Brazilian Journal of Otorhinolaryngology
·
2019
·Vol. 85
·期 6
·705-715
·
IF 1.603
Chen Jing,Li Hai Liang,Li Bo Bo,Li Wei,Ma Dong,Li Yong He,Liu Tao
Introduction: Serum- and glucocorticoid-inducible kinase 3, a serine/threonine kinase that functions downstream of the PI3K signaling pathway, plays a critical role in neoplastic processes. It is expressed by various tumors and contributes to carcinogenesis. Objective: The objective was to investigate serum- and glucocorticoid-inducible kinase 3 expression in nasopharyngeal carcinoma, to study the anti-tumor effects of serum- and glucocorticoid-inducible kinase 3 shRNA by inhibiting its expression in nasopharyngeal carcinoma cells and to discuss the potential implications of our findings. Methods: Serum- and glucocorticoid-inducible kinase 3 protein expression in nasopharyngeal carcinoma cell lines (CNE-1, CNE-2, HNE-1, HONE-1, and SUNE-1) and the human immortalized nasopharyngeal epithelium cell line NP69 were assayed by western blotting. Serum- and glucocorticoid-inducible kinase 3 expression in 42 paraffin-embedded nasopharyngeal carcinoma tissues were performed by immunohistochemistry. MTT assay, flow cytometry, and scratch tests were performed after CNE-2 cells were transfected with the best serum- and glucocorticoid-inducible kinase 3 shRNA plasmid selected by western blotting using lipofectamine to study its effect on cell proliferation, apoptosis, and migration. Results: Serum- and glucocorticoid-inducible kinase 3 was overexpressed in human nasopharyngeal carcinoma tissues and cells. Serum- and glucocorticoid-inducible kinase 3 expression decreased markedly after CNE-2 cells were transfected with the serum- and glucocorticoid-inducible kinase 3 shRNA, leading to strong inhibition of cell proliferation and migration. In addition, the apoptosis rate increased in CNE-2 cells after serum- and glucocorticoid-inducible kinase 3 knockdown. Conclusion: Serum- and glucocorticoid-inducible kinase 3 expression was more frequently observed as the nasopharyngeal epithelium progresses from normal tissue to carcinoma. This suggests that serum- and glucocorticoid-inducible kinase 3 contributes to the multistep process of NPC carcinogenesis. Serum- and glucocorticoid-inducible kinase 3 represents a target for nasopharyngeal carcinoma therapy, and a basis exists for the further investigation of this adjuvant treatment modality for nasopharyngeal carcinoma.
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Surfaces and Interfaces
·
2022
·
IF 6.137
Qiang Yang,Jing Guo,Yuanfa Liu,Fucheng Guan,Fanchen Sun,Xueyong Gong
In order to compound gelatin (GEL) with poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3,4HB)) to improve the hydrophilicity and biocompatibility, a simple and convenient method for preparing P(3,4HB)/GEL composite nanofibrous (P/G-CNF) was proposed. Alkyl Polyglycoside (APG) was used as an emulsifier to prepare a stable P(3,4HB)/GEL composite solution, and novel P/G-CNF was prepared by electrospinning. The intermolecular interaction, micromorphology and porosity, crystallization and mechanical properties , hydrophilicity and cytocompatibility of P/G-CNF were characterized. The results reveal that P(3,4HB), APG and GEL form many hydrogen bonds . When the m(P(3,4HB):GEL) is 10:2, the as-prepared nanofibrous exhibit the best micromorphology with a diameter and porosity of 604 nm and 68.33%. With the increasing GEL content, the crystallization and mechanical properties of P(3,4HB)/GEL composite nanofibrous scaffold (P/G-CNS) are further enhanced and decline after reaching the maximum. The hydrophilicity of P/G-CNS is significantly improved by introducing GEL. The cell proliferation rate of P/G-CNS is higher than 100%, of which the cytotoxicity grade is 0, indicating that it is non-cytotoxic. The P/G-CNS has excellent cytocompatibility and provides better cell attachment, growth and proliferation, showing promising application prospects for tissue regeneration and biomedical materials .
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JOURNAL OF NATURAL PRODUCTS
·
2021
·
IF 4.05
Yaling Hui,Ting Tang,Jing Wang,Huanhuan Zhao,Hong-Ying Yang,Junmin Xi,Baoxin Zhang,Jianguo Fang,Kun Gao,Yueting Wu
Nonsmall cell lung cancer (NSCLC) has been a fatal and refractory disease worldwide. Novel therapeutic developments based on fundamental investigations of anticancer mechanisms underlie substantial foundations to win the fight against cancer diseases. In this study, we isolated a natural product fusaricide (FCD) from an endophytic fungus of Lycium barbarum, identified as Epicoccum sp. For the first time, we discovered that FCD potently inhibited proliferation in a variety of human NSCLC cell lines, with relatively less toxicity to normal cells. Our study exhibited that FCD induced apoptosis, caused DNA damage and cell cycle arrest in G0/G1 phase, and activated caspase-3 as well as other apoptosis-related factors in human NSCLC NCI-H460 cells. FCD was proven to be an iron chelator that actively decreased levels of cellular labile iron pool in NCI-H460 cells in our study. FeCl3 supplement reversed FCD-induced apoptosis. The upregulation of transferrin receptor 1 (TfR1) and downregulation of ferritin heavy chain (FTH) expression were observed after FCD treatment. In summary, our study highlighted the potential anticancer effects of FCD against human NSCLCs and demonstrated that the FCD-mediated apoptosis depended on binding to intracellular iron.
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JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Ke Yuan,Wencai Huang,Tao Zhang,Li Lv,Yan Wang,Zongpeng Zou,Shengwei Tang
Ionic Liquids (ILs) of 1,3-bis-(3-sulfonic acid)propyl imidazolium bisulfate ([PS 2 Im]HSO 4 ) can be used as acid catalyst for its specific acidity. However, it is difficult to obtain high-purity ILs by the reported synthetic methods. The preparation of [PS 2 Im]HSO 4 with N-trimethylsilylimidazole (TSIm) as raw material was studied. The results show that 1-(3-sulfonic acid)propyl-3-H-imidazolium bisulfate ([PSImH]HSO 4 ) and 1,3-bis-H-imidazolium bisulfate ([ImH]HSO 4 ) are easily formed in the process of preparing [PS 2 Im]HSO 4 from TSIm. Some TSIm is encapsuled in the formed solid intermediate 1-(propyl-3-sulfonate)-3-trimethylsilylimidazolium ([TSIm]PS), it leads to the formation of [ImH]HSO 4 . The solid intermediate [TSIm]PS can not be completely depleted in the following reaction for the formation of 1-(3-trimethylsilyl sulfonate)propyl-3-(propyl-3-sulfonate) limidazolium ([PSTSIm]PS). The residual solid intermediate [TSIm]PS leads to the formation of [PSImH]HSO 4 . A novel process using sodium imidazolate (ImNa) as raw material was proposed. [PS 2 Im]HSO 4 was successfully prepared with a purity of 96.2 mol.% and a yield of 69 %. The synthesis mechanism of high purity [PS 2 Im]HSO 4 was revealed by experimental research and DFT computation. Intermediate 1-(3-sodium sulfonate)propyl imidazole (PSNaIm) is formed by the reaction of ImNa and 1,3-PS with a molar ratio of 1:1. PSNaIm can easily be dissolved in DMSO and react with 1,3-PS to obtain 1-(3-sodium sulfonate)propyl-3-(propyl-3-sulfonate) imidazolium ([PSNaIm]PS). After cation exchange and reaction with sulfuric acid, high purity [PS 2 Im]HSO 4 is easily obtained. The results provide a guidance for the synthesis of high-purity imidazole-based ILs containing dual-acidic functional groups.
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2023
·Vol. 120
·期 45
·e2309032120
·
IF 11.1
Kaiyuan Yu,Qianqian Li,Xuan Sun,Xianping Peng,Qiang Tang,Hongyu Chu,Lu Zhou,Bangmao Wang,Zhemin Zhou,Xueqin Deng,Jianming Yang,Junqiang Lv,Ran Liu,Chunhui Miao,Wei Zhao,Zhi Yao,Quan Wang
Tryptophan and its derivatives perform a variety of biological functions; however, the role and specific mechanism of many tryptophan derivatives in intestinal inflammation remain largely unclear. Here, we identified that an Escherichia coli strain (Ec-TMU) isolated from the feces of tinidazole-treated individuals, and indole-3-lactic acid (ILA) in its supernatant, decreased the susceptibility of mice to dextran sulfate sodium–induced colitis. Ec-TMU and ILA contribute to the relief of colitis by inhibiting the production of epithelial CCL2/7, thereby reducing the accumulation of inflammatory macrophages in vitro and in vivo. Mechanistically, ILA downregulates glycolysis, NF-κB, and HIF signaling pathways via the aryl hydrocarbon receptor, resulting in decreased CCL2/7 production in epithelial cells. Clinical evidence suggests that the fecal ILA level is negatively correlated with the progression indicator of inflammatory bowel diseases. These results demonstrate that ILA has the potential to regulate intestinal homeostasis by modulating epithelium–macrophage interactions.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Chixuan Yao,Yaozhong Hu,Qisijing Liu,Jing-Min Liu,Xuemeng Ji,Huan Lv,Shuo Wang
To improve the performance of peanut allergen Ara h 3 detection, depending on boron and nitrogen carbon dots (B/ N -CDs), a nanobody (Nb) mediated dual-mode immunoassay was established, which combines the dominance of colorimetry with ratiometric fluorescence techniques. With the catalysis of Horseradish peroxidase (HRP), the oxidization of o-phenylenediamine (o-PD) in the presence of H 2 O 2 , leading to the production of yellow 2,3-diaminophenolazine (DAP) with an absorption peak at 431 nm. Owing to inner filter effect (IFE), DAP quenched the fluorescence of B/ N -CDs at 426 nm, and it emerged a new emission peak at 549 nm. The fluorescence intensity ratio and absorption intensity can be utilized for quantitative analysis of Ara h 3 concentration. Under optimal conditions, the detection limits were 6.61 and 9.79 ng·mL −1 , respectively. The dual-mode immunoassay was assessed containing specificity, stability, reproducibility, and practicability. This method paved the way for sensitive detection of Ara h 3 without background Interference.
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Journal of Analytical Science and Technology
·
2023
·Vol. 14
·期 1
·1-8
·
IF 2.4
Xu Ting,Qingru Zeng,Fu Qing,Wang Zhaojie,Liu Xin,Xiao Shensheng,Jiang Xiaoming,Lu Yuepeng,Gong Zhiyong,Wu Yongning,Fang Min
Using amino silica modified with fluorescein isothiocyanate (FITC), a quick fluorescence analysis technique is used for the detection of 3-monochloropropane-1,2-diol (3-MCPD). At 100 °C and pH 8.5, FITC-modified amino silica exhibits the lowest fluorescence intensity in the presence of 3-MCPD. This can predominantly be explained mostly explained by 3-MCPD’s capacity to occupy the amino group that FITC normally binds to. The fluorescence intensity of FITC-modified amino silica was greatly quenched by 3-MCPD’s reaction with the amino group under alkaline conditions, and the fluorescence intensity is different at different reaction times, reaction pH, and reaction temperature. The effects of various 3-MCPD concentrations on the optical characteristics of FITC-modified amino silica were also investigated. Fluorescence analysis is used to obtain a linear range from 0.025 to 1.0 mg/L for 3-MCPD detection under optimal experimental conditions, with a detection limit of 0.025 mg/L and a correlation coefficient of 0.9915. The quantity of 3-MCPD in soy sauce was measured under ideal conditions. Using the optimized conditions, the contents of 3-MCPD in soy sauce were determined. These results suggest that this method is sensitive to 3-MCPD and may have a substantial application in the rapid detection of food contaminants particularly, where the quality and safety of food products are of paramount concern.
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BRAIN RESEARCH
·
2023
·
IF 2.9
Yong-Juan Gu,He-Ya Qian,Fang Zhou,Ling Zhang,Long Chen,Yu Song,Ya-Nan Chen,Hai-Long Zhang
Background Bone cancer pain (BCP) remains a clinical challenge due to the limited and side effects of therapeutic methods. Folic acid has been known as an FDA approved dietary supplement and proved to have an analgesic effect in neuropathic pain. Here we investigate the role and mechanism of folic acid in bone cancer pain of a rat model. Methods Walker 256 tumor cells were inoculated into the left tibia of rats to induce bone cancer pain model. Pain reflex were assessed by paw withdrawal threshold (PWT) response to Von Frey filaments and paw withdrawal latency (PWL) response to thermal stimulation. Folic acid was injected intraperitoneally to evaluate its analgesic effect in rats with bone cancer pain. Western blotting and qPCR were used to determine P2X2/3 receptor protein and mRNA levels in ipsilateral L4-6 dorsal root ganglion (DRG) and spinal dorsal horn (SDH). Results The PWT and PWL of rats with bone cancer pain were obviously decreased compared to the naïve and sham rats. Interestingly, continuous folic acid treatment significantly increased the PWT and PWL of rats with bone cancer pain. P2X2 and P2X3 receptors were clearly upregulated at both mRNA and protein expression in L4-6 DRG and SDH of rats with bone cancer pain. P2X2 and P2X3 receptors were mainly localized with CGRP (calcitonin gene-related peptide) or IB4 (isolectin B4) positive neurons in L4-6 DRG of rats with bone cancer pain. Notably, continuous folic acid treatment significantly reduced the expression of P2X2 and P2X3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Finally, intrathecal injection of A317491 (a selective antagonist of P2X2/3 receptors) markedly elevated the PWT and PWL of rats with bone cancer pain. Conclusion These results suggest that folic acid has an effective antinociceptive effect on bone cancer pain, which is mediated by downregulating P2X2/3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Folic acid may be a novel therapeutic strategy in cancer patients for pain relief.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Peizhi Jia,Daxin Chen,Ying Zhu,Meiling Wang,Jianwei Zeng,Ling Zhang,Qiaoyan Cai,Dawei Lian,Chunyu Zhao,Yaoyao Xu,Jianfeng Chu,Shan Lin,Jun Peng,Wei Lin
Ethnopharmacological relevance Liensinine(Lien, C 37 H 42 N 2 O 6 ) is an alkaloid compound from plumula nelumbinis that demonstrates an antihypertensive effect. The protective effects of Lien on target organs during hypertension are still unclear. Aim of the study : This study aimed to understand the mechanism of Lien during the treatment of hypertension, with emphasis on vascular protection. Materials and methods Lien was extracted and isolated from plumula nelumbinis for further study. In vivo model of Ang II-induced hypertension, non-invasive sphygmomanometer was used to detect the blood pressure in and out of the context of Lien intervention. Ultrasound was used to detect the abdominal aorta pulse wave and media thickness of hypertensive mice, and RNA sequencing was used to detect the differential genes and pathways of blood vessels. The intersection of Lien and MAPK protein molecules was detected by molecular interconnecting technique. The pathological conditions of abdominal aorta vessels of mice were observed by HE staining. The expression of PCNA, α-SMA, Collagen Type Ⅰ and Collagen Type Ⅲ proteins were detected by IHC. The collagen expression in the abdominal aorta was detected by Sirius red staining. The MAPK/TGF-β1/Smad2/3 signaling and the protein expression of PCNA and α-SMA was detected by Western blot. In vitro , MAPK/TGF-β1/Smad2/3 signaling and the protein expression of PCNA and α-SMA were detected by Western blot, and the expression of α-SMA was detected by immunofluorescence; ELISA was used to detect the effect of ERK/MAPK inhibitor PD98059 on Ang Ⅱ-induced TGF-β1secrete; and the detection TGF-β1and α-SMA protein expression by Western blot; Western blot was used to detect the effect of ERK/MAPK stimulant12-O-tetradecanoyl phorbol-13-acetate (TPA) on the protein expression of TGF-β1 and α-SMA. Results Lien displayed an antihypertensive effect on Ang Ⅱ-induced hypertension, reducing the pulse wave conduction velocity of the abdominal aorta and the thickness of the abdominal aorta vessel wall, ultimately improving the pathological state of blood vessels. RNA sequencing further indicated that the differential pathways expressed in the abdominal aorta of hypertensive mice were enriched in proliferation-related markers compared with the Control group. The profile of differentially expressed pathways was ultimately reversed by Lien. Particularly, MAPK protein demonstrated good binding with the Lien molecule. In vivo, Lien inhibited Ang Ⅱ-induced abdominal aorta wall thickening, reduced collagen deposition in the ventral aortic vessel, and prevented the occurrence of vascular remodeling by inhibiting MAPK/TGF-β1/Smad2/3 signaling activation. In addition, Lien inhibited the activation of Ang II-induced MAPK and TGF-β1/Smad2/3 signaling, attenuating the expression of PCNA and inhibiting the reduction of α-SMA, collectively playing a role in the inhibition of Ang Ⅱ-induced hypertensive vascular remodeling. PD98059 alone could inhibit Ang Ⅱ-induced elevation of TGF-β1 and the decrease of α-SMA expression. Further, PD98059 combined with Lien had no discrepancy with the inhibitors alone. Simultaneously TPA alone could significantly increase the expression of TGF-β1 and decrease the expression of α-SMA. Further, Lien could inhibit the effect of TPA. Conclusion This study helped clarify the protective mechanism of Lien during hypertension, elucidating its role as an inhibitor of vascular remodeling and providing an experimental basis for the research and development of novel antihypertensive therapies.
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JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
·
2019
·
IF 4.027
Jie Yang,Pingping Zhang,Yuheng Hu,Teng Liu,Jie Sun,Xiaojing Wang
Alzheimer's disease, a neurodegenerative illness, has the extremely complex pathogenesis. Accumulating evidence indicates there is a close relationship between several enzymes and Alzheimer's disease. Various substituted 3-arylcoumarin derivatives were synthesised, and their in vitro activity, including cholinesterase inhibitory activity, monoamine oxidase inhibitory activity, and antioxidant activity were investigated. Most of the compounds exhibited high activity; therefore 3-arylcoumarin compounds have the potential as drug candidates for the treatment of Alzheimer's disease.
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ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yuze Xie,Mingwei Wang,Xi Chen,Shufen Wang,Dandan Han,Yehong Han,Hongyuan Yan
A facile and efficient method for the determination of triazine herbicides in tomato samples was developed by employing 3-aminophenol-glyoxylic acid resin microspheres as a solid-phase extraction adsorbent followed by high performance liquid chromatography analysis. These resin microspheres were synthesized by a simple green precipitation polymerization method, and a range of functional groups (hydroxyl, amino, carboxylic group) were introduced through the 3-aminophenol and glyoxylic acid components. The as-prepared resin microspheres were characterized by scanning electron microscopy, Fourier transform infrared spectrometry , and thermal gravimetric analyzer. The resin microspheres exhibited a good adsorption rate, large adsorption amount, and short adsorption equilibrium time (almost in ∼5 min). Under the optimal extraction and determination conditions, a good linearity was obtained in the range of 0.025–7.5 μg g −1 (r 2 ≥ 0.9997) for atraton , ametryn , and prometryn . The limits of detection of atraton, ametryn, and prometryn were 0.57, 0.75, and 1.06 μg kg −1 , respectively. The intra-day and inter-day precisions expressed as relative standard deviations were in the ranges of 1.8–3.2% and 1.7–4.1%, respectively. In addition, the recoveries at three spiked levels ranged from 85.1 to 97.7% with the relative standard deviation ≤ 5.4% (n = 3). This novel method is simple and accurate and has proved to be a reliable alternative method for the determination of triazine herbicides in tomato samples.
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POLYMER
·
2019
·
IF 3.771
Fuzhou Wang,Guoyong Xu,Qingshan Li,Ryo Tanaka,Yuushou Nakayama,Takeshi Shiono
The first example of a 3-alkene polymerization has been successfully achieved with 3-heptene in the presence of phenyl-substituted α- diimine nickel precatalysts activated by dMMAO, and compared with the corresponding linear alkenes, i.e., 1-pentene, 2-hexene and 4-octene polymerizations. The microstructures of the produced poly(3-heptene), which consists of ethyl , propyl and butyl branches indicated the 3,4- and 4,3-insertion, followed by chain-walking, with incorporation of a small fraction of long methylene sequences of 1,7-enchainment via monomer-isomerization. Late-transition metal catalyzed polymerization reactions of internal alkenes to synthesize novel polyolefin materials are highly important and also highly challenging. Upon activation with dried modified methylaluminoxane (dMMAO), the first example of a 3-alkene polymerization has been successfully achieved for 3-heptene catalyzed by phenyl-substituted α- diimine nickel complexes in comparison, and compared with the polymerizations of the corresponding linear alkenes, i.e., 1-pentene, 2-hexene and 4-octene. The results indicate that the polymerization of 3-heptene was achieved via chain-walking to generate branched amorphous polymers with glass transition temperature of ca. −68 °C. The microstructures of the produced poly(3-heptene) indicated the 3,4- and 4,3-insertion followed by chain-walking, the latter being predominant. The NMR analyses of the polymers showed that the obtained poly(3-heptene) possessed ethyl, propyl and butyl branches and a small amount of the long methylene sequence of 1,7-enchainment via monomer-isomerization. The branches structures were significantly dependent on the polymerization temperature, and the content of propyl branches derived from 3,4-insertion decreased with an increase in the polymerization temperature.
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