25 μg/kg/250 mg/kg, 25 μg/kg/500 mg/kg, or 50 μg/kg/500 mg/kg body weight. An additional group for chronic administration of test compounds was conducted by i.p. injection of LPS/D-GalN (10 μg/kg/100 mg/kg) every other day for 8 weeks. Saline solution (0.9%) and cyclophosphamide (CTX) (50 mg/kg body weight) given by i.p. injection was used as the negative and positive control, respectively. The results of single cell gel electrophoresis (SCGE) assay indicated that acute exposure of the mice to LPS/D-GalN caused severe DNA damage in hepatic cells, but not in the brain, sperm or bone marrow cells, which evidenced the genotoxicity of LPS/D-GalN administrated in combination. Interestingly, the chronic administration of LPS/D-GalN triggered significant genotoxic effects not only in hepatic but also in brain cells, with negative results in sperm and bone marrow cells. Histopathological examination in the liver and brain tissues revealed changes consistent with the SCGE results. The present study indicates genotoxic potential of LPS/D-GalN co-administered in mice, which may serve as an in vivo experimental model for relevant genotoxic study.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Jiping Li,Jing Zhang,Langqiu Chen,Guochao Zhang,Jingyi Liao
Hydrophilic alkyl polyglycosides (APGs) and alkyl glycosides (AGs) with anomeric pure are a class of important substitutes for petroleum-based surfactants. Improving their water solubility should make such hydrophilic glycosurfactants have more excellent potential application value. To solve the inherent problem of poor water solubility of traditional alkyl β-d-glucopyranoside (5), a series of alkyltetra(oxyethyl) β-d-glucopyranosides (4a–4g, n = 7–18) were successfully synthesized by introducing tetra(oxyethylene) fragments to carry out the structural modification. The relationship between the related structure and the physicochemical properties was further investigated, including their hydrophilic–lipophilic balance (HLB), water-solubility, foaming performance, emulsification, hygroscopicity, surface activity, and thermotropic/lyotropic liquid crystal phase behavior. The results showed that the water solubility gradually decreased as the alkyl chain length increased due to the gradual decrease of their HLB number. Octadecyltetra(oxyethyl) β-d-glucopyranoside (4g, n = 18) was found to be insoluble in water at 25 °C. Taken together, long-chain alkyl glycosides had good foaming properties and excellent emulsifying properties. Among them, dodecyltetra(oxyethyl) β-d-glucopyranoside (4d, n = 12) had the best foaming performance. In the rapeseed oil/water system, cetyltetra(oxyethyl) β-d-glucopyranoside (4f, n = 16) had the best emulsifying ability. With the increase of the alkyl chain length, the critical micelle concentration (Ccmc), γcmc, Γmax, and hygroscopicity of this series of glycosides showed a downward trend. Differential scanning calorimetry (DSC) and polarizing optical microscopy (POM) showed that the thermal stability increased with the increase of the alkyl chain length, and alkyltetra(oxyethyl) β-d-glucopyranosides (4d–4g, n = 12–18) had the corresponding melting points and clearing points. Alkyltetra(oxyethyl) β-d-glucopyranosides (4b–4g, n = 8–18) formed a smectic phase with a typical fan-shaped and focal conic texture during the cooling process. In the water contact experiments, it was found that glycosides (4b–4g, n = 8–18) at high concentrations transformed into various lyotropic liquid crystal including hexagonal phase, bicontinuous cubic phase, and lamellar phase phases. Therefore, such green nonionic glycosurfactants alkyltetra(oxyethyl) β-d-glucopyranosides should have potential practical application prospects.
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高影响因子
LANGMUIR
·
2019
·
IF 3.683
Shanshan Wu,Zheng-Zhi Yin,Datong Wu,Yongxin Tao,Yong Kong
π-Conjugated porphyrins have aroused particular attention for nanofabrication and biomimics; however, little attention has been paid to porphyrins-based chiral analysis owing to the achiral feature of porphyrins. Here, we demonstrated a chiral self-assembly of achiral porphyrin induced by l- and d-lysine (l- and d-Lys), and the resultant porphyrin self-assembly exhibited alterable morphologies depending on the inducer used (l- or d-Lys). The supramolecular chirality of the self-assembly was characterized by circular dichroism (CD) spectra, confirming successful transfer of molecular chirality from l- and d-Lys to the self-assembly. The enantioselective property of the chiral self-assembly was also investigated by using tryptophan (Trp) isomers as the model, and the results indicated that the developed chiral self-assembly showed significantly higher affinity toward l-Trp than d-Trp. Also in this work, the l-/d-Lys-induced chiral self-assembly of porphyrin and the supramolecular interaction between the self-assembly and l-/d-Trp were also studied by density functional theory (DFT).
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2019
·
IF 14.229
Yu-Xuan Xiao,Jie Ying,Ge Tian,Yong Tao,Hao Wei,Si-Yu Fan,Ze-Hao Sun,Wan-Juan Zou,Jie Hu,Gang-Gang Chang,Weihua Li,Xiao-Yu Yang,Christoph Janiak
The strong Pt-carbon interaction is a very promising way to high-performance design of electrocatalysts. However, the heavy d-π overlap, a high strength of Pt-C bonding, in sp 2 bonded graphitic carbons with low level of defects is rarely reported. Herein, PtPd nanoparticles and graphitic carbon nanofibers (PtPd/CNF) have been integrated via heavy d-π overlap by an interfacial wettability approach and their formation mechanism has also been fully elucidated. The features of strong d-π overlap and high dispersion of metals offer PtPd/CNF with enhanced ORR activity (5.8 folds of specific activity than commercial Pt/C), and excellent structural stability and electrocatalytic durability. A new “heavy d-π overlap” concept, characteristics, and mechanism are proposed at an atomic-/nanoscale to clarify the generation of the predictive interaction as well as the interface charge transfer. It is believed that the study on heavy d-π overlap sheds new light on the fundamental aspects of the nature of Pt-C interaction.
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AMINO ACIDS
·
2018
·Vol. 50
·期 9
·1269-1278
·
IF 2.906
Wu Pinping,Hu Fan,Wang Ruya,Gao Lingxuan,Huang Tao,Xin Yufu,He Hua
A new colorimetric analysis approach for chiral recognition of d - and l -forms of phenylalanine (phe) was developed based on triangular silver nanoplates (TAg-NPs). The TAg-NPs could be used as chiral colorimetric probes for d - and l -forms of phe. Upon addition of d -phe to TAg-NPs solution, a color change from blue to purple to pink could be observed, while no obvious color change was found on addition of l -phe. l -phe could prevent the TAg-NPs from being etched to small size particles while the protective effect of d -phe was weak. Moreover, the enantiomeric excess of d -phe could be determined using the proposed chiral assay in the percentage of l -phe from 0 to 100% with a correlation coefficient of 0.9855. The phenomenon could be monitored by bare eyes and quantified analysis by UV–Vis spectrophotometry. The developed approach had several advantages, such as simplicity, visualization, short analysis time and low cost. This study presented a fast visualization analysis method of chiral d / l -phenylalanine and may lay the foundation for the development of visualization chiral recognition of other target analytes.
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高影响因子
BIOMEDICINE & PHARMACOTHERAPY
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2018
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IF 3.457
Yuhui Wang,Jinyang Shen,Xiaolin Yang,Ye Jin,Zhonglin Yang,Rufeng Wang,Fuming Zhang,Robert J. Linhardt
Background Glucocorticoid hormones are implicated in the pathogenesis of Alzheimer’s disease (AD) and other diseases including diabetes, hyperlipidemia , and osteoporosis . Akebia saponin D (ASD) possesses numerous pharmacological activities , including as an anti-AD, anti-hyperlipidemia, anti-diabetes, and anti-osteoporosis agent. The anti-AD effect of ASD is possibly through its regulation of glucocorticoid levels. Purpose The present study was undertaken to investigate the neuroprotective effects of ASD on Aβ 25-35 -induced cognitive deficits and to elucidate its underlying mechanism of action. Methods The AD rat model was established by an intracerebroventricular injection of Aβ 25-35 into the lateral ventricles. Spatial learning and anxiety state were assessed by Morris water maze task and elevated plus-maze assay, respectively. The degree of hypertrophy of adrenal gland was analyzed using the viscera coefficient. Corticosterone and ACTH concentrations in the plasm were measured using biochemical assay kits. The activity of 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1) in liver and groin fat pad was assessed by measuring cortisol production. Results Compared with the control group, AD rats displayed significant spatial learning and reference memory impairments, serious anxiety disorders, obvious hypertrophy of adrenal gland, elevated corticosterone and ACTH levels in the plasma, and increased 11β-HSD1 activity in liver and groin fat pad. ASD could significantly ameliorate the memory deficits and anxiety symptoms, markedly reduce the viscera coefficient of adrenal gland, observably decrease corticosterone and ACTH concentrations, and showed no effect on the activity of 11β-HSD1. Conclusions These results indicate that ASD might exert a significant neuroprotective effect on cognitive impairment, driven in part by reducing systemic corticosterone level by down-regulation of the hypothalamic-pituitary-adrenal (HPA) axis.
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Natural Products and Bioprospecting
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2018
·Vol. 8
·期 6
·413-418
·
IF 0
Wang Xia,Peng Xing-Rong,Lu Jing,Hu Gui-Lin,Qiu Ming-Hua
Abstract In present study, four new dammarane-type triterpenoids, namely caffruones A–D ( 1 – 4 ), were isolated from the cherries of Coffea arabica . Their structures were elucidated by extensive spectroscopic analysis including 1D, 2D NMR (HSQC, HMBC, 1 H– 1 H COSY, and ROESY), HRMS and IR spectra. This is the first time that tetracyclic triterpenes have been reported in genus Coffea . Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2016
·Vol. 408
·期 29
·8317-8323
·
IF 3.125
Yan Zequn,Xue Min,He Qian,Lu Wei,Meng Zihui,Yan Dan,Qiu Lili,Zhou Lijun,Yu Yingjie
A novel polymerized crystalline colloidal array (PCCA) sensing material for the detection of urine glucose was developed by embedding a two-dimensional (2-D) polystyrene crystalline colloidal array (CCA) in 3-acrylamidophenylboronic acid (3-APBA)-functionalized hydrogel. After adjusting the cross-linker concentration, this material showed significant sensitivity for glucose under lab conditions, the particle spacing of the PCCA changed from 917 to 824 nm (93 nm) within 3 min as the glucose concentration increased from 0 to 10 mM, and the structural color of the PCCA changed from red through orange, to green, and finally, to cyan. In further experiments, this material was used to semi-quantitatively detect glucose in 20 human urine (HU) samples. Compared with the traditional dry-chemistry method, which was applied widely in clinical diagnosis, the PCCA method was more accurate and cost-effective. Moreover, this method can efficiently avoid the errors induced by most of the urine-interfering elements like vitamin C and ketone body. With a homemade portable optical detector, this low-cost intelligent sensing material can provide a more convenient and efficient strategy for the urine glucose detection in clinical diagnosis and point-of-care monitoring.
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Analytical Methods
·
2024
·
IF 2.7
Wu Bing,Sun Xiaoyu,Shao Zeman,Sen Liu,Zhongmei Yang,Qiuyang Zhang,Yanchun Wei
Mercury ions (Hg2+)are highly toxic heavy metal ions, posing serious health risks to humans when present above safe concentrations1–3. Therefore, the development of a rapid and effective Hg2+ detection method is of significant importance. In this study, based on surface plasmon resonance (SPR) technology integrated with COMSOL simulation analysis, a highly sensitive and selective Hg2+ sensing system is constructed. Initially, the gold nanoparticles and the surface of the fiber optic gold film are modified by sodium sulfide (Na2S). In the presence of Hg2+, the modified sulfur ions specifically bind to Hg2+, forming a composite structure Au/S-Hg2+-S/AuNPS. Due to the strong electromagnetic coupling between gold nanoparticles and the gold film, a significant SPR wavelength shift occurs. Experimental results demonstrate the high sensitivity of the sensor to Hg2+. The fiber optic SPR transducer exhibits enhanced selectivity towards Hg2+ ions and high recovery rates in real environmental samples. This sensing system provides an alternative method for rapid and accurate determination of mercury content.
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PROCESS BIOCHEMISTRY
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2024
·
IF 3.7
Puhong Yi,Yue Xu,Hanlin Liu,Yuhua Hao,Mengdan Liu,Zhiqiang Liu,Yaping Xue,Liqun Jin,Yuguo Zheng
2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid (PPO) is a valuable prochiral keto acid for the asymmetric synthesis of the important herbicide L-phosphinothricin (L-PPT). Developing an efficient biocatalytic pathway to meet the increasing demand for PPO is highly attractive. In this study, a robust D-amino acid aminotransferase from Cytobacillus firmus ( Cf DAAT) was screened, which exhibited excellent tolerability to D,L-PPT and showed remarkable specific activity and affinity toward pyruvate (61.9 U/mg, Km = 7.4 mM). A designed co-substrate regeneration system was then introduced to shift the reaction equilibrium forward by coupling with D-amino acid oxidase (DAAO) and catalase (CAT). An efficient whole-cell biocatalyst was obtained by fine-tuning RBS intensity to coordinate the co-expression of the three enzymes. Moreover, the optimum conditions to promote overall conversion efficiency were identified by response surface methodology. Consequently, with only a catalytic amount of pyruvate (3.33 mol%) added in a 1 L reaction system, 200 mM D-PPT (400 mM D,L-PPT) was completely converted in 10 h, resulting in a PPO productivity of 3.33 g⋅L −1 ⋅h −1 , and a notable conversion rate of >99% at 300 mM D-PPT in 16 h. It demonstrated a considerable catalytic level and provided a competitive route for industrial PPO production from D,L-PPT.
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JOURNAL OF BIOLOGICAL CHEMISTRY
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2024
·
IF 4
Chunxiu Liu,Kailiang Ma,Li Jiang,Xumei Liu,Yang Tong,Sen Yang,Xinghua Jin,Yifeng Wei,Yan Zhang
The sulfite-reducing bacterium Bilophila wadsworthia , a common human intestinal pathobiont, is unique in its ability to metabolize a wide variety of sulfonates to generate sulfite as a terminal electron acceptor (TEA). The resulting formation of H 2 S is implicated in inflammation and colon cancer. l -cysteate, an oxidation product of l -cysteine, is among the sulfonates metabolized by B. wadsworthia , although the enzymes involved remain unknown. Here we report a pathway for l -cysteate dissimilation in B. wadsworthia RZATAU, involving isomerization of l -cysteate to d -cysteate by a cysteate racemase ( Bw CuyB), followed by cleavage into pyruvate, ammonia and sulfite by a d -cysteate sulfo-lyase ( Bw CuyA). The strong selectivity of Bw CuyA for d -cysteate over l -cysteate was rationalized by protein structural modeling. A homolog of Bw CuyA in the marine bacterium Silicibacter pomeroyi ( Sp CuyA) was previously reported to be a l -cysteate sulfo-lyase, but our experiments confirm that Sp CuyA too displays a strong selectivity for d -cysteate. Growth of B. wadsworthia with cysteate as the electron acceptor is accompanied by production of H 2 S and induction of Bw CuyA. Close homologs of Bw CuyA and Bw CuyB are present in diverse bacteria, including many sulfate- and sulfite-reducing bacteria, suggesting their involvement in cysteate degradation in different biological environments.
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