ANALYTICA CHIMICA ACTA
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2025
·
IF 6
Chenyang Wang,Tiantian Guo,Rong Li,Shujuan Ma,Yinmao Wei,Junjie Ou
Background The traditional chiral stationary phases (CSPs) preparation process involved two main parts: matrix microsphere structure regulation and surface bonding of chiral selector, demonstrating cumbersome preparation steps and long time consumption, while the currently spherical chiral hybrid CSPs could easily solve this problem by using chiral selectors as silicone precursors to participate in hybridization of CSPs directly, the preparation efficiency would be significantly improved, while the development of chiral hybrid core-shell silica CSPs with excellent monodispersity, uniform shell thickness and suitable pore structure was a challenging problem. Results In this work, an oil-water bi-phase method was been precisely designed to fabricate the sub-2 μm core-shell silica microspheres with quinine-containing hybrid fibrous shell (SiO 2 @SiO 2 - t -BuCQN) in one-pot process, the hybrid shell thickness and specific surface area were measured about 171 nm and 120.4 m 2 g −1 . By selecting appropriate oil solvents in the oil phase, the average pore size of these hybrid SiO 2 @SiO 2 - t -BuCQN microspheres could be carefully regulated in 7.5–10.6 nm. Most importantly, when the dosage of quinine silicone precursor did not exceed 30 mol%, the hybrid microspheres would exhibit excellent monodispersity without amorphous nanoparticles. These SiO 2 @SiO 2 - t -BuCQN as CSPs, performed lower chromatographic back pressure (13.8 MPa at 1 mL min −1 ) and efficient separation ability for six amino acid racemates, in which demonstrated typical reversed-phase retention and weak anion exchange chromatography mechanism. Significance The oil-water bi-phase method was simple, efficient and practical, that could significantly shorten the preparation procedures of hybrid core-shell silica CSPs. The advanced monodisperse silica stationary phases could be facilely prepared by this method for high-throughput separation and analysis of complex tasks.
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高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Bo Ma,Hailin Ma,Baixin Gu,Kangling Xu,Pei Cao,Xiaotong Wu,Tiantian Qi,Rou Wen,Tianze Sun,Junyu Su,Hanwen Cheng,Yujie Wang,Shiyan Liu,Yiyang Liu,Qiuan Xiang,Zhongyu Xu,Yuanchao Zhu,Jian Weng,Fei Yu
Persistent reactive oxygen species (ROS) stress, excessive inflammation, insufficient angiogenesis, and inadequate neurogenesis severely hinder peripheral nerve repair and remain inadequately addressed by current biomaterials. To overcome these challenges, we developed a multifunctional conduit system with a facile and efficient assembly process. Specifically, 4-amino-3-fluorophenylboronic acid (AFBA) functionalized hyaluronic acid (HP) was crosslinked with tannic acid (TA) through boronate ester bonds to obtain a dynamic hydrogel (HT), during which VEGF was encapsulated. Subsequently, HT was physically blended with NGF-encapsulated Gelatin Methacryloyl (GelMA) microspheres (MS). In the early stage of nerve injury, the ROS-rich inflammatory microenvironment triggered the oxidative cleavage of boronate ester bonds, resulting in rapid degradation of the HT hydrogel and the corresponding release of TA and VEGF. Subsequently, the released TA, enriched with phenolic hydroxyl groups, effectively continued to scavenge excessive ROS and promoted macrophage polarization toward the M2 phenotype, while VEGF facilitated early neovascularization at the injury site. Additionally, the UV-crosslinked MS ensured the sustained release of encapsulated NGF, thereby providing long-term neurotrophic support that enhanced Schwann cell activity and promoted axonal elongation. The in vivo application of the HT/MS hydrogel with chitin conduits demonstrated effective nerve regeneration across a 10-mm defect in rats.
研究领域:
生物材料
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组织工程
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神经科学
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高分子化学
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生物医学工程
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高影响因子
Aggregate
·
2023
·Vol. 4
·期 5
·e351
·
IF 18.8
Zixiang He,Su Shen,Gong Zhang,Tengfei Miao,Xiaoxiao Cheng,Zhao Wang,Qingping Song,Wei Zhang
The preparation of monodisperse azobenzene (Azo) polymer microspheres by dispersion polymerization was reported. The photo-induced fusion of Azo polymer microspheres was successfully achieved during the process of reversible trans - cis - trans photoisomerization of Azo units, and induced various unique “microsphere molecular” clusters or “microsphere polymers”. Encouraged by this interesting phenomenon, microsphere clusters of different topological structures were stabilized by the in situ acetal cross-linking chemistry. The photo-induced fusion polymerization of monodisperse polymer microspheres provides a new strategy for designing photo-responsive clusters and allows for control over the mechanical properties of microspheres with high spatiotemporal resolution.
研究领域:
材料科学
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聚合物化学
·
光化学
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高影响因子
Polymers
·
2025
·Vol. 17
·期 19
·2592
·
IF 4.9
Yingying Yu,Cheng Yang,Yaxi Zhang,Linjia Wang,Hong Wang,Fandong Meng,Fanyi Meng,Tao Wang,Zhenmin Luo
Synergistic improvements in lightweight design, flexibility, and energy absorption efficiency are a long-standing issue of flexible impact protection materials. This study proposed a multi-scale synergistic enhancement method, where nano-scale nano-silica (NS) and micro-scale carbon hollow microspheres (CHMs) were adopted to enhance the dynamic impact property of the flexible polydimethylsiloxane (PDMS). The mechanical behavior of the as-prepared composites under a broad range of strain rates (10−3–4500 s−1) was systematically investigated. For the composite with 1 wt.% NS and 3 wt.% CHM, the compressive strength under quasi-static conditions reached 39.73 MPa, representing a 67% improvement over the pure PDMS. Under dynamic impact (4500 s−1strain rate), the force transmits through the NS nanonet with the energy absorbed through crushing of the CHM with a hollow structure, which elevates the specific energy absorption properties to 40.18 J/g (for the composite with 1 wt.% NS and 3 wt.% CHM), which owns a 179% enhancement compared to the composite with only 4 wt.% CHM. This work established a novel method for a hierarchical “rigid-flexible energy absorption” structural design of lightweight impact-resistant materials.
研究领域:
材料科学
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高分子复合材料
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力学性能
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生物医学工程
·
防护材料
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PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Juan Han,Li Wang,Lei Wang,Chunmei Li,Yanli Mao,Yun Wang
Bromelain is an important industrial proteolytic enzyme which has great commercial value and is of wide application in food, beverage, tenderization, cosmetic, textile and pharmaceutical industries. In this work, the core-shell-shell magnetic polymeric microspheres (Fe 3 O 4 @SiO 2 @P(NIPAM-co-AIM)/Ni 2+ ) composed of an SiO 2 -coated Fe 3 O 4 magnetite core and a Ni 2+ -immobilized cross-linked poly (N-isopropyl acrylamide-co-propylimidazole) (NIPAM-co-AIM) shell were synthesized via distillation-precipitation polymerization. The Ni 2+ cations in the magnetic polymeric microspheres shell provided docking sites for histidine and the microspheres exhibited excellent performance in the separation of bromelain with a binding capacity as high as 198 mg/g, and the recovery of enzyme activity could achieve 80%. It was found that the microspheres showed excellent performance for separation and purification of bromelain from the crude extract of pineapple peel, moreover the enzyme structure remained unchanged before and after elution process.
研究领域:
生物化学
·
材料科学
·
酶工程
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高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2019
·
IF 3.372
Zhourong Xiao,Fang Hou,Yueting Li,Rongrong Zhang,Guoqiang Shen,Li Wang,Xiangwen Zhang,Qingfa Wang,Guozhu Li
Fe 2 O 3 nanoparticles is confined in the mesoporous shell of N-doped carbon microsphere for oxygen reduction reaction (ORR). A series of hollow catalysts together with corresponding control samples have been constructed, characterized and evaluated. The structure, composition and state of the as-prepared catalysts were characterized by Scanning electron microscope, Transmission electron microscope, X-Ray diffraction, X-Ray photoelectron spectroscopy, Raman and nitrogen adsorption-desorption. The electrochemical activity and selectivity of Fe 2 O 3 -based catalyst toward the 4e − pathway has been greatly improved (E onset of −50 mV, E 1/2 of −150 mV and average electron transfer number of 3.87). The current density is 18.5% higher than that of commercial 20 wt% Pt/C. Density functional theory calculations reveal that the confinement of Fe 2 O 3 nanoparticles in the interlayer of N-doped graphite can significantly promote the activity of N-doped graphitic surface in alkaline media for ORR, due to facilitated O 2 adsorption and reduced energy of OOH formation which is the rate-determining step of ORR. Such a confinement strategy provides a general and effective route to push the limit of transition metal oxides to a higher level for ORR and realize the controllable construction of highly active non-noble-metal catalysts with well-defined mechanism understanding.
研究领域:
材料科学
·
电化学
·
催化
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高影响因子
MATERIALS & DESIGN
·
2026
·
IF 8.2
Siyi Wang,Lijuan Chen,Wenfang Du,Fubing Xiao,Qiang Xi
Intervertebral disc degeneration (IVDD)-associated low back pain represents a substantial global health challenge with profound socioeconomic consequences. The dysregulated immune microenvironment in degenerated discs creates a hostile milieu that suppresses tissue repair. In this study, we engineered a hierarchical delivery platform where salidroside (SAL)-functionalized Prussian blue analogues (PBA) were electrostatically assembled into gelatin methacryloyl (GelMA) hydrogel microspheres. The system enables spatiotemporally controlled SAL release by combining PBA-mediated molecular loading with GelMA microsphere-based sustained delivery reservoirs. These functionalized hydrogel microspheres serve as fundamental regulatory units for the IVDD immune microenvironment, enabling minimally invasive treatment of IVDD. In vitro experiments revealed that functionalized hydrogel microspheres effectively promoted the activation of macrophages towards the reparative sentinel M2 phenotype, scavenged reactive oxygen species (ROS), and improved extracellular matrix (ECM) metabolism. In vivo studies further revealed that the functionalized hydrogel microspheres significantly restored intervertebral disc height, water content, and tissue structure in a rat IVDD model, while also enhancing ECM repair and suppressing inflammation. This study provides a novel therapeutic strategy for immunomodulation and tissue repair in degenerative disc diseases, with potential for future clinical translation.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-20
·
IF 10.2
Tang Yingchuang,Zhang Kai,Zhou Hongyou,Zhang Chenchen,Liu Zixiang,Chen Hao,Li Hanwen,Chen Kangwu
Background Cell transplantation has been demonstrated as a promising approach in tissue regeneration. However, the reactive oxygen species (ROS) accumulation and inflammation condition establish a harsh microenvironment in degenerated tissue, which makes the transplanted cells difficult to survive.Methods In this study, we constructed a keep-charging hydrogel microsphere system to enable cells actively proliferate and function in the degenerated intervertebral disc. Specifically, we combined Mg2+ to histidine-functionalized hyaluronic acid (HA-His-Mg2+) through coordination reaction, which was further intercrossed with GelMA to construct a double-network hydrogel microsphere (GelMA/HA-His-Mg2+, GHHM) with microfluidic methods. In vitro, the GHHM loaded with nucleus pulposus cells (GHHM@NPCs) was further tested for its ability to promote NPCs proliferation and anti-inflammatory properties. In vivo, the ability of GHHM@NPCs to promote regeneration of NP tissue and rescue intervertebral disc degeneration (IVDD) was evaluated by the rat intervertebral disc acupuncture model.Results The GHHM significantly enhanced NPCs adhesion and proliferation, providing an ideal platform for the NPCs to grow on. The loaded NPCs were kept active in the degenerative intervertebral disc microenvironment as charged by the Mg2+ in GHHM microspheres to effectively support the loaded NPCs to reply against the ROS-induced inflammation and senescence. Moreover, we observed that GHHM@NPCs effectively alleviated nucleus pulposus degeneration and promoted its regeneration in the rat IVDD model.Conclusion In conclusion, we constructed a keep charging system with a double-network hydrogel microsphere as a framework and Mg2+ as a cell activity enhancer, which effectively maintains NPCs active to fight against the harsh microenvironment in the degenerative intervertebral disc. The GHHM@NPCs system provides a promising approach for IVDD management.Graphical Abstract
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 40
·18659-18670
·
IF 3.3
Zhentao Wang,Waheed Iqbal,Jingjing Wang,Ninghui Chang,Chuanguang Qin
Degrading dye waste using inexpensive synthetic photocatalysts is intriguing since it provides the dual advantage of treating water and managing waste simultaneously. There is considerable interest in the construction of heterojunctions for their efficiency in using solar energy for the degradation of wastewater contaminants and enhancing the solar conversion efficiency. Herein, BiVO4 microspheres and 2D g-C3N4 nanosheets were used to synthesize a direct Z-scheme BiVO4/g-C3N4 heterojunction through a hydrothermal calcination approach. The distinct 0D/2D intimate contact heterostructure displayed a high interfacial area and broader visible-light absorption range together with high photoexcited charge pair mobility. Optimizing the content of the BiVO4 microspheres in the heterostructure led to the maximum efficiency for the visible-light photodegradation of rhodamine B (RhB), which was about 12.5 and 2.4 times higher than that for pure BiVO4 and g-C3N4, respectively. Moreover, the quenching effects of various scavengers demonstrated that ˙O2− and ˙OH radicals were the principal active species responsible for ameliorating the photocatalytic performance for RhB degradation. The demonstrated strategy for boosting the photocatalytic performance with Z-scheme systems may help the advancement of high-performance photocatalysts for sustainable and efficient pollutant degradation and energy applications.
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高影响因子
FOOD CONTROL
·
2015
·
IF 2.806
Xiya Zhang,Kai Wen,Zhanhui Wang,Haiyang Jiang,Ross C. Beier,Jianzhong Shen
A rapid lateral flow fluorescent microsphere immunochromatographic test strip (FM-ICTS) assay has been developed for the detection of aflatoxin M 1 (AFM 1 ) residues in milk. For this purpose, an ultra-sensitive anti-AFM 1 monoclonal antibody (MAb) 1D3 was prepared and identified. The IC 50 value of the MAb 1D3 for AFM 1 was 23 ng/L, and the cross-reactivity (CR) of the MAb with aflatoxin M 2 , aflatoxin B 1 , aflatoxin B 2 , aflatoxin G 1 and aflatoxin G 2 was 18%, 9%, <0.15%, 43% and <0.3%, respectively. The MAb was covalently conjugated with carboxylate-modified FMs, and the FMs were used as the label in a competitive immunochromatography assay. Using a moderate hapten-to-protein coupling ratio (˜2) in the coating antigen resulted in improved immunoassay sensitivity. Under optimal conditions, the IC 50 value of the assay was 36.3 ng/L with the limit of detection (LOD) of 4.4 ng/L in milk samples using a fluorescence reader, and the recoveries of AFM 1 in spiked milk samples ranged from 76.6 to 110.8% with coefficient of variation (CV) of 4–14.7%. The whole procedure could be completed within 30 min. The results demonstrated that the FM-ICTS assay is easy to conduct, rapid, highly sensitive and specific for the detection of AFM 1 residues in milk.
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Biomaterials Science
·
2023
·
IF 6.6
Fan Leng,Taotao Li,Tongfei Li,Cong Xie,Xulin Jiang
Timely control of coagulopathy bleeding can effectively reduce the probability of wound infection and mortality. However, it is still a challenge for microsphere hemostatic agents to achieve timely control of coagulopathy bleeding. In this work, the CCM-g-AA@DA hemostatic agent based on carboxymethyl chitin microspheres, CCM, was synthesized using electron beam irradiation-induced grafting polymerization of acrylic acid and coupling with dopamine. Irradiation grafting endowed the microspheres with excellent adsorption performance and a rough surface. The microspheres showed a strong affinity to blood cells, especially red blood cells. The maximum adsorption of red blood cells is up to approximately 100 times that of the original microspheres, the CCM. The introduction of dopamine increased the tissue adhesion of the microspheres. At the same time, the microspheres still possessed good blood compatibility and biodegradability. Furthermore, the CCM-g-AA@DA with Fe3+ achieved powerful procoagulant effects in the rat anticoagulant bleeding model. The bleeding time and blood loss were both reduced by about 90% compared with the blank group, which was superior to that of the commercially available collagen hemostatic agent Avitene™. In summary, the CCM-g-AA@DA hemostatic agent shows promising potential for bleeding control in individuals with coagulation disorders.
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Liwen Hu,Zhenzhen Chen,Ting Li,Xianlong Ye,Qi Luo,Weihua Lai
Antibodies and labels were typically non-oriented conjugated in conventional immunochromatographic assays (ICAs). In this work, a C-terminal cysteine-tagged recombinant protein A (rPA) was conjugated in an oriented manner onto aggregation-induced emission fluorescence microsphere (AIEFM). The Fc fragment of anti-enrofloxacin monoclonal antibody (anti-ENR mAb) was then conjugated onto the rPA. The resulting oriented mAb-AIEFM probe was used in an ENR-ICA for the rapid detection of ENR , a widely abused animal drug. The ENR-ICA with the oriented probe saved 66.7% of anti-ENR mAb and 25% of ENR-bovine serum albumin, and had a limit of detection of 0.035 ng/mL, compared with 0.079 ng/mL for the non-oriented probe. The corresponding linear ranges of the ENR-ICA based on the oriented and non-oriented probes were 0.25–10 ng/mL and 0.1–2.5 ng/mL, respectively. This novel ICA based on the oriented probe has the potential to be used for sensitive and rapid detection in food safety.
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高影响因子
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