ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Ni Hui,Xiaotian Sun,Shuyan Niu,Xiliang Luo
Biofouling arising from nonspecific adsorption is a substantial outstanding challenge in diagnostics and disease monitoring, and antifouling sensing interfaces capable of reducing the nonspecific adsorption of proteins from biological complex samples are highly desirable. We present herein the preparation of novel composite nanofibers through the grafting of polyethylene glycol (PEG) polymer onto polyaniline (PANI) nanofibers and their application in the development of antifouling electrochemical biosensors. The PEGylated PANI (PANI/PEG) nanofibers possessed large surface area and remained conductive and at the same time demonstrated excellent antifouling performances in single protein solutions as well as complex human serum samples. Sensitive and low fouling electrochemical biosensors for the breast cancer susceptibility gene (BRCA1) can be easily fabricated through the attachment of DNA probes to the PANI/PEG nanofibers. The biosensor showed a very high sensitivity to target BRCA1 with a linear range from 0.01 pM to 1 nM and was also efficient enough to detect DNA mismatches with satisfactory selectivity. Moreover, the DNA biosensor based on the PEGylated PANI nanofibers supported the quantification of BRCA1 in complex human serum, indicating great potential of this novel biomaterial for application in biosensors and bioelectronics.
研究领域:
材料科学
·
生物传感器
·
生物医学工程
·
电化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 8
·1104-1115
·
IF 0
Bhatti Huma,Jawed Rohil,Liu Hang,Ali Irshad,Zhu Libo,Liu Quanjun
Biological nanopores have been proved as impressive nano-devices capable of holding the detection sensitivity from a nanoparticle to a single macromolecule. Protein pores are efficiently reproducible with assured channel geometry and their propensity to be engineered easily make them
convenient in nanopore sensing techniques. Among various porin-proteins, MspA porin of Mycobacterium smegmatis is quite ideal for nanopore DNA sequencing. However, the traversing speed of singlestranded DNA (ssDNA) through both the mutants of MspA (M1MspA and M2MspA) is too fast to
be analyzed. Therefore, we utilized a new mutant of MspA, termed as M3MspA which was previously purified in our laboratory. We, for the first time, demonstrated that translocation of ssDNA is achieved at a minimum voltage-threshold of 40 mV at pH 8.0. This new mutant of MspA exhibits a crystal-clear
discrimination between two homopolymers, poly dA50 and poly dC50 with duration time up to few milliseconds and well-resolved signature current-amplitudes were obtained for both sides (3′ or 5′) of the polymer entered the pore, without any modification to ssDNA.
Significantly, M3MspA determines a precise length of a hetero-homopolymer, poly(dA50dC50) by producing a dwell time congruent to the sum of the time spent by their component-homopolymers, separately. On account of their specificity and sensitivity towards translocating
polynucleotides, we suggest that this new mutant of MspA would be a sophisticated bio-nanopore in further enlightening the translocation dynamics caused by diverse conformations of a same biomolecule. On top of that, it would also be helpful in nanopore DNA sequencing.
研究领域:
生物物理学
·
纳米技术
·
分子生物学
·
基因组学
✓ 已验证
DOI
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Zhenzhen Xu,Zhigang Wang,Dingmeng Hu,Huiqin Chen,Yufei Yan,Yuan Li,Mengjing Tu,Qiaofeng Shen,Xuanyi Liu,Rui Li,Cheng Lu,Fei Xue,Congying Xie,Gen Yang
5-Hydroxymethylcytosine in genomic DNA (5hmC-DNA), a predominant epigenetic mark, plays key roles in a broad range of bioprocesses such as cancer progression. However, efficient detection of 5hmC-DNA, especially from highly complex biological specimens, remains a harsh challenge due to its low abundance as well as the serious disturbance of non-targets. Leveraging MXene as the signal booster, an electrochemical biosensing technique is presented here for ultrasensitive detection of 5hmC-DNA from complex biological samples. The developed technique integrates enzyme-catalyzed transform of the hydroxyl group of 5hmC, sequence-specific recognition of the 5hmC-DNA targets, and facile electrochemical reaction. Remarkably, nanosized Ti 3 C 2 T x MXene is introduced as a highly conductive agent, managing to greatly boost the signals. Exhibiting outstanding consistency and robustness, the technique not only achieves excellent linear responses in a broad range of 5hmC-DNA (1.0 × 10 −13 –1.0 × 10 −9 m ) with the lowest detection limit of 59 fM but also possesses excellent selectivity against 5hmC analogs of including cytosine (C)- and 5-methylcytosine (5mC). More importantly, a proof-of-concept validation demonstrates the extraordinary ability of this technique for directly detecting 5hmC-DNA from complex backgrounds including cellular genomic extracts and serum of irradiated or non-irradiated mice, suggesting its highly promising potential for diverse applications such as early cancer screening.
研究领域:
分子生物学
·
分析化学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
Pharmacognosy Magazine
·
2025
·
IF 0.9
Qing Liu,Ying Lan,Zhe Bai
BackgroundHyperoside is a potential liver cell and mitochondrial protector, but there is no evidence to suggest that hyperoside can effectively treat doxorubicin (DOX) related liver toxicity.PurposeThis study aims to determine the role of hyperoside in DOX related liver toxicity and liver cell damage in vivo and in vitro.MethodsA mouse model of liver toxicity was induced by intraperitoneal injection of DOX, and hyperoside was administered orally for treatment. During this period, the weight and food intake of the mice were recorded. The morphology of mouse liver tissue was observed by hematoxylin and eosin staining. Oxidative stress and inflammatory status in mice were observed through serum inflammatory factors and liver oxidative stress markers. Mitochondrial damage is determined by the degree of mitochondrial DNA damage. Western blotting and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of pyroptosis and genes in the liver and liver cells.ResultsHyperoside can effectively treat DOX-induced oxidative stress and inflammatory status, liver function damage, and hepatocyte necrosis in mice. Further research suggests that the beneficial effect of hyperoside is achieved by inhibiting mitochondrial DNA damage in liver cells.ConclusionThis study indicates that hyperoside improves DOX-induced liver toxicity by inhibiting mitochondrial DNA damage.
研究领域:
药理学
·
肝脏病学
·
分子生物学
✓ 已验证
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Qiaohui Chen,Yongqi Yang,Xiaohong Ying,Changkun Huang,Jianlin Chen,Jue Wang,Ziyu Wu,Wan Zeng,Chenxi Miao,Xiaojing Shi,Yayun Nan,Qiong Huang,Kelong Ai
Acute kidney injury (AKI) manifests a hallmark pathological feature of extensive and severe DNA damage in renal tubules, primarily induced by the excessive of toxic reactive oxygen species (ROS) from the mitochondrial electron transport chain. The kidney's complex intricate physiological architecture and the heterogeneous intracellular environment pose significant challenges for effective sequential and high-resolution drug delivery—an urgent issue that remains unresolved. To address this, a hierarchical-targeting antioxidant nanodrug has been developed with a folic acid moiety (HAND) designed for high-resolution drug delivery in AKI treatment. For the first time, HAND enables sequential targeting from the kidney to the most severely damaged proximal tubular epithelial cells (PTECs), ultimately concentrating in the DNA-rich mitochondria and nucleus. As a result, HAND effectively scavenges ROS in situ, protecting both mitochondria and nuclei along with their vital genetic material. This action restores mitochondrial function, mitigates DNA oxidation and fragmentation, reduces apoptosis, and inhibits cGAS/STING-mediated sterile inflammation. Consequently, HAND demonstrates remarkable efficacy in safeguarding injured kidneys during AKI. Overall, this work pioneers a hierarchical, high-resolution antioxidant strategy, providing innovative guidance for the development of AKI therapies.
研究领域:
药理学
·
分子生物学
·
肾脏病学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Peng Huang,Jingwen Wang,Long Jiao,Dandan Gu,Shusen Jiang,Mingpo Li,Wenlong lv,Hong Chen,Hao Pei
The emergence of DNA origami greatly accelerated the development of DNA nanotechnology. A thorough understanding of origami thermodynamics is very important for both fundamental studies and practical applications. These thermodynamic transitions usually take place in several seconds or even less, and are very difficult to monitor by conventional methods. Numerous tests are required to characterize the origami molecule's behaviors at different temperatures, which is very labor-intensive and time-consuming. In this paper, an axially distributed temperature gradient along a capillary was formed in a spatially varying temperature field. In such a temperature gradient, the origami molecule's thermodynamic processes occur and remain stable at every position along the capillary's microchannel . It looks like the time of the thermodynamic process is frozen along the microchannel. With this method, the origami molecule's thermodynamic characteristics at different temperatures can be obtained in a single experiment, and rapid processes can be monitored with ease by conventional methods for an adequate time period at low cost. In order to show its potential abilities, this method has been demonstrated in applications which the origami's assembly, denaturation and strand displacement are carry out in a flowing or stationary solution.
研究领域:
生物物理学
·
纳米技术
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2017
·Vol. 34
·期 1
·28-32
·
IF 1.024
Zhang Hong,Zou Li,Li Ruimin,Zhao Mingqin,Ling Liansheng
Simian virus 40(SV40) is a polyomavirus and can induce a series of different tumors. The recognition of SV40 genome is crucial to tumor diagnosis and gene therapy. Herein, a sensitive and selective colorimetric method for sequence-specific recognition of homopyrimidine·homopurine duplex DNA(dsDNA) of SV40(4424—4440, gp6) was established with a hairpin probe based upon the formation of triplex DNA. Hairpin probe 5′-CCC TAC CCA TTT TTT CTT CTC TTT CCT GGG TAG GGC GGG TTG GG-3′(HP) containing G-rich sequence and 17-bp trip-lex-forming sequence was used as the signal probe, which was stem-loop structure alone and exhibited low catalytic activity. Upon its binding to the target duplex of SV40, hairpin probe transferred from stem-loop structure to parallel triplex DNA, accompanied by the recovery of catalytic activity of DNAzyme and a sharp increase of absorbance. Under optimum conditions, the absorbance was increased proportionally to the concentration of dsDNA over the range from 500 pmol/L to 40.0 nmol/L with a detection limit of 433 pmol/L. Moreover, satisfied results were obtained when the assay was used to recognize the mismatched sequences.
研究领域:
分子生物学
·
肿瘤诊断
·
基因治疗
✓ 已验证
产品关联
DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2013
·Vol. 214
·期 20
·2266-2275
·
IF 2.386
Jixian Liu,Zhen Liu,Xiong Luo,Xidan Zong,Jingquan Liu
The preparation of biodegradable and positively charged graphene–polymer nanocomposite for DNA uptake and release is presented. A pyrene-terminated poly(2-(dimethylamino) ethyl acrylate) is firstly synthesized via reversible addition–fragmentation chain transfer (RAFT) polymerization, followed by the attachment onto the graphene surface via the π–π stacking interaction. The positively charged polymer brushes benefit the uptake of the negatively charged DNA fragment via static interaction and the built-in disulfide linkage between the polymer chain and graphene surface allows the biodegradable release of DNA in the presence of reducing agents. X-ray photoelectron spectroscopy manifests the binding of DNA molecules to the polymer chains and agarose gel electrophoresis confirms the release of DNA.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2015
·
IF 2.96
Jie-Hua Shi,Ting-Ting Liu,Min Jiang,Jun Chen,Qi Wang
The binding interaction of gefitinib with calf thymus DNA (ct-DNA) under the simulated physiological pH condition was studied employing UV absorption, fluorescence, circular dichroism (CD), viscosity measurement and molecular docking methods. The experimental results revealed that gefitinib preferred to bind to the minor groove of ct-DNA with the binding constant ( K b ) of 1.29 × 10 4 L mol −1 at 298 K. Base on the signs and magnitudes of the enthalpy change (Δ H 0 = −60.4 kJ mol −1 ) and entropy change (Δ S 0 = −124.7 J mol −1 K −1 ) in the binding process and the results of molecular docking, it can be concluded that the main interaction forces between gefitinib and ct-DNA in the binding process were van der Waals force and hydrogen bonding interaction. The results of CD experiments revealed that gefitinib did not disturb native B-conformation of ct-DNA. And, the significant change in the conformation of gefitinib in gefitinib–ct-DNA complex was observed from the molecular docking results and the change was close relation with the structure of B-DNA fragments, indicating that the flexibility of gefitinib molecule also plays an important role in the formation of the stable gefitinib–ct-DNA complex.
研究领域:
生物化学
·
药物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2018
·
IF 3.789
Fengyun Li,Qi Gao,Mingjie Yang,Weiwei Guo
The regulation of the activities of catalytic DNA is of great importance in many applications, especially in biosensing, controllable drug carriers, and gene therapy. In this work, the surfaces of gold nanoparticles (AuNPs) are simultaneously modified with a thermoresponsive polymer, poly(N-isopropylacrylamide) (pNIPAM), and catalytic DNA to form thermosensitive catalytic DNA/pNIPAM/AuNP systems. The thermosensitive pNIPAM on the surfaces of AuNPs enables the temperature-controlled catalytic activities of the system in a narrow temperature range. The catalytic DNA/pNIPAM/AuNP system exhibits almost no catalytic activity at temperatures below the lower critical solution temperature (LCST) of pNIPAM and become highly catalytic when the temperature is higher than the LCST. Two kinds of catalytic DNA, the entropy-driven DNA catalytic network and the Mg2+-dependent DNAzyme, were chosen as model catalytic systems, and the results showed that the regulation of catalytic activities for both systems was achieved efficiently. These systems may have important potentials in future biosensing and biomedical applications.
研究领域:
生物化学
·
纳米医学
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2012
·
IF 2.102
Shujuan Xu,Yong Shao,Fei Wu,Guiying Liu,Lingling Liu,Jian Peng,Yanwei Sun
Many efforts have been made for sequence-specific recognition of DNA by small molecules. In this study, abasic site (AP site) in a DNA duplex was found to be targeted by myricetin (Myr), one of the natural 3-hydroxyflavonols. Steady-state and transient-state fluorescence, FRET , and DNA melting experiments confirmed that the AP site binding of Myr favors an emission from its tautomer that is derived from excited-state intramolecular proton transfer (ESIPT) reaction between the 3-OH and 4-carbonyl moieties. The selective recognition of Myr is less dependent on the flanking bases of the AP site, although the ESIPT emission is more lighting up for the DNAs with cytosine and thymine opposite the AP site. Because Myr alone in aqueous solution is non-fluorescent, this selective lighting-up emission is advantageous for developing a practical sensor to target the DNA AP site with a weak fluorescence background. This selective recognition of the AP site by the lighting-up fluorescence response would find wide applications including efficiently evaluating DNA damage/repair and screening antitumor/antioxidation drugs.
研究领域:
化学
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 14
·9595-9602
·
IF 3.9
Jia Xu,Yuan Yang,Xiaonan Cai,Han Xiao
In this study, hexafluoroisopropanol (HFIP)-based deep eutectic solvents (DESs) were developed and used for DNA extraction from human whole blood samples for the first time. HFIP-based DESs were prepared using HFIP and choline chloride (ChCl)/tetrabutylammonium chloride/cetyltrimethylammonium bromide as the hydrogen bond donor and acceptor, respectively. The two-phase system formation was promoted with different inorganic salts as the phase-forming component. According to the strong phase separation capability and high DNA extraction efficiency, DESs consisting of HFIP/ChCl-(NH4)2SO4, HFIP/ChCl-Na2SO4 and HFIP/ChCl-MgSO4 were then selected for application in DNA extraction. The factors that could have impacted the DNA extraction process, including molar ratio of DES, DES addition, salt species and addition, and sample pH, were systematically investigated via single-factor experimental analysis. Furthermore, we selectively examined bovine serum albumin and RNA to assess the specificity of the HFIP-based DESs for DNA extraction. Conclusively, 93.9%, 96.7% and 99.8% DNA could be extracted using the HFIP/ChCl-(NH4)2SO4, HFIP/ChCl-Na2SO4, and HFIP/ChCl-MgSO4 systems, respectively. Moreover, the developed systems were successfully used to extract DNA from human whole blood with satisfactory results. The DNA secondary structure was stable after DES extraction with the electrostatic interaction between DES and DNA as the main force driving DNA adsorption by DES.
研究领域:
化学
·
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）