INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Min Hu,Jia-Xu Song,Shi-Tao Miao,Cheng-Kai Wu,Xing-Wen Gong,Hong-Ju Sun
Acetaldehyde dehydrogenase 2 (ALDH2) is a crucial enzyme in alcohol metabolism, and oral administration of ALDH2 is a promising method for alcohol detoxification. However, recombinant ALDH2 is susceptible to hydrolysis by digestive enzymes in the gastrointestinal tract and is expressed as inactive inclusion bodies in E. coli . In this study, we performed three rounds of rational design to address these issues. Specifically, the surface digestive sites of pepsin and trypsin were replaced with other polar amino acids, while hydrophobic amino acids were incorporated to reshape the catalytic cavity of ALDH2. The resulting mutant DE2–852 exhibited a 45-fold increase in soluble expression levels, while its stability against trypsin and pepsin increased by eightfold and twofold, respectively. Its catalytic efficiency ( k cat /K m ) at pH 7.2 and 3.2 improved by more than four and five times, respectively, with increased V max and decreased K m values. The enhanced properties of DE2–852 were attributed to the D457Y mutation, which created a more compact protein structure and facilitated a faster collision between the substrate and catalytic residues. These results laid the foundation for the oral administration and mass preparation of highly active ALDH2 and offered insights into the oral application of other proteins.
研究领域:
生物化学
·
蛋白质工程
·
药物开发
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Longsheng Zhang,Qianwei Ding,Yunpeng Huang,Huahao Gu,Yue-E Miao,Tianxi Liu
The practical applications of transition metal oxides and hydroxides for supercapacitors are restricted by their intrinsic poor conductivity, large volumetric expansion, and rapid capacitance fading upon cycling, which can be solved by optimizing these materials to nanostructures and confining them within conductive carbonaceous frameworks. In this work, flexible hybrid membranes with ultrathin Ni(OH)2 nanoplatelets vertically and uniformly anchored on the electrospun carbon nanofibers (CNF) have been facilely prepared as electrode materials for supercapacitors. The Ni(OH)2/CNF hybrid membranes with three-dimensional macroporous architectures as well as hierarchical nanostructures can provide open and continuous channels for rapid diffusion of electrolyte to access the electrochemically active Ni(OH)2 nanoplatelets. Moreover, the carbon nanofiber can act both as a conductive core to provide efficient transport of electrons for fast Faradaic redox reactions of the Ni(OH)2 sheath, and as a buffering matrix to mitigate the local volumetric expansion/contraction upon long-term cycling. As a consequence, the optimized Ni(OH)2/CNF hybrid membrane exhibits a high specific capacitance of 2523 F g–1 (based on the mass of Ni(OH)2, that is 701 F g–1 based on the total mass) at a scan rate of 5 mV s–1. The Ni(OH)2/CNF hybrid membranes with high mechanical flexibility, superior electrical conductivity, and remarkably improved electrochemical capacitance are condsidered as promising flexible electrode materials for high-performance supercapacitors.
研究领域:
材料科学
·
能源存储与转换
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF THERMOPHYSICS
·
2026
·Vol. 47
·期 8
·141
·
IF 3.6
Li Yan,Tian Lantu,Wang Shuo,Zhu Qunzhi
As the key heat storage and transfer medium in concentrated solar power (CSP) systems, molten salts’ thermophysical properties, such as thermal conductivity, specific heat capacity, and thermal stability, critically determine the systems energy conversion efficiency and economic viability. However, conventional molten salts suffer from low thermal conductivity and limited energy storage density, restricting their application in high-temperature thermal energy storage. Recent advances show that doping with nanoparticles, such as Al 2 O 3 , CuO, and SiO 2 , can significantly improve these properties. Nevertheless, systematic studies on quaternary molten salt systems remain scarce. In this work, we focus on the quaternary eutectic salt H15 (15 wt% Ca(NO 3 ) 2 –5.95 wt% NaNO 3 –45.05 wt% KNO 3 –34 wt% NaNO 2 ), which demonstrates a high decomposition temperature of 597.4 °C alongside a relatively low melting point of 84.4 °C and high thermal energy storage density of 1052.41 kJ/kg. To overcome its thermal performance limitations, Al 2 O 3 nanoparticles (0.1 wt% to 3 wt%) were introduced to form a nanocomposite molten salt. The influence of varying nanoparticle loadings was assessed via DSC, HotDisk, SEM, and XRD to examine corresponding changes in phase transition behavior, thermal conductivity, viscosity, and material microstructure. This study provides theoretical and experimental support for developing high-performance, wide temperature-range thermal energy storage materials for advanced CSP systems.
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2026
·
IF 3.9
Liao Xuemei,Zhuang Ruirong,Zhuang Tangting,Xie Meijiao,Zhou Beidou
In this study, 45 2-benzylidene-1-indolone compounds were synthesized and their antitumor activities were evaluated. Experimental results demonstrated that these compounds exhibited varying degrees of inhibition against cancer cell proliferation. Notably, compound 20 showed significant activity against HGC-27 gastric cancer cells, with a half-maximal inhibitory concentration (IC 50 ) as low as 2.57 µM. Finally, the antitumor mechanism of compound 20 was investigated through network pharmacology and molecular docking, and 12 key genes and 21 related tumor signaling pathways were obtained. These findings suggest that compound 20 , as a potential lead compound, may provide a new direction for the development of antitumor drugs after structural optimization.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Caiyun Wang,Xingxing Wu,Yufan Xia,Xuan Zhang,Hongge Pan,Mi Yan,Yong Li,Yinzhu Jiang
Mg metal anodes experience significant passivation of harmful side reactions in conventional electrolyte consisting of magnesium bis(trifluoromethane sulfonyl)imide (Mg(TFSI) 2 ) in 1,2-dimethoxyethane (DME), which critically impedes the advancement of high-performance rechargeable magnesium batteries. Herein, an innovative electrolyte system comprising an ultra-dilute 0.02 M Mg(TFSI) 2 in DME with 4-chlorobutyl methyl ether (CME) as co-solvent is strategically devised. This unique formulation effectively suppresses the formation of TFSI − -derived passivation byproducts while simultaneously facilitating the establishment of protective Cl-rich interphase, thereby achieving exceptional reversibility in Mg plating/stripping processes. Remarkably, Mg electrodes demonstrate stable cycling performance with a minimal overpotential of 70 mV at 0.1 mA cm −2 in the optimized 0.02 M Mg(TFSI) 2 -DME/CME electrolyte. More impressively, the system maintains reversible electrochemical operation for 400 h even at 2 mA cm −2 . This work presents a novel electrolyte formulation for overcoming the limitations of Mg metal anode in conventional Mg(TFSI) 2 based electrolytes, offering new perspectives for the development of next-generation rechargeable magnesium batteries.
研究领域:
电化学
·
能源材料
·
电池技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Li-Xia Zhao,Wei Hu,Zi-Bin Jiang,Jia-Yu Wang,Kui Wang,Shuang Gao,Ying Fu,Fei Ye
4-Hydroxyphenylpyruvate dioxygenase inhibitors (Echinochloa crus-galli 1.13.11.27, HPPD) have gained significant popularity as one of the best-selling herbicides worldwide. To identify highly effective HPPD inhibitors, a rational design approach utilizing bioisosterism was employed to create a series of 2-(arylformyl)cyclohexane-1,3-dione derivatives. A total of 29 novel compounds were synthesized and characterized through various techniques, including IR, 1H NMR, 13C NMR, and HRMS. Evaluation of their inhibitory activity against Arabidopsis thaliana HPPD (AtHPPD) revealed that certain derivatives exhibited superior potency compared to mesotrione (IC50 = 0.204 μM). Initial herbicidal activity tests demonstrated that compounds 27 and 28 were comparable to mesotrione in terms of weed control and crop safety, with compound 28 exhibiting enhanced safety in canola crops. Molecular docking analyses indicated that the quinoline rings of compounds 27 and 28 formed more stable π–π interactions with the amino acid residues Phe-360 and Phe-403 in the active cavity of AtHPPD, surpassing the benzene ring of mesotrione. Molecular dynamics simulations and molecular structure comparisons confirmed the robust binding capabilities of compounds 27 and 28 to AtHPPD. This study provides a valuable reference for the development of novel triketone herbicide structures, serving as a blueprint for future advancements in this field.
研究领域:
植物学
·
药理学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2014
·
IF 2.468
Wei Wei,Yongfang Feng,Xi Zhang,Xi Cao,Fengqin Feng
To obtain high purity of 1,3-Dioleoyl-2-palmitoylglycerol (OPO), an important structured triglyceride for infant formula, a facile method was established. Tripalmitin (PPP) was firstly synthesized, and then OA was incorporated into the sn -1 and 3 position of the PPP glycerol backbone by acidolysis catalyzed using sn -1,3 regiospecific lipases. Two kinds of widely used immobilized sn -1,3 regiospecific lipases, Lipozyme TL IM and Lipozyme RM IM, were investigated in both solvent ( n -hexane) and solvent-free system. The results showed that Lipozyme TL IM and Lipozyme RM IM were suitable for solvent system and solvent-free system respectively. Other reaction conditions (enzyme load, substrate molar ratio, temperature, reaction time) were optimized. The palmitic acid (PA) at the sn -2 position of the obtained triacylglycerols (TAGs) under optimized conditions were 92.92% and 86.62%, and OPO contents in the final products were 32.34% and 40.23% in the solvent and solvent-free system respectively. Special emphasis was given to test the reusability of the two immobilized lipases, results showed that Lipozyme TL IM was almost non-reusable, while Lipozyme RM IM could keep its activity for at least 15 times (about 60 h). These results provide a guide for the industrial production of high purity OPO by facile method.
研究领域:
食品科学
·
生物化学
·
酶工程
✓ 已验证
DOI
高影响因子
Journal of the American Chemical Society
·
2017
·
IF 13.858
Yuyang Zhang,Yi Zou,Nelson L. Brock,Tingting Huang,Yingxia Lan,Xiaozheng Wang,Zixin Deng,Yi Tang,Shuangjun Lin
3-Substituted 2-oxindoles are important structural motifs found in many biologically active natural products and pharmaceutical lead compounds. Here, we report an enzymatic formation of the 3-substituted 2-oxindoles catalyzed by MarE in the maremycin biosynthetic pathway in Streptomyces sp. B9173. MarE is a homologue of FeII/heme-dependent tryptophan 2,3-dioxygenases (TDOs). Typical TDOs usually catalyze the insertion of two oxygen atoms from O2 into an indole ring to generate N-formylkynurenine (NFK)-like products. In contrast, MarE catalyzes the insertion of a single oxygen atom from O2 into an indole ring, to probably generate an epoxyindole intermediate that undergoes an unprecedented 2,3-hydride migration to form 2-oxindole structure. MarE shows substrate robustness to catalyze the conversion of a series of 3-substituted indoles into their corresponding 3-substituted 2-oxindoles. Although containing most key amino acid residues conserved in well-known TDO homologues, MarE falls into a separate new subgroup in the phylogenetic tree. The characterization of MarE and its homologue enriches the functional diversities of TDO superfamily and provides a new strategy for discovering novel natural products containing 3-substituted 2-oxindole pharmacophores by genome mining.
研究领域:
生物化学
·
微生物学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 10
·5715
·
IF 6.208
He Wang,Jingjing Li,Qian Yang,Lan Wang,Jing Wang,Yaxin Zhang,Yanjing Guo,Rui Li,Ruiqi Zhang,Xiaorong Tao,Bernal E. Valverde,Sheng Qiang,Hazem M. Kalaji,Shiguo Chen
2-Amino-3-methylhexanoic acid (AMHA) was synthetized as a non-natural amino acid more than 70 years ago; however, its possible function as an inducer of plant resistance has not been reported. Plant resistance inducers, also known as plant elicitors, are becoming a novel and important development direction in crop protection and pest management. We found that free AMHA accumulated in the mycelia but not in fermentation broths of four fungal species,Magnaporthe oryzaeand threeAlternariaspp. We unequivocally confirmed that AMHA is a naturally occurring endogenous (2S, 3S)-α-amino acid, based on isolation, purification and structural analyses. Further experiments demonstrated that AMHA has potent activity-enhancing resistance against extreme temperature stresses in several plant species. It is also highly active against fungal, bacterial and viral diseases by inducing plant resistance. AMHA pretreatment strongly protected wheat against powdery mildew,ArabidopsisagainstPseudomonas syringaeDC3000 and tobacco againstTomato spotted wilt virus. AMHA exhibits a great potential to become a unique natural elicitor protecting plants against biotic and abiotic stresses.Keywords:agricultural production;natural product;plant resistance inducer;biopesticide;biotic and abiotic stress
研究领域:
植物学
·
农业科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Chuan-Jun Yue,Xin Sun,Han Xiao,Xin-Yan Jin
In the framework of sustainable development, researchers in both traditional and modern chemistry are compelled to pursue innovation. The inherent drawbacks of conventional ester synthesis methods highlight the urgent need to develop efficient and environmentally benign approaches. This study introduces an electrochemical-intensified heterogeneous catalytic strategy for acrylate synthesis. A distinctive heterogeneous catalyst has been developed by bonding 2-methoxyphenothiazine onto nanosilica surface through the coupling agent 3-chloropropyltrimethoxysilane. The characterization of the catalyst was conducted through Infrared spectroscopy (IR), X-ray diffraction (XRD), scanning electron microscope (SEM), and thermogravimetry (TG), as well as the Kjeldahl method, revealing a 12.86% loading and uniform distribution. The catalyst demonstrated remarkable efficacy in facilitating the esterification of acrylic acid and monohydric alcohols at room temperature, uniformly varying reaction rate processes compared to acid catalysis and achieving a catalytic yield exceeding 85%. Moreover, the catalytic activity largely remained consistent over six cycles. The electrochemical enhancement was instrumental in modifying the traditional esterification process. The electrochemical-intensified heterogeneous catalytic system for the esterification of acrylic acid meets the standards for green production. This research provides novel insights into integrated catalytic technologies for esterification, contributing to the advancement of sustainable chemistry.
研究领域:
催化化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
Food & Function
·
2025
·
IF 5.4
Mengzhen Jia,Xiaorui Wang,Fangjie Ning,Wenxiu Wang,Xinyu Hu,Kexin Geng,Junqing Wen,Shan Wu,Bin Wang,Zhigang Liu
Alzheimer's disease (AD) is mainly manifested by cognitive dysfunction, accompanied by excessive β-amyloid (Aβ) deposition and neuroinflammation. The regulation of vagus nerve (VN) signal transmission is crucial for influencing the pathological progression of AD and exploring new therapeutic approaches. Two doses of 2'-FL (500 &1000 mg/kg) were administered orally to AD model mice. The 2'-FL rescued spatial and recognition memory deficits in AD mice. Moreover, 2'-FL reduced Aβ deposition and neuroinflammation. The 2'-FL enhances c-Fos expression in the NTS. This suggested that 2'-FL alleviated AD related cognition by enhancing VN afferent activity. Vagotomy demonstrated that the alleviation of AD related cognition by 2'-FL depended on the presence of VN. Moreover, 2'-FL enhanced gut barrier function, alleviated gut inflammatory. Notably, 2'-FL also reshaped the gut microbiota composition, increasing the relative abundance of Intestinimonas, Muribaculum, and others. Moreover, 2'-FL promoted the production of SCFAs. Correlation analysis showed that propionate was highly correlated with other related indicators. After vagotomy, although 2'-FL promoted the SCFAs production, it didn't alleviate cognitive impairment. This suggested that the neuroprotective effect of SCFAs could also be partially dependent on the VN. 2'-FL rescued the cognitive deficits in AD mice, which was partly explained by changes in gut microbial composition, production of SCFAs, and the presence of VN.
研究领域:
神经科学
·
医学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Jia Fang,Jun Li,Long Gao,Xiwang Jiang,Jingwen Zhang,Aihua Xu,Xiaoxia Li
The octahedral molecular sieve manganese oxide (OMS-2) and graphite (Gt) composites (OMS-2/Gt) were prepared by a facile refluxing approach. The structure and morphology of the obtained materials were systematically investigated. The results reveal that OMS-2 nanofibers are uniformly dispersed on the surface of Gt, and the ratio of Mn 3+ species in OMS-2 increases lineally with the increase of Gt dosage. The catalytic activity of OMS-2/Gt was evaluated by the degradation of Acid Orange 7 (AO7) in the presence of peroxymonosulfate (PMS). It is found that the OMS-2/Gt composites show enhanced catalytic performance with the degradation rate lineally correlated with the content of OMS-2 in the catalyst, and even with the ratio of Mn 3+ /Mn 4+ . XPS and radical scavenger experiments further indicated that the oxidation of Mn 3+ by PMS system occurs in the system with the formation of hydroxyl radicals contributed to the dye degradation . The catalysts also exhibit good stability and reusability during consecutive cycles. Thus, the environmental friendly OMS-2/Gt composites with low cost, facile synthesis process and high efficiency are very promising catalysts for PMS activation and pollutants degradation.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）