CHEMICAL GEOLOGY
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2025
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IF 3.6
Xianghao Zha,Guangxia Liu,Ning Chen,Yu Zeng,Juan Wang,Ziyan Yang,Huan Tang,Guodong Fang
The iron and sulfur species play key roles in the transformation and immobilization of heavy metals (e.g., As(III)) in soil environments, while the underlying mechanism of these processes was not fully explored. In this study, the effects of sulfite (an important intermediate of sulfur species) on As(III) transformation induced by troilite (FeS) were investigated. Results showed that the presence of sulfite not only greatly enhanced As(III) oxidation by FeS, with k obs increased by approximated 30 times, but also significantly increased the As(III) immobilization on FeS surface. Further analysis reveal that sulfate radicals (SO 4 •− ) and hydroxyl radicals ( • OH), derived from the redox reactions of surface Fe(II)/Fe 2+ with sulfite, were the main contributors to As(III) oxidation. In addition, As(III) oxidation was significantly enhanced in the sulfite/FeS system over a wide pH range, and oxygen was a prerequisite for the generation of free radicals. Besides, the dissolved As can be efficiently immobilized on FeS surface through co-precipitation with amorphous Fe(OH) 3 phases, which was negligibly affected by the presence of anions and organic matters. This study provides new insights into the mechanism by which sulfur species drive the transformation and immobilization of As(III) during oxygenation of iron-sulfide minerals.
研究领域:
环境科学
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土壤化学
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产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
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2024
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IF 4.8
Yang Zhou,Zhenzhu xia,Frank Peprah Addai,Jinping Chen,Chengxiang Feng,Zongjian Zhen,Juan Han,Feng Lin,Zhirong Wang,Yun Wang
Most pristine metal organic frameworks (MOFs) are inherently microporous. To introduce desired porosity for easy enzyme infiltration and immobilization, polystyrene spheres was used as sacrificial template to design hierarchical ordered micro-mesoporous truncated single-crystalline ZIF-8 (SOM-ZIF-8). The surface area and pore diameter of the SOM-ZIF-8 were 821 m 2 /g and 7.09 nm which changed to 669 m 2 /g and 4.98 nm respectively, after lipase immobilization, suggesting the pores and surface of the SOM-ZIF-8 served as binding sites for the enzyme, and could reach a loading capacity of 134 mg/g after 24 h. The optimal conditions for achieving maximum lipase activity for free LipaseELP, ZIF-8@LipaseELP and SOM-ZIF-8@LipaseELP were pH 7.8 at 45 °C, and maintained 33.67 %, 45.6 % and 57.78 % residual activity after incubation at 80 °C for 2 h. The specific activity towards tributyrin and p-nitrophenyl acetate were observed to be SOM-ZIF-8@LipaseELP (0.25 and 45.85 U/mg) compared to ZIF-8@LipaseELP (0.211 and 43.62 U/mg) and LipaseELP (0.179 and 41.09 U/mg) respectively. The SOM-ZIF-8@LipaseELP could further be recovered and reused for 9 rounds while maintaining 73.8 % of its original activity. This study demonstrates that introducing mesoporous structures into ZIF-8 could improve its binding enzyme property for enhanced hydrolytic function.
研究领域:
材料科学
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生物技术
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Pirah Ayaz,Xi Liu,Zhi Chen,Yuyun Yu,Javaria Khayaban E. Erum,Tingting Sun,Hongyan Diao,Xiangdong Liu
The immobilization of antibacterial peptides (APs) onto silk material substrates remains challenging due to the scarcity of green, low-cost, and efficient methods for covalent bonding between these two proteins. The present work proposed a novel strategy for covalently grafting APs onto silk fiber surfaces for antibacterial purposes. Firstly, silk fiber surfaces were grafted with glutamine (Gln) through a cure-induced esterification reaction, the AP protamine (PM) was then covalently grafted via transglutaminase (TGase)-catalyzed cross-linking onto the fiber surfaces. FTIR, SEM, XPS investigations verified the successful attachment of PM and the covalent connection between the PM and the fiber surfaces. The modified silk fabric showed bacteria reduction rates higher than 99.99 % against S. aureus and E. coli , and maintained its antibacterial properties after 20 washing cycles, highlighting a desirable antibacterial activity and considerable durability. Moreover, the modifications did not sacrifice the appearances and wearing comfortabilities of silk fabric, but even led to significant enhancement in the moisture transfer properties. This strategy, based on naturally occurring amino acid, AP and enzyme, could not only be an attractive alternative for real-world application in the silk industry but also could be extended to the modification of other protein-based materials.
研究领域:
材料科学
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生物化学
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纺织工程
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2025
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IF 3.8
Hui Fang,Yuanyuan Deng,Zhihao Zhao,Jiarui Zeng,Mingwei Zhang,Pengfei Zhou
Improving catalytic performance represents a highly desirable but challenging objective for the immobilization of biocatalysts. A novel biocatalyst of ANL@Fe 3 O 4 -SiO 2 -APTES was fabricated by immobilizing Aspergillus niger lipase (ANL) onto amino-alkyl group-modified magnetic nanoparticles. This biocatalyst exhibited an enzyme loading of 127.68 mg/g and the specific activity of 1132.26 U/g. The superior activity and recyclability of ANL@Fe 3 O 4 -SiO 2 -APTES was validated. The outstanding performance for the enzymatic interesterification (EIE) of palm stearin (PS) and rice bran oils (RBO) was observed. The significantly decreased the solid fat content (SFC) and enhanced crystallization rate of oils was achieved, as well as the β' type of oils after EIE was obtained. Moreover, molecular dynamics simulations were conducted to elucidate the underlying catalytic mechanism of EIE. This study proposes a highly efficient and sustainable approach for the chemical modification of lipids, thereby promoting its broad application in the food industry.
研究领域:
生物化学
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食品科学
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催化技术
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产品关联
DOI
高影响因子
ANALYST
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2025
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IF 3.3
Nan Lin,Xiao Han,Shuo Tian,Dianping Tang,Danfeng Zhang
Interleukin-6 (IL-6) protects neurons by inhibiting the expression of factors related to neuronal injury in nervous headache patients. The development of rapid and sensitive IL-6 detection methods are very advantageous for easing the pain of the patients. In this work, an immobilization-free immunodetection method is explored for the voltammetric screening of IL-6 in serum samples from nervous headache patients. Initially, methylene blue-encapsulated liposome (MBLS) labeled with anti-IL-6 detection antibodies are confined into anti-IL-6 capture antibody-coated microplate through a sandwich-type immunoreaction, and subsequently subject to lysis treatment. After that, the lytic solution is transferred into a detection cell including Nafion-modified working electrode. Methylene blue molecules with positive charge are captured onto the negatively charged Nafion membrane, thus generating a voltammetric signal. The voltammetric peak currents are relative to the IL-6 amount in the solution. At the optimized experimental situations, MBLS-based split-type electrochemical sensing protocols have acceptable voltammetric currents for IL-6 from 0.01 to 100 pg mL-1, and allow screening of IL-6 as low as a concentration of 9.1 fg mL-1. The batch-to-batch coefficients of variation were ≤11.95%. A good anti-interference capability was acquired against other biomolecules. Seven human serum specimens and two diluted serum samples including IL-6 obtained from nervous headache patients were determined by MBLS-based electrochemical immunoassay, and achieved well-matched results in comparison with those of IL-6 ELISA protocol.
研究领域:
生物医学工程
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免疫学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FUEL
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2025
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IF 7.5
Chengda Yu,Haiwei Jiang,Jinming Shi,Huajun Huang,Liangliang Fan,Zhenlin Wen,Xianbin Ai,Zhigang Que,Qin Li,Chunbao Charles Xu,Weiran Yang
Hydrothermal liquefaction (HTL) effectively convert hyperaccumulators into valuable hydrochars and bio-oils. However, the treatment of high concentrations of heavy metals in the aqueous phase products of HTL is crucial for ensuring the sustainability of the phytoremediation industry. This study demonstrated the effects of steel slag (SS) on the immobilization, distribution, and environmental risk of heavy metals during the HTL of Pteris vittata L. (PVL). The introduction of SS in HTL resulted in maximum bio-oil yield and HHV of 25.35% and 30.27 MJ/kg, respectively, while promoting the deoxygenation of bio-oil. The steel slag-assisted HTL effectively immobilized arsenic from aqueous phase to solid phase in PVL, and the arsenic removal content reached 1584.62 mg/kg, with a maximum arsenic stabilization rate of 93.29%. Model compound experiments suggest that the mechanism of arsenic stabilization in SS involves the formation of co-precipitates by the reaction of metal oxides with arsenate and arsenite. Furthermore, the SS-assisted HTL effectively reduced the risk assessment value and bioavailability of heavy metals in PVL. Overall, this study presents a promising approach for immobilizing arsenic, increasing bio-oil production, and promoting environmentally safe treatment of As-enriched biomass and steel slag.
研究领域:
环境科学
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能源与资源工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Zichen Wang,Tongyue Zhu,Hu Sun,Geling Kuang,Yuxiao Feng,Jiandong Cui
The encapsulation of enzymes in ZIF-8 (enzyme@ZIF-8 composites) via co-precipitation has attracted considerable attention. However, enzyme@ZIF-8 composites often exhibit low activity due to the three-dimensional (3D) structural characteristics of ZIF-8 and the protonation of the precursor, 2-methylimidazole (2-MeIm). In this study, a novel 2D catalase@ZIFs(SO4) composite was synthesized as an alternative to the conventional 3D catalase@ZIF-8 composites. The 2D catalase@ZIFs(SO4) composites demonstrated a nine-fold increase in activity and three-fold higher enzyme loading compared to their 3D counterparts. The enhanced catalytic performance is attributed to the 2D structure of the composites and the partial replacement of 2-MeIm with sulfate during enzyme immobilization, which reduces the protonation of 2-MeIm, enhances the hydrophilic microenvironment, and facilitates substrate transfer. Furthermore, compared to free enzymes, the 2D catalase@ZIFs(SO4) composites exhibited an expanded pH tolerance range, superior thermal stability, enhanced resistance to denaturants, and improved storage stability. To validate the concept, glucose oxidase, glutamate oxidase, and phenylalanine ammonia lyase were immobilized using the same approach. All immobilized enzymes demonstrated increased activity relative to the traditional CAT@ZIF-8 composites. This study offers a versatile platform for enzyme encapsulation within ZIF-8 through co-precipitation.
研究领域:
生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
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2025
·
IF 2.6
Shiyu Li,Kai Xu,Shanpeng Ma,Xueying Qin,Li Jiang,Xiaohong Hou,Zibo Dong,Xun Gao,Lingling Huo
Currently, coronary heart disease (CHD) is prevalent worldwide, with its harmful impact second only to that of malignant tumors. A growing number of studies have confirmed that Choerospondias axillaris can significantly improve the therapeutic effect of CHD treatment and reduce the incidence of adverse reactions. However, its bioactive components and pharmacological mechanisms remain unclear. Based on a targeted fishing strategy, this study synthesized magnetic nanomaterials immobilized with 5-lipoxygenase (5-LOX) for screening and identifying 5-LOX inhibitors from the crude extract of Choerospondias axillaris . A total of 21 active components were identified and analyzed using ultra-high performance liquid chromatography-Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS/MS). By comparing the screened active components with reported 5-LOX inhibitors, 9 novel 5-LOX inhibitors were discovered. Further molecular docking was performed on these 9 inhibitors, and the pharmacological activity of the selected ligands was verified using a 5-LOX inhibitor screening kit (fluorescence method).The compounds are ranked by activity from strongest to weakest: Lobetyolin (23.92 μM), Anhydroicaritin (29.55 μM), Narirutin(33.66 μM), Fisetin (47.28 μM) and Naringin(59.02 μM). This novel enzyme-immobilized targeted fishing technology is expected to efficiently and accurately screen bioactive components targeting different receptors from complex substrates.
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DOI
PubMed
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2025
·
IF 6.2
Sihao Wang,Xiaoling Zhang,Yanghai Zheng,Yun Xue,Jingping Wang,Yongde Yan
Zinc Titanate (ZIT) glass-ceramic matrix is a promising candidate for immobilizing radioactive Eu element based on the reactions between Eu and raw material of Zn 2 TiO 4 /CaHPO 4 . However, the generated Eu 2 Ti 2 O 7 ceramic with unstable fluorite defect structure hampers the mechanical properties and chemical durability of ZIT-Eu (ZIT glass-ceramic waste form immobilized with Eu 2 O 3 ). This study addresses the issue by developing the Ce-doping ZIT-Eu waste forms using a solid-phase sintering method at high temperature, realizing the conversion from unstable defective fluorite to stable pyrochlore. As evidenced by the experimental measurements, the density, hardness, water absorption and chemical stability are significantly improved after Ce doping, with the 6 wt% Ce exhibiting the best aqueous durability, retaining a leach value of only 0.11 × 10 −9 g·m −2 ·d −1 at 90 ℃ after 28 days. Furthermore, the density functional theory calculation reveals the potential mechanism that Ce doping decreases the adsorption capability for H 2 O and increases the lattice energy for Eu ion diffusion. In conclusion, this simple doping strategy promotes the application of ZIT glass-ceramic matrix in the field of radioactive waste treatment.
研究领域:
核废物固定
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材料科学
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固态化学
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放射化学
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陶瓷材料
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产品关联
DOI
高影响因子
ADVANCED MATERIALS
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2025
·
IF 26.8
Deqi Fan,Chengxiao Zhao,Yi Lu,Guangyao Zhang,Zongze Li,Xiang Wang,Xiaofei Yang
Organic photocatalysts generally suffer from insufficient near-infrared light absorption and undesirable photogenerated charge transport properties, resulting in unfavorable hydrogen evolution performance from water splitting. Hydrogen evolution reaction (HER) is also known to be significantly influenced by the interfacial charge and mass transfer in a catalyst/H 2 O biphase system. Herein, for the first time, a highly stable and floating hydrogen-water cogeneration hybrid hydrogel that utilizes photothermal-induced interface microenvironment variation to accelerate sluggish photocatalytic water splitting reaction is reported. Supported by solar-powered interfacial evaporation and efficient vapor generation, the rationally designed hydrogel effectively transforms the conventional liquid-solid interface into a gas-solid photocatalytic interface. The presence of gas-liquid coexistence state offers a disordered and loose hydrogen-bond network while preserving the proton transfer channel, greatly reducing reaction activation energy and interfacial energy barriers. The improved heat and mass transfer together with optimized charge transfer pathways suppress electron-hole recombination, the integrated photothermal-coupled solar photocatalytic hydrogel exhibits excellent operational stability and self-adaptive rotation in seawater, mitigating salt accumulation and achieving an exceptional vapor generation rate of 4.71 kg m −2 h −1 and a hydrogen-evolving rate of 1961.25 µmol g −1 h −1 under one sun illumination.
研究领域:
材料科学
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能源科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2025
·
IF 7.2
Yinlin Chang,Tao Liu,Jingyuan Rao,Min Fu,Yijun Guo,Shuliang Zou,Dewen Tang,Zetian Tao
The safe immobilization of radiotoxic strontium (Sr) in high-level radioactive waste (HLW) remains a major challenge due to its tendency to segregate and leach under aqueous conditions. In this study, a surface vanadium (V) engineering strategy is proposed to enhance the chemical stability of Sr 2 Fe 1.5 Mo 0.5 O 6-δ (SFM) ceramics. V-doped samples (SFMV), synthesized via a sol-gel method with 0.05 mol V partially substituting Mo, show improved resistance to Sr migration. DFT calculations reveal that V modification increases the Sr migration barrier from 0.14 eV (in SFM) to 0.34 eV (in SFMV) and induces the formation of strong Fe-O-V, and Mo–O–V effectively suppressing Sr surface segregation. Additionally, the formation of SrMoO 4 phases may act as Sr sinks, further enhancing stability. Leaching tests show that the Sr release from SFMV drops from 7.43 × 10 −4 to 2.89 × 10 −6 g·cm −2 ·d −1 over 42 days, representing a 64 % reduction compared to pristine SFM. This work provides a promising strategy for designing durable ceramic hosts for Sr-containing HLW.
研究领域:
材料科学
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核工程
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环境化学
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固态化学
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产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Wenli Xia,Jingcong Xie,Linguo Zhao,Jianjun Pei
Thermophilic enzymes possess numerous favorable properties for biotransformation, including high catalytic activity, exceptional thermal stability, and ease of purification and preparation. However, the challenge arises from the lack of suitable enzyme immobilization materials that exhibit both thermal tolerance and reusability, thereby hindering the effective and recurrent utilization of thermophilic enzymes. In this study, a specially designed dual-network hybrid-gel consisting of acrylamide (AM) and sodium alginate (SA) was developed to serve as a thermotolerant and reusable carrier for the efficient immobilization of a thermotolerant α-rhamnosidase IagRha. The optimal amounts of AM and SA were determined to be 5 % and 2 %, respectively. Following the step of acrylamide crosslinking to establish the skeleton of hybrid-gel, a subsequent immersion in a Ca 2+ solution facilitated the formation of ionic bonds between SA and Ca 2+ , thereby adjusting the internal voids of the hydrogel, which effectively enhanced the thermal stability and enzyme immobilization efficiency of the hybrid-gel. This innovative approach resulted in the achievement of 93.65 % recovery of enzyme activity, along with remarkable enhancements in enzymatic properties. The immobilized α-rhamnosidase, AM-SA-IagRha, demonstrated a significant 1.34-fold increase in the Vmax value, rising from 5.28 U/mg to 7.06 U/mg, compared to free IagRha. Furthermore, AM-SA-IagRha exhibited excellent thermostability, with a half-life of 99.0 min at 90 °C, which was 1.57 times higher than that of free IagRha. After four cycles of reuse, the productivities of rutin and epmdin C by the immobilized enzyme were 1.91 and 2.53 times that of the free enzyme, respectively. Additionally, the enzyme activity of AM-SA-IagRha remained at 90.16 % after storage at 4 °C for 30 days, and retained at 85.63 % after 20 cycles of reuse, demonstrating exceptional storage stability and reusability. The optimal expression conditions for IagRha were identified in TB medium incubated at 37 °C without IPTG, resulting in an enzyme activity of 6.32 U/mL. These significant findings underscore the potential of AM-SA-IagRha for industrial applications, particularly in high-temperature biotransformation.
研究领域:
生物技术
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酶工程
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生物化学
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应用微生物学
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工业生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）