JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Xinmiao Wang,Qian Wang,Xu Guo,Peiyong Tong,Chuan Li,Dayong Zhou,Fawen Yin
BACKGROUND Phenolic compounds are increasingly recognized for their diverse health benefits, particularly their potent antioxidant capacities. To enhance its lipophilicity, stability and bioavailability, hydroxytyrosol has been successfully esterified with various alkyl chains lengths and different isomers (branched-chain and straight-chain), forming hydroxytyrosol-fatty acid acyl esters (Hty-Es). These synthesized Hty-Es, have garnered significant interest and are widely explored in fields such as food preservation because of their superior antioxidant properties. However, little is known about the digestion, fermentation characteristics and the effects on the intestinal microbiota in vitro . RESULTS This study evaluated the influence of hydroxytyrosol (HTy) esterified with fatty acids of varying chain lengths (C2:0, C3:0, C4:0 n , C4:0 i , C6:0, C8:0, C12:0, C14:0 and C16:0) on polyphenol sustained-release properties and gut microbiota modulation during simulated in vitro digestion, fecal fermentation and Lactobacillus fermentation. The findings demonstrated partial hydrolysis of HTy-Es in both simulated gastric fluid and intestinal fluid to slowly release a small amount of polyphenols, suggesting their potential to reach the colon and cecum. Analysis of fermented fecal samples revealed that HTy-Es supplementation modulated microbiota composition, elevating the diversity of Actinobacteriota, Firmicutes and Bacteroidetes , whereas it decreased the diversity of Desulfobacterota, Actinobacteriota, Chloroflexi and Verrucomicrobiota . At the genus level, the growth of detrimental genera ( Escherichia shigella ) was successfully suppressed. Notably, HTy-Es with distinct acyl chain lengths and isomer configurations exhibited divergent effects on polyphenol bioaccessibility and gut microbiome diversity, underscoring their structure-dependent functional potential for health applications. CONCLUSION Conclusively, the slow release of fatty acids and HTy clearly demonstrated that HTy-Es would be an effective approach for enhancing the beneficial health effects of fatty acids and HTy. © 2025 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2025
·
IF 5.4
Shiqi Shen,Cailing Tong,Yujia Ou,Tao Hong,Zhipeng Li,Yanbing Zhu,Hui Ni,Zedong Jiang,Mingjing Zheng
The digestive fermentation characteristics of κ-carrageenan with different molecular weights and its acetylated derivatives were investigated through the changing rules of intestinal flora and metabolites. The results showed that κ-carrageenan with different molecular weights and its acetylated derivatives did not digested in the oralgastric-small intestinal digestion process but could be fermented by colonic intestinal flora, and was mainly degraded in the ascending colon stage. The lower molecular weight κ-carrageenan was more likely to increase the abundance of Proteobacteria and Enterobacteriaceae, decreased colonic acetic acid production at transverse-descending colon, which could be reversed by the acetylation modification of κ-carrageenan.Meanwhile, the acetylation modification of κ-carrageenan increased the abundance of beneficial bacteria such as Lactobacillus and Bifidobacterium, as well as greatly increased the contents of acetic acid. Moreover, the increased acetic acid production was involved in the up-regulation of acetylated κ-carrageenan on the expression acetylcoenzyme A, acetate kinase, and formate dehydrogenase through the Wood-Ljungdahl pathway. The results indicated that acetylation modification can ameliorate the potential intestinal dysbiosis caused by κ-carrageenan especially with low-molecularweight by increasing beneficial gut microbiota e.g., Lactobacillus and acetic acid metabolism, which can provide precise molecular evidence for the functional optimization of κ-carrageenan and its application in intestinal health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Mengzhen Jia,Xinyu Hu,Fangjie Ning,Kexin Geng,Tian Yuan,Zhigang Liu
Low-fiber diet poses a health risk, yet mechanisms linking dietary fiber deprivation to emotional disorders are unclear. Here, we demonstrate that dietary fiber deprivation (FD) induces pronounced anxiety-like behaviors in mice by disrupting the gut-brain axis. In the FD model, a 6-week intervention with mannan oligosaccharides (MOS, 500 mg/kg) ameliorated these deficits. Mechanistically, FD disrupted gut microbiota and impaired gut barrier integrity. FD triggered colonic inflammation, oxidative stress, and inhibited mitochondrial oxidative phosphorylation─a critical aspect of colonic energy metabolism. These intestinal insults were associated with hyperactivation of the HPA axis and neuroinflammation in the brain. MOS reversed these effects by reshaping the gut microbiota, promoting short-chain fatty acids (SCFAs), and restoring colonic energy homeostasis. Colonic energy metabolism is a critical node in the gut-brain axis, linking fiber intake to emotional regulation. Therefore, dietary fiber deprivation affects the HPA axis and emotions, making it an important nutritional intervention target.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yan Gao,Lina Qiao,Junyi Wang,Hui Zhang,Chunhui Huang,Shengbao Feng,Huijuan Li,Ning Zhang,Fuping Zheng,Mingquan Huang
Qingke baijiu (QKJ), also known as Highland barley wine, is a traditional Chinese fermented baijiu from the Tibetan Plateau. It is a kind of light-aroma baijiu (LAJ) with a unique flavor distinct from other LAJs produced using Daqu . In this work, QKJ and other LAJs ( Daqu ) were selected for comparison of their aroma differences using a molecular sensory approach. In total, 66 aroma compounds in QKJ and 73 in LAJ were identified via gas chromatography-olfactometry-mass spectrometry (GC-O-MS). After quantification, odor activity values (OAVs) calculation and orthogonal partial least squares discriminant analysis (OPLS-DA), ethyl butyrate , ethyl hexanoate , furfuryl alcohol and ethyl isobutyrate were recognized as the major aroma compounds distinguishing QKJ from LAJ. Further recombination and omission experiments revealed ethyl hexanoate, ethyl valerate , γ -nonanolactone, ethyl acetate and benzeneacetaldehyde diethyl acetal as critical aroma components in QKJ. This work provides valuable insights into the flavor and quality control of Chinese baijiu.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR NEUROBIOLOGY
·
2025
·
IF 4.3
Li Ting,Zhou Sheng-Yang,Quan Wei,Zhou Yu,Wu Jian,Zhao Li-Ping,Qiao Chen-Meng,Zhao Wei-Jiang,Cui Chun,Shen Yan-Qin
More than 80% of patients with Parkinson’s disease (PD) exhibit severe gastrointestinal symptoms, which seriously affect life quality of life. Several large-scale epidemiological studies have confirmed that exercise can improve motor symptoms of PD patients, but the influence on gastrointestinal symptoms has not been reported. To clarify whether exercise can enhance intestinal barrier and ameliorate intestinal inflammation in PD mice, we examined whether moderate-intensity treadmill exercise for 6 weeks could alleviate gastrointestinal symptoms in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced subacute PD mice. Our results demonstrated that 6 weeks moderate-intensity treadmill exercise enhanced the expression of Muc-2 and tight junction proteins, while reducing the expression of cytokines and chemokines, in the distal colon. Moreover, 6 weeks of treadmill exercise reduced the concentrations of short-chain fatty acids (SCFAs) and the expression of G protein-coupled receptor 43 (GPR43) to maintain the intestinal barrier and alleviate intestinal inflammation. Further data showed that 6 weeks of moderate-intensity treadmill exercise enhanced insulin-like growth factor 1 (IGF-1) expression and activated the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathway. Taken as a whole, these results revealed that 6 weeks of moderate-intensity treadmill exercise enhanced intestinal barrier function, reduced intestinal inflammation and corrected abnormal concentrations of SCFAs by activating IGF-1/PI3K/Akt pathway in MPTP-induced subacute PD mice.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Qianyun Zhao,Yubin Liu,Mingxing Li,Long Zhao,Tiangang Wang,Yaqin Xiao,Shulin Wei,Ke Wu,Jiayue Yang,Yaqiu Wang,Yueshui Zhao,Fukuan Du,Yu Chen,Shuai Deng,Jing Shen,Zhangang Xiao,Zhi Li,Shengpeng Wang,Xu Wu
Hawthorn fruit is rich in pectins; however, the gut microbiota-modulating effects of hawthorn pectin remain unclear. In this study, enzyme-extracted hawthorn pectin ( M w : 126.24 kDa; HG/RG-I ratio: 55.64 %/37.93 %; branching degree: 2.44) was fractionated into two components: D1 (spherical, 345.76 kDa, HG/RG-I 57.48 %/37.25 %, branching degree 2.16) and D2 (flexible, 17.20 kDa, HG/RG-I 78.24 %/21.76 %, branching degree 1.33). Hawthorn pectin directly stimulated Dubosiella newyorkensis and Bifidobacterium bifidum growth in vitro . In hyperlipidemic mice, it alleviated dyslipidemia and enriched Lactobacillus and Dubosiella . Parallel experiments confirmed that D. newyorkensis supplementation similarly reduced dyslipidemia and enhanced Lactobacillus abundance, mediated by bacterial acetate production. Only D1 mimicked the pectin's prebiotic activity, promoting D. newyorkensis and B. bifidum growth. The high-RG-I D1, with elevated branching and larger M w , was identified as the key driver of hawthorn pectin's prebiotic effect. These findings highlight that hawthorn pectin and D1 specifically target probiotic D. newyorkensis to synergize with Lactobacillus against hyperlipidemia.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xingfang Xie,Yao Gu,Yi Liu,Mingyue Shen,Jiaojiao Ji,Jianping Gao,Jiankuan Li
In the present study, an inulin-type fructan (ITF) with the degree polymerization (DP) of 21 was isolated from Codonopsis pilosula roots and its structure was characterized by FT-IR, MALDI-TOF-MS and NMR. The immunomodulatory and intestinal protective effects of ITF were investigated on immunosuppressive mice. Male BALB/c mice were pretreated with cyclophosphamide (Cy) for 3 days to establish an immunosuppressive model followed by ITF treatment. The results demonstrated that compared with the model group, ITF administration significantly increased immune organ index ( P <0.05), alleviated intestinal villus damage, stimulated serum cytokine secretion including Ig G, IL-4, IL-6, IL-2, TNF-α, and INF-γ ( P <0.05), upregulated the expression of Occludin and Claudin-1 ( P <0.05), and increased CD4 + and CD8 + T cells of ileum in Cy-induced mice ( P <0.05). Furthermore, ITF restored the intestinal microbiota dysbiosis caused by Cy by increasing the abundance of Muribaculaceae , Blautia , Odoribacter , Lactobacillus and decreasing the abundance of Lachnospiraceae _ NK4A136 _ group ( P <0.05). Meanwhile, ITF increased the production of short-chain fatty acids (SCFAs) including acetic acid, propionic acid and butyric acid ( P <0.05). These results indicated that ITF can ameliorate cyclophosphamide-induced immunosuppression and intestinal barrier injury, and restore gut microbiota dysbiosis. This study provided important evidences for the immunomodulatory and intestinal protective effects of the ITF from C. pilosula .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hongru Yang,Chongbin Lei,Dongyao Li,Lei Ma,Na Zhang,Yumiao Lang,Liping Wu,Miaoshu Wang,Hongtao Tian,Chen Li
Moringa oleifera Lamarck seeds (MOS) have been traditionally used in folk medicine and documented for their potential to alleviate type 2 diabetes symptoms, but the potential mechanisms are still unknown. The purpose of this article is to investigate the effects of MSAP (alkali-extracted polysaccharide from MOS) on diabetic rats by assessing its impact on the gut microbiome, diabetes-related biochemical markers, and fecal metabolomics. The results demonstrated that the fasting blood glucose, glucose tolerance, insulin tolerance, insulin level and lipopolysaccharides (LPS) level in the rats treated with MSAP were all improved. Specifically, MSAP was found to modulate the composition and diversity of the gut microbiota, increasing the ratio of Firmicutes / Bacteroidetes , which enhanced the quantity of probiotic Lactobacillus and butyrate-producing bacteria, such as Roseburia , thereby reinforcing the intestinal epithelial barrier. Furthermore, fecal metabolomics indicates that MSAP actively regulates pathways closely associated with diabetes, including sphingolipid metabolism, amino acid synthesis and catabolism, retrograde endogenous cannabinoid signaling, and the modulation of TRP channels by inflammatory mediators. By integrating microbiome and metabolomics data, this study elucidated the mechanisms through which MSAP alleviates diabetes. In conclusion, the findings suggest that polysaccharides from MOS hold potential as a medicinal and edible homologous food for diabetes management.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
You Luo,Ruling Tang,Yongguang Huang
Red raspberries are associated with various health benefits, with pectic polysaccharides as their primary component and potential key contributor to these effects. This study aimed to evaluate the antioxidant and prebiotic potential of four red raspberry pectic polysaccharides (RP)—EN-RP (enzyme-assisted extraction), AC-RP (acid-assisted extraction), AL-RP (alkali-assisted extraction), and US-RP (ultrasound-assisted extraction)—and to elucidate the relationship between their structure and function. AC-RP and US-RP contained higher proportions of homogalacturonan (HG) at 50.92 % and 53.10 %, respectively, while EN-RP and AL-RP exhibited higher proportions of rhamnogalacturonan-I (RG-I) at 63.89 % and 43.37 %, respectively. All four polysaccharides demonstrated significant antioxidant and prebiotic properties. AL-RP exhibited the strongest DPPH radical scavenging activity, while US-RP showed the highest hydroxyl radical scavenging ability. These pectic polysaccharides were highly fermentable, significantly modulating gut microbiota composition and promoting the production of propionic acid, particularly EN-RP and AL-RP. Compared to the blank group, RP intervention significantly enriched Bacteroides , Phocaeicola , Bifidobacterium , Limosilactobacillus , and Paraprevotella . Carbohydrate-active enzyme genes in metagenomes revealed that glycoside hydrolases played a vital role in the degradation and utilization of red raspberry polysaccharides. Furthermore, correlation analysis indicated that a higher RG-I proportion and an elevated Rha/GalA ratio enhanced the abundance of certain beneficial microbial species and increased propionic acid production. These findings advance the understanding of the structure-function relationship of natural pectic polysaccharides and highlight their potential for tailoring gut microbiota and promoting health through precise dietary interventions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INFLAMMATION
·
2012
·Vol. 35
·期 5
·1676-1684
·
IF 1.747
Liu Tengfei,Li Jing,Liu Yuxin,Xiao Nan,Suo Haitao,Xie Kun,Yang Chunliu,Wu Chen
Short-chain fatty acids (SCFAs) produced by the colonic bacterial fermentation of dietary fiber contribute a significant proportion of daily energy requirement. Furthermore, these compounds are modulators of macrophage function and potential targets for the development of new drugs. The aims of this study were to evaluate the effects of three types of SCFAs (sodium acetate (NaAc), sodium propionate (NaP), and sodium butyrate (NaB)) on the production of NO and inducible nitric oxide synthase (iNOS) and proinflammatory and antiinflammatory cytokines (tumor necrosis factor-α (TNF-α) and interleukin (IL-1, IL-6, and IL-10)) and to observe the effect of NaAc on inhibiting lipopolysaccharide (LPS)-induced NF-κB activation in LPS-stimulated RAW264.7 cells. The results show that three types of SCFAs (acetate, propionate, and butyrate) reduced the production of proinflammatory factors, including TNF-α, IL-1β, IL-6, and NO, and inhibited the vitality of iNOS. Meanwhile, SCFAs enhanced the production of antiinflammatory cytokine IL-10 in lower concentrations (1–1,200 μmol/L). Like NaB, NaAC inhibited LPS-induced NF-κB activation. These results may hold promise on the role that SCFAs have on the prevention and treatment of various inflammatory conditions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 8
·1300
·
IF 5.1
Tingting Bu,Yue Yu,Xiao Kong,Weicheng Wu,Zhiguo Zhang,Weiwei Hu,Komarova Natallia,Ming Cai,Kai Yang,Peilong Sun
The effects of cellulase–xylanase synergistic treatment combined with twin-screw extrusion on the physicochemical, functional, and in vitro fermentation characteristics of buckwheat bran dietary fiber (BBDF) were investigated. Compared to single enzymatic hydrolysis, the synergetic modification was more effective in promoting the soluble DF (SDF) ratio (increased from 10.68% to 32.67%), functional properties, and prebiotic activities of BBDF and decreasing the insoluble DF (IDF) content. Under 0.6% (w/w) cellulase and xylanase with mild extrusion conditions (40–80 °C), the modified BBDF exhibited the highest capacities for glucose and cholesterol adsorption. FTIR and XRD experiments indicated that the enzymatic extrusion destroyed the intermolecular interactions of BBDF. Furthermore, enzymatically extruded BBDFs showed 2.2-fold higher short-chain fatty acid (SCFA) yields during in vitro fecal fermentation (total SCFAs: 87.8 mM vs. 40.0 mM in control), with butyrate production reaching 2.5 mM (+76.3%), among which the mildly extruded BBDFs exhibited superior prebiotic effects.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Juan Li,Zhaozhan Liu,Yan Du,Zhiming Zhong,Zhaoxin Tu,Wenju Zhou,Zhengxing Chen,Li Wang,Xinxia Zhang,Ting Li
Alcoholic liver injury is a prevalent health issue worldwide, characterized by oxidative stress, inflammation, and gut microbiota imbalance. Traditional treatments often focus on reducing alcohol intake and managing symptoms, but novel therapeutic approaches targeting gut microbiota and oxidative stress are gaining attention. Highland barley functional tea (HBFT) extract was known for its antioxidant and anti-inflammatory properties, but its effects on alcoholic liver injury and gut microbiota remained unclear. This study aimed to evaluate the protective effects of HBFT on alcoholic liver injury and elucidated its underlying mechanisms of action. Male C57BL/6J mice were divided into six groups (n=12): control, model (0.2 mL/20 g per day), positive drug (0.2 mL/20 g per day), low-dose HBFT (1.4 g/kg per day), medium-dose HBFT (2.8 g/kg per day), and high-dose HBFT (5.6 g/kg per day). The findings indicated that HBFT intervention significantly inhibited the increase in ALT and AST serum levels. HBFT also enhanced the activity of enzymes and expression of proteins associated with oxidative stress, inflammatory damage, and hepatic lipid metabolism. Furthermore, HBFT promoted the proliferation of beneficial gut bacteria in mice, such as Akkermansia and Lactobacillus. Moreover, the HBFT intervention helped restore the levels of short-chain fatty acids (SCFAs) in mice caused by alcohol exposure. Taken together, these results suggested that HBFT extract may act as a novel gut microbiota modulator, alleviating alcoholic liver injury by restoring gut microbial balance and ameliorating hepatic oxidative stress and inflammation.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）