JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Lili Song,Jiayi Wang,Jiaxuan Nie,Yue Zhang,Rui Han,Huimin Liu,Ningning Ma,Zhen Yang,Yubo Li
Ethnopharmacological relevance Tripterygium wilfordii Hook. f (TwHF) has been used as a traditional Chinese medicine for the treatment of rheumatoid arthritis and nephritis for hundreds of years. Aim of the study Although the efficacy of TwHF in the treatment of RA is definite, its serious side effects and toxicity have also received close attention from domestic and international researchers, so the clinical application of TwHF has been controversial. Most of the current TwHF toxicity studies have been conducted with animals in normal body states, but ignore the effects in pathological states. In this study, we aimed to find out the material basis and metabolic mechanism of the “toxicity/effectiveness” of TwHF on rat kidneys in different body states by using two-way orthogonal partial least squares (O2PLS) method. Materials and methods In the present study, TwHF was extracted by reflux extraction method using ethanol as the extraction solvent. Firstly, the effects of TwHF on rat kidneys in different body states were first evaluated by detecting creatinine and urea nitrogen levels and morphological changes in kidney pathology identified the components of TwHF in rats in different body states using UPLC-Q-TOF/MS technique. Serum and urine metabolomics were used to search for biomarkers and metabolic pathways by which TwHF exerts renal injury and protection, and finally, O2PLS correlation analysis was used to correlate the components with renal protective and injury biomarkers. Results TwHF was found to have a protective effect on the kidney of RA rats and an injurious effect on the kidney of normal rats at a dose of 11.25 g/kg/d. The UPLC-Q-TOF/MS technique was used to identify 34 components in TwHF extracts; 23 components and 57 metabolites were identified in the administered rats. O2PLS screened three substances as both toxic and pharmacodynamic components of TwHF, namely 3,5-dimethoxyphenyl-2-propenl-ol, kaurane-16,19,20-triol, and demethylzeylasteral + O, and found that these three components may exert nephrotoxic effects via the nicotinic acid and nicotinamide metabolic pathways and nephroprotective effects via the tryptophan metabolic pathway. Conclusion In this study, O2PLS analysis was used for the first time to combine biomarkers and components in vivo and found the material basis and metabolic mechanism of nephrotoxicity and efficacy of TwHF, which provided key clues for further study on the biological mechanism of toxicity and efficacy of TwHF.
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Oncology Letters
·
2022
·Vol. 24
·期 5
·1-12
·
IF 3.111
Haoran Jiang,Xianxin Wen,Xue Zhang,Bingyu Zhang
Concanavalin A (Con A), the first and most typical representative of the legume lectin family, has a potent anti‑liver cancer effect by inducing cell apoptosis and autophagy. However, its function in human liver cancer cell migration remains unclear. The present study investigated the effect of Con A on the viability and migration of human liver cancer cells with different metastatic abilities. It was found that Con A could reduce the viability of human liver cancer cells (HCCLM3, MHCC97L and HepG2) and human hepatocytes (MIHA). In addition, Con A could inhibit human liver cancer cell migration specifically without affecting hepatocytes. Sugar inhibition assay showed that glucose‑related sugar binding sites played an important role in the inhibition of Con A on human liver cancer cell migration. In addition, Con A could affect HCCLM3 migration by activating ERK1/2, JNK1/2/3 and p38 signaling in the MAPK pathway. Moreover, Con A could regulate fibrous actin (F‑actin) redistribution and assembly via the MAPK signaling pathway. However, Con A had no significant effect on the activation of matrix metalloproteinase (MMP)‑2 and MMP‑9 in HCCLM3 cells. In conclusion, Con A may bind to glucose‑related receptor protein, activating ERK1/2, JNK1/2/3 and p38 signaling in the MAPK pathway, further contracting cells and reducing the content of F‑actin of single cells to inhibit HCCLM3 cell migration. These results would deepen the links between human liver cancer cell migration and related glycoproteins or signaling molecules, and may provide a new perspective for Con A as a potential anticancer agent.
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DESALINATION
·
2022
·
IF 11.211
Wenxin Zhang,Yaqi Zhang,Yuze Wang,Shiwei Tian,Na Han,Wei Li,Wei Wang,Hongwei Liu,Xuhuan Yan,Xingxiang Zhang
Membrane fouling remains a significant restriction for the long-time operation. In this paper, fluffy-like amphiphilic graphene oxide (f-GO) was prepared by simple grafting silane coupling agent (KH-570) onto the graphene oxide matrix, and thus featured with good dispersion behavior in organic solution. The f-GO was further incorporated into the PAN-based (poly(acrylonitrile-methyl acrylate), P(AN-MA)) copolymer during the phase inversion process. Compared with the P(AN-MA) membrane, the resistance of the f-GO/P(AN-MA) composite membrane reduced from 4.49 × 10 −12 m −1 to 1.78 × 10 −12 m −1 . The pure water flux increased from 200 to 326 L/m 2 h, while the water contact angle declined from 92.8° to 68.3°. It is worthy to note that the fouling mode changed from irreversible fouling to reversible fouling , with the total fouling rate showing a half decay. The fouling recovery rate reached 98.16% and the BSA rejection rate increased from 70.59% to 99.33% as 0.5 wt% f-GO was incorporated. The f-GO is easily scaled up and can be widely applied in other polymer matrices , showing potential application prospects in the membrane separation and purification area. The f-GO/P(AN-MA) composite membranes with excellent water flux, BSA rejection and FRR, are candidates for further employment in wastewater purification applications.
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COMBUSTION AND FLAME
·
2023
·
IF 4.4
Yuxiang Li,Kaili Zhang
Metastable intermolecular composites (MICs) comprising solid fuels and oxidizers at nano-scale exhibit high energy densities and fast reaction speeds. As most metal and metalloid fuels are passivated by the natural oxide layer on the surface which acts as a reaction barrier, fluorine-containing oxidizers are of particular interest to promote the reactivity through interfacial fluoridation. In this work, cobalt hydroxy fluoride (Co(OH)F) was introduced as a functional fluorine-containing oxidizer, and core-shell structured Co(OH)F@Al nanoarray was fabricated onto a glass substrate . The thermal behavior and reaction mechanism of pure Co(OH)F and Co(OH)F@Al composites were thoroughly investigated, and Co(OH)F@Al was compared with CoO@Al and Co 3 O 4 @Al with identical morphology. In the Co(OH)F@Al composite, a pre-ignition reaction was observed which was attributed to the etching of Al 2 O 3 shell by HF and the fluoridation of Al core by HF and CoF 2 . With the assistance of pre-ignition reaction, Co(OH)F@Al can complete main exothermal reaction before Al melting in a solid-state mechanism, and its peak temperature is around 200 ℃ lower than those of MICs employing CoO and Co 3 O 4 as oxidizers.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Yanwei Zhu,Lang Gan,Jianqiao Shi,Gen Huang,Hongmei Gao,Li Tao,Shuangyin Wang
The structural design and atomic adjustment of the catalyst are the main factors that regulate the intrinsic electrocatalytic activity. Herein, we report a novel and facile strategy of synthesizing three-dimensional porous carbon network by polymer-assisted molding strategy. The porous carbon framework with Co-CoF 2 and carbon nanotubes (PCF@CCFCNT) was directly obtained by the calcination, in which Co-CoF 2 heterojunctions uniformly dispersed in the interconnected holes. Benefiting from the special hierarchical porous morphology, the dual-function catalytic activity of Co-CoF 2 heterojunctions and existence of Co-N x species, the as-obtained PCF@CCFCNT composites exhibit excellent performance in the oxygen evolution reaction (η = 300 mV@10 mA cm −2 ), as well as outstanding oxygen reduction reaction performance in alkaline medium (E 1/2 = 0.852 V, vs RHE). Moreover, as an air electrode in Zn-air batteries (ZABs), PCF@CCFCNT demonstrates a large peak power density of 184 mW cm −2 and superior long-term stability, which is much more stable than the one consisting of commercial Pt/C. This finding not only shows a simple composite design to achieve high-performance ZABs but also may stimulate the rapid development of 3D hierarchical porous electrocatalysts for advanced energy technologies.
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PLoS Pathogens
·
2021
·Vol. 17
·期 12
·e1010145
·
IF 6.823
Kaiyu Chen,Siyuan Zhang,Yina Shao,Ming Guo,Weiwei Zhang,Chenghua Li
Many members of the nucleotide-binding and oligomerization domain (NACHT)- and leucine-rich-repeat-containing protein (NLR) family play crucial roles in pathogen recognition and innate immune response regulation. In our previous work, a unique and Vibrio splendidus -inducible NLRC4 receptor comprising Ig and NACHT domains was identified from the sea cucumber Apostichopus japonicus , and this receptor lacked the CARD and LRR domains that are typical of common cytoplasmic NLRs. To better understand the functional role of AjNLRC4, we confirmed that AjNLRC4 was a bona fide membrane PRR with two transmembrane structures. AjNLRC4 was able to directly bind microbes and polysaccharides via its extracellular Ig domain and agglutinate a variety of microbes in a Ca 2+ -dependent manner. Knockdown of AjNLRC4 by RNA interference and blockade of AjNLRC4 by antibodies in coelomocytes both could significantly inhibit the phagocytic activity and elimination of V . splendidus . Conversely, overexpression of AjNLRC4 enhanced the phagocytic activity of V . splendidus , and this effect could be specifically blocked by treatment with the actin-mediated endocytosis inhibitor cytochalasin D but not other endocytosis inhibitors. Moreover, AjNLRC4-mediated phagocytic activity was dependent on the interaction between the intracellular domain of AjNLRC4 and the β-actin protein and further regulated the Arp2/3 complex to mediate the rearrangement of the cytoskeleton and the polymerization of F-actin. V . splendidus was found to be colocalized with lysosomes in coelomocytes, and the bacterial quantities were increased after injection of chloroquine, a lysosome inhibitor. Collectively, these results suggested that AjNLRC4 served as a novel membrane PRR in mediating coelomocyte phagocytosis and further clearing intracellular Vibrio through the AjNLRC4-β-actin-Arp2/3 complex-lysosome pathway.
Vibrio splendidus is ubiquitously present in marine environments and in or on many aquaculture species and is considered to be an important opportunistic pathogen that has caused serious economic losses to the aquaculture industry worldwide. Phagocytosis is the first step of pathogen clearance and is triggered by specific interactions between host pattern recognition receptors (PRRs) and pathogen-associated molecular patterns (PAMPs) from invasive bacteria. However, the mechanism that underlies receptor-mediated V . splendidus phagocytosis is poorly understood. In this study, an atypical AjNLRC4 receptor without LRR and CARD domains was found to serve as the membrane receptor for V . splendidus , not the common cytoplasmic NLRs. The Ig domain of AjNLRC4 is replaced with a conventional LRR domain to bind V . splendidus , and the intracellular domain of AjNLRC4 specifically interacts with β-actin to mediate V . splendidus endocytosis in an actin-dependent manner. Endocytic V . splendidus is ultimately degraded in phagolysosomes. Our findings will contribute to the development of novel strategies for treating V . splendidus infection by modulating the actin-dependent endocytosis pathway.
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TALANTA
·
2023
·
IF 6.1
Qiong He,Ying Chen,Yuyang Liu,Qiang Wang,Chiyang He,Shaorong Liu
Large–size spherical sorbents with particle size of 10–50 μm are widely applied in separation fields, however it is still a great challenge to synthesize such large–size spherical covalent organic framework (COF). In this work, a type of large–size porous 3D COF was size–controablly synthesized via a two–step strategy, in which a large–size porous 3D spherical polymer was prepared first through a Pickering emulsion polymerization using nano silica as the stabilizer, and subsequently it was converted into porous spherical 3D COF by a solvothermal method . The as–prepared porous spherical COF (COF–320 as a model) showed size–controllable uniform spherical morphology within 15–45 μm, large specific surface area, fine crystalline structure, and good chemical stability. When used as the sorbent for dispersive solid–phase extraction (d–SPE) of bisphenol F (BPF), the porous spherical COF–320 (15 μm) displayed high adsorption capacity ( Q max = 335.6 mg/g), high enrichment factor (80 folds), and good reusability (at least five cycles). By coupling the d–SPE method to HPLC, a new analytical approach was developed and successfully applied to the determination of trace BPF in two water samples, an orange juice and a standard sample with recoveries of 96.0–102.2 % (RSD = 1.1–1.5 %), 95.7–97.4 % (RSD = 1.4–4.4 %) and 98.7 % (RSD = 2.3 %), respectively. The limit of detection (S/N = 3) and limit of quantification (S/N = 10) were 0.1 and 0.3 ng/mL, respectively. The new synthesis strategy opens a viable way to prepare large–size porous spherical COFs, and the developed analytical method can be potentially applied to sensitively detect the trace BPF in water samples and beverages.
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CARBON
·
2023
·
IF 10.9
Guodong Shi,Jian Song,Xiaoxiao Tian,Tongtong Liu,Zhanjun Wu
Graphene oxide (GO) is an ideal reinforcement for improving mechanical properties of epoxy (EP) and reducing its coefficient of thermal expansion (CTE). However, the poor interfacial interaction between GO and polymer matrix seriously hinders the reinforcing effect of GO. To improve their interface, we first functionalized GO with flexible polyetheramine (PEA) and f-SiO 2 rigid nanoparticles, obtaining PEA-GO and SiO 2 -PEA-GO hybrids, respectively. Then the PEA-GO and SiO 2 -PEA-GO were incorporated into EP to obtain nanocomposites with flexible interphases and rigid-flexible interphases, respectively. The interfaces were tuned by two PEA molecules (D230 and D2000) with different chain lengths. The effects of the different interface structures on the mechanical properties and CTE of the GO/EP nanocomposites with a 0.25 wt% filler loading were investigated. It was found that the effects of SiO 2 -PEA-GO on strengthening and toughening EP matrix and on reducing its CTE were much better than those of PEA-GO, because the rigid-flexible interfaces provided higher bonding strength and better energy dissipation mechanisms than the flexible interfaces. Moreover, the D230-GO/EP nanocomposite containing shorter PEA (D230) molecules exhibited higher strength but lower toughness and CTE than the D2000-GO/EP nanocomposite. However, the SiO 2 -D2000-GO/EP nanocomposite showed greater strength, toughness and CTE than the SiO 2 -D230-GO/EP nanocomposite. This indicated that the longer PEA molecules in rigid-flexible interphases produced higher improvement in strength and toughness but smaller decreases in CTE. This work provides a promising strategy of constructing an adjustable flexible-rigid interfacial structure and opens an avenue for developing GO/EP nanocomposites with high mechanical properties and low CTE.
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CATALYSIS LETTERS
·
2021
·Vol. 151
·期 11
·3170-3188
·
IF 3.186
Zhang Qian,Zhou Yu-lian,Wang Bao-zong,Hong Jun-ming
The performance of electrocatalytic-oxidation process depends on the anode characteristics, so the preparation of active anode materials is an attractive method to enhance the efficiency of this process. However, the high catalytic properties of graphene and its derivatives have been well confirmed, an efficient method of controlling catalytic activity and stability remains to be established. In this research, the relationship between the precursor and catalytic performance was clarified by regulating the oxygen-containing groups in the precursor. Results indicated that the surface O groups on the precursor were sensitive to controlling the catalytic activity and stability of tri-doped reduced graphene oxide (RGO). With more O groups in the precursor (catalysts from graphene oxide), the doped heteroatom species is vulnerable involved into reaction, leading to the flaking of active sites, and ultimately decreasing the activity and stability performance. By contrast, the fewer O groups in the precursor (catalysts from RGO) created stable and active defects for oxidation activity. The interactions of O groups in the C precursor with those in the heteroatom precursor were further confirmed by DFT calculations. The findings of this research presented a new strategy for controlling the stability and performance of the resulting catalysts. Graphic Abstract DEME-TFSI-coated GO and GN are first formed via covalent bonds through functional groups (O groups) on the graphene surface, non-covalent ionic bonds, and π–π stacking interactions. In this step, N, S, and F groups are weakly bonded with graphene. Stable pyridine and pyrrole structures could then be generated via dehydration and decarbonylation under a controlled temperature and pressure in the hydrothermal process. Finally, cyclization rearrangement is carried out to form graphite with a tri-doped structure.
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AICHE JOURNAL
·
2021
·Vol. 67
·期 8
·e17252
·
IF 3.993
Hu Li,Zisheng Zhang,Guanlun Sun,Suli Liu,Liangcheng An,Xingang Li,Hong Li,Xin Gao
As an attractive alternative technology for the separation of long chain olefin and paraffin, a novel silver-based deep eutectic solvent (Ag-DES) was prepared and utilized for 1-octene/n-octane separations. Comprehensive reactive extraction separation experiments were performed to highlight the Ag-DES concentration and operating temperature discriminations using compounds with different ratio of 1-octene/n-octane. The novel Ag-DES showed optimal separation performance regarding 1-octene/n-octane and possessed the highest levels separation selectivity in the range 3.57–16.11 with excellent circulation stability in our best knowledge. Furthermore, FT-Raman measurements and quantum chemistry calculation were performed to elucidate the interaction mechanism of Ag-DES in the separation of 1-octene and n-octane, which revealed that both chemical complexation and strong physical attraction existed in the complex of Ag-DES and 1-octene rather than n-octane. A practical process was proposed for the separation of olefin and paraffin, which indicates that an advanced separation technology could largely reduce the energy consumption. This study lends important insight for the development of Ag-DES reactive extraction separation process for the energy-efficient long chain α-olefins purification from F-T synthesis products.
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FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Yupeng Lin,Fengping An,Hong He,Fang Geng,Hongbo Song,Qun Huang
In this study, the structural and rheological properties of pectin extracted by high-speed shearing (HSS–P) at 50 °C ultrapure water and conventional heating methods (CE50-P and CE90-P) from passion fruit ( Passiflora edulis f. flavicarpa ) peel were evaluated and compared. The HSS-P has loose, honeycomb-like surface morphology, lower molecular weight and higher polydispersity index compared to CE50-P and CE90-P. Monosaccharide composition indicated that HSS-P had more side chains, which were caused by its highly branched molecular structure, short main chains and a high molar ratio of RG-I in the molecular configuration. FTIR spectra showed that high-speed shearing led to a decrease in the linearity of the main chain of the pectin molecule. NMR spectra indicated that the molecular structure of HSS-P was a linear chain of homogalacturonan (HG) inserted into rhamnogalacturonan I (RG-I) possibly with arabinans and galactans as the principal side chains. Furthermore, the effects of pectin concentration, sucrose , CaCl 2 and pH on the rheological properties of different pectins were investigated. All pectin exhibited pseudoplastic fluid behavior at different environmental conditions. The HSS-P samples showed the greatest increase in the apparent viscosity and viscoelasticity in the presence of sucrose (60%–100%) and CaCl 2 (0.1–0.2 g/100 mL), pH changes and increased pectin concentration. The HSS-P was easily combined with the co-solutes and its viscoelasticity showed stronger stability under temperature changes. In conclusion, the high-speed shearing results in a unique molecular structure that contributed to the excellent rheological properties of pectin. Thus providing theoretical guidance for further application of high-speed shearing in extracting pectin.
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CHEMOSPHERE
·
2020
·
IF 5.778
Xiaobo Min,Mingfei Zhu,Yingjie He,Yunyan Wang,Haoyu Deng,Sheng Wang,Linfeng Jin,Haiying Wang,Liyuan Zhang,Liyuan Chai
Electro-adsorption is attracting increasing attention as an emerging technology for removing ionic species from water but suffer from low selectivity . In this work, a bismuth/reduced graphene oxide nanocomposite electrode was fabricated by a facile and green method. Based on this material, an electrode with improved selectivity by electrochemistry deionization system was successfully fabricated. The bismuth nanoparticles were uniformly covered with reduced graphene oxide plates and the ratio of Bi on the whole materials is 79.56%. Bismuth/reduced graphene oxide showed ions selectivity in the order of Cl − > F − ≫ SO 4 2 − . The average Cl − removal capacity can reach as high as 62.59 mg g −1 . Moreover, bismuth/reduced graphene oxide electrodes have good regeneration performance. Typically, in the 10 adsorption-desorption multicycles, the salt absorption/desorption capacity of the hybrid capacitive deionization system is stable and reversible. This research opened a hopeful window to design and synthesize effective materials to selectively remove the ionic species to purify the water.
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